ISYpe1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003131 | ND | Yersinia pestis | Yersinia pestis CO92 |
DNA section
IS Length : 1212 bp
Ends
IR Length : 16/39
IRL : TGATCTTCCCCCTTAGTTCAGTACCGCTGTAAAGTAGAGTTTTCGGCCCT
IRR : TGATCCCTCCCCAATATCCGTACCAGGCTAAATCAGAGATCCGGACCTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGGATTGCT | AATTTGCCTG | 0 |
DNA sequence
TGATCTTCCCCCTTAGTTCAGTACCGCTGTAAAGTAGAGTTTTCGGCCCTTCTTTTCCGTCCAAAAAGGGGTCATTCGTTATGAAAAAGACGCGTTATAC
CGAAGAACAAATCGCTTTTGCACTCAAACAGGCTGAAACGGGTACTCGAGTGGAAGAAGTCTGTAGGAAGATGGGCATTTCGGAAGCGACATTTTACAAC
TGGAAGAAGAAGTTTGGGGGAATGGGTGTCACGGAATTGCGCCGTCTTCGGCAGCTTGAGGATGAGAATCAGCGCCTCAAACGTCTGGTGGCCGATCTGA
GTCTGGATAAGGAAATGCTGCAGGATGTCATCCGAAAAAAGTTCTGAGGCCGCTGCAGAAGCGTGATGCCGTGGAATATCTGCTGGCGGCGTACCGTATC
GGAGTACGCAGAGGGTGCAGGCTGATGATGCAGAGCCGCACTGTATACAACTATCGTAGCCATCGTGACGATCGGGCTATTACTCAGCGAATACGGGAAA
TTGCGGAGACGCGCATCCGTTATGGCTGTCCGCGCATTCATATCCTGCTGCGGAGAGAAGGCTGGCTTGTTAACCATAAGAAAACGCACCGTATTTACTG
TCTTGAGGGTCTCAATCTACGTTCGAAACGTCCACGGCGGCATGTGACAGCGAAGCACAGGCACGCACGTCCAGAAGTGACCGCGTTAGAGCAGTGCTGG
AGCATGGATTTCGTTGCTGATAATCTGTTCAACGGGCGTCGGGTCAGGGCGCTAACTATAGTGGATAATTTTAGTCGTGAATGTCTGGCGATCGAGGTCG
GTCAGGGGTTACGTGGAGATGATGTTGTGGCTGTCATGGACAGATTAAAACATTCGCTGGGGCGTATTCCACAAAGGCTGCAGACAGATAACGGCAGCGA
ATTCATCTCGAAGTCGATGGACCGATGGGCGTATGAAAACAGGGTCACGATGGACTGTTCACGCCCCGGAAAGCCTACAGATAATGCCTTTATCGAGTCA
TTTAATGGCAGTCTGAGGGATGAATGTCTGAACGTGCACGGGTTCCTTTCTCTGGAAGATGCTCAGGAGAAAATTGAACAATGGCGGCAAGAATATAATC
ATTTTCATCCGCATTCTTCGTTAAATAACCTGACTCCGGCAGAATTTGCCCGAAGTCATCAAAAAGGTCCGGATCTCTGATTTAGCCTGGTACGGATATT
GGGGAGGGATCA
CGAAGAACAAATCGCTTTTGCACTCAAACAGGCTGAAACGGGTACTCGAGTGGAAGAAGTCTGTAGGAAGATGGGCATTTCGGAAGCGACATTTTACAAC
TGGAAGAAGAAGTTTGGGGGAATGGGTGTCACGGAATTGCGCCGTCTTCGGCAGCTTGAGGATGAGAATCAGCGCCTCAAACGTCTGGTGGCCGATCTGA
GTCTGGATAAGGAAATGCTGCAGGATGTCATCCGAAAAAAGTTCTGAGGCCGCTGCAGAAGCGTGATGCCGTGGAATATCTGCTGGCGGCGTACCGTATC
GGAGTACGCAGAGGGTGCAGGCTGATGATGCAGAGCCGCACTGTATACAACTATCGTAGCCATCGTGACGATCGGGCTATTACTCAGCGAATACGGGAAA
TTGCGGAGACGCGCATCCGTTATGGCTGTCCGCGCATTCATATCCTGCTGCGGAGAGAAGGCTGGCTTGTTAACCATAAGAAAACGCACCGTATTTACTG
TCTTGAGGGTCTCAATCTACGTTCGAAACGTCCACGGCGGCATGTGACAGCGAAGCACAGGCACGCACGTCCAGAAGTGACCGCGTTAGAGCAGTGCTGG
AGCATGGATTTCGTTGCTGATAATCTGTTCAACGGGCGTCGGGTCAGGGCGCTAACTATAGTGGATAATTTTAGTCGTGAATGTCTGGCGATCGAGGTCG
GTCAGGGGTTACGTGGAGATGATGTTGTGGCTGTCATGGACAGATTAAAACATTCGCTGGGGCGTATTCCACAAAGGCTGCAGACAGATAACGGCAGCGA
ATTCATCTCGAAGTCGATGGACCGATGGGCGTATGAAAACAGGGTCACGATGGACTGTTCACGCCCCGGAAAGCCTACAGATAATGCCTTTATCGAGTCA
TTTAATGGCAGTCTGAGGGATGAATGTCTGAACGTGCACGGGTTCCTTTCTCTGGAAGATGCTCAGGAGAAAATTGAACAATGGCGGCAAGAATATAATC
ATTTTCATCCGCATTCTTCGTTAAATAACCTGACTCCGGCAGAATTTGCCCGAAGTCATCAAAAAGGTCCGGATCTCTGATTTAGCCTGGTACGGATATT
GGGGAGGGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 81 | 347 | + | No |
Description : First part of the transposase
ORF sequence :
MKKTRYTEEQIAFALKQAETGTRVEEVCRKMGISEATFYNWKKKFGGMGVTELRRLRQLEDENQRLKRLVADLSLDKEMLQDVIRKKF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 870 bp | 289 aa | 311 | 1180 | + | No |
Description : Second part of the transposase
ORF sequence :
GNAAGCHPKKVLRPLQKRDAVEYLLAAYRIGVRRGCRLMMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEG
LNLRSKRPRRHVTAKHRHARPEVTALEQCWSMDFVADNLFNGRRVRALTIVDNFSRECLAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFIS
KSMDRWAYENRVTMDCSRPGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFHPHSSLNNLTPAEFARSHQKGPDL
LNLRSKRPRRHVTAKHRHARPEVTALEQCWSMDFVADNLFNGRRVRALTIVDNFSRECLAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFIS
KSMDRWAYENRVTMDCSRPGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFHPHSSLNNLTPAEFARSHQKGPDL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1100 bp | 366 aa | 81 | 1180 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKTRYTEEQIAFALKQAETGTRVEEVCRKMGISEATFYNWKKKFGGMGVTELRRLRQLEDENQRLKRLVADLSLDKEMLQDVIRKKVLRPLQKRDAVEY
LLAAYRIGVRRGCRLMMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEGLNLRSKRPRRHVTAKHRHARPEV
TALEQCWSMDFVADNLFNGRRVRALTIVDNFSRECLAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFISKSMDRWAYENRVTMDCSRPGKPT
DNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFHPHSSLNNLTPAEFARSHQKGPDL
LLAAYRIGVRRGCRLMMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEGLNLRSKRPRRHVTAKHRHARPEV
TALEQCWSMDFVADNLFNGRRVRALTIVDNFSRECLAIEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFISKSMDRWAYENRVTMDCSRPGKPT
DNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFHPHSSLNNLTPAEFARSHQKGPDL
Blast result :
Comments
ISYpe1 is 93% (ORFA) and 73% (ORFB) aa similar to ISRso12. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Parkhill,J., Wren,B.W., Thomson,N.R., Titball,R.W., Holden,M.T., Prentice,M.B., Sebaihia,M., James,K.D., Churcher,C., Mungall,K.L., Baker,S., Basham,D., Bentley,S.D., Brooks,K., Cerdeno-Tarraga,A.M., Chillingworth,T., Cronin,A., Davies,R.M., Davis,P., Dougan,G., Feltwell,T., Hamlin,N., Holroyd,S., Jagels,K., Karlyshev,A.V., Leather,S., Moule,S., Oyston,P.C., Quail,M., Rutherford,K., Simmonds,M., Skelton,J., Stevens,K., Whitehead,S. and Barrell,B.G. (2001) Nature 413 (6855), 523-527.