ISDsp2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007356 | ND | Dehalococcoides sp. | Dehalococcoides sp. CBDB1 |
DNA section
IS Length : 1210 bp
Ends
IR Length : 24/34
IRL : TGACCTGACCCCCTAAAACGAGTCCAGTCTTAATGTTAGACTGTGACCGA
IRR : TGACCTGCCCCCAATAGTTATACCACTTCCTAACTCGTCATCATAGTTAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGTTCGTAT | GGGGGCTATG | 0 |
DNA sequence
TGACCTGACCCCCTAAAACGAGTCCAGTCTTAATGTTAGACTGTGACCGAAAAGAAAGGGGACTAGATGCCTAGAAAAGCTTTTACCGCGGAACAGATCA
TCAACAAGCTTCGTGAAGCCGAGCTGATTCTGAATCAGGGCACAACGGTGCCTGAAGCCTGCCGAAAGATTAGAGTTACCGATCAGACCTACTATCGATG
GTGCAAGGAATATGGCGGCTTAAACATCGACCAGGCCAAGCGCCTTAAGGACCTGGAAAAAGAAAACGTCCAGCTAAAGCGCCTTGTGGCCGATCTTTCA
CTCGATAAGGCCATATTGAAGGAGGCGTCCCGGGGAAACTCCTAAGCCCGTCCCAAAGGCGACAAGTGATAGCCAAAGTGCGTATCAGCCTTGGAATCTC
TGAAAGACGGGCTTGTCAGGTGCTGGGTCAAGCCAGATCAACACAGCGCCGTAAAGAATCGATTACCGACGACGAAGCGAAGCTTACGCTACGAATAATT
GAACTGGCCAGCAAATACGGACGATACGGGTATCGGAGAATCACGGCGATTTTGCAAATAGCTGGTTGGAGAGTGAATCACAAAAGAGTCGAACGGATCT
GGAGAAAGGAAGGGCTGAAGGTGCCCCAGAAGCAACCCAAGCGCGGTAGACTATGGCTCAACGATGGATCATGTATCCGCCTCCGGCCGGAGTATAAGGA
CCATGTCTGGAGTTATGATTTCATGGTAGACCGCACGGCTAACGGCAGGGCTTTCAAGACACTGAATATTATTGATGAATATACCCGGGTATGTTTAAAA
ATCAAAGTTAATCGTAAGATCAAGAGTCAGGATGTAATCGACCAACTGTATGATCTGTTTATCTTTCGGGGAATTCCTGAGTATATCCGCTCGGATAATG
GGCCGGAGTTTACGGCTAAAGCAGTGCGCAAATGGCTGAGCCGGTTAGGAGTAAAGACGCTTTTTATTGAGCCCGGCAGTCCCTGGGAAAATGGGTATAT
TGAATCCTTTAACGGCAAGCTGCGGGATGAACTATTGGATCGGGAGATCTTTACTACACTGGAAGAAGCTAAAGTATTGATAGAGCAATGGAGGAGGGAA
TATAATCAAGTACGCCCGCATAGCTCTCTTGGATACAGACCACCAGCACCGGAGGCTGTGTTAACTATGATGACGAGTTAGGAAGTGGTATAACTATTGG
GGGCAGGTCA
TCAACAAGCTTCGTGAAGCCGAGCTGATTCTGAATCAGGGCACAACGGTGCCTGAAGCCTGCCGAAAGATTAGAGTTACCGATCAGACCTACTATCGATG
GTGCAAGGAATATGGCGGCTTAAACATCGACCAGGCCAAGCGCCTTAAGGACCTGGAAAAAGAAAACGTCCAGCTAAAGCGCCTTGTGGCCGATCTTTCA
CTCGATAAGGCCATATTGAAGGAGGCGTCCCGGGGAAACTCCTAAGCCCGTCCCAAAGGCGACAAGTGATAGCCAAAGTGCGTATCAGCCTTGGAATCTC
TGAAAGACGGGCTTGTCAGGTGCTGGGTCAAGCCAGATCAACACAGCGCCGTAAAGAATCGATTACCGACGACGAAGCGAAGCTTACGCTACGAATAATT
GAACTGGCCAGCAAATACGGACGATACGGGTATCGGAGAATCACGGCGATTTTGCAAATAGCTGGTTGGAGAGTGAATCACAAAAGAGTCGAACGGATCT
GGAGAAAGGAAGGGCTGAAGGTGCCCCAGAAGCAACCCAAGCGCGGTAGACTATGGCTCAACGATGGATCATGTATCCGCCTCCGGCCGGAGTATAAGGA
CCATGTCTGGAGTTATGATTTCATGGTAGACCGCACGGCTAACGGCAGGGCTTTCAAGACACTGAATATTATTGATGAATATACCCGGGTATGTTTAAAA
ATCAAAGTTAATCGTAAGATCAAGAGTCAGGATGTAATCGACCAACTGTATGATCTGTTTATCTTTCGGGGAATTCCTGAGTATATCCGCTCGGATAATG
GGCCGGAGTTTACGGCTAAAGCAGTGCGCAAATGGCTGAGCCGGTTAGGAGTAAAGACGCTTTTTATTGAGCCCGGCAGTCCCTGGGAAAATGGGTATAT
TGAATCCTTTAACGGCAAGCTGCGGGATGAACTATTGGATCGGGAGATCTTTACTACACTGGAAGAAGCTAAAGTATTGATAGAGCAATGGAGGAGGGAA
TATAATCAAGTACGCCCGCATAGCTCTCTTGGATACAGACCACCAGCACCGGAGGCTGTGTTAACTATGATGACGAGTTAGGAAGTGGTATAACTATTGG
GGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 67 | 345 | + | No |
Description : First part of the transposase
ORF sequence :
MPRKAFTAEQIINKLREAELILNQGTTVPEACRKIRVTDQTYYRWCKEYGGLNIDQAKRLKDLEKENVQLKRLVADLSLDKAILKEASRGNS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 873 bp | 290 aa | 309 | 1181 | + | No |
Description : Second part of the transposase
ORF sequence :
GHIEGGVPGKLLSPSQRRQVIAKVRISLGISERRACQVLGQARSTQRRKESITDDEAKLTLRIIELASKYGRYGYRRITAILQIAGWRVNHKRVERIWRK
EGLKVPQKQPKRGRLWLNDGSCIRLRPEYKDHVWSYDFMVDRTANGRAFKTLNIIDEYTRVCLKIKVNRKIKSQDVIDQLYDLFIFRGIPEYIRSDNGPE
FTAKAVRKWLSRLGVKTLFIEPGSPWENGYIESFNGKLRDELLDREIFTTLEEAKVLIEQWRREYNQVRPHSSLGYRPPAPEAVLTMMTS
EGLKVPQKQPKRGRLWLNDGSCIRLRPEYKDHVWSYDFMVDRTANGRAFKTLNIIDEYTRVCLKIKVNRKIKSQDVIDQLYDLFIFRGIPEYIRSDNGPE
FTAKAVRKWLSRLGVKTLFIEPGSPWENGYIESFNGKLRDELLDREIFTTLEEAKVLIEQWRREYNQVRPHSSLGYRPPAPEAVLTMMTS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1115 bp | 371 aa | 67 | 1181 | + | Yes |
Chemistry : DDE
ORF sequence :
MPRKAFTAEQIINKLREAELILNQGTTVPEACRKIRVTDQTYYRWCKEYGGLNIDQAKRLKDLEKENVQLKRLVADLSLDKAILKEASRGKLLSPSQRRQ
VIAKVRISLGISERRACQVLGQARSTQRRKESITDDEAKLTLRIIELASKYGRYGYRRITAILQIAGWRVNHKRVERIWRKEGLKVPQKQPKRGRLWLND
GSCIRLRPEYKDHVWSYDFMVDRTANGRAFKTLNIIDEYTRVCLKIKVNRKIKSQDVIDQLYDLFIFRGIPEYIRSDNGPEFTAKAVRKWLSRLGVKTLF
IEPGSPWENGYIESFNGKLRDELLDREIFTTLEEAKVLIEQWRREYNQVRPHSSLGYRPPAPEAVLTMMTS
VIAKVRISLGISERRACQVLGQARSTQRRKESITDDEAKLTLRIIELASKYGRYGYRRITAILQIAGWRVNHKRVERIWRKEGLKVPQKQPKRGRLWLND
GSCIRLRPEYKDHVWSYDFMVDRTANGRAFKTLNIIDEYTRVCLKIKVNRKIKSQDVIDQLYDLFIFRGIPEYIRSDNGPEFTAKAVRKWLSRLGVKTLF
IEPGSPWENGYIESFNGKLRDELLDREIFTTLEEAKVLIEQWRREYNQVRPHSSLGYRPPAPEAVLTMMTS
Blast result :
Comments
ISDsp2 is 68 % (ORFA) aa similar to ISMyma1 and 61% (ORFB) aa similar to ISBp1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Kube,M., Beck,A., Zinder,S.H., Kuhl,H., Reinhardt,R. and Adrian,L. (2005) (er) Nat. Biotechnol. In press.