ISNoc1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007484 | ND | Nitrosococcus oceani | Nitrosococcus oceani ATCC 19707 |
DNA section
IS Length : 1204 bp
Ends
IR Length : 35/47
IRL : TAAGCTTCCCCCTATCTGACACAGGCTGAAGTTGGAGTTTTCTGGCAGGA
IRR : TAAGCTTCCCCGTCAATGACACAGTTCTAAAGTAGAGTTTCTGGCGTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTACGGGGC | CGCCG | CAGTTGGTAC | 5 |
DNA sequence
TAAGCTTCCCCCTATCTGACACAGGCTGAAGTTGGAGTTTTCTGGCAGGATATTACTGCGAGGAGACGAAGATGAAACGAAGCCGATTTACCGAAAGCCA
GATCGTATCGATTCTGAAAGAGGCAGATGCCGGAGCAAAGATTAAGGATCTTTGCCGCAAACACGGCATCAGCGATGCGACTTACTACAACTGGAAGGCG
AAGTACGGTGGCATGAGTACATCGGACTTGCGACGGCTGAAGGAGACGGAGGCTGAGTTGTCGCAGTACAAGAAAATGTATGCGGAGCTCGCCCACGAGA
ATTACGCACTGAAGGATCTCATTGAGAAAAAGCTCTGAGGCCACTGGATAAGCGCGAAGCGGCGCAGCACCTGGTGGCCAAACACGAGTTGAGCAAGACG
AAAGCTTGCGCGGCCGTACAACTGTCGCGCTCGAGCTGGTATCGGCAGCCGTCGCAACAGGCGGTACGAGATCAGCCTGTCATCGATGCGCTCAACACGA
TGCTCAAGAAGTACCCACGCTGGGGCTTTTGGATGTGCTATGACCGAATGCGACTGGACGGCCATGCCTGGAATCACAAGCGAGTTTATCGAGTGTACAC
AGCGATGAAGCTGAATCTTCCTCGGCGCAAGAAGCGCCGGCTACCGCAGCGTGTGCAGCAGCCGATGGTGGTTGAGGCCCGAGCCAATGCTGAATGGTCT
TTGGACTTTATGAGCGATGCGCTGTACCACGGTCGCCGCTTCCGCACGCTGAACGTGCTTGATGAGGGCGTCCGTGAAGCGCTGGATATCGTCATTGATA
CATCCATCCCTGGCGCTCGCGTTGTGCGGACCTTGGATCGTCTCATCGAATGGCGCGGCAAGCCGGATGCAATACGGGTGGACAACGGGCCAGAGTACAT
CTCCCAGGTGTTCTCGGAATGGTGCGAGAAGCACGGCATCAAACTGAACTACATCCAGCCAGGGAAACCGAACCAGAACGCGTACATCGAACGCTTCAAC
CGCACGTATCGACATGAGGTGCTGAATGCCTACGTGTTCGAATCGTTACGGCAAGTCAGAGAAATCACGCGAGCATGGATCATCGAATACAACGAAGAAC
GACCGCACGACAGCTTGGGAAAAATACCGCCTGCCATGTTCCGACGGCAGGTCGAAAACGCCAGAAACTCTACTTTAGAACTGTGTCATTGACGGGGAAG
CTTA
GATCGTATCGATTCTGAAAGAGGCAGATGCCGGAGCAAAGATTAAGGATCTTTGCCGCAAACACGGCATCAGCGATGCGACTTACTACAACTGGAAGGCG
AAGTACGGTGGCATGAGTACATCGGACTTGCGACGGCTGAAGGAGACGGAGGCTGAGTTGTCGCAGTACAAGAAAATGTATGCGGAGCTCGCCCACGAGA
ATTACGCACTGAAGGATCTCATTGAGAAAAAGCTCTGAGGCCACTGGATAAGCGCGAAGCGGCGCAGCACCTGGTGGCCAAACACGAGTTGAGCAAGACG
AAAGCTTGCGCGGCCGTACAACTGTCGCGCTCGAGCTGGTATCGGCAGCCGTCGCAACAGGCGGTACGAGATCAGCCTGTCATCGATGCGCTCAACACGA
TGCTCAAGAAGTACCCACGCTGGGGCTTTTGGATGTGCTATGACCGAATGCGACTGGACGGCCATGCCTGGAATCACAAGCGAGTTTATCGAGTGTACAC
AGCGATGAAGCTGAATCTTCCTCGGCGCAAGAAGCGCCGGCTACCGCAGCGTGTGCAGCAGCCGATGGTGGTTGAGGCCCGAGCCAATGCTGAATGGTCT
TTGGACTTTATGAGCGATGCGCTGTACCACGGTCGCCGCTTCCGCACGCTGAACGTGCTTGATGAGGGCGTCCGTGAAGCGCTGGATATCGTCATTGATA
CATCCATCCCTGGCGCTCGCGTTGTGCGGACCTTGGATCGTCTCATCGAATGGCGCGGCAAGCCGGATGCAATACGGGTGGACAACGGGCCAGAGTACAT
CTCCCAGGTGTTCTCGGAATGGTGCGAGAAGCACGGCATCAAACTGAACTACATCCAGCCAGGGAAACCGAACCAGAACGCGTACATCGAACGCTTCAAC
CGCACGTATCGACATGAGGTGCTGAATGCCTACGTGTTCGAATCGTTACGGCAAGTCAGAGAAATCACGCGAGCATGGATCATCGAATACAACGAAGAAC
GACCGCACGACAGCTTGGGAAAAATACCGCCTGCCATGTTCCGACGGCAGGTCGAAAACGCCAGAAACTCTACTTTAGAACTGTGTCATTGACGGGGAAG
CTTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKRSRFTESQIVSILKEADAGAKIKDLCRKHGISDATYYNWKAKYGGMSTSDLRRLKETEAELSQYKKMYAELAHENYALKDLIEKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 867 bp | 288 aa | 326 | 1192 | + | No |
Description : Second part of the transposase
ORF sequence :
EKALRPLDKREAAQHLVAKHELSKTKACAAVQLSRSSWYRQPSQQAVRDQPVIDALNTMLKKYPRWGFWMCYDRMRLDGHAWNHKRVYRVYTAMKLNLPR
RKKRRLPQRVQQPMVVEARANAEWSLDFMSDALYHGRRFRTLNVLDEGVREALDIVIDTSIPGARVVRTLDRLIEWRGKPDAIRVDNGPEYISQVFSEWC
EKHGIKLNYIQPGKPNQNAYIERFNRTYRHEVLNAYVFESLRQVREITRAWIIEYNEERPHDSLGKIPPAMFRRQVENARNSTLELCH
RKKRRLPQRVQQPMVVEARANAEWSLDFMSDALYHGRRFRTLNVLDEGVREALDIVIDTSIPGARVVRTLDRLIEWRGKPDAIRVDNGPEYISQVFSEWC
EKHGIKLNYIQPGKPNQNAYIERFNRTYRHEVLNAYVFESLRQVREITRAWIIEYNEERPHDSLGKIPPAMFRRQVENARNSTLELCH
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1121 bp | 373 aa | 72 | 1192 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRSRFTESQIVSILKEADAGAKIKDLCRKHGISDATYYNWKAKYGGMSTSDLRRLKETEAELSQYKKMYAELAHENYALKDLIEKKALRPLDKREAAQH
LVAKHELSKTKACAAVQLSRSSWYRQPSQQAVRDQPVIDALNTMLKKYPRWGFWMCYDRMRLDGHAWNHKRVYRVYTAMKLNLPRRKKRRLPQRVQQPMV
VEARANAEWSLDFMSDALYHGRRFRTLNVLDEGVREALDIVIDTSIPGARVVRTLDRLIEWRGKPDAIRVDNGPEYISQVFSEWCEKHGIKLNYIQPGKP
NQNAYIERFNRTYRHEVLNAYVFESLRQVREITRAWIIEYNEERPHDSLGKIPPAMFRRQVENARNSTLELCH
LVAKHELSKTKACAAVQLSRSSWYRQPSQQAVRDQPVIDALNTMLKKYPRWGFWMCYDRMRLDGHAWNHKRVYRVYTAMKLNLPRRKKRRLPQRVQQPMV
VEARANAEWSLDFMSDALYHGRRFRTLNVLDEGVREALDIVIDTSIPGARVVRTLDRLIEWRGKPDAIRVDNGPEYISQVFSEWCEKHGIKLNYIQPGKP
NQNAYIERFNRTYRHEVLNAYVFESLRQVREITRAWIIEYNEERPHDSLGKIPPAMFRRQVENARNSTLELCH
Blast result :
Comments
ISNoc1 is 85 % (ORFA) aa similar to ISLpn8 and 69% (ORFB) aa similar to ISAma2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N. and Richardson,P. (2005) Direct submission GenBank.