ISBth4

  • Family IS4
  • Group IS231
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
DQ126269 Y Bacillus thuringiensis
Bacillus thuringiensis MEX312
DNA section
IS Length : 2046 bp

Ends


IR Length : 17

IRL : CATCGCTATCAAGCTAAAGAAGTAGGTCACCCCCAAAAAGAGAAAATATG
IRR : CATCGCTATCAAGTTAAGCTTATGCAAAGTCAATTTTAGGCGAGATTGTT

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNTGGCGGTACCCNNNNNNNNNN11

DNA sequence

CATCGCTATCAAGCTAAAGAAGTAGGTCACCCCCAAAAAGAGAAAATATGTTAAATTCTCATAGATAACTATCTTATATTAATTACATAATCCAGTGTGA
TATAATAATATAAAGATATTATATTTAAAAGGGGGATTCAAATTGGTTAAAAACTTACCTTTACTAATAGTGATTTTACTACTAGGTATCAGTTCTTCTA
CATTAAGCACGAACGGATACTTTTCTCCAGTTATAGAATGGTCTTTAATGATTATAAGTATTATTTTGAACATTACTGCAGTGATAGGTTTATCATTACA
CGTCCTTGTATATCAACCTATGAAAAGATTTAATAAAAATTTAAAAGGGACCTTCAAATAAAATAATTATATTTGAGGTAACACCACATCGCTATCGAGC
TAAAAAAATGTATCCATAAAGAGAAAAAAACGCTATTCTTTCTGTAGAATCATAGGAAAGGATGGCGTTTTTTATGTCTGTTTCTGATGAATTACAACTA
TTTGCTCAAGAGATTCAACGTTTTTTATCTCCAAATACCTTACGGAATCTTGCTCGAGATGTTGGTTTCGTACAACGAACCAGTAAGTACCAAGCAAAAG
ATTTAGTCGCTTTATGTGTATGGATGAACCAAAATATCGCTACGACGTCTTTAACTCAGTTATCTAGCTGTTTAGAAGCCTCGACAGAAGTGCTCATCAG
TCCCGAGGGACTGAACCAACGATTTAATAAAGCAGCCGTCCAATTTCTACAACACATACTAGCTGAACTACTAAGCCGAAAATTGACCTCATCTATACCG
ATTTCTTCTCCATATACTTCTGTTTTTAAGCGTATTCGTATTTTAGATTCAACTGCATTTCAACTCCCTGATGTATTTTCATCGGTTTATCCAGGTGCAG
GAGGATGTAGCCATACAGCTGGGATAAAAATTCAACTTGAGTATGATTTGTTAAGTGGACAGTTCCTTCATATTCATACAGGTCCAGGTAAACAACATGA
TCGCACTTACGGCTCTTTGTGTGCCCCAACTGTGACAGCAAATGATTTATGTATCCGAGATTTAGGTTATTTTCATTTAAAAGACCTTCAATATATACAA
GATAAAGAGGCCTACTATATCTCTCGTATCAAGTCGAACACACGTATGTATCAAAAAAATCCTAACCCTGATTATTTTCAAGATGGAAGAATTAAGAAAG
GTACAGAGTATATACAGATAGATATAGAGACGTTAATGAACTCCCTTCAACCAGGACAAACGTGTGAAGTAGCTGATGCTTATGTAGGAATGAATGATAA
AGTGCCAGCTCGTGTAATTGTTCATCGATTAACAAAACAACAGCAACAAAAACGATTACAAGATCAAGCTGTAAGAGAAAAAAAGAAAGGAATGAAGTAT
TCTCCTCGTAGTAAACGACTCAGCGGTATCAATGTATATATGACAAACACCCCTACAGATATTGTCCCTATGGGACAGGTACATGACTGGTATTCTTTGC
GTTGGCAAATCGAAATTTTATTTAAAACGTGGAAGTCATTCTTTCAAATTCATCATTGTAAAAAGATAAAAGCAGAACGATTAGAGTGCCATTTATATGG
TCAATTGATTGCCATTCTACTCTGTTCTTCTATCATGTTTCAAATGCGCCAATTGCTCCTCATGAAGAAAAAACGAGAGCTTAGTGAGTATAAAGCCATA
TATATGATTAAAGACTATTTTCTTCTTCTATTCCAAACTATACAGAAAAACACCCAAGAGCTATCAAAGGTGTTACTTCGTCTGTTCAATCTTCTACAGC
AAAACGGGCGGAAATCTCATCGATATGAGAAAAAAACGGTCTTTGATATATTAGGTGTCGTTTACAATTCTATGTCTGATAATCAAGCCGCTTAATTCAA
AAAAGTGAAACCCGGTAGGGTTTATTTCGTATGCAAATCTTTCAATCACCTATACTTGCTCCTTAGAAAAAAGAAACAATCTCGCCTAAAATTGACTTTG
CATAAGCTTAACTTGATAGCGATG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
219 bp72 aa143361+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MVKNLPLLIVILLLGISSSTLSTNGYFSPVIEWSLMIISIILNITAVIGLSLHVLVYQPMKRFNKNLKGTFK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1412 bp473 aa4841895+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSVSDELQLFAQEIQRFLSPNTLRNLARDVGFVQRTSKYQAKDLVALCVWMNQNIATTSLTQLSSCLEASTEVLISPEGLNQRFNKAAVQFLQHILAELL
SRKLTSSIPISSPYTSVFKRIRILDSTAFQLPDVFSSVYPGAGGCSHTAGIKIQLEYDLLSGQFLHIHTGPGKQHDRTYGSLCAPTVTANDLCIRDLGYF
HLKDLQYIQDKEAYYISRIKSNTRMYQKNPNPDYFQDGRIKKGTEYIQIDIETLMNSLQPGQTCEVADAYVGMNDKVPARVIVHRLTKQQQQKRLQDQAV
REKKKGMKYSPRSKRLSGINVYMTNTPTDIVPMGQVHDWYSLRWQIEILFKTWKSFFQIHHCKKIKAERLECHLYGQLIAILLCSSIMFQMRQLLLMKKK
RELSEYKAIYMIKDYFLLLFQTIQKNTQELSKVLLRLFNLLQQNGRKSHRYEKKTVFDILGVVYNSMSDNQAA

 

Blast result :
Comments
The ORF B is the transposase, ISBth4 is 98% aa similar to ISBce7.ORF A is a passenger gene.
References
1] Wang, J.H., Steggles, J.R. and Ellar, D.J. (2006) Molecular characterization of virulence defects in Bacillus thuringiensis mutants (unpublished)