ISRae1
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF452376 | ND | Rhodococcus aetherivorans | Rhodococcus aetherivorans |
DNA section
IS Length : 1116 bp
Ends
IR Length : 28/38
IRL : TGTTGCGGGTCATGACGTTGTAGACACCACCAGGGGCTGACGAAGGCGGA
IRR : TGTATGAGGCCATGACGTTGGTGACACCGGTCCGGAGTTTGCTCGCGGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATCGAACTC | ATTAGC | CAGCTGGCCG | 6 |
DNA sequence
TGTTGCGGGTCATGACGTTGTAGACACCACCAGGGGCTGACGAAGGCGGACGGGCCTTCCGACGGCAGGATGGGAAGTACCACCAACTCATCCGCCGAAC
GAAAGAACCCGTCCGTGCTCCACCGTAACGCCCCGCTGTCCGTCGAAGGTCGCCGCCGTCTCGTCGAGCGGTGCCAGACTCGTCCGCTGTCCCATGTCGC
CGCCGAGATGGGGATCTCCCGGGCCTGCGCCAGCAAATGGATGGCCCGCTACCGCGAACACGGTGAACTCGGCCTGCTCGACCGCCCCAGCGTCCCGCAC
CACCAGCCCACCGCGACCCCGGCCGAGACCGTGGCGCGGATCGAGACCCTGCGACGGAAGCACAAGTGGTCCGCGGCTCGTATCGCGCACGAGCTCGCCG
CCGACGGCATCAGCATCTCGGTACGCACCGTCAGCCGGCACCTCGCTCACCTCGGCTTGAACCGGCGCAGGTTCCTCGATCCGAACGGCGAGAACAACCG
CCAGCCACGCCGGATCATCGCCCGCTGGCCCGGCCACATGGTGCACGTCGACATCAAGAAGGTCGGCCGGATTCCCGACGGTGGTGGCTGGCGTGTCCAC
GGCAAGGGCTCGGAGCAGGCCAAGAAGGTCGAGCGCGCCAAGGCCGCCGGTGCCCGCGCCGGCTACGTCTACCTGCATTCGGCGGTCGACGGATTCTCCC
GCCTTGCCTACACCGAGGCCCTGGCGGACGAGAAAGCCGCCACGGCAATCGGGTTCATGCACCGGGCGAGAGTGTTCTTCGCCGCCCACGGCATCGTGCA
CATCCACCGGGTCGTCACCGACAACGGGTCCTGCTACCGGGCGAAGGACTTCGCGAAAGTGCTCCACGGTGGCCGGCATCAACGGATCACCCCGTACACC
CCTCGTCACAACGGGAAGGTCGAGCGCTACAACCGGATTCTGGCCGAAGAGTTCCTCTACGCCCGCGTCTGGACCAGTGAACGGCAACGCGCCCAGGCGC
TACAGGTGTGCAATGTGCACTACAACTATCATCGCCCGCATACTGCCGTCGGTAACCGGCCGCCCGCGAGCAAACTCCGGACCGGTGTCACCAACGTCAT
GGCCTCATACA
GAAAGAACCCGTCCGTGCTCCACCGTAACGCCCCGCTGTCCGTCGAAGGTCGCCGCCGTCTCGTCGAGCGGTGCCAGACTCGTCCGCTGTCCCATGTCGC
CGCCGAGATGGGGATCTCCCGGGCCTGCGCCAGCAAATGGATGGCCCGCTACCGCGAACACGGTGAACTCGGCCTGCTCGACCGCCCCAGCGTCCCGCAC
CACCAGCCCACCGCGACCCCGGCCGAGACCGTGGCGCGGATCGAGACCCTGCGACGGAAGCACAAGTGGTCCGCGGCTCGTATCGCGCACGAGCTCGCCG
CCGACGGCATCAGCATCTCGGTACGCACCGTCAGCCGGCACCTCGCTCACCTCGGCTTGAACCGGCGCAGGTTCCTCGATCCGAACGGCGAGAACAACCG
CCAGCCACGCCGGATCATCGCCCGCTGGCCCGGCCACATGGTGCACGTCGACATCAAGAAGGTCGGCCGGATTCCCGACGGTGGTGGCTGGCGTGTCCAC
GGCAAGGGCTCGGAGCAGGCCAAGAAGGTCGAGCGCGCCAAGGCCGCCGGTGCCCGCGCCGGCTACGTCTACCTGCATTCGGCGGTCGACGGATTCTCCC
GCCTTGCCTACACCGAGGCCCTGGCGGACGAGAAAGCCGCCACGGCAATCGGGTTCATGCACCGGGCGAGAGTGTTCTTCGCCGCCCACGGCATCGTGCA
CATCCACCGGGTCGTCACCGACAACGGGTCCTGCTACCGGGCGAAGGACTTCGCGAAAGTGCTCCACGGTGGCCGGCATCAACGGATCACCCCGTACACC
CCTCGTCACAACGGGAAGGTCGAGCGCTACAACCGGATTCTGGCCGAAGAGTTCCTCTACGCCCGCGTCTGGACCAGTGAACGGCAACGCGCCCAGGCGC
TACAGGTGTGCAATGTGCACTACAACTATCATCGCCCGCATACTGCCGTCGGTAACCGGCCGCCCGCGAGCAAACTCCGGACCGGTGTCACCAACGTCAT
GGCCTCATACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1042 bp | 348 aa | 70 | 1111 | + | No |
Chemistry : DDE
ORF sequence :
MGSTTNSSAERKNPSVLHRNAPLSVEGRRRLVERCQTRPLSHVAAEMGISRACASKWMARYREHGELGLLDRPSVPHHQPTATPAETVARIETLRRKHKW
SAARIAHELAADGISISVRTVSRHLAHLGLNRRRFLDPNGENNRQPRRIIARWPGHMVHVDIKKVGRIPDGGGWRVHGKGSEQAKKVERAKAAGARAGYV
YLHSAVDGFSRLAYTEALADEKAATAIGFMHRARVFFAAHGIVHIHRVVTDNGSCYRAKDFAKVLHGGRHQRITPYTPRHNGKVERYNRILAEEFLYARV
WTSERQRAQALQVCNVHYNYHRPHTAVGNRPPASKLRTGVTNVMASYN
SAARIAHELAADGISISVRTVSRHLAHLGLNRRRFLDPNGENNRQPRRIIARWPGHMVHVDIKKVGRIPDGGGWRVHGKGSEQAKKVERAKAAGARAGYV
YLHSAVDGFSRLAYTEALADEKAATAIGFMHRARVFFAAHGIVHIHRVVTDNGSCYRAKDFAKVLHGGRHQRITPYTPRHNGKVERYNRILAEEFLYARV
WTSERQRAQALQVCNVHYNYHRPHTAVGNRPPASKLRTGVTNVMASYN
Blast result :
Comments
ISRae1 is 55% aa similar to IS1121. The stop Codon is in the DR.
References
1] Priefert,H., O'Brien,X.M., Lessard,P.A., Dexter,A.F., Choi,E.E., Tomic,S., Nagpal,G., Cho,J.J., Agosto,M., Yang,L., Treadway,S.L., Tamashiro,L., Wallace,M. and Sinskey,A.J. (2004) Appl. Microbiol. Biotechnol. 65 (2), 168-176.