ISLpn8

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_002942 ND Legionella pneumophila
Legionella pneumophila subsp. pneumophila
DNA section
IS Length : 1199 bp

Ends


IR Length : 32/42

IRL : TGTAACCATCCCCTAAAATAGTTGTATTCCTGATTAGAGTTCTCCAAGTT
IRR : TGTAAACCCCCCATATTTAGTGGCATCCTAAATTAGAGTTCATTGGCTGA

Insertion site


Left flankDirect repeatRight flankDR Length
TAGTTGTCTTGCTACAATTT0

DNA sequence

TGTAACCATCCCCTAAAATAGTTGTATTCCTGATTAGAGTTCTCCAAGTTAAACTACCACTAAGGAGAACGAAGATGAAAAAATCGCGTTATACAGAAAC
TCAGATTGTCAAAATTCTAAAAGAGGTTGAAGCAGGCAGACTGGTTAAAGAAATTTGTCGTGAATATGGTATTTCTGACGCAACATATTACAACTGGAAA
GCAAAATATGGAGGCATGGAGTCATCAGACATAAAGCGTCTGAAAGACCTTGAAGAAGAGAACCGTCGTCTAAAGCAGATGTACGCTGAACTGAGTCTTG
ATCACAAAATCCTGAAGGATATTGTTGAAAAAAAGCTGTGAAGCCAACTGTGCGTCGGGAGTTGGTTGATTATGCAGTCAATGCTCATGCTGTGAGTTTA
CGCCGCGCTTGCAAGGTTGTGGGTATCAACGACTCGGTTTATCGCTATAAGCCGGATAGTCGGTCTGATGAAAGTGTCATTGTGGCATTACAAGAATCAT
CTGAACGTTACCCAGCGTATGGCTTCAGTAAATTGCTTAAGGTCTTACGGCGCCAGGGGCACAAATGGAATCATAAACGGGTTTACCGTGTGTATTGTGA
GCTTAAGCTCAATATGCGTCGCAAGGGAAAAAAGCGGTTACCCAATCGCTCACCTGGACCTCTGAGTGTGCCAGCGTCCATTAACCAGTGTTGGTCAATG
GACTTTATGTGCGATGCATTGATGTGTGGTCGACGCTTCCGAACATTTAACATAGTGGATGATTTCAATCGTGAAGTGTTAGCGATCGAGATAGATTTGA
ACCTGCCCGCACAGCGAGTAATTCGCGTATTAGAACGTGTTGTTGCCTGGCGTGGTCTCCCTGCCAAACTACGTATGGATAATGGTCCTGAATTTATTTC
CAGAGCCCTAGCTGATTGGGCAGAAAAAAATAAGGTGCAGTTGGAATTTATCAAGCCAGCCAAGCCAACACAAAATTCATTTATTGAGCGATTCAATAGA
ACTTATCGCACAGAAATACTGGATATGTATGCGTTCAAAAATTTACACGAAGTAAGAGAGTTAACAGAAAACTGGATAAAGGAGTACAACGACGAAAGAC
CCCATGATTCACTAAATGATTTAACGCCGTGGGAATATCTGGCAAAAAATCAGCCAATGAACTCTAATTTAGGATGCCACTAAATATGGGGGGTTTACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa75341+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKSRYTETQIVKILKEVEAGRLVKEICREYGISDATYYNWKAKYGGMESSDIKRLKDLEEENRRLKQMYAELSLDHKILKDIVEKKL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
855 bp284 aa3291183+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKAVKPTVRRELVDYAVNAHAVSLRRACKVVGINDSVYRYKPDSRSDESVIVALQESSERYPAYGFSKLLKVLRRQGHKWNHKRVYRVYCELKLNMRRKG
KKRLPNRSPGPLSVPASINQCWSMDFMCDALMCGRRFRTFNIVDDFNREVLAIEIDLNLPAQRVIRVLERVVAWRGLPAKLRMDNGPEFISRALADWAEK
NKVQLEFIKPAKPTQNSFIERFNRTYRTEILDMYAFKNLHEVRELTENWIKEYNDERPHDSLNDLTPWEYLAKNQPMNSNLGCH

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1109 bp370 aa751183+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKSRYTETQIVKILKEVEAGRLVKEICREYGISDATYYNWKAKYGGMESSDIKRLKDLEEENRRLKQMYAELSLDHKILKDIVEKKAVMKPTVRRELVD
YAVNAHAVSLRRACKVVGINDSVYRYKPDSRSDESVIVALQESSERYPAYGFSKLLKVLRRQGHKWNHKRVYRVYCELKLNMRRKGKKRLPNRSPGPLSV
PASINQCWSMDFMCDALMCGRRFRTFNIVDDFNREVLAIEIDLNLPAQRVIRVLERVVAWRGLPAKLRMDNGPEFISRALADWAEKNKVQLEFIKPAKPT
QNSFIERFNRTYRTEILDMYAFKNLHEVRELTENWIKEYNDERPHDSLNDLTPWEYLAKNQPMNSNLGCH

 

Blast result :
Comments
ISLpn8 is 92 % (ORFA) aa similar to IS1351 and 78% (ORFB) aa similar to ISD1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chien,M., Morozova,I., Shi,S., Sheng,H., Chen,J., Gomez,S.M., Asamani,G., Hill,K., Nuara,J., Feder,M., Rineer,J., Greenberg,J.J., Steshenko,V., Park,S.H., Zhao,B., Teplitskaya,E., Edwards,J.R., Pampou,S., Georghiou,A., Chou,I.C., Iannuccilli,W., Ulz,M.E., Kim,D.H., Geringer-Sameth,A., Goldsberry,C., Morozov,P., Fischer,S.G., Segal,G., Qu,X., Rzhetsky,A., Zhang,P., Cayanis,E., De Jong,P.J., Ju,J., Kalachikov,S., Shuman,H.A. and Russo,J.J. (2004) Science 305 (5692), 1966-1968.