ISIba1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAMX01000015 | ND | Idiomarina baltica | Idiomarina baltica OS145 |
DNA section
IS Length : 1197 bp
Ends
IR Length : 36/49
IRL : TGACGTCTCCCCCAAAAATAGGACCATGACATCGATGAGATTTCCATTAA
IRR : TGACCTTTCCCCTGAACTAACACCATCTTATCGATGAAATTTCAGTTTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGTGTCAGT | CCCCTCACAG | 0 |
DNA sequence
TGACGTCTCCCCCAAAAATAGGACCATGACATCGATGAGATTTCCATTAAAATGGGTCAAGTGGAGATCTCGATATGAAAAAACGTTACAGCGAAGAACA
AATAATCAAAGCCATCAAAGAGCATGAGGCCGGTGCAAAAGTTGACGAAATTTGTCGTCGCATGGGCATTTCCAGCGGCACGTTTTATAACTGGCGTAGT
AAATACGCCGGAATGGAAGTGAATGAGGCCAAGCGTTTAAAAGAGCTTGAGTCTGAGAATAGCAAGCTTAAAAAGCTACTGGCGGAGAAAATTCTTGAGA
CAGAAGCCTTAAAGGACGTTGTCTCAAAAAAGTGGTGAAGCCCGCCAGCAAGAAGCGTGTCGTAGAGCACTTAATCTCTGCGTATAAGCTAAGCGAGCGT
CTTGCGTGCAAGTTGGTCGGGCTGAGCCGAACAGCGTACCGATATAAGCCAAAGCAACGAGATGACGAGCGGGTAACAACTCGCCTAAAGGAGCTTGCCG
TGGAATATCCACGGTATGGCTATTTACTGCTGCATGGGCTGCTTAAGCGCGAGGGGCTTGTAAAGAATAAGAAGCGAACCTATCGCCTTTATACCGAACA
AGGGCTTCAGGTTCGGACCAAGAAACGTAAAAAGCTGAGACGACCGCGTGTCGTTATGGATGCACCGCAGCGTGTTAATAAGCGATGGTCGATGGACTTT
GTGTCTGACCAGCTGGCAAGTGGCCGACGCTTCAGAGTCTTGAACGTTGTCGATGACTTTAGCCGGGAAATGGTTGGGCAACTTGTCTCAGTATCGATTT
CAGGACAGCAAGTATCGCGCTTTTTGTCTCAGCTTATCGAGGAGCGCAGAAAGCCAGAGCATATTGTCTGCGATAATGGCACCGAATTTACCAGTAAAGC
CATGTTTTTCTGGAGCAAAAGCAACGATGTAAGGTTGAGTTTTATTCAGCCAGGAAAACCAACACAGAACGCTTTTGTTGAGAGTTTGAATGGTAAGTTT
AGGAATGAATGTCTGGATCAGAACTGGTTTAGAACGCTCGACGAAGCGAGGTATGAAATAGAGCAATGGCAGCGTCATTACAATTACGAACGTCCGCATA
GCTCGTTAAATTATCTACCGCCTGTGGAGTTTGCAAAACAGGTAGCTTAAAACTGAAATTTCATCGATAAGATGGTGTTAGTTCAGGGGAAAGGTCA
AATAATCAAAGCCATCAAAGAGCATGAGGCCGGTGCAAAAGTTGACGAAATTTGTCGTCGCATGGGCATTTCCAGCGGCACGTTTTATAACTGGCGTAGT
AAATACGCCGGAATGGAAGTGAATGAGGCCAAGCGTTTAAAAGAGCTTGAGTCTGAGAATAGCAAGCTTAAAAAGCTACTGGCGGAGAAAATTCTTGAGA
CAGAAGCCTTAAAGGACGTTGTCTCAAAAAAGTGGTGAAGCCCGCCAGCAAGAAGCGTGTCGTAGAGCACTTAATCTCTGCGTATAAGCTAAGCGAGCGT
CTTGCGTGCAAGTTGGTCGGGCTGAGCCGAACAGCGTACCGATATAAGCCAAAGCAACGAGATGACGAGCGGGTAACAACTCGCCTAAAGGAGCTTGCCG
TGGAATATCCACGGTATGGCTATTTACTGCTGCATGGGCTGCTTAAGCGCGAGGGGCTTGTAAAGAATAAGAAGCGAACCTATCGCCTTTATACCGAACA
AGGGCTTCAGGTTCGGACCAAGAAACGTAAAAAGCTGAGACGACCGCGTGTCGTTATGGATGCACCGCAGCGTGTTAATAAGCGATGGTCGATGGACTTT
GTGTCTGACCAGCTGGCAAGTGGCCGACGCTTCAGAGTCTTGAACGTTGTCGATGACTTTAGCCGGGAAATGGTTGGGCAACTTGTCTCAGTATCGATTT
CAGGACAGCAAGTATCGCGCTTTTTGTCTCAGCTTATCGAGGAGCGCAGAAAGCCAGAGCATATTGTCTGCGATAATGGCACCGAATTTACCAGTAAAGC
CATGTTTTTCTGGAGCAAAAGCAACGATGTAAGGTTGAGTTTTATTCAGCCAGGAAAACCAACACAGAACGCTTTTGTTGAGAGTTTGAATGGTAAGTTT
AGGAATGAATGTCTGGATCAGAACTGGTTTAGAACGCTCGACGAAGCGAGGTATGAAATAGAGCAATGGCAGCGTCATTACAATTACGAACGTCCGCATA
GCTCGTTAAATTATCTACCGCCTGTGGAGTTTGCAAAACAGGTAGCTTAAAACTGAAATTTCATCGATAAGATGGTGTTAGTTCAGGGGAAAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 87 aa | 75 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRYSEEQIIKAIKEHEAGAKVDEICRRMGISSGTFYNWRSKYAGMEVNEAKRLKELESENSKLKKLLAEKILETEALKDVVSKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 852 bp | 283 aa | 299 | 1150 | + | No |
Description : Second part of the transposase
ORF sequence :
DRSLKGRCLKKVVKPASKKRVVEHLISAYKLSERLACKLVGLSRTAYRYKPKQRDDERVTTRLKELAVEYPRYGYLLLHGLLKREGLVKNKKRTYRLYTE
QGLQVRTKKRKKLRRPRVVMDAPQRVNKRWSMDFVSDQLASGRRFRVLNVVDDFSREMVGQLVSVSISGQQVSRFLSQLIEERRKPEHIVCDNGTEFTSK
AMFFWSKSNDVRLSFIQPGKPTQNAFVESLNGKFRNECLDQNWFRTLDEARYEIEQWQRHYNYERPHSSLNYLPPVEFAKQVA
QGLQVRTKKRKKLRRPRVVMDAPQRVNKRWSMDFVSDQLASGRRFRVLNVVDDFSREMVGQLVSVSISGQQVSRFLSQLIEERRKPEHIVCDNGTEFTSK
AMFFWSKSNDVRLSFIQPGKPTQNAFVESLNGKFRNECLDQNWFRTLDEARYEIEQWQRHYNYERPHSSLNYLPPVEFAKQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1076 bp | 358 aa | 75 | 1150 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRYSEEQIIKAIKEHEAGAKVDEICRRMGISSGTFYNWRSKYAGMEVNEAKRLKELESENSKLKKLLAEKILETEALKDVVSKKVVKPASKKRVVEHL
ISAYKLSERLACKLVGLSRTAYRYKPKQRDDERVTTRLKELAVEYPRYGYLLLHGLLKREGLVKNKKRTYRLYTEQGLQVRTKKRKKLRRPRVVMDAPQR
VNKRWSMDFVSDQLASGRRFRVLNVVDDFSREMVGQLVSVSISGQQVSRFLSQLIEERRKPEHIVCDNGTEFTSKAMFFWSKSNDVRLSFIQPGKPTQNA
FVESLNGKFRNECLDQNWFRTLDEARYEIEQWQRHYNYERPHSSLNYLPPVEFAKQVA
ISAYKLSERLACKLVGLSRTAYRYKPKQRDDERVTTRLKELAVEYPRYGYLLLHGLLKREGLVKNKKRTYRLYTEQGLQVRTKKRKKLRRPRVVMDAPQR
VNKRWSMDFVSDQLASGRRFRVLNVVDDFSREMVGQLVSVSISGQQVSRFLSQLIEERRKPEHIVCDNGTEFTSKAMFFWSKSNDVRLSFIQPGKPTQNA
FVESLNGKFRNECLDQNWFRTLDEARYEIEQWQRHYNYERPHSSLNYLPPVEFAKQVA
Blast result :
Comments
Genome in progress.
ISIba1 is 83 % (ORFA) aa similar to ISOba1 and 64% (ORFB) aa similar to ISRpa2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISIba1 is 83 % (ORFA) aa similar to ISOba1 and 64% (ORFB) aa similar to ISRpa2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Brettar,I., Hofle,M., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.