ISMaq2
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008740 | ND | Marinobacter aquaeolei | Marinobacter aquaeolei VT8 |
DNA section
IS Length : 1321 bp
Ends
IR Length : 26/30
IRL : TGTAGTAGCCCCCATTTAAACCGGACACCTTGGTAGAAGCAAAGGCCTAG
IRR : TGTAGTTGCCCCCGCTAAACCGGACACCACCTTATTCGTTTGATGCCAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCGCTCCAGC | GGCTCCTTTTC | 0 |
DNA sequence
TGTAGTAGCCCCCATTTAAACCGGACACCTTGGTAGAAGCAAAGGCCTAGGCATAGACCTAGTGCAAAGGAGTGTCCAAGGTGGAGAGAATCGAAGTCAT
AACCGGAGTGCAGCGCCGTCGCCGGTATTCCGCGCAGGAGAAAGCATTCATGGTTGCAGAGTGTGAGCAGCCAGGCATGTCGGTATCGCTTGTAGCCCGG
CGTCATGGTATATCAGCAAGTCTGTTGTTCCGTTGGAAGAAGCTGATGAAAGACGGTGGCATGTCCGCGATTGAATCCGGTGATGAGGTTGTAAGCGCAT
CCGAGGTGAAAGCCCCGAACAAGAAGGTTCGGGAGCTGGAGCGGATGTTGGGCCGCAAGACGATGGAAGCTGAAATACTCCGGGAAGCCCTGGAGGTGGC
TCAGTCAACAAAGTTGATCTCGCGCATGCCGTTGCTGCCACCGGACGATACCCTCTGAAGCGGGTTGCGGAAGTGCTGAAGGTATCGCGCTCCAATCTTC
TGGATCGTCTTCATGGTCGCCAGAAAGGCCGAAGCCCGAGATACAGAAAACAGGACGATGACCGGCATTTGCCGTTTATCCGTGAGCTGTGCGAGGCCCG
TGCTGCCAATGGCTATCGAAGGATCACCGCACGACTGAATCGGTTGCTCGCAGCCAACGACGAGCGAGTGAACCACAAGCGCGTGTACCGACTGATGAAG
CAGGATGGGCTGCTGTTACCCCGGTATACAGGGCGACCTCAAGAGCGCTGCCATAACGGCCAGGTTATTACGTTGAAGCCGGACCTTCGCTGGTGCTCGG
ATGGCTTTGAGATCCGCTGCTGGAACAAGGAGGTTGTCCGTGTAGCCTTCAGCCTGGACTGCTGTGATCGCGAGCTGATGCGCTATGTAGCGACTACTGG
TGGCATCACCGGTGAGATGGTTCAGGACCTGCTGCTGGAAAGCTTGGAATACCGGTTTGGCCAGTCAGAGCGAGTGCCTCATCCGCTGGAATGGCTGACG
GACAATGGCAGTTGCTACATTGCCAAAGAAACGCGAGCCTTCGCGTCCTCTCTGGGGTTTGTAGTGTGTACTACACCGGTGAGAAGCCCTCAGAGCAACG
GCATGGCTGAGGCCTTCGTGAAGACGTTCAAGCGGGACTATGTGTACCTGAACGATCTTCCTGATGCCGCCACGGTGATGGCCAGGTTGCCGGAATGGAT
CGAGGACTATAACCGCAGCCACCCGCACAAGGGGCTGAAAATGAAATCGCCCTGGGAGTACCGGGCGGAACTGGCATCAAACGAATAAGGTGGTGTCCGG
TTTAGCGGGGGCAACTACA
AACCGGAGTGCAGCGCCGTCGCCGGTATTCCGCGCAGGAGAAAGCATTCATGGTTGCAGAGTGTGAGCAGCCAGGCATGTCGGTATCGCTTGTAGCCCGG
CGTCATGGTATATCAGCAAGTCTGTTGTTCCGTTGGAAGAAGCTGATGAAAGACGGTGGCATGTCCGCGATTGAATCCGGTGATGAGGTTGTAAGCGCAT
CCGAGGTGAAAGCCCCGAACAAGAAGGTTCGGGAGCTGGAGCGGATGTTGGGCCGCAAGACGATGGAAGCTGAAATACTCCGGGAAGCCCTGGAGGTGGC
TCAGTCAACAAAGTTGATCTCGCGCATGCCGTTGCTGCCACCGGACGATACCCTCTGAAGCGGGTTGCGGAAGTGCTGAAGGTATCGCGCTCCAATCTTC
TGGATCGTCTTCATGGTCGCCAGAAAGGCCGAAGCCCGAGATACAGAAAACAGGACGATGACCGGCATTTGCCGTTTATCCGTGAGCTGTGCGAGGCCCG
TGCTGCCAATGGCTATCGAAGGATCACCGCACGACTGAATCGGTTGCTCGCAGCCAACGACGAGCGAGTGAACCACAAGCGCGTGTACCGACTGATGAAG
CAGGATGGGCTGCTGTTACCCCGGTATACAGGGCGACCTCAAGAGCGCTGCCATAACGGCCAGGTTATTACGTTGAAGCCGGACCTTCGCTGGTGCTCGG
ATGGCTTTGAGATCCGCTGCTGGAACAAGGAGGTTGTCCGTGTAGCCTTCAGCCTGGACTGCTGTGATCGCGAGCTGATGCGCTATGTAGCGACTACTGG
TGGCATCACCGGTGAGATGGTTCAGGACCTGCTGCTGGAAAGCTTGGAATACCGGTTTGGCCAGTCAGAGCGAGTGCCTCATCCGCTGGAATGGCTGACG
GACAATGGCAGTTGCTACATTGCCAAAGAAACGCGAGCCTTCGCGTCCTCTCTGGGGTTTGTAGTGTGTACTACACCGGTGAGAAGCCCTCAGAGCAACG
GCATGGCTGAGGCCTTCGTGAAGACGTTCAAGCGGGACTATGTGTACCTGAACGATCTTCCTGATGCCGCCACGGTGATGGCCAGGTTGCCGGAATGGAT
CGAGGACTATAACCGCAGCCACCCGCACAAGGGGCTGAAAATGAAATCGCCCTGGGAGTACCGGGCGGAACTGGCATCAAACGAATAAGGTGGTGTCCGG
TTTAGCGGGGGCAACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 375 bp | 124 aa | 84 | 458 | + | No |
Description : First part of the transposase
ORF sequence :
MERIEVITGVQRRRRYSAQEKAFMVAECEQPGMSVSLVARRHGISASLLFRWKKLMKDGGMSAIESGDEVVSASEVKAPNKKVRELERMLGRKTMEAEIL
REALEVAQSTKLISRMPLLPPDDTL
REALEVAQSTKLISRMPLLPPDDTL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 915 bp | 304 aa | 374 | 1288 | + | No |
Description : Second part of the transposase
ORF sequence :
NTPGSPGGGSVNKVDLAHAVAATGRYPLKRVAEVLKVSRSNLLDRLHGRQKGRSPRYRKQDDDRHLPFIRELCEARAANGYRRITARLNRLLAANDERVN
HKRVYRLMKQDGLLLPRYTGRPQERCHNGQVITLKPDLRWCSDGFEIRCWNKEVVRVAFSLDCCDRELMRYVATTGGITGEMVQDLLLESLEYRFGQSER
VPHPLEWLTDNGSCYIAKETRAFASSLGFVVCTTPVRSPQSNGMAEAFVKTFKRDYVYLNDLPDAATVMARLPEWIEDYNRSHPHKGLKMKSPWEYRAEL
ASNE
HKRVYRLMKQDGLLLPRYTGRPQERCHNGQVITLKPDLRWCSDGFEIRCWNKEVVRVAFSLDCCDRELMRYVATTGGITGEMVQDLLLESLEYRFGQSER
VPHPLEWLTDNGSCYIAKETRAFASSLGFVVCTTPVRSPQSNGMAEAFVKTFKRDYVYLNDLPDAATVMARLPEWIEDYNRSHPHKGLKMKSPWEYRAEL
ASNE
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1205 bp | 402 aa | 84 | 1288 | + | Yes |
Chemistry : DDE
ORF sequence :
MERIEVITGVQRRRRYSAQEKAFMVAECEQPGMSVSLVARRHGISASLLFRWKKLMKDGGMSAIESGDEVVSASEVKAPNKKVRELERMLGRKTMEAEIL
REALEVAQSTKVDLAHAVAATGRYPLKRVAEVLKVSRSNLLDRLHGRQKGRSPRYRKQDDDRHLPFIRELCEARAANGYRRITARLNRLLAANDERVNHK
RVYRLMKQDGLLLPRYTGRPQERCHNGQVITLKPDLRWCSDGFEIRCWNKEVVRVAFSLDCCDRELMRYVATTGGITGEMVQDLLLESLEYRFGQSERVP
HPLEWLTDNGSCYIAKETRAFASSLGFVVCTTPVRSPQSNGMAEAFVKTFKRDYVYLNDLPDAATVMARLPEWIEDYNRSHPHKGLKMKSPWEYRAELAS
NE
REALEVAQSTKVDLAHAVAATGRYPLKRVAEVLKVSRSNLLDRLHGRQKGRSPRYRKQDDDRHLPFIRELCEARAANGYRRITARLNRLLAANDERVNHK
RVYRLMKQDGLLLPRYTGRPQERCHNGQVITLKPDLRWCSDGFEIRCWNKEVVRVAFSLDCCDRELMRYVATTGGITGEMVQDLLLESLEYRFGQSERVP
HPLEWLTDNGSCYIAKETRAFASSLGFVVCTTPVRSPQSNGMAEAFVKTFKRDYVYLNDLPDAATVMARLPEWIEDYNRSHPHKGLKMKSPWEYRAELAS
NE
Blast result :
Comments
ISMaq2 is 78% (ORFA) aa similar to ISRso10 and 66% (ORFB) aa similar to IS426. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Edwards,K. and Richardson,P.