ISLbl2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AANC01000001 | ND | Leeuwenhoekiella blandensis | Leeuwenhoekiella blandensis MED217 |
DNA section
IS Length : 1216 bp
Ends
IR Length : 22/30
IRL : TGTAAGCTTCCCTTCAAACCGAACTGTTTAGAAGTGTAATAATTCTTAAT
IRR : TGTAAGCTTCCCCAGACTAGAACTGCTTAATCTTCCAAAACTGGTTTAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCGTTAGAG | GTTA | GTGTATGTCC | 4 |
DNA sequence
TGTAAGCTTCCCTTCAAACCGAACTGTTTAGAAGTGTAATAATTCTTAATTTTAAACAGTATGAGACGAAGCAAATTCACACCTACACAGATTGCAAAAA
TTTTAAAGGAGTTCGACAACGGTAAGAGTGTTGAAGAAATTAGCCGCGAATACGGTGTCAGTACCTCTACCTTTTATAAATGGCGTTCTAAATACGCTGG
TATGAGCAGCAAAGAACTTAAGCGTATTAAGGAGCTCGAAGCTGAAAACGCCCGTCTAAAGCAGATGTATGCCTCTTTAGCACTAGACCACCAAATGGCA
AAAGAGATTATTGAAAAAAAGCTCTAAAGCCCTGCCGTAAACGCACCATAGCTAAAGAACTTATCCGCTACGGCATCAGTAGGGCGTGCCGAGTGCTTAA
TATGAGTAAAAGTGTTTATTATTACAAACCTTTACCCAAGGATGATACTCCTATAGAACAAGCTCTTAGAGACAAAGCAGAAGAACATTCAGAAGAGGGT
TTCTGGAAAGCCTACTGGCGTATCCGTAACGAAGGAAAGCCTTGGAATCATAAGCGGGTACACAGGGTGTATGTATCTTTAGGGCTTCCTTTAAGAAGAA
AAGCAAAGAAGCGCCTGCCCGCGCGTATACAGGAGCCTTTGGAGATTCCTGAAGAATTGAATGATACTTGGAGTATTGATTTCGTAAGCGATGTCCTAGA
AAACAAGAGACGTTTTAGATCTTTTAACGTAATAGACGATTTTAATCGAGAGGCACTACATATTGAAGTAGACTTTTCTTTAACCAGTAATCGGGTAGTC
TGGGTACTTAATCACCTAATTAACCGACGAGGTAAGCCAAATAGGATCAGGATGGACAATGGTCCTGAATTCATAGCAAACCTAACTGCTGAATGGAGTA
AAATGCATGAAATTGAATTTAAATACATACAACCAGGCAAACCTACTCAAAATGCTTTTGTAGAACGCTTTAATGGCTCCTATCGCAGAGGTGTCCTAGA
TAAATATATCTTTGAAAACATTGATCAGGTAAGGGAACAAACTCAAATATGGATGGAAGATTACAATAAATATCGTCCCCATGACTCACTGGGCAAAATA
GCACCAGTGCAATACGCAAAACTCCATTCTCTTGGGGCTAGCCCCAAGAGAATGAAAAACAATAATTTTAAACCAGTTTTGGAAGATTAAGCAGTTCTAG
TCTGGGGAAGCTTACA
TTTTAAAGGAGTTCGACAACGGTAAGAGTGTTGAAGAAATTAGCCGCGAATACGGTGTCAGTACCTCTACCTTTTATAAATGGCGTTCTAAATACGCTGG
TATGAGCAGCAAAGAACTTAAGCGTATTAAGGAGCTCGAAGCTGAAAACGCCCGTCTAAAGCAGATGTATGCCTCTTTAGCACTAGACCACCAAATGGCA
AAAGAGATTATTGAAAAAAAGCTCTAAAGCCCTGCCGTAAACGCACCATAGCTAAAGAACTTATCCGCTACGGCATCAGTAGGGCGTGCCGAGTGCTTAA
TATGAGTAAAAGTGTTTATTATTACAAACCTTTACCCAAGGATGATACTCCTATAGAACAAGCTCTTAGAGACAAAGCAGAAGAACATTCAGAAGAGGGT
TTCTGGAAAGCCTACTGGCGTATCCGTAACGAAGGAAAGCCTTGGAATCATAAGCGGGTACACAGGGTGTATGTATCTTTAGGGCTTCCTTTAAGAAGAA
AAGCAAAGAAGCGCCTGCCCGCGCGTATACAGGAGCCTTTGGAGATTCCTGAAGAATTGAATGATACTTGGAGTATTGATTTCGTAAGCGATGTCCTAGA
AAACAAGAGACGTTTTAGATCTTTTAACGTAATAGACGATTTTAATCGAGAGGCACTACATATTGAAGTAGACTTTTCTTTAACCAGTAATCGGGTAGTC
TGGGTACTTAATCACCTAATTAACCGACGAGGTAAGCCAAATAGGATCAGGATGGACAATGGTCCTGAATTCATAGCAAACCTAACTGCTGAATGGAGTA
AAATGCATGAAATTGAATTTAAATACATACAACCAGGCAAACCTACTCAAAATGCTTTTGTAGAACGCTTTAATGGCTCCTATCGCAGAGGTGTCCTAGA
TAAATATATCTTTGAAAACATTGATCAGGTAAGGGAACAAACTCAAATATGGATGGAAGATTACAATAAATATCGTCCCCATGACTCACTGGGCAAAATA
GCACCAGTGCAATACGCAAAACTCCATTCTCTTGGGGCTAGCCCCAAGAGAATGAAAAACAATAATTTTAAACCAGTTTTGGAAGATTAAGCAGTTCTAG
TCTGGGGAAGCTTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 61 | 327 | + | No |
Description : First part of the transposase
ORF sequence :
MRRSKFTPTQIAKILKEFDNGKSVEEISREYGVSTSTFYKWRSKYAGMSSKELKRIKELEAENARLKQMYASLALDHQMAKEIIEKKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 876 bp | 291 aa | 315 | 1190 | + | No |
Description : Second part of the transposase
ORF sequence :
KKALKPCRKRTIAKELIRYGISRACRVLNMSKSVYYYKPLPKDDTPIEQALRDKAEEHSEEGFWKAYWRIRNEGKPWNHKRVHRVYVSLGLPLRRKAKKR
LPARIQEPLEIPEELNDTWSIDFVSDVLENKRRFRSFNVIDDFNREALHIEVDFSLTSNRVVWVLNHLINRRGKPNRIRMDNGPEFIANLTAEWSKMHEI
EFKYIQPGKPTQNAFVERFNGSYRRGVLDKYIFENIDQVREQTQIWMEDYNKYRPHDSLGKIAPVQYAKLHSLGASPKRMKNNNFKPVLED
LPARIQEPLEIPEELNDTWSIDFVSDVLENKRRFRSFNVIDDFNREALHIEVDFSLTSNRVVWVLNHLINRRGKPNRIRMDNGPEFIANLTAEWSKMHEI
EFKYIQPGKPTQNAFVERFNGSYRRGVLDKYIFENIDQVREQTQIWMEDYNKYRPHDSLGKIAPVQYAKLHSLGASPKRMKNNNFKPVLED
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1130 bp | 376 aa | 61 | 1190 | + | Yes |
Chemistry : DDE
ORF sequence :
MRRSKFTPTQIAKILKEFDNGKSVEEISREYGVSTSTFYKWRSKYAGMSSKELKRIKELEAENARLKQMYASLALDHQMAKEIIEKKALKPCRKRTIAKE
LIRYGISRACRVLNMSKSVYYYKPLPKDDTPIEQALRDKAEEHSEEGFWKAYWRIRNEGKPWNHKRVHRVYVSLGLPLRRKAKKRLPARIQEPLEIPEEL
NDTWSIDFVSDVLENKRRFRSFNVIDDFNREALHIEVDFSLTSNRVVWVLNHLINRRGKPNRIRMDNGPEFIANLTAEWSKMHEIEFKYIQPGKPTQNAF
VERFNGSYRRGVLDKYIFENIDQVREQTQIWMEDYNKYRPHDSLGKIAPVQYAKLHSLGASPKRMKNNNFKPVLED
LIRYGISRACRVLNMSKSVYYYKPLPKDDTPIEQALRDKAEEHSEEGFWKAYWRIRNEGKPWNHKRVHRVYVSLGLPLRRKAKKRLPARIQEPLEIPEEL
NDTWSIDFVSDVLENKRRFRSFNVIDDFNREALHIEVDFSLTSNRVVWVLNHLINRRGKPNRIRMDNGPEFIANLTAEWSKMHEIEFKYIQPGKPTQNAF
VERFNGSYRRGVLDKYIFENIDQVREQTQIWMEDYNKYRPHDSLGKIAPVQYAKLHSLGASPKRMKNNNFKPVLED
Blast result :
Comments
Genome in progress.
ISLbl2 is 81% (ORFA) aa similar to ISD1 and 67% (ORFB) aa similar to IS1351.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISLbl2 is 81% (ORFA) aa similar to ISD1 and 67% (ORFB) aa similar to IS1351.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pinhassi,J., Pedros-Alio,C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.