ISNmu3
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007614 | ND | Nitrosospira multiformis | Nitrosospira multiformis ATCC 25196 |
DNA section
IS Length : 1323 bp
Ends
IR Length : 28/34
IRL : TGTAGTAGCCCCCAATTGAACCGGACACAGATGCACTGATGACAGGAGAT
IRR : TGGAGTTGCCCCTATTAAACCGGACAGGATGCGGTTAGTAACTGGCTGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGCAATTTA | CTTTT | GGAGGAAGGA | 5 |
DNA sequence
TGTAGTAGCCCCCAATTGAACCGGACACAGATGCACTGATGACAGGAGATAGGCATATGCCTGGTGCTATGCATAGTGCTTCAGGTTTGGATCGTATTGA
AGTAGTAACCGGGGTTGAGCGCCGCAGGCGCTGGACCCTGGGTGAGAAATTAAAAGCTGTTGAGGAATCCCGCTTGCCGGGCATGAGTGTCTCGTATGTG
GCGCGAAAATATGGTATTGCTCCGAGCTTGTTATTTCGCTGGAGGAAGCTTATGGCTGAGGGCGGACAAGAAGCAGTGCGTAGTGGTGATGCGGTAGTGT
CTGCGGCCGAGGTGAAAGAGTTGAAGAAGCGGATCCGGGAGTTGGAGCGGGTTCTGGGCAAGAAAACCCTGGAGAACGAGATTCTCACGGAAGCGGTGAA
GCTTGCGCATGAAAAAAAACTGATCTCGCGCATGCCCTTGCTGCCACCGGACGATTTGCCATGAAGGCCATTGCAGACACCATGGGTGTGGCGCGCTCCC
GTCTTGTGGAACGGATGAAGCCGCAGGAAAAGTCTTTTCGTTGCCGCTACAACAAGGCTGATGATGCCTGGCTGTTGCCCTTGATCCGCGAAATTGTGGA
TTGCCGCGCGACCTACGGATACAGAAGGATTTGCGCTCTCCTTAACCGCAAACTGGAGAGAATGGGCAAGCCTGCTGTCAATCACAAGCGGGTCTATCGC
ATCATGCGCCAGAATGGCCTGCTGCTGGCCCGATATACGGGCAAACAGCGCGTGCTGTCTCATGAAGGCAAGGTCATTACCTTGCGTTCCAATTTGCGCT
GGTGTTCGGATGGTTTTGAGATTCCCTGCTGGAATGGGCAAGTAGTGCGTGTTGCCTTCGCCCTGGATTGCTGTGATCGTGAAGTCATCAGCCATGTTGC
CACCACTGGCGGCATTACTGGGGAAATGGTGCGCGATCTGATGACTGAATCCGTTGAGCGGCGCTTCGGCACCGTTGACCTGCTCCCACACAGGGTGGAG
TGGCTCAGCGACAATGGCAGCTGTTACACCGCCAGCGAGACAACTGCTTTTGCAAAGGATATGGGCTTCATCAGCTGTTTTACTCCCGTCAGAAGTCCCG
AATCCAACGGTATGGCCGAGGCCTTCGTCAAAACCTTCAAGCGGGATTACGTCTATGTCCATGATCGTCCCGACGCAAAAACCGTAATGGCGCAGCTTGC
CCAATGGTTCGAGGATTACAATGAATTCCATCCTCACAAGGGGCTGAAGATGAAGTCGCCAAGACAATTCATCCGCAGCCAGTTACTAACCGCATCCTGT
CCGGTTTAATAGGGGCAACTCCA
AGTAGTAACCGGGGTTGAGCGCCGCAGGCGCTGGACCCTGGGTGAGAAATTAAAAGCTGTTGAGGAATCCCGCTTGCCGGGCATGAGTGTCTCGTATGTG
GCGCGAAAATATGGTATTGCTCCGAGCTTGTTATTTCGCTGGAGGAAGCTTATGGCTGAGGGCGGACAAGAAGCAGTGCGTAGTGGTGATGCGGTAGTGT
CTGCGGCCGAGGTGAAAGAGTTGAAGAAGCGGATCCGGGAGTTGGAGCGGGTTCTGGGCAAGAAAACCCTGGAGAACGAGATTCTCACGGAAGCGGTGAA
GCTTGCGCATGAAAAAAAACTGATCTCGCGCATGCCCTTGCTGCCACCGGACGATTTGCCATGAAGGCCATTGCAGACACCATGGGTGTGGCGCGCTCCC
GTCTTGTGGAACGGATGAAGCCGCAGGAAAAGTCTTTTCGTTGCCGCTACAACAAGGCTGATGATGCCTGGCTGTTGCCCTTGATCCGCGAAATTGTGGA
TTGCCGCGCGACCTACGGATACAGAAGGATTTGCGCTCTCCTTAACCGCAAACTGGAGAGAATGGGCAAGCCTGCTGTCAATCACAAGCGGGTCTATCGC
ATCATGCGCCAGAATGGCCTGCTGCTGGCCCGATATACGGGCAAACAGCGCGTGCTGTCTCATGAAGGCAAGGTCATTACCTTGCGTTCCAATTTGCGCT
GGTGTTCGGATGGTTTTGAGATTCCCTGCTGGAATGGGCAAGTAGTGCGTGTTGCCTTCGCCCTGGATTGCTGTGATCGTGAAGTCATCAGCCATGTTGC
CACCACTGGCGGCATTACTGGGGAAATGGTGCGCGATCTGATGACTGAATCCGTTGAGCGGCGCTTCGGCACCGTTGACCTGCTCCCACACAGGGTGGAG
TGGCTCAGCGACAATGGCAGCTGTTACACCGCCAGCGAGACAACTGCTTTTGCAAAGGATATGGGCTTCATCAGCTGTTTTACTCCCGTCAGAAGTCCCG
AATCCAACGGTATGGCCGAGGCCTTCGTCAAAACCTTCAAGCGGGATTACGTCTATGTCCATGATCGTCCCGACGCAAAAACCGTAATGGCGCAGCTTGC
CCAATGGTTCGAGGATTACAATGAATTCCATCCTCACAAGGGGCTGAAGATGAAGTCGCCAAGACAATTCATCCGCAGCCAGTTACTAACCGCATCCTGT
CCGGTTTAATAGGGGCAACTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 408 bp | 135 aa | 57 | 464 | + | No |
Description : First part of the transposase
ORF sequence :
MPGAMHSASGLDRIEVVTGVERRRRWTLGEKLKAVEESRLPGMSVSYVARKYGIAPSLLFRWRKLMAEGGQEAVRSGDAVVSAAEVKELKKRIRELERVL
GKKTLENEILTEAVKLAHEKKLISRMPLLPPDDLP
GKKTLENEILTEAVKLAHEKKLISRMPLLPPDDLP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 898 bp | 298 aa | 412 | 1309 | + | No |
Description : Second part of the transposase
ORF sequence :
KKTDLAHALAATGRFAMKAIADTMGVARSRLVERMKPQEKSFRCRYNKADDAWLLPLIREIVDCRATYGYRRICALLNRKLERMGKPAVNHKRVYRIMRQ
NGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFEIPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDLMTESVERRFGTVDLLPHRVEWLSD
NGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAKTVMAQLAQWFEDYNEFHPHKGLKMKSPRQFIRSQLLTASCPV
NGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFEIPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDLMTESVERRFGTVDLLPHRVEWLSD
NGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAKTVMAQLAQWFEDYNEFHPHKGLKMKSPRQFIRSQLLTASCPV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1253 bp | 417 aa | 57 | 1309 | + | Yes |
Chemistry : DDE
ORF sequence :
MPGAMHSASGLDRIEVVTGVERRRRWTLGEKLKAVEESRLPGMSVSYVARKYGIAPSLLFRWRKLMAEGGQEAVRSGDAVVSAAEVKELKKRIRELERVL
GKKTLENEILTEAVKLAHEKKTDLAHALAATGRFAMKAIADTMGVARSRLVERMKPQEKSFRCRYNKADDAWLLPLIREIVDCRATYGYRRICALLNRKL
ERMGKPAVNHKRVYRIMRQNGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFEIPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDLMTESV
ERRFGTVDLLPHRVEWLSDNGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAKTVMAQLAQWFEDYNEFHPHKGLKMK
SPRQFIRSQLLTASCPV
GKKTLENEILTEAVKLAHEKKTDLAHALAATGRFAMKAIADTMGVARSRLVERMKPQEKSFRCRYNKADDAWLLPLIREIVDCRATYGYRRICALLNRKL
ERMGKPAVNHKRVYRIMRQNGLLLARYTGKQRVLSHEGKVITLRSNLRWCSDGFEIPCWNGQVVRVAFALDCCDREVISHVATTGGITGEMVRDLMTESV
ERRFGTVDLLPHRVEWLSDNGSCYTASETTAFAKDMGFISCFTPVRSPESNGMAEAFVKTFKRDYVYVHDRPDAKTVMAQLAQWFEDYNEFHPHKGLKMK
SPRQFIRSQLLTASCPV
Blast result :
Comments
ISNmu3 Is 74% (ORFA) aa similar to ISRso10 and 71% (ORFB) aa similar to IS426. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P. (2005) Direct submission GenBank.