ISYps8
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006153 | ND | Yersinia pseudotuberculosis | Yersinia pseudotuberculosis IP 32953 |
DNA section
IS Length : 1212 bp
Ends
IR Length : 23/40
IRL : TGATCTTCCCCCTTCGTTCAGTACCGCTGTAAAGTAGAGTTTTCGGCCCT
IRR : TGATCCCTCCCCAATATCCGTACCAGGCTAAATCAGAGATCCGGACCTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGGATTGCT | AATTTGCCTG | 0 |
DNA sequence
TGATCTTCCCCCTTCGTTCAGTACCGCTGTAAAGTAGAGTTTTCGGCCCTTCTTTTCCGTCCAAAAAGGGGTCATTCGTTATGAAAAAGACGCGTTATAC
CGAAGAACAAATCGCTTTTGCACTCAAACAGGCTGAAACGGGTACTCGAGTGGAAGAAGTCTGTAGGAAGATGGGCATTTCGGAAGCGACATTTTACAAC
TGGAAGAAGAAGTTTGGGGGAATGGGTGTCACGGAATTGCGCCGTCTTCGGCAGCTTGAGGATGAGAATCAGCGCCTCAAACGTCTGGTGGCCGATCTGA
GTCTGGATAAGGAAATGCTGCAGGATGTCATCCGAAAAAAGTTCTGAGGCCGCTGCAGAAGCGCGATGCCGTGGAATATCTGCTGGCGGCGTACCGTATC
GGAGTACGCAGAGGGTGCAGGCTGATGATGCAGAGCCGCACTGTATACAACTATCGTAGCCATCGTGACGATCGGGCTATTACTCAGCGAATACGGGAAA
TTGCGGAGACGCGCATCCGTTATGGCTGTCCGCGCATTCATATCCTGCTGCGGAGAGAAGGCTGGCTTGTTAACCATAAGAAAACGCACCGTATTTACTG
TCTTGAGGGTCTCAATCTACGTTCGAAACGTCCACGGCGGCATGTGACAGCGAAGCACAGGCACGCACGTCCAGAAGTGACCGCGTTAGAGCAGTGCTGG
AGCATGGATTTCGTTGCTGATAATCTGTTCAACGGGCGTCGGGTCAGGGCGCTAACTATAGTGGATAATTTTAGTCGCGAATGTCTGGCGCTCGAGGTCG
GTCAGGGGTTACGTGGAGATGATGTTGTGGCTGTCATGGACAGATTAAAACATTCGCTGGGGCGTATTCCACAAAGGCTGCAGACAGATAACGGCAGCGA
ATTCATCTCGAAGTCGATGGACCGATGGGCGTATGAAAACAGGGTCACGATGGACTTTTCACGCCCCGGAAAGCCTACAGATAATGCCTTTATCGAGTCA
TTTAATGGCAGTCTGAGGGATGAATGTCTGAACGTGCACGGGTTCCTTTCTCTGGAAGATGCTCAGGAGAAAATTGAACAATGGCGGCAAGAATATAATC
ATTTTCGTCCGCATTCTTCGTTAAATAACCTGACTCCGGCAGAATTTGCCCGAAGTCATCAAAAAGGTCCGGATCTCTGATTTAGCCTGGTACGGATATT
GGGGAGGGATCA
CGAAGAACAAATCGCTTTTGCACTCAAACAGGCTGAAACGGGTACTCGAGTGGAAGAAGTCTGTAGGAAGATGGGCATTTCGGAAGCGACATTTTACAAC
TGGAAGAAGAAGTTTGGGGGAATGGGTGTCACGGAATTGCGCCGTCTTCGGCAGCTTGAGGATGAGAATCAGCGCCTCAAACGTCTGGTGGCCGATCTGA
GTCTGGATAAGGAAATGCTGCAGGATGTCATCCGAAAAAAGTTCTGAGGCCGCTGCAGAAGCGCGATGCCGTGGAATATCTGCTGGCGGCGTACCGTATC
GGAGTACGCAGAGGGTGCAGGCTGATGATGCAGAGCCGCACTGTATACAACTATCGTAGCCATCGTGACGATCGGGCTATTACTCAGCGAATACGGGAAA
TTGCGGAGACGCGCATCCGTTATGGCTGTCCGCGCATTCATATCCTGCTGCGGAGAGAAGGCTGGCTTGTTAACCATAAGAAAACGCACCGTATTTACTG
TCTTGAGGGTCTCAATCTACGTTCGAAACGTCCACGGCGGCATGTGACAGCGAAGCACAGGCACGCACGTCCAGAAGTGACCGCGTTAGAGCAGTGCTGG
AGCATGGATTTCGTTGCTGATAATCTGTTCAACGGGCGTCGGGTCAGGGCGCTAACTATAGTGGATAATTTTAGTCGCGAATGTCTGGCGCTCGAGGTCG
GTCAGGGGTTACGTGGAGATGATGTTGTGGCTGTCATGGACAGATTAAAACATTCGCTGGGGCGTATTCCACAAAGGCTGCAGACAGATAACGGCAGCGA
ATTCATCTCGAAGTCGATGGACCGATGGGCGTATGAAAACAGGGTCACGATGGACTTTTCACGCCCCGGAAAGCCTACAGATAATGCCTTTATCGAGTCA
TTTAATGGCAGTCTGAGGGATGAATGTCTGAACGTGCACGGGTTCCTTTCTCTGGAAGATGCTCAGGAGAAAATTGAACAATGGCGGCAAGAATATAATC
ATTTTCGTCCGCATTCTTCGTTAAATAACCTGACTCCGGCAGAATTTGCCCGAAGTCATCAAAAAGGTCCGGATCTCTGATTTAGCCTGGTACGGATATT
GGGGAGGGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 81 | 347 | + | No |
Description : First part of the transposase
ORF sequence :
MKKTRYTEEQIAFALKQAETGTRVEEVCRKMGISEATFYNWKKKFGGMGVTELRRLRQLEDENQRLKRLVADLSLDKEMLQDVIRKKF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 870 bp | 289 aa | 311 | 1180 | + | No |
Description : Second part of the transposase
ORF sequence :
GNAAGCHPKKVLRPLQKRDAVEYLLAAYRIGVRRGCRLMMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEG
LNLRSKRPRRHVTAKHRHARPEVTALEQCWSMDFVADNLFNGRRVRALTIVDNFSRECLALEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFIS
KSMDRWAYENRVTMDFSRPGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPHSSLNNLTPAEFARSHQKGPDL
LNLRSKRPRRHVTAKHRHARPEVTALEQCWSMDFVADNLFNGRRVRALTIVDNFSRECLALEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFIS
KSMDRWAYENRVTMDFSRPGKPTDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPHSSLNNLTPAEFARSHQKGPDL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1100 bp | 367 aa | 81 | 1180 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKTRYTEEQIAFALKQAETGTRVEEVCRKMGISEATFYNWKKKFGGMGVTELRRLRQLEDENQRLKRLVADLSLDKEMLQDVIRKKVLRPLQKRDAVME
YLLAAYRIGVRRGCRLMMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEGLNLRSKRPRRHVTAKHRHARPE
VTALEQCWSMDFVADNLFNGRRVRALTIVDNFSRECLALEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFISKSMDRWAYENRVTMDFSRPGKP
TDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPHSSLNNLTPAEFARSHQKGPDL
YLLAAYRIGVRRGCRLMMQSRTVYNYRSHRDDRAITQRIREIAETRIRYGCPRIHILLRREGWLVNHKKTHRIYCLEGLNLRSKRPRRHVTAKHRHARPE
VTALEQCWSMDFVADNLFNGRRVRALTIVDNFSRECLALEVGQGLRGDDVVAVMDRLKHSLGRIPQRLQTDNGSEFISKSMDRWAYENRVTMDFSRPGKP
TDNAFIESFNGSLRDECLNVHGFLSLEDAQEKIEQWRQEYNHFRPHSSLNNLTPAEFARSHQKGPDL
Blast result :
Comments
ISYps8 is 93% (ORFA) and 74% (ORFB) aa similar to ISRso12. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chain,P.S., Carniel,E., Larimer,F.W., Lamerdin,J., Stoutland,P.O., Regala,W.M., Georgescu,A.M., Vergez,L.M., Land,M.L., Motin,V.L., Brubaker,R.R., Fowler,J., Hinnebusch,J., Marceau,M., Medigue,C., Simonet,M., Chenal-Francisque,V., Souza,B., Dacheux,D., Elliott,J.M., Derbise,A., Hauser,L.J. and Garcia,E. (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (38), 13826-13831