ISGbe1

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008343 ND Granulibacter bethesdensis
Granulibacter bethesdensis CGDNIH1
DNA section
IS Length : 1269 bp

Ends


IR Length : 20/25

IRL : TGAGCTGCCCCCTTCTGAGTGGTCCATCGACTATGACAGTCTGGATCAAG
IRR : TGAGCAGCCCCCATCGGGTGGTCCGGGTTATTAGTTAGTGCATTGTCGTC

Insertion site


Left flankDirect repeatRight flankDR Length
ACGCTGCGCGCTTGTCGTAA0

DNA sequence

TGAGCTGCCCCCTTCTGAGTGGTCCATCGACTATGACAGTCTGGATCAAGGAAGGGACGATGCATGCCTGCCAAGAAGCACAAGCCTGAGGAGATCATCG
GGAAGCTGCGTGAGGTCGAGATCATGCTGGGCCAGGGCGGGACGACCGCTGAGGCATGCCGGCGGATCGCGGTCAGCGAGCAGACCTACTACCGCTGGCG
CAAGGAGTACGGCGGCTTGAAGACGGATCAGGCGCGTCGGATGAAAGATCTGGAGAAGGAGAACCTGCGGCTGCGGCGTGCGATCTCCGACCTGACACTG
GACAAGTTGATTTTACAGGAGGCGGCGCGGGGAAACTTCTGAGCCCCGCGCGACGCCGACGCTGCATCGACCAGGTGCGAGAGGTGCTGGACATATCCGA
GCGGCGGGTGTGCCGCGTGCTGGGGCAGCACCGGTCGACACAGCGCAAGGTGCCGTGCGGGGCAGATGACGAAGAGGTGCTGACCGACGACATCGTCGCG
CTGGCCAAGCAGTACGGGCGCTACGGCTACCGCCGGGTCACCGCACTGCTGCATGCGGCGGGCTGGTCGGTGAACCATAAACGGGTGGAGCGGATCTGGC
GGCGTGAAGGCCTCAAGGTGCCGCAGAAGCAACCTAAACGTGGCCGGCTATGGTTGAACGACGGCTCATGCATCCGGCTGCGACCGGAGTATCCGGGGCA
TGTGTGGGCGTACGACTTCGTCGAAGCGCGGACGCATGACGGGCGCAAGTTCCGGATACTGACCATCATCGATGAGGCCAGCAGGGAATGCCTGGCACTG
GTCGTCGCGCGCCAGCTTCGACACGAAGACGTGCTGGCAGCGCTTGCCGACCTGTTCATCGCGAGGGGTCCGCCCGCGCATATCAGATCGGATAACGGCA
GCGAGTTCATCGCTACCGCGGTTCAGAAATGGCTTGGGCAGGTTGGCGTGAAGACGCTGTACATCGCGCCGGGTTCGCCGTGGGAGAATGGCTACAACGA
GAGCTTCAACGGCTCGCTGCGCGATGAGCTGCTCAACGGCGAGATTTTCTACAGCCTCGCTGAAGCAAGAGTACTGATCGAGGCGTGGCGCCGTCACTAC
AACACCGTCCGGCCGCACAGCAGCCTGGGCTACCGGCCGCCCGCACCACAAGCGGCGACACCGCCATTGCCGGCCTCCGGTTCCGCTTCGCTCCACCTAC
GACCGGCAATGGCGGCGGAGACGACAATGCACTAACTAATAACCCGGACCACCCGATGGGGGCTGCTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
279 bp92 aa64342+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPAKKHKPEEIIGKLREVEIMLGQGGTTAEACRRIAVSEQTYYRWRKEYGGLKTDQARRMKDLEKENLRLRRAISDLTLDKLILQEAARGNF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1083 bp360 aa1531235+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GMPADRGQRADLLPLAQGVRRLEDGSGASDERSGEGEPAAAACDLRPDTGQVDFTGGGAGKLLSPARRRRCIDQVREVLDISERRVCRVLGQHRSTQRKV
PCGADDEEVLTDDIVALAKQYGRYGYRRVTALLHAAGWSVNHKRVERIWRREGLKVPQKQPKRGRLWLNDGSCIRLRPEYPGHVWAYDFVEARTHDGRKF
RILTIIDEASRECLALVVARQLRHEDVLAALADLFIARGPPAHIRSDNGSEFIATAVQKWLGQVGVKTLYIAPGSPWENGYNESFNGSLRDELLNGEIFY
SLAEARVLIEAWRRHYNTVRPHSSLGYRPPAPQAATPPLPASGSASLHLRPAMAAETTMH

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1172 bp390 aa641235+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPAKKHKPEEIIGKLREVEIMLGQGGTTAEACRRIAVSEQTYYRWRKEYGGLKTDQARRMKDLEKENLRLRRAISDLTLDKLILQEAARGKLLSPARRRR
CIDQVREVLDISERRVCRVLGQHRSTQRKVPCGADDEEVLTDDIVALAKQYGRYGYRRVTALLHAAGWSVNHKRVERIWRREGLKVPQKQPKRGRLWLND
GSCIRLRPEYPGHVWAYDFVEARTHDGRKFRILTIIDEASRECLALVVARQLRHEDVLAALADLFIARGPPAHIRSDNGSEFIATAVQKWLGQVGVKTLY
IAPGSPWENGYNESFNGSLRDELLNGEIFYSLAEARVLIEAWRRHYNTVRPHSSLGYRPPAPQAATPPLPASGSASLHLRPAMAAETTMH

 

Blast result :
Comments
ISGbe1 is 96% (ORFA) and 91% (ORFB) aa similar to ISSpma1 . The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Greenberg,D.E., Porcella,S.F., Zelazny,A.M., Stock,F., Dorward,D.W., Murray,P.R. and Holland,S.M.(2006) Direct submission GenBank.