ISSme1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Sinorhizobium medicae | Sinorhizobium medicae WSM419 |
DNA section
IS Length : 1271 bp
Ends
IR Length : 35/46
IRL : TGACCTGCCACGGGTAATTTCCTCCAGTTTGGATTAGAGTCCGGCCTATT
IRR : TGACCTGCCACTGAACTTTCATCCAGCGGCGATTAGAGCCTCGGCGGTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCCTGAGGG | AAGCAGTCGG | 0 |
DNA sequence
TGACCTGCCACGGGTAATTTCCTCCAGTTTGGATTAGAGTCCGGCCTATTGAAGGACGGACGCATGAAGAAGCAGAGATTTACGGAAGAGCAGATTATTG
CGGTACTGAAGGAGCAGGAGGCTGGCGCGAAGGTGGCGGACCTCTGCCGCAAGCATGGGATTTCGGACGCGACATTTTACAATTGGAAAGCCAAATTCGG
GGGCATGGAGGTTTCCGAGGCGAAGCGCCTGAAGGCGCTCGAAGAGGAGAACGCCAAGCTGAAGAAGCTGCTCGCCGAGCAGATGCTGGATGCCGCCGCA
CTCCGCGAGCTTCTTGCAAAAAAATGGTAGGGCCCGCCGCCAAGCGTGAAGCCGTCGCGCATCTGAAGGCCGTCATGGGCCTCTCGGAGCGGCGGGCCTG
CCAGATCATCTCCGCTGACCGGAAGATGGTGCGCTATCGGTCACGCCGACCGTCAGAGGCCGAACTGCGGAAGAAGCTTCGCGACCTCGCCAACGAGCGA
CGGCGTTTCGGCTACCGGCGACTGTTCGTCCTGCTCAGGCGGGAGGGAGAGCCGTCCGGCGTCAACCGCATCTATCGGCTGTATCGCGAGGAAGGGCTTT
CGGTTCGCAAGCGGAAAGCCAGGCGGCGTGCCGTCGGCACGCGTGCGCCGATACTGGTCGAAGCGAAGGCCAATGCCCGCTGGTCGCTGGATTTCGTCCA
TGATCAGTTCGCCTGCGGAAGAAGATTTCGCGTGCTCAACATCGTCGATGACGTGACGCGCGAATGCCTGGCAGCGATCCCCGACACCTCGATCTCCGGC
CGTCGTGTTGCTCGCGAATTGACGGCGCTCCTCGAGCGGCGCGGCAAGCCCGGCATGATTGTCTCCGACCATGGCACGGAATTCACGTCGAACGCGATCC
TCGCCTGGTCAAAGGAGCACAAGGTCGAGTGGCACTACATCGCACCGGGAAAGCCGATGCAGAACGGTTATATCGAGTCCTTCAACGGGCGGATGCGCGA
CGAGCTGCTGAACGAAAGCCTGTTCTTCGGTCTCGATCATGCCCGCAGCGCCATCGCCGAATGGGCAGACGATTATAACCATTCCCGGCCGCACTCATCG
CTCGGATATCAGACCCCGGCACGCTATGCCGGAACCATCGCCGCAACCGGCTTCAACGCTGTGCAAGATGAAGGCTACGCGTTTCCGCCGGTTGCTCCCA
CCGCGCCAAGTGGCGTAATGAAAACCGCCGAGGCTCTAATCGCCGCTGGATGAAAGTTCAGTGGCAGGTCA
CGGTACTGAAGGAGCAGGAGGCTGGCGCGAAGGTGGCGGACCTCTGCCGCAAGCATGGGATTTCGGACGCGACATTTTACAATTGGAAAGCCAAATTCGG
GGGCATGGAGGTTTCCGAGGCGAAGCGCCTGAAGGCGCTCGAAGAGGAGAACGCCAAGCTGAAGAAGCTGCTCGCCGAGCAGATGCTGGATGCCGCCGCA
CTCCGCGAGCTTCTTGCAAAAAAATGGTAGGGCCCGCCGCCAAGCGTGAAGCCGTCGCGCATCTGAAGGCCGTCATGGGCCTCTCGGAGCGGCGGGCCTG
CCAGATCATCTCCGCTGACCGGAAGATGGTGCGCTATCGGTCACGCCGACCGTCAGAGGCCGAACTGCGGAAGAAGCTTCGCGACCTCGCCAACGAGCGA
CGGCGTTTCGGCTACCGGCGACTGTTCGTCCTGCTCAGGCGGGAGGGAGAGCCGTCCGGCGTCAACCGCATCTATCGGCTGTATCGCGAGGAAGGGCTTT
CGGTTCGCAAGCGGAAAGCCAGGCGGCGTGCCGTCGGCACGCGTGCGCCGATACTGGTCGAAGCGAAGGCCAATGCCCGCTGGTCGCTGGATTTCGTCCA
TGATCAGTTCGCCTGCGGAAGAAGATTTCGCGTGCTCAACATCGTCGATGACGTGACGCGCGAATGCCTGGCAGCGATCCCCGACACCTCGATCTCCGGC
CGTCGTGTTGCTCGCGAATTGACGGCGCTCCTCGAGCGGCGCGGCAAGCCCGGCATGATTGTCTCCGACCATGGCACGGAATTCACGTCGAACGCGATCC
TCGCCTGGTCAAAGGAGCACAAGGTCGAGTGGCACTACATCGCACCGGGAAAGCCGATGCAGAACGGTTATATCGAGTCCTTCAACGGGCGGATGCGCGA
CGAGCTGCTGAACGAAAGCCTGTTCTTCGGTCTCGATCATGCCCGCAGCGCCATCGCCGAATGGGCAGACGATTATAACCATTCCCGGCCGCACTCATCG
CTCGGATATCAGACCCCGGCACGCTATGCCGGAACCATCGCCGCAACCGGCTTCAACGCTGTGCAAGATGAAGGCTACGCGTTTCCGCCGGTTGCTCCCA
CCGCGCCAAGTGGCGTAATGAAAACCGCCGAGGCTCTAATCGCCGCTGGATGAAAGTTCAGTGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 64 | 330 | + | No |
Description :
ORF sequence :
MKKQRFTEEQIIAVLKEQEAGAKVADLCRKHGISDATFYNWKAKFGGMEVSEAKRLKALEEENAKLKKLLAEQMLDAAALRELLAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1236 bp | 411 aa | 18 | 1253 | + | No |
Description :
ORF sequence :
FPPVWIRVRPIEGRTHEEAEIYGRADYCGTEGAGGWREGGGPLPQAWDFGRDILQLESQIRGHGGFRGEAPEGARRGERQAEEAARRADAGCRRTPRASC
KKMVGPAAKREAVAHLKAVMGLSERRACQIISADRKMVRYRSRRPSEAELRKKLRDLANERRRFGYRRLFVLLRREGEPSGVNRIYRLYREEGLSVRKRK
ARRRAVGTRAPILVEAKANARWSLDFVHDQFACGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELTALLERRGKPGMIVSDHGTEFTSNAILAWSKE
HKVEWHYIAPGKPMQNGYIESFNGRMRDELLNESLFFGLDHARSAIAEWADDYNHSRPHSSLGYQTPARYAGTIAATGFNAVQDEGYAFPPVAPTAPSGV
MKTAEALIAAG
KKMVGPAAKREAVAHLKAVMGLSERRACQIISADRKMVRYRSRRPSEAELRKKLRDLANERRRFGYRRLFVLLRREGEPSGVNRIYRLYREEGLSVRKRK
ARRRAVGTRAPILVEAKANARWSLDFVHDQFACGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELTALLERRGKPGMIVSDHGTEFTSNAILAWSKE
HKVEWHYIAPGKPMQNGYIESFNGRMRDELLNESLFFGLDHARSAIAEWADDYNHSRPHSSLGYQTPARYAGTIAATGFNAVQDEGYAFPPVAPTAPSGV
MKTAEALIAAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 396 aa | 64 | 1253 | + | Yes |
Description :
ORF sequence :
MKKQRFTEEQIIAVLKEQEAGAKVADLCRKHGISDATFYNWKAKFGGMEVSEAKRLKALEEENAKLKKLLAEQMLDAAALRELLAKKMVGPAAKREAVAH
LKAVMGLSERRACQIISADRKMVRYRSRRPSEAELRKKLRDLANERRRFGYRRLFVLLRREGEPSGVNRIYRLYREEGLSVRKRKARRRAVGTRAPILVE
AKANARWSLDFVHDQFACGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELTALLERRGKPGMIVSDHGTEFTSNAILAWSKEHKVEWHYIAPGKPMQ
NGYIESFNGRMRDELLNESLFFGLDHARSAIAEWADDYNHSRPHSSLGYQTPARYAGTIAATGFNAVQDEGYAFPPVAPTAPSGVMKTAEALIAAG
LKAVMGLSERRACQIISADRKMVRYRSRRPSEAELRKKLRDLANERRRFGYRRLFVLLRREGEPSGVNRIYRLYREEGLSVRKRKARRRAVGTRAPILVE
AKANARWSLDFVHDQFACGRRFRVLNIVDDVTRECLAAIPDTSISGRRVARELTALLERRGKPGMIVSDHGTEFTSNAILAWSKEHKVEWHYIAPGKPMQ
NGYIESFNGRMRDELLNESLFFGLDHARSAIAEWADDYNHSRPHSSLGYQTPARYAGTIAATGFNAVQDEGYAFPPVAPTAPSGVMKTAEALIAAG
Blast result :
Comments
Genome in progress : NZ_AATG01000001.
ISSme1 is 90 % (ORFA) aa similar to ISOba1 and 85% (ORFB) aa similar to IS511. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSme1 is 90 % (ORFA) aa similar to ISOba1 and 85% (ORFB) aa similar to IS511. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P.(2006) Direct Submission GenBank.