ISOgr2
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAOT01000011 | ND | Oceanicola granulosus | Oceanicola granulosus HTCC2516 |
DNA section
IS Length : 1295 bp
Ends
IR Length : 22/28
IRL : TGGCCCCCGTTTCGACCGGACACTTGGGCATAGCCATGGAGGCTTAGGCA
IRR : TTGCCCCCGTTTCAGCGGACACTCAGGCGGTTGCGGTATGAGCTGCGATG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATTGAAC | GACCAGCT | 0 |
DNA sequence
TGGCCCCCGTTTCGACCGGACACTTGGGCATAGCCATGGAGGCTTAGGCATATGCCTGAGCACGTGCTTATGTCTGAGATAGAGATCATCACCGATGGCG
GCCGTCGGCGCCGCTGGTCCGCGAGCGAGAAGATGCGGATCGTCGAGGAGACGCTGGAGGAAGGCACCAGCATCTCGGTTGTGGCCCGCCGGAATGGCGT
TGCGCCGAACCTGCTGTATCGTTGGCGCCGCCTGATGCTGGATGGAGGAGCCGTGGCCGTGTCCGAGGATGACGACGTCACCAGCAATCGCGCCGTCCGG
CAGATGGAGGAGCGCATCCGCGAACTCGAGCGGCAGCTTGGCCGCAAGACGCTGGAGGTCGAGATCCTCAAGGAGGCGCTGGACAAGACACGCTTAAAAA
AGCGGACGTGGCTCGCGCAGTCGCAGCCGAAGGACGGTTCCCGATGAAAGTTGTGGCCGAGACTTTGGGCGTCTCCCGTTCGAACCTGCACGCCCGGCTG
AACGGGAGCGCGAAGCCGCGGCGGCGCTACCATAAGGCGCAAGACGCGGCGGTCCTGCCGTTGATCACAACGCTGGTGGCAGCGCGCCCGACCTACGGCT
ACCGCCGGATCACCGCGCTCCTGAACAGGCAGCTCCGGGCTCAAGGCGACTCGCCAGTGAACCACAAGCGCGTCTACCGGATCATGAAGGCGCACAACCT
CCTGCTGGCGCGCAGATACACCGAACGGCCAAACCTGACCCACAAAGGCAAGGTGGTCACGATGCGGTCGAATATCCGCTGGTGTTCCGACGGCTTCGAG
TTCACCTGCTGGAACGGGGACGTCATCCGCGGCGCCTTCATCATCGACGCGCACGATCGGGAGATCATCTCCTGGCGGGCCGTCGTGAACGCCGGCATCA
GTGGCTCGGACATCCGCGACATGATGCTGGAGGCGGTCGAGCGGCGGTTCGGGGACTACAGGACGCCGCACCCTGTCGAGATGCTGAGCGATAACGGCTC
ATGTTACGTCGCCAGGGAGACGCGCATCTTCGCCCGCCAGCTCGGTCTGAGGTCCTGCTACACGCCTGTGAAGAGCCCCGAGAGCAATGGCATCGCCGAG
GCGTTCGTGAAGACGCTGAAGCGCGACTACGTCCACGTCACGCCGTTGCCGGACGCCGCCACAGTCCTCAGATTGGTCGCCGGGTGGATCGAGGACTATA
ACGACAACCACCCGCACTCAGGGCTGAAGATGCGCTCGCCTCGCGAGTTCATCGCAGCTCATACCGCAACCGCCTGAGTGTCCGCTGAAACGGGGGCAA
GCCGTCGGCGCCGCTGGTCCGCGAGCGAGAAGATGCGGATCGTCGAGGAGACGCTGGAGGAAGGCACCAGCATCTCGGTTGTGGCCCGCCGGAATGGCGT
TGCGCCGAACCTGCTGTATCGTTGGCGCCGCCTGATGCTGGATGGAGGAGCCGTGGCCGTGTCCGAGGATGACGACGTCACCAGCAATCGCGCCGTCCGG
CAGATGGAGGAGCGCATCCGCGAACTCGAGCGGCAGCTTGGCCGCAAGACGCTGGAGGTCGAGATCCTCAAGGAGGCGCTGGACAAGACACGCTTAAAAA
AGCGGACGTGGCTCGCGCAGTCGCAGCCGAAGGACGGTTCCCGATGAAAGTTGTGGCCGAGACTTTGGGCGTCTCCCGTTCGAACCTGCACGCCCGGCTG
AACGGGAGCGCGAAGCCGCGGCGGCGCTACCATAAGGCGCAAGACGCGGCGGTCCTGCCGTTGATCACAACGCTGGTGGCAGCGCGCCCGACCTACGGCT
ACCGCCGGATCACCGCGCTCCTGAACAGGCAGCTCCGGGCTCAAGGCGACTCGCCAGTGAACCACAAGCGCGTCTACCGGATCATGAAGGCGCACAACCT
CCTGCTGGCGCGCAGATACACCGAACGGCCAAACCTGACCCACAAAGGCAAGGTGGTCACGATGCGGTCGAATATCCGCTGGTGTTCCGACGGCTTCGAG
TTCACCTGCTGGAACGGGGACGTCATCCGCGGCGCCTTCATCATCGACGCGCACGATCGGGAGATCATCTCCTGGCGGGCCGTCGTGAACGCCGGCATCA
GTGGCTCGGACATCCGCGACATGATGCTGGAGGCGGTCGAGCGGCGGTTCGGGGACTACAGGACGCCGCACCCTGTCGAGATGCTGAGCGATAACGGCTC
ATGTTACGTCGCCAGGGAGACGCGCATCTTCGCCCGCCAGCTCGGTCTGAGGTCCTGCTACACGCCTGTGAAGAGCCCCGAGAGCAATGGCATCGCCGAG
GCGTTCGTGAAGACGCTGAAGCGCGACTACGTCCACGTCACGCCGTTGCCGGACGCCGCCACAGTCCTCAGATTGGTCGCCGGGTGGATCGAGGACTATA
ACGACAACCACCCGCACTCAGGGCTGAAGATGCGCTCGCCTCGCGAGTTCATCGCAGCTCATACCGCAACCGCCTGAGTGTCCGCTGAAACGGGGGCAA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 315 bp | 50 aa | 133 | 447 | + | No |
Description : First part of the transposase
ORF sequence :
MPEHVLMSEIEIITDGGRRRRWSASEKMRIVEETLEEGTSISVVARRNGVAPNLLYRWRRLMLDGGAVAVSEDDDVTSNRAVRQMEERIRELERQLGRKT
LEVEILKEALDKTRLKKRTWLAQSQPKDGSR
LEVEILKEALDKTRLKKRTWLAQSQPKDGSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1005 bp | 334 aa | 273 | 1277 | + | No |
Description : Second part of the transposase
ORF sequence :
RRHQQSRRPADGGAHPRTRAAAWPQDAGGRDPQGGAGQDTLKKADVARAVAAEGRFPMKVVAETLGVSRSNLHARLNGSAKPRRRYHKAQDAAVLPLITT
LVAARPTYGYRRITALLNRQLRAQGDSPVNHKRVYRIMKAHNLLLARRYTERPNLTHKGKVVTMRSNIRWCSDGFEFTCWNGDVIRGAFIIDAHDREIIS
WRAVVNAGISGSDIRDMMLEAVERRFGDYRTPHPVEMLSDNGSCYVARETRIFARQLGLRSCYTPVKSPESNGIAEAFVKTLKRDYVHVTPLPDAATVLR
LVAGWIEDYNDNHPHSGLKMRSPREFIAAHTATA
LVAARPTYGYRRITALLNRQLRAQGDSPVNHKRVYRIMKAHNLLLARRYTERPNLTHKGKVVTMRSNIRWCSDGFEFTCWNGDVIRGAFIIDAHDREIIS
WRAVVNAGISGSDIRDMMLEAVERRFGDYRTPHPVEMLSDNGSCYVARETRIFARQLGLRSCYTPVKSPESNGIAEAFVKTLKRDYVHVTPLPDAATVLR
LVAGWIEDYNDNHPHSGLKMRSPREFIAAHTATA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1145 bp | 408 aa | 133 | 1277 | + | Yes |
Chemistry : DDE
ORF sequence :
MPEHVLMSEIEIITDGGRRRRWSASEKMRIVEETLEEGTSISVVARRNGVAPNLLYRWRRLMLDGGAVAVSEDDDVTSNRAVRQMEERIRELERQLGRKT
LEVEILKEALDKTRLKKADVARAVAAEGRFPMKVVAETLGVSRSNLHARLNGSAKPRRRYHKAQDAAVLPLITTLVAARPTYGYRRITALLNRQLRAQGD
SPVNHKRVYRIMKAHNLLLARRYTERPNLTHKGKVVTMRSNIRWCSDGFEFTCWNGDVIRGAFIIDAHDREIISWRAVVNAGISGSDIRDMMLEAVERRF
GDYRTPHPVEMLSDNGSCYVARETRIFARQLGLRSCYTPVKSPESNGIAEAFVKTLKRDYVHVTPLPDAATVLRLVAGWIEDYNDNHPHSGLKMRSPREF
IAAHTATA
LEVEILKEALDKTRLKKADVARAVAAEGRFPMKVVAETLGVSRSNLHARLNGSAKPRRRYHKAQDAAVLPLITTLVAARPTYGYRRITALLNRQLRAQGD
SPVNHKRVYRIMKAHNLLLARRYTERPNLTHKGKVVTMRSNIRWCSDGFEFTCWNGDVIRGAFIIDAHDREIISWRAVVNAGISGSDIRDMMLEAVERRF
GDYRTPHPVEMLSDNGSCYVARETRIFARQLGLRSCYTPVKSPESNGIAEAFVKTLKRDYVHVTPLPDAATVLRLVAGWIEDYNDNHPHSGLKMRSPREF
IAAHTATA
Blast result :
Comments
Genome in Progress.
ISOgr2 Is72% (ORFA) and 73% (ORFB) aa similar to IS426. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISOgr2 Is72% (ORFA) and 73% (ORFB) aa similar to IS426. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Giovannoni,S.J., Cho,J.-C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.