ISBcen23

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008543 ND Burkholderia cenocepacia
Burkholderia cenocepacia HI2424
DNA section
IS Length : 1195 bp

Ends


IR Length : 31/41

IRL : TGACCTGAGACGGATCGTGTAGCCAAACGGCAAGTGAGTTCTCTTATCCT
IRR : TGACCTGCGGTGGGATCTTGTAGCCAGTTAAGCAGTGAGTCGCCTGTCGA

Insertion site


Left flankDirect repeatRight flankDR Length
CATTTGTGTCCAGGATTTCTAGAT4

DNA sequence

TGACCTGAGACGGATCGTGTAGCCAAACGGCAAGTGAGTTCTCTTATCCTAGAGGCAAGGAGAACTCGATGAAGAAGCGACATACCGACGAACAGATCAT
CCGCATCCTGCGGGAAGCCGAGAGCCAGGAGGTACCGGTCAAGGATCTGTGCAAGCGGCACAACATCTCGGAGCAGACGTTCTACCGGTGGCGCACCAAG
TTCGGTGGCATGGACGTGGCCGAAGCACGTCGGCTCAAGGAACTGGAATCGGAGAACGAGAAGCTCAAGCGGCTGGTGGCCGAGCAATTGCTGGTGATCG
ACGGCTTGAAGGAGTTCAGCCGAAAAAAATGAATTCCCCGACGGGCCGGCGCGAAGCGCTGGAAGTCCTGACTCGGCGGGGATTATCGCAACGCAAGGCA
TGTCGTTACCTGGGGCTGAGCCGCCGGGTGGCGATCTACACGCTCAAGCAACCGGAGAAAGACCGGAGCTTGGGCGAGCGGTTGATCGCGGCTTCCCAAG
AGGTGCCACGCTTCGGTTATCGTCGGATCTCGGCTTGGCTGTCGTTGGGCGAATCGCGTGTGCGCCGGATGTGGCGTGCGCTGAAGCTGAACATCCCGAA
GCGCCGGCCGCGTCGACGGCGTTGCGGCAGCGATATTCGCCTGCCGGGTGCGACGAAACCCAATTCGGTCTGGAGCTACGACTTCGTCCACGACCAGTTG
GTCGACGGTCGAGTCTTGAAGATGCTGTGCGTGATCGACGAATACACCCGCGAATGCCTAGCAATCGAGGTCGGGGCCAGCTTGCGTTCGCAAGACGTGA
TCCTGGTCCTGTCGCGATTGATGCGCCTATACGGCAAGCCGGCGTTCATTCGGTCCGACAACGGCGCGGAATTTACGGCGGCCAAGGTTATGCGCTGGCT
GCGTGACGCGGCAATCGGGCCAGCCTTCATTACGCCCGGTAACCCGTGGCAAAACGGCTTCGTCGAAAGCTTCAACGGCAAGCTGCGCGACGAGCTGCTG
AATCGGGAATGGTTCCGCAGCCGCGCCGAGGCCAAGGTGCTCATCGAGCGCTGGCGACAGTTCTACAACGAGCGTCGGCCGCATAGCGCTCATCGTTACC
AACCTCCTGCCACGGTCCGTCGAGCCTGGCTGGATTCCGAGAATATCGACAGGCGACTCACTGCTTAACTGGCTACAAGATCCCACCGCAGGTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
264 bp87 aa69332+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKRHTDEQIIRILREAESQEVPVKDLCKRHNISEQTFYRWRTKFGGMDVAEARRLKELESENEKLKRLVAEQLLVIDGLKEFSRKK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1116 bp371 aa531168+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RQGELDEEATYRRTDHPHPAGSREPGGTGQGSVQAAQHLGADVLPVAHQVRWHGRGRSTSAQGTGIGEREAQAAGGRAIAGDRRLEGVQPKKMNSPTGRR
EALEVLTRRGLSQRKACRYLGLSRRVAIYTLKQPEKDRSLGERLIAASQEVPRFGYRRISAWLSLGESRVRRMWRALKLNIPKRRPRRRRCGSDIRLPGA
TKPNSVWSYDFVHDQLVDGRVLKMLCVIDEYTRECLAIEVGASLRSQDVILVLSRLMRLYGKPAFIRSDNGAEFTAAKVMRWLRDAAIGPAFITPGNPWQ
NGFVESFNGKLRDELLNREWFRSRAEAKVLIERWRQFYNERRPHSAHRYQPPATVRRAWLDSENIDRRLTA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1100 bp366 aa691168+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKRHTDEQIIRILREAESQEVPVKDLCKRHNISEQTFYRWRTKFGGMDVAEARRLKELESENEKLKRLVAEQLLVIDGLKEFSRKKMNSPTGRREALEV
LTRRGLSQRKACRYLGLSRRVAIYTLKQPEKDRSLGERLIAASQEVPRFGYRRISAWLSLGESRVRRMWRALKLNIPKRRPRRRRCGSDIRLPGATKPNS
VWSYDFVHDQLVDGRVLKMLCVIDEYTRECLAIEVGASLRSQDVILVLSRLMRLYGKPAFIRSDNGAEFTAAKVMRWLRDAAIGPAFITPGNPWQNGFVE
SFNGKLRDELLNREWFRSRAEAKVLIERWRQFYNERRPHSAHRYQPPATVRRAWLDSENIDRRLTA

 

Blast result :
Comments
ISBcen23 is 78 % (ORFA) aa similar to IS1404 and 57% (ORFB) aa similar to ISBp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., LiPuma,J.J., Gonzalez,C.F., Konstantinidis,K., Tiedje,J.M. and Richardson,P.(2006) Direct Submission GenBank.