ISGbe2
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008343 | ND | Granulibacter bethesdensis | Granulibacter bethesdensis CGDNIH1 |
DNA section
IS Length : 1352 bp
Ends
IR Length : 12/19
IRL : TGATCCCCGTGGTCTTCGCAACCCCCGGTGAATCACAACTGACTGAAATC
IRR : TGATCTGGCCCCCGTTTCACCGGACGGGTTCAAGCGGTTGCAGAAACCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTCCAGCCCA | TG | TCGTGGAGGG | 2 |
DNA sequence
TGATCCCCGTGGTCTTCGCAACCCCCGGTGAATCACAACTGACTGAAATCGCTCAACTCAATTTTCGGTCGGGCTCTAAGAAGGAGGCTTGGGGCTATCC
CCTGAGCATAGGCTTATGTCCGTGTCCGAGATCATCACCGACGGCGGTCGTCGCCGTTACTGGAGCACGCCTGAGAAGCTGAGGATCGTCGAGGAGACGC
TCGACGGTCGCGAGAGCATATCGGTGGTGGCGCGCCGCAACGGCGTGGCGCCCAACCTGCTGTACCGCTGGCGGCGACTGATGCTGGAAGGCGGGAGCGT
GGCGGTCGCCGGCGACGATGACGTGACCAGCAATCGCCAGGTCCGCGAGATGGAGACCCGCATCCGCGAACTGGAGCGCCAGCTCGGCCGCAAGACGCTG
GAGGTCGAGATTCTGAAGGAAGCGCTGGAGCGCTCGCGCCCAAAAAAAGCGAGCTTGCTCATGCACTCGCCGCTGCCGGAGACTATCCGGTGAGCCTGGT
CGCCAAGACGCTCGGGGTCGGGCGCTCGACGGTGTACGACCGCCTGACCGGCCGCACCCGGACTCGCGGGCCGTACGCCAAGGCCGACGACGCGGACCTG
CTGCCCCGCATCCGCCAGATCGCCGCGCAGCGGCCCACCTACGGCTACCGCCGCATCGCGGCGGTCCTCAATCGACAGCGGCGCGCCGATGGACTGGCGC
TGGTCAACCACAAGCGCGTCTACCGCATCATGGCGGCGGACCGCCTGCTGCTGGCGCGGCGCTATACCGAGCGGGCCGACTATGGCCATGACGGCGTCGT
GGTGGCGATCCGCTCGAACCTGCGCTGGTGCTCGGACGGCTTCGAGTTCACCTGCTGGAACGGTGAGGTCGTGCGCGGCGCCTTCATCATCGACGCCCAT
GACCGCGAGATCATCGCCTGGCGCGCGGTCGCCAATGCGGGCATCAGCGGCTCGGACGTGCGCGATATCATGCTGGAGGCCGTGGAAACCCGCTTCGGCG
GAATGCGCGCGCCCGTGCCGGTCGAGATGCTGTCCGATAACGGCTCGGCCTATACCGCCCGCGAAACCCGCACCTTTGCCCGGCAGCTGGGCCTCAAACC
CTGCTTCACCCCCGTCCGCAGCCCGCAGTCCAACGGCATCTCCGAGGCCTTCGTCCATACCCTCAAGCGCGATTACGTCCGCGTCTCGCCGCTGCCCGAT
GCATCCACCGCGTTGACATCGCTTGCCGGATGGATCGAAGACTACAACGACAACCACCCCCATTCAGGACTCAAAATGCGTTCACCGCGCGAACATCGCG
CATTGGTTTCTGCAACCGCTTGAACCCGTCCGGTGAAACGGGGGCCAGATCA
CCTGAGCATAGGCTTATGTCCGTGTCCGAGATCATCACCGACGGCGGTCGTCGCCGTTACTGGAGCACGCCTGAGAAGCTGAGGATCGTCGAGGAGACGC
TCGACGGTCGCGAGAGCATATCGGTGGTGGCGCGCCGCAACGGCGTGGCGCCCAACCTGCTGTACCGCTGGCGGCGACTGATGCTGGAAGGCGGGAGCGT
GGCGGTCGCCGGCGACGATGACGTGACCAGCAATCGCCAGGTCCGCGAGATGGAGACCCGCATCCGCGAACTGGAGCGCCAGCTCGGCCGCAAGACGCTG
GAGGTCGAGATTCTGAAGGAAGCGCTGGAGCGCTCGCGCCCAAAAAAAGCGAGCTTGCTCATGCACTCGCCGCTGCCGGAGACTATCCGGTGAGCCTGGT
CGCCAAGACGCTCGGGGTCGGGCGCTCGACGGTGTACGACCGCCTGACCGGCCGCACCCGGACTCGCGGGCCGTACGCCAAGGCCGACGACGCGGACCTG
CTGCCCCGCATCCGCCAGATCGCCGCGCAGCGGCCCACCTACGGCTACCGCCGCATCGCGGCGGTCCTCAATCGACAGCGGCGCGCCGATGGACTGGCGC
TGGTCAACCACAAGCGCGTCTACCGCATCATGGCGGCGGACCGCCTGCTGCTGGCGCGGCGCTATACCGAGCGGGCCGACTATGGCCATGACGGCGTCGT
GGTGGCGATCCGCTCGAACCTGCGCTGGTGCTCGGACGGCTTCGAGTTCACCTGCTGGAACGGTGAGGTCGTGCGCGGCGCCTTCATCATCGACGCCCAT
GACCGCGAGATCATCGCCTGGCGCGCGGTCGCCAATGCGGGCATCAGCGGCTCGGACGTGCGCGATATCATGCTGGAGGCCGTGGAAACCCGCTTCGGCG
GAATGCGCGCGCCCGTGCCGGTCGAGATGCTGTCCGATAACGGCTCGGCCTATACCGCCCGCGAAACCCGCACCTTTGCCCGGCAGCTGGGCCTCAAACC
CTGCTTCACCCCCGTCCGCAGCCCGCAGTCCAACGGCATCTCCGAGGCCTTCGTCCATACCCTCAAGCGCGATTACGTCCGCGTCTCGCCGCTGCCCGAT
GCATCCACCGCGTTGACATCGCTTGCCGGATGGATCGAAGACTACAACGACAACCACCCCCATTCAGGACTCAAAATGCGTTCACCGCGCGAACATCGCG
CATTGGTTTCTGCAACCGCTTGAACCCGTCCGGTGAAACGGGGGCCAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 402 bp | 133 aa | 92 | 493 | + | No |
Description : First part of the transposase
ORF sequence :
MGLSPEHRLMSVSEIITDGGRRRYWSTPEKLRIVEETLDGRESISVVARRNGVAPNLLYRWRRLMLEGGSVAVAGDDDVTSNRQVREMETRIRELERQLG
RKTLEVEILKEALERSRPKKASLLMHSPLPETIR
RKTLEVEILKEALERSRPKKASLLMHSPLPETIR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1002 bp | 333 aa | 322 | 1323 | + | No |
Description : Second part of the transposase
ORF sequence :
RDQQSPGPRDGDPHPRTGAPARPQDAGGRDSEGSAGALAPKKSELAHALAAAGDYPVSLVAKTLGVGRSTVYDRLTGRTRTRGPYAKADDADLLPRIRQI
AAQRPTYGYRRIAAVLNRQRRADGLALVNHKRVYRIMAADRLLLARRYTERADYGHDGVVVAIRSNLRWCSDGFEFTCWNGEVVRGAFIIDAHDREIIAW
RAVANAGISGSDVRDIMLEAVETRFGGMRAPVPVEMLSDNGSAYTARETRTFARQLGLKPCFTPVRSPQSNGISEAFVHTLKRDYVRVSPLPDASTALTS
LAGWIEDYNDNHPHSGLKMRSPREHRALVSATA
AAQRPTYGYRRIAAVLNRQRRADGLALVNHKRVYRIMAADRLLLARRYTERADYGHDGVVVAIRSNLRWCSDGFEFTCWNGEVVRGAFIIDAHDREIIAW
RAVANAGISGSDVRDIMLEAVETRFGGMRAPVPVEMLSDNGSAYTARETRTFARQLGLKPCFTPVRSPQSNGISEAFVHTLKRDYVRVSPLPDASTALTS
LAGWIEDYNDNHPHSGLKMRSPREHRALVSATA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1232 bp | 411 aa | 92 | 1323 | + | Yes |
Chemistry : DDE
ORF sequence :
MGLSPEHRLMSVSEIITDGGRRRYWSTPEKLRIVEETLDGRESISVVARRNGVAPNLLYRWRRLMLEGGSVAVAGDDDVTSNRQVREMETRIRELERQLG
RKTLEVEILKEALERSRPKKSELAHALAAAGDYPVSLVAKTLGVGRSTVYDRLTGRTRTRGPYAKADDADLLPRIRQIAAQRPTYGYRRIAAVLNRQRRA
DGLALVNHKRVYRIMAADRLLLARRYTERADYGHDGVVVAIRSNLRWCSDGFEFTCWNGEVVRGAFIIDAHDREIIAWRAVANAGISGSDVRDIMLEAVE
TRFGGMRAPVPVEMLSDNGSAYTARETRTFARQLGLKPCFTPVRSPQSNGISEAFVHTLKRDYVRVSPLPDASTALTSLAGWIEDYNDNHPHSGLKMRSP
REHRALVSATA
RKTLEVEILKEALERSRPKKSELAHALAAAGDYPVSLVAKTLGVGRSTVYDRLTGRTRTRGPYAKADDADLLPRIRQIAAQRPTYGYRRIAAVLNRQRRA
DGLALVNHKRVYRIMAADRLLLARRYTERADYGHDGVVVAIRSNLRWCSDGFEFTCWNGEVVRGAFIIDAHDREIIAWRAVANAGISGSDVRDIMLEAVE
TRFGGMRAPVPVEMLSDNGSAYTARETRTFARQLGLKPCFTPVRSPQSNGISEAFVHTLKRDYVRVSPLPDASTALTSLAGWIEDYNDNHPHSGLKMRSP
REHRALVSATA
Blast result :
Comments
ISGbe2 is 76% (ORFA) and 74% (ORFB) aa similar to IS426. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Greenberg,D.E., Porcella,S.F., Zelazny,A.M., Stock,F., Dorward,D.W., Murray,P.R. and Holland,S.M.(2006) Direct submission GenBank.