ISNwi5
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007406 | ND | Nitrobacter winogradskyi | Nitrobacter winogradskyi Nb-255 |
DNA section
IS Length : 1003 bp
Ends
IR Length : 20/23
IRL : TGTTTGATCCCGGCATTTGATGGTGCATTATTTTCCGGAGAATGGAAGGA
IRR : TGTTCAGTCCCGGCATTTGATGGATTGGATCGATGATGAATCGATCGGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGCTGTTTG | CCTAGGTCAT | 0 |
DNA sequence
TGTTTGATCCCGGCATTTGATGGTGCATTATTTTCCGGAGAATGGAAGGATGCGCTATGGGACAGGTTCTTCACGGGAGCGCCACGACGACAGAGGCAAT
CCGTCGAGCAATACAAAATAGTGAAGAGAGCCTGAGAGCGCTGTCGAAACGCTACGGGATCAACCAGAAGACCGTCGCAAAATGGAAGAAGCGGACCTCG
GTGGCCGACCTTCCGACGGGACCGAAAGATCCGCGCTCGACGGCGCTCTCATCCGAGGAGGAGGCGATCATCGTCGCCTTCCGCAAGCATACCCTGTTGC
CGCTCGATGATTGCCTCTACGCGCTTCAGCCAACGATCCCGCACCTGTCGCGGTCTTCATTGCATCGCTGCCTGCACCGTCACGGCATCAGCCGGCTCCC
GGAGGTCGAAGGCGACAAGCCGATCAGGAAGACGTTCAGGAGCTACCCGATCGGCTACTTCCACATCGACATCGCCGAGGTGCAGACGGCTGAGGGCAAG
CTGCGCCTGTTCGTGGCCATCGACCGCACTTCGAAGTTCGCCTACGCCGAGCTCCACCAAGAGGCGGGCAAGATGGTCGCGGCTCAGTTCCTGCGCAACC
TCATCGTGGCAGTTCCCTATGCCATCCACACGGTGCTGACGGACAACGGCATCCAGTTCACCAACCACGCCCGTCACAAATATGCGTTCCACCACATCTT
CGATCGCGTCTGCGACGAGAACGGCATCGAGCACAGGCTCACCAGGATCAACCATCCCTGGACCAACGGACAGGTGGAACGGATGAACCGCACCATCAAG
GACGCCACCGTCAAGCGCTTCCACTACGACAATCATGACCAACTGCGCCGGCACCTTCAGGACTTCATCAAGGCATACAACTTCGGCCGAAGGCTGAAGA
CGCTCAAAGCGCCTCACACCCTACGAGTTCATCTGCAAACGATGGACTTCAGAGCCCGATCGATTCATCATCGATCCAATCCATCAAATGCCGGGACTGA
ACA
CCGTCGAGCAATACAAAATAGTGAAGAGAGCCTGAGAGCGCTGTCGAAACGCTACGGGATCAACCAGAAGACCGTCGCAAAATGGAAGAAGCGGACCTCG
GTGGCCGACCTTCCGACGGGACCGAAAGATCCGCGCTCGACGGCGCTCTCATCCGAGGAGGAGGCGATCATCGTCGCCTTCCGCAAGCATACCCTGTTGC
CGCTCGATGATTGCCTCTACGCGCTTCAGCCAACGATCCCGCACCTGTCGCGGTCTTCATTGCATCGCTGCCTGCACCGTCACGGCATCAGCCGGCTCCC
GGAGGTCGAAGGCGACAAGCCGATCAGGAAGACGTTCAGGAGCTACCCGATCGGCTACTTCCACATCGACATCGCCGAGGTGCAGACGGCTGAGGGCAAG
CTGCGCCTGTTCGTGGCCATCGACCGCACTTCGAAGTTCGCCTACGCCGAGCTCCACCAAGAGGCGGGCAAGATGGTCGCGGCTCAGTTCCTGCGCAACC
TCATCGTGGCAGTTCCCTATGCCATCCACACGGTGCTGACGGACAACGGCATCCAGTTCACCAACCACGCCCGTCACAAATATGCGTTCCACCACATCTT
CGATCGCGTCTGCGACGAGAACGGCATCGAGCACAGGCTCACCAGGATCAACCATCCCTGGACCAACGGACAGGTGGAACGGATGAACCGCACCATCAAG
GACGCCACCGTCAAGCGCTTCCACTACGACAATCATGACCAACTGCGCCGGCACCTTCAGGACTTCATCAAGGCATACAACTTCGGCCGAAGGCTGAAGA
CGCTCAAAGCGCCTCACACCCTACGAGTTCATCTGCAAACGATGGACTTCAGAGCCCGATCGATTCATCATCGATCCAATCCATCAAATGCCGGGACTGA
ACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 949 bp | 315 aa | 53 | 1001 | + | No |
Chemistry : DDE
ORF sequence :
MGQVLHGSATTTEAIRRAIQNSEESLRALSKRYGINQKTVAKWKKRTSVADLPTGPKDPRSTALSSEEEAIIVAFRKHTLLPLDDCLYALQPTIPHLSRS
SLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQ
FTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKAPHTLRVHLQTMDFRA
RSIHHRSNPSNAGTE
SLHRCLHRHGISRLPEVEGDKPIRKTFRSYPIGYFHIDIAEVQTAEGKLRLFVAIDRTSKFAYAELHQEAGKMVAAQFLRNLIVAVPYAIHTVLTDNGIQ
FTNHARHKYAFHHIFDRVCDENGIEHRLTRINHPWTNGQVERMNRTIKDATVKRFHYDNHDQLRRHLQDFIKAYNFGRRLKTLKAPHTLRVHLQTMDFRA
RSIHHRSNPSNAGTE
Blast result :
Comments
ISNwi5 is 49% aa similar to ISSco2. The Transposon use the first stop codon after the end of the element. The given protein is 15 aa longer.
References
1] Starkenburg,S.R., Chain,P.S., Sayavedra-Soto,L.A., Hauser,L., Land,M.L., Larimer,F.W., Malfatti,S.A., Klotz,M.G., Bottomley,P.J., Arp,D.J. and Hickey,W.J. (2006) Appl. Environ. Microbiol. 72 (3), 2050-2063.