ISBcen23
- Family IS3
- Group IS407
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_008543 | ND | Burkholderia cenocepacia | Burkholderia cenocepacia HI2424 |
DNA section
IS Length : 1195 bp
Ends
IR Length : 31/41
IRL : TGACCTGAGACGGATCGTGTAGCCAAACGGCAAGTGAGTTCTCTTATCCT
IRR : TGACCTGCGGTGGGATCTTGTAGCCAGTTAAGCAGTGAGTCGCCTGTCGA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CATTTGTGTC | CAGG | ATTTCTAGAT | 4 |
DNA sequence
TGACCTGAGACGGATCGTGTAGCCAAACGGCAAGTGAGTTCTCTTATCCTAGAGGCAAGGAGAACTCGATGAAGAAGCGACATACCGACGAACAGATCAT
CCGCATCCTGCGGGAAGCCGAGAGCCAGGAGGTACCGGTCAAGGATCTGTGCAAGCGGCACAACATCTCGGAGCAGACGTTCTACCGGTGGCGCACCAAG
TTCGGTGGCATGGACGTGGCCGAAGCACGTCGGCTCAAGGAACTGGAATCGGAGAACGAGAAGCTCAAGCGGCTGGTGGCCGAGCAATTGCTGGTGATCG
ACGGCTTGAAGGAGTTCAGCCGAAAAAAATGAATTCCCCGACGGGCCGGCGCGAAGCGCTGGAAGTCCTGACTCGGCGGGGATTATCGCAACGCAAGGCA
TGTCGTTACCTGGGGCTGAGCCGCCGGGTGGCGATCTACACGCTCAAGCAACCGGAGAAAGACCGGAGCTTGGGCGAGCGGTTGATCGCGGCTTCCCAAG
AGGTGCCACGCTTCGGTTATCGTCGGATCTCGGCTTGGCTGTCGTTGGGCGAATCGCGTGTGCGCCGGATGTGGCGTGCGCTGAAGCTGAACATCCCGAA
GCGCCGGCCGCGTCGACGGCGTTGCGGCAGCGATATTCGCCTGCCGGGTGCGACGAAACCCAATTCGGTCTGGAGCTACGACTTCGTCCACGACCAGTTG
GTCGACGGTCGAGTCTTGAAGATGCTGTGCGTGATCGACGAATACACCCGCGAATGCCTAGCAATCGAGGTCGGGGCCAGCTTGCGTTCGCAAGACGTGA
TCCTGGTCCTGTCGCGATTGATGCGCCTATACGGCAAGCCGGCGTTCATTCGGTCCGACAACGGCGCGGAATTTACGGCGGCCAAGGTTATGCGCTGGCT
GCGTGACGCGGCAATCGGGCCAGCCTTCATTACGCCCGGTAACCCGTGGCAAAACGGCTTCGTCGAAAGCTTCAACGGCAAGCTGCGCGACGAGCTGCTG
AATCGGGAATGGTTCCGCAGCCGCGCCGAGGCCAAGGTGCTCATCGAGCGCTGGCGACAGTTCTACAACGAGCGTCGGCCGCATAGCGCTCATCGTTACC
AACCTCCTGCCACGGTCCGTCGAGCCTGGCTGGATTCCGAGAATATCGACAGGCGACTCACTGCTTAACTGGCTACAAGATCCCACCGCAGGTCA
CCGCATCCTGCGGGAAGCCGAGAGCCAGGAGGTACCGGTCAAGGATCTGTGCAAGCGGCACAACATCTCGGAGCAGACGTTCTACCGGTGGCGCACCAAG
TTCGGTGGCATGGACGTGGCCGAAGCACGTCGGCTCAAGGAACTGGAATCGGAGAACGAGAAGCTCAAGCGGCTGGTGGCCGAGCAATTGCTGGTGATCG
ACGGCTTGAAGGAGTTCAGCCGAAAAAAATGAATTCCCCGACGGGCCGGCGCGAAGCGCTGGAAGTCCTGACTCGGCGGGGATTATCGCAACGCAAGGCA
TGTCGTTACCTGGGGCTGAGCCGCCGGGTGGCGATCTACACGCTCAAGCAACCGGAGAAAGACCGGAGCTTGGGCGAGCGGTTGATCGCGGCTTCCCAAG
AGGTGCCACGCTTCGGTTATCGTCGGATCTCGGCTTGGCTGTCGTTGGGCGAATCGCGTGTGCGCCGGATGTGGCGTGCGCTGAAGCTGAACATCCCGAA
GCGCCGGCCGCGTCGACGGCGTTGCGGCAGCGATATTCGCCTGCCGGGTGCGACGAAACCCAATTCGGTCTGGAGCTACGACTTCGTCCACGACCAGTTG
GTCGACGGTCGAGTCTTGAAGATGCTGTGCGTGATCGACGAATACACCCGCGAATGCCTAGCAATCGAGGTCGGGGCCAGCTTGCGTTCGCAAGACGTGA
TCCTGGTCCTGTCGCGATTGATGCGCCTATACGGCAAGCCGGCGTTCATTCGGTCCGACAACGGCGCGGAATTTACGGCGGCCAAGGTTATGCGCTGGCT
GCGTGACGCGGCAATCGGGCCAGCCTTCATTACGCCCGGTAACCCGTGGCAAAACGGCTTCGTCGAAAGCTTCAACGGCAAGCTGCGCGACGAGCTGCTG
AATCGGGAATGGTTCCGCAGCCGCGCCGAGGCCAAGGTGCTCATCGAGCGCTGGCGACAGTTCTACAACGAGCGTCGGCCGCATAGCGCTCATCGTTACC
AACCTCCTGCCACGGTCCGTCGAGCCTGGCTGGATTCCGAGAATATCGACAGGCGACTCACTGCTTAACTGGCTACAAGATCCCACCGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
264 bp | 87 aa | 69 | 332 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRHTDEQIIRILREAESQEVPVKDLCKRHNISEQTFYRWRTKFGGMDVAEARRLKELESENEKLKRLVAEQLLVIDGLKEFSRKK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1116 bp | 371 aa | 53 | 1168 | + | No |
Description : Second part of the transposase
ORF sequence :
RQGELDEEATYRRTDHPHPAGSREPGGTGQGSVQAAQHLGADVLPVAHQVRWHGRGRSTSAQGTGIGEREAQAAGGRAIAGDRRLEGVQPKKMNSPTGRR
EALEVLTRRGLSQRKACRYLGLSRRVAIYTLKQPEKDRSLGERLIAASQEVPRFGYRRISAWLSLGESRVRRMWRALKLNIPKRRPRRRRCGSDIRLPGA
TKPNSVWSYDFVHDQLVDGRVLKMLCVIDEYTRECLAIEVGASLRSQDVILVLSRLMRLYGKPAFIRSDNGAEFTAAKVMRWLRDAAIGPAFITPGNPWQ
NGFVESFNGKLRDELLNREWFRSRAEAKVLIERWRQFYNERRPHSAHRYQPPATVRRAWLDSENIDRRLTA
EALEVLTRRGLSQRKACRYLGLSRRVAIYTLKQPEKDRSLGERLIAASQEVPRFGYRRISAWLSLGESRVRRMWRALKLNIPKRRPRRRRCGSDIRLPGA
TKPNSVWSYDFVHDQLVDGRVLKMLCVIDEYTRECLAIEVGASLRSQDVILVLSRLMRLYGKPAFIRSDNGAEFTAAKVMRWLRDAAIGPAFITPGNPWQ
NGFVESFNGKLRDELLNREWFRSRAEAKVLIERWRQFYNERRPHSAHRYQPPATVRRAWLDSENIDRRLTA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1100 bp | 366 aa | 69 | 1168 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRHTDEQIIRILREAESQEVPVKDLCKRHNISEQTFYRWRTKFGGMDVAEARRLKELESENEKLKRLVAEQLLVIDGLKEFSRKKMNSPTGRREALEV
LTRRGLSQRKACRYLGLSRRVAIYTLKQPEKDRSLGERLIAASQEVPRFGYRRISAWLSLGESRVRRMWRALKLNIPKRRPRRRRCGSDIRLPGATKPNS
VWSYDFVHDQLVDGRVLKMLCVIDEYTRECLAIEVGASLRSQDVILVLSRLMRLYGKPAFIRSDNGAEFTAAKVMRWLRDAAIGPAFITPGNPWQNGFVE
SFNGKLRDELLNREWFRSRAEAKVLIERWRQFYNERRPHSAHRYQPPATVRRAWLDSENIDRRLTA
LTRRGLSQRKACRYLGLSRRVAIYTLKQPEKDRSLGERLIAASQEVPRFGYRRISAWLSLGESRVRRMWRALKLNIPKRRPRRRRCGSDIRLPGATKPNS
VWSYDFVHDQLVDGRVLKMLCVIDEYTRECLAIEVGASLRSQDVILVLSRLMRLYGKPAFIRSDNGAEFTAAKVMRWLRDAAIGPAFITPGNPWQNGFVE
SFNGKLRDELLNREWFRSRAEAKVLIERWRQFYNERRPHSAHRYQPPATVRRAWLDSENIDRRLTA
Blast result :
Comments
ISBcen23 is 78 % (ORFA) aa similar to IS1404 and 57% (ORFB) aa similar to ISBp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., LiPuma,J.J., Gonzalez,C.F., Konstantinidis,K., Tiedje,J.M. and Richardson,P.(2006) Direct Submission GenBank.