ISBlma5
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AANZ01000041 | ND | Blastopirellula marina | Blastopirellula marina DSM 3645 |
DNA section
IS Length : 1296 bp
Ends
IR Length : 28/42
IRL : TGGAATGCTCCCCAAATCCTGATCCAGATGTTATGAAAGTCTAGAGCCAA
IRR : TGGCCTGCCCCAATTTTCTGTACCAAGCGTTATGAAAGATTGGTTTGGGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAACGGCTTC | TGCTGGAGCG | 0 |
DNA sequence
TGGAATGCTCCCCAAATCCTGATCCAGATGTTATGAAAGTCTAGAGCCAATTTCGGCGAAGGATTTTTCATGAGCAAGAAGCTCCGACGGCATTCGGCCG
AACAGATCATCAAGAAGTTGCGGAACGCGGACGCCATGGTGGCGGCTGGCAAGAGCGTCGGAGAAGTGCTGCAGGCGCTGGAAGTCAGCGAGGCCACTTT
GAGCCGCTGGCGATCGCAGTATGGCGGGATGAAGAGCGAAGAGGCGAAGCGTCTCAAGTCGCTCGAAGAAGAGAACAATCGACTGAAAAAGATCGTCGCC
GATCAGGCCTTGGATATCTCGATGCTGAAGGAGATCGCCAAGGGAAACTGACCACCCCTCAGTCGCGACGCGCGGCGGTCAAGCAGCTTCAGGAGAAGTA
TTCAGTCTCCGAAAGGCGGGCCTGTCGCGTGCTCGATCAGCCTCGATCGAGCCAACGATTTGAGGGGAAGCCGAAGACGAAGACGTGCGTCTGACCAAGC
GGATTTTAGAGCTTGTACGGCAGCGACCGCGCTGGGGTTATCGTCAGATCTGTCAACTGTTGCGGCGCGAGGGGGAGACCTTGAACATGAAAAAGATGTA
CCGACTTTGGAAAGCGGCCGGGCTGAAAGTTCCGCAAAAACGCCGTAAAAAGCGGGCGACAGGCGTCTCGACGAACGCTTGCCACGTGCAACCGGCCGGG
TTCAGGCACGACGTGTGGACATGGGATTTCATTCAGTCATCGACGATCGATGGCAGGACGATCCGCTTTTTGAACATCGTTGATGAGTACACGCGGCAGT
GCCTGGCGATCAAGGTTGGCCGCAGCATTACCAGCGAAGACGCGATCGATACGCTCGCCGAGCTGTTCGCGATGCATGGCGTGCCGAAGCGGATTCGCTG
CGACAACGGGCCAGAGTTTATCTCCTGCGCGATCAAGACCTGGCTGGACCTGATCGGCGTCGAAGTACTCTACATCGAACCAGGATCGCCCTGGCAAAAC
GGCTTGTGCGTAAGCTTCAACAGCCGCCTTCGCGACGAATACCTGCACCAGACTGACCTGCTGAGCTTGGAGGACGCAAGGATCAAGGCCCGGGCCTGGC
GCGAGGACTTCAACCACAACCGCCCGCACAGCTCGCTCGGCTACCTGACCCCGGCCGAGTTCGCGCGCCGCTGTGCTGCTTCCACTTCAGTCGCTGCGCT
CCTTCCGTTCCAGCAGCACAGCGACTCCATTGAATCGTTACCTGTTTCCCAAACCAATCTTTCATAACGCTTGGTACAGAAAATTGGGGCAGGCCA
AACAGATCATCAAGAAGTTGCGGAACGCGGACGCCATGGTGGCGGCTGGCAAGAGCGTCGGAGAAGTGCTGCAGGCGCTGGAAGTCAGCGAGGCCACTTT
GAGCCGCTGGCGATCGCAGTATGGCGGGATGAAGAGCGAAGAGGCGAAGCGTCTCAAGTCGCTCGAAGAAGAGAACAATCGACTGAAAAAGATCGTCGCC
GATCAGGCCTTGGATATCTCGATGCTGAAGGAGATCGCCAAGGGAAACTGACCACCCCTCAGTCGCGACGCGCGGCGGTCAAGCAGCTTCAGGAGAAGTA
TTCAGTCTCCGAAAGGCGGGCCTGTCGCGTGCTCGATCAGCCTCGATCGAGCCAACGATTTGAGGGGAAGCCGAAGACGAAGACGTGCGTCTGACCAAGC
GGATTTTAGAGCTTGTACGGCAGCGACCGCGCTGGGGTTATCGTCAGATCTGTCAACTGTTGCGGCGCGAGGGGGAGACCTTGAACATGAAAAAGATGTA
CCGACTTTGGAAAGCGGCCGGGCTGAAAGTTCCGCAAAAACGCCGTAAAAAGCGGGCGACAGGCGTCTCGACGAACGCTTGCCACGTGCAACCGGCCGGG
TTCAGGCACGACGTGTGGACATGGGATTTCATTCAGTCATCGACGATCGATGGCAGGACGATCCGCTTTTTGAACATCGTTGATGAGTACACGCGGCAGT
GCCTGGCGATCAAGGTTGGCCGCAGCATTACCAGCGAAGACGCGATCGATACGCTCGCCGAGCTGTTCGCGATGCATGGCGTGCCGAAGCGGATTCGCTG
CGACAACGGGCCAGAGTTTATCTCCTGCGCGATCAAGACCTGGCTGGACCTGATCGGCGTCGAAGTACTCTACATCGAACCAGGATCGCCCTGGCAAAAC
GGCTTGTGCGTAAGCTTCAACAGCCGCCTTCGCGACGAATACCTGCACCAGACTGACCTGCTGAGCTTGGAGGACGCAAGGATCAAGGCCCGGGCCTGGC
GCGAGGACTTCAACCACAACCGCCCGCACAGCTCGCTCGGCTACCTGACCCCGGCCGAGTTCGCGCGCCGCTGTGCTGCTTCCACTTCAGTCGCTGCGCT
CCTTCCGTTCCAGCAGCACAGCGACTCCATTGAATCGTTACCTGTTTCCCAAACCAATCTTTCATAACGCTTGGTACAGAAAATTGGGGCAGGCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 70 | 351 | + | No |
Description : First part of the transposase
ORF sequence :
MSKKLRRHSAEQIIKKLRNADAMVAAGKSVGEVLQALEVSEATLSRWRSQYGGMKSEEAKRLKSLEEENNRLKKIVADQALDISMLKEIAKGN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 939 bp | 312 aa | 329 | 1267 | + | No |
Description : Second part of the transposase
ORF sequence :
RRSPRETDHPSVATRGGQAASGEVFSLRKAGLSRARSASIEPTI*GEAEDEDVRLTKRILELVRQRPRWGYRQICQLLRREGETLNMKKMYRLWKAAGLK
VPQKRRKKRATGVSTNACHVQPAGFRHDVWTWDFIQSSTIDGRTIRFLNIVDEYTRQCLAIKVGRSITSEDAIDTLAELFAMHGVPKRIRCDNGPEFISC
AIKTWLDLIGVEVLYIEPGSPWQNGLCVSFNSRLRDEYLHQTDLLSLEDARIKARAWREDFNHNRPHSSLGYLTPAEFARRCAASTSVAALLPFQQHSDS
IESLPVSQTNLS
VPQKRRKKRATGVSTNACHVQPAGFRHDVWTWDFIQSSTIDGRTIRFLNIVDEYTRQCLAIKVGRSITSEDAIDTLAELFAMHGVPKRIRCDNGPEFISC
AIKTWLDLIGVEVLYIEPGSPWQNGLCVSFNSRLRDEYLHQTDLLSLEDARIKARAWREDFNHNRPHSSLGYLTPAEFARRCAASTSVAALLPFQQHSDS
IESLPVSQTNLS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1198 bp | 399 aa | 70 | 1267 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKKLRRHSAEQIIKKLRNADAMVAAGKSVGEVLQALEVSEATLSRWRSQYGGMKSEEAKRLKSLEEENNRLKKIVADQALDISMLKEIAKGETDHPSVA
TRGGQAASGEVFSLRKAGLSRARSASIEPTI*GEAEDEDVRLTKRILELVRQRPRWGYRQICQLLRREGETLNMKKMYRLWKAAGLKVPQKRRKKRATGV
STNACHVQPAGFRHDVWTWDFIQSSTIDGRTIRFLNIVDEYTRQCLAIKVGRSITSEDAIDTLAELFAMHGVPKRIRCDNGPEFISCAIKTWLDLIGVEV
LYIEPGSPWQNGLCVSFNSRLRDEYLHQTDLLSLEDARIKARAWREDFNHNRPHSSLGYLTPAEFARRCAASTSVAALLPFQQHSDSIESLPVSQTNLS
TRGGQAASGEVFSLRKAGLSRARSASIEPTI*GEAEDEDVRLTKRILELVRQRPRWGYRQICQLLRREGETLNMKKMYRLWKAAGLKVPQKRRKKRATGV
STNACHVQPAGFRHDVWTWDFIQSSTIDGRTIRFLNIVDEYTRQCLAIKVGRSITSEDAIDTLAELFAMHGVPKRIRCDNGPEFISCAIKTWLDLIGVEV
LYIEPGSPWQNGLCVSFNSRLRDEYLHQTDLLSLEDARIKARAWREDFNHNRPHSSLGYLTPAEFARRCAASTSVAALLPFQQHSDSIESLPVSQTNLS
Blast result :
Comments
Genome in project.
ISBlma5 is 73 % (ORFA) aa similar to ISMyma1 and 66% (ORFB) aa similar to ISNha4. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ORFB has an interrupted stop codon at position 461.
ISBlma5 is 73 % (ORFA) aa similar to ISMyma1 and 66% (ORFB) aa similar to ISNha4. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ORFB has an interrupted stop codon at position 461.
References
1] Amann,R., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.