ISSst1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAYA01000007 | ND | Sagittula stellata | Sagittula stellata E-37 |
DNA section
IS Length : 1167 bp
Ends
IR Length : 26/47
IRL : TGATCTGATCCCCGTATTCTGGGCCGTTTGAAGGTGGAGTTTTCCGCTAT
IRR : TGATGTCCCCTGATTTCGTCAGCGTGATCGGGGTCTTGTTGCCGATCGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCTCCTCGTT | CCAGCCTGTT | 0 |
DNA sequence
TGATCTGATCCCCGTATTCTGGGCCGTTTGAAGGTGGAGTTTTCCGCTATGATTTCCCGGCTGGGAGAGGAGCGGAATCAGATGAAGGCATCGAAGTTTT
CGGACGCGCAGAAGGCGTTCATCATCAAGCAGGGCGAAGAGGGCACGCCGGTTGCGGAGATCTGCCGCAAGGCGGGCATCAGCCAGGCCACCTACTTCAA
CTGGAAGAAGAAGTATGCGGGTCTGTTGCCGACGGAGATGAAGCGGTTGAAGCAGCTCGAGGATGAGAACGGCCGGCTGAAGAAGATCGTGGCCGATCTG
ACGCTGGACCGTGAGATGTTGCAGGATGTCATCCGGCGAAAGATCTGAAGCCTGGTCGGATGCGCGAACTGGTGCGCGGGATGTGCAGCGATTGGGCGGT
CTCGATCCGGACGGCGTGTGGCGCCATCGGATTTGATCGCTCGACGTTCCACTACAAATCCCGCCGCGTCGATCAGGCTGCCGTGGAAAAGCGGATCAAG
GAGATCTGCGAGACACGTGTGCGCTACGGCTATCGCCGGGTTCATGTGCTCTTGGACCGCGAGGGCTGGGGTATCAACATCAAGAAAGTCTATCGCATTT
ACAGGGAGTTGGGCATGCAGCTCAGGAACAAGACGCCGAAGCGGCGGGTGAAGGCCAAGCTGCGCGATGACCGTGCCGAAGCCGTCGGGCCGAACGATGT
CTGGGCGATGGATTTTGTCCATGACCAGCTGGCGACGGGCAGGAAGCTTCGGGTCTTGACGGTCGTGGACACGTTCTCGCGATACGTTCCGGTGCTCGAC
GCGCGGTTCAGCTATCGCGGCGAGGATGTCGTGGCGACACTGGACCGGGTGTGCCGCAGATCGGGCTATCCCAAGACGATCCGGGTCGATCAGGGCAGCG
AGTTCATCTCCCGCGACATGGACCTCTGGGCCTATCAGCGCGGTGTCACGCTCGACTTCTCACGGCCGGGAAAGCCAACAGACAATGCTTTCATTGAAGC
GTTCAATGGCCGGTTCCGTGCCGAGTGCCTGAACGCCCCTTGGTTCCTGACGCTTGCAGATGCGGCCGAAAAGTTGGAGGCTTGGCGTAGATACTAAAAC
GAGGAACGGCCACATGGCGCGATCGGCAACAAGACCCCGATCACGCTGACGAAATCAGGGGACATCA
CGGACGCGCAGAAGGCGTTCATCATCAAGCAGGGCGAAGAGGGCACGCCGGTTGCGGAGATCTGCCGCAAGGCGGGCATCAGCCAGGCCACCTACTTCAA
CTGGAAGAAGAAGTATGCGGGTCTGTTGCCGACGGAGATGAAGCGGTTGAAGCAGCTCGAGGATGAGAACGGCCGGCTGAAGAAGATCGTGGCCGATCTG
ACGCTGGACCGTGAGATGTTGCAGGATGTCATCCGGCGAAAGATCTGAAGCCTGGTCGGATGCGCGAACTGGTGCGCGGGATGTGCAGCGATTGGGCGGT
CTCGATCCGGACGGCGTGTGGCGCCATCGGATTTGATCGCTCGACGTTCCACTACAAATCCCGCCGCGTCGATCAGGCTGCCGTGGAAAAGCGGATCAAG
GAGATCTGCGAGACACGTGTGCGCTACGGCTATCGCCGGGTTCATGTGCTCTTGGACCGCGAGGGCTGGGGTATCAACATCAAGAAAGTCTATCGCATTT
ACAGGGAGTTGGGCATGCAGCTCAGGAACAAGACGCCGAAGCGGCGGGTGAAGGCCAAGCTGCGCGATGACCGTGCCGAAGCCGTCGGGCCGAACGATGT
CTGGGCGATGGATTTTGTCCATGACCAGCTGGCGACGGGCAGGAAGCTTCGGGTCTTGACGGTCGTGGACACGTTCTCGCGATACGTTCCGGTGCTCGAC
GCGCGGTTCAGCTATCGCGGCGAGGATGTCGTGGCGACACTGGACCGGGTGTGCCGCAGATCGGGCTATCCCAAGACGATCCGGGTCGATCAGGGCAGCG
AGTTCATCTCCCGCGACATGGACCTCTGGGCCTATCAGCGCGGTGTCACGCTCGACTTCTCACGGCCGGGAAAGCCAACAGACAATGCTTTCATTGAAGC
GTTCAATGGCCGGTTCCGTGCCGAGTGCCTGAACGCCCCTTGGTTCCTGACGCTTGCAGATGCGGCCGAAAAGTTGGAGGCTTGGCGTAGATACTAAAAC
GAGGAACGGCCACATGGCGCGATCGGCAACAAGACCCCGATCACGCTGACGAAATCAGGGGACATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 82 | 348 | + | No |
Description : First part of the transposase
ORF sequence :
MKASKFSDAQKAFIIKQGEEGTPVAEICRKAGISQATYFNWKKKYAGLLPTEMKRLKQLEDENGRLKKIVADLTLDREMLQDVIRRKI
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 783 bp | 260 aa | 315 | 1097 | + | No |
Description : Second part of the transposase
ORF sequence :
DVAGCHPAKDLKPGRMRELVRGMCSDWAVSIRTACGAIGFDRSTFHYKSRRVDQAAVEKRIKEICETRVRYGYRRVHVLLDREGWGINIKKVYRIYRELG
MQLRNKTPKRRVKAKLRDDRAEAVGPNDVWAMDFVHDQLATGRKLRVLTVVDTFSRYVPVLDARFSYRGEDVVATLDRVCRRSGYPKTIRVDQGSEFISR
DMDLWAYQRGVTLDFSRPGKPTDNAFIEAFNGRFRAECLNAPWFLTLADAAEKLEAWRRY
MQLRNKTPKRRVKAKLRDDRAEAVGPNDVWAMDFVHDQLATGRKLRVLTVVDTFSRYVPVLDARFSYRGEDVVATLDRVCRRSGYPKTIRVDQGSEFISR
DMDLWAYQRGVTLDFSRPGKPTDNAFIEAFNGRFRAECLNAPWFLTLADAAEKLEAWRRY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1016 bp | 338 aa | 82 | 1097 | + | Yes |
Chemistry : DDE
ORF sequence :
MKASKFSDAQKAFIIKQGEEGTPVAEICRKAGISQATYFNWKKKYAGLLPTEMKRLKQLEDENGRLKKIVADLTLDREMLQDVIRRKDLKPGRMRELVRG
MCSDWAVSIRTACGAIGFDRSTFHYKSRRVDQAAVEKRIKEICETRVRYGYRRVHVLLDREGWGINIKKVYRIYRELGMQLRNKTPKRRVKAKLRDDRAE
AVGPNDVWAMDFVHDQLATGRKLRVLTVVDTFSRYVPVLDARFSYRGEDVVATLDRVCRRSGYPKTIRVDQGSEFISRDMDLWAYQRGVTLDFSRPGKPT
DNAFIEAFNGRFRAECLNAPWFLTLADAAEKLEAWRRY
MCSDWAVSIRTACGAIGFDRSTFHYKSRRVDQAAVEKRIKEICETRVRYGYRRVHVLLDREGWGINIKKVYRIYRELGMQLRNKTPKRRVKAKLRDDRAE
AVGPNDVWAMDFVHDQLATGRKLRVLTVVDTFSRYVPVLDARFSYRGEDVVATLDRVCRRSGYPKTIRVDQGSEFISRDMDLWAYQRGVTLDFSRPGKPT
DNAFIEAFNGRFRAECLNAPWFLTLADAAEKLEAWRRY
Blast result :
Comments
Genome in progress.
ISSst1 is 95% (ORFA) and 77% (ORFB) aa similar to ISBrsp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSst1 is 95% (ORFA) and 77% (ORFB) aa similar to ISBrsp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Moran,M.A., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2006) Direct submission Genbank.