ISRosp2

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Roseobacter sp.
Roseobacter sp. MED193
DNA section
IS Length : 1254 bp

Ends


IR Length : 36/44

IRL : TGACCTGCCCCCCGAAACCTCCCGCGTTCTGATGTAGAGTTTGCTCAACC
IRR : TGACCTGCCCCCCGAGGCTCCCGCGTTCATAACGAGAGTTTGCGGGTTCG

Insertion site


Left flankDirect repeatRight flankDR Length
ACCACACCTGTCTATGGGCTAC0

DNA sequence

TGACCTGCCCCCCGAAACCTCCCGCGTTCTGATGTAGAGTTTGCTCAACCTTTCGAAGGAGCAAACAGATGCGAAAGAGCCGTTTCACGGAAGCCCAGAT
TATTGGGATGATCAAAGAACAAGAAGCCGGAATGCCGACGGCGGAGGTGTGTCGCAAGCATGGGCTCAGTCAGGGCACGTTCTACAAGTTCAAGTCGAAG
TATGGCGGTATGGAAGTGTCGGATGCGGCCAGGCTGAAGGCGCTGGAGGATGAGAATGCCAAGCTCAAGCGCCTGCTGGCTGATACAATGCTGGACAACG
TTGTGCTGAAGGACTTGCTGGGAAAGAGCTGACGACGCCGACCAAGCGACGAGCGGCAGCGCTCCGTGCAATGCGGGAACACGACATCTCGCAGCGCCGG
GTCTGCCGTCTTGTTGGTGTCGATCCCAAAACTGTCAGGCGCGACCGTCCGCCGGACAATCCCGAGATCCGCAAGGAGATGAAGGCTATTGCGAACACGC
GCCGCCGATTTGGCTACCGGCGGATCGGCGTGATGTTGGAACGCAAGGGAATGATCATGAATCACAAGAAGCTGTATCGACTCTATACGGAGGAAAAGCT
TGGCGTCAGGCGACGACGGGGCCGCAAACGGGCCCGTGGGTCACGAACACCGATGCCTGAGGCCCTCAAGCCTGGTGAGCGCTGGTCGCTGGACTTCTTG
TCCGACACGTTCGGGGCATCGCGCAGGTTCCGCATTCTGGCTGTGAACGATGATTGCTGCCGTGAGAACCTGTGCCTGATGGCCGACACCGGCATCTCAG
GGGCGCGCGTCGCCCGGGAGTTAGATGCCCTTGTCCGCATCTATGGGAAACCCGCCAGCATTGTCAGTGACAATGGGACGGAGTTCACCAGTCGGGCGAT
CCTGAAATGGGCCAACGACAATGGCGTGGACTGGCATTACATCGACCCGGGCAAGCCGCAGCAGAATGCCTTTATCGAAAGCTTCAATGGCAGTTTGCGC
GACGAGCTGCTGAACGAGGAGATGTTCGACAGCCTGGAGGATGCCCGCCGAAAACTGGCGCTCTGGCGTTACGACTACAACAACGTCAGGCCGCACTCAT
CTCTTGCGAACCAAACACCCGTAGAAGCGCGTCGAGCGCTTGAGAAATTTGAAGGCTCCGCGCCCGACGCGCTTGCCCAAACCGAAACCGAGGAATATGA
AATCCGAACCCGCAAACTCTCGTTATGAACGCGGGAGCCTCGGGGGGCAGGTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
264 bp87 aa69332+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRKSRFTEAQIIGMIKEQEAGMPTAEVCRKHGLSQGTFYKFKSKYGGMEVSDAARLKALEDENAKLKRLLADTMLDNVVLKDLLGKS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
945 bp314 aa2841228+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

YNAGQRCAEGLAGKELTTPTKRRAAALRAMREHDISQRRVCRLVGVDPKTVRRDRPPDNPEIRKEMKAIANTRRRFGYRRIGVMLERKGMIMNHKKLYRL
YTEEKLGVRRRRGRKRARGSRTPMPEALKPGERWSLDFLSDTFGASRRFRILAVNDDCCRENLCLMADTGISGARVARELDALVRIYGKPASIVSDNGTE
FTSRAILKWANDNGVDWHYIDPGKPQQNAFIESFNGSLRDELLNEEMFDSLEDARRKLALWRYDYNNVRPHSSLANQTPVEARRALEKFEGSAPDALAQT
ETEEYEIRTRKLSL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1160 bp386 aa691228+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKSRFTEAQIIGMIKEQEAGMPTAEVCRKHGLSQGTFYKFKSKYGGMEVSDAARLKALEDENAKLKRLLADTMLDNVVLKDLLGKELTTPTKRRAAALR
AMREHDISQRRVCRLVGVDPKTVRRDRPPDNPEIRKEMKAIANTRRRFGYRRIGVMLERKGMIMNHKKLYRLYTEEKLGVRRRRGRKRARGSRTPMPEAL
KPGERWSLDFLSDTFGASRRFRILAVNDDCCRENLCLMADTGISGARVARELDALVRIYGKPASIVSDNGTEFTSRAILKWANDNGVDWHYIDPGKPQQN
AFIESFNGSLRDELLNEEMFDSLEDARRKLALWRYDYNNVRPHSSLANQTPVEARRALEKFEGSAPDALAQTETEEYEIRTRKLSL

 

Blast result :
Comments
Genome in project .
ISRosp2 is 91 % (ORFA) aa similar to ISR1 and 67% (ORFB) aa similar to ISRpa2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Pinhassi,J., Pedros-Alio,C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.