ISMysp3
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008703 | ND | Mycobacterium sp. | Mycobacterium sp. KMS |
DNA section
IS Length : 1362 bp
Ends
IR Length : 27/30
IRL : TGAACCATCCCGCGTTTGATGCGCACCTCCTTCCTTGGGAAGGTGCATGT
IRR : TGAACCGTCCCGGGTTTCATGCACCCTCAGGTTTCTGTGATCCGGCCGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGTGATCTCC | AGG | TGCCGGCACA | 3 |
DNA sequence
TGAACCATCCCGCGTTTGATGCGCACCTCCTTCCTTGGGAAGGTGCATGTCATGCCGAGCAAGTACGACCCGGAGACCCGAGCGAAGGCGGTCCGTCTGG
TGCGCGAGCACCGGGAGGACTATCCGAGCGAGTGGGCGGCGATTACCGCGGTGTCCAAACGGTTGGGGATGAACGCCGAGACGCTGCGTAATTGGATTCG
CCAGCAACAGGTCGATGACGGTGATCGAGACGGGGTCTCTTCGGAGGCGGCTGCGGAGATCCGGGCGTTGAAACGGCGTAACGCCGAGCTGGAGCAGACT
ATCGAAATCCTCAAGGCGGCAACGTCTTTCTTCGTGCGGGAGAGCGACCCGCGCAACCGCCGCTGACCGCTACGGTCTGCGAGTTCATCGCCGCCCACCG
GGACCGTTTCGGGGTCGCTCCGATCTGCCGCGTGCTCACCGAGCACGCCGTGCCGATCGCCCCGCGGACATTTCATGCCTGGGCGGTGCGGGCGCCCTCG
AAACGGGCCCTGTGGGACGCCACGATCACCGAGATCCTAGCCGGCTACTACGAGCCTGATGAGCACGGACGCCGCAGGCCCGAGTCGCTGTACGGGTCGC
TGAAGATGTGGGCCCATCTGCAGCGTGAGGGCATCCCGGTGGCCAAGTCCACCGTGGAACGCCTGATGCGCAAGAACGGCTGGCAAGGCGTGCGCCGCCA
GAAGAGGGTCCGCACCACGATCCCCGACCCGGAGGCGGTGCGACCGCCGGACCTGGTGGACCGCCAATTCGGGGTGGCCGCACCCAACGTGCTGCTCGTG
GCCGACTTCACCTACGTCAAGCTCGTCACCGGCGTGTTCGTCTACGTCGCGTTCGTCATCGACGCCTACGCCGGGTCGATCGTGGGCTGGGAAGCCTCGG
CGTCCAAGCACACCCGCTTCGTCGAATCCGCGCTGCGTCAGGCCGCTGCACTGCGTGCCCGCCAAGGCCATCCCGTCGACGGCGCAATCCATCACAGCGA
CGCCGGCTCGCAGTACACGAGTGTGAGATTTGGTGAGACACTGAGCCTTTCGGGAATTCGCCCGTCCGTGGGCAGTGTTGGGGATGCCTTCGACAACGCG
CTGGCTGAGACGACCATCGGCCTGTACAAGAACGAATGTGTCCGGGCGGATTCCCCGTTTCGCCGCGGCCCACTGAACACCGTGGGCGACGTCGAATACA
TCACCGCCGACTACGTCGCCTGGTACAACCAGCAGCGCCTGATGCACCGACTCGGACGCATTCCGCCGGCCGAAGCCGAAGCCCGCTACTATTCCCAACT
CGTGACCGGTAGACCGGCCGGATCACAGAAACCTGAGGGTGCATGAAACCCGGGACGGTTCA
TGCGCGAGCACCGGGAGGACTATCCGAGCGAGTGGGCGGCGATTACCGCGGTGTCCAAACGGTTGGGGATGAACGCCGAGACGCTGCGTAATTGGATTCG
CCAGCAACAGGTCGATGACGGTGATCGAGACGGGGTCTCTTCGGAGGCGGCTGCGGAGATCCGGGCGTTGAAACGGCGTAACGCCGAGCTGGAGCAGACT
ATCGAAATCCTCAAGGCGGCAACGTCTTTCTTCGTGCGGGAGAGCGACCCGCGCAACCGCCGCTGACCGCTACGGTCTGCGAGTTCATCGCCGCCCACCG
GGACCGTTTCGGGGTCGCTCCGATCTGCCGCGTGCTCACCGAGCACGCCGTGCCGATCGCCCCGCGGACATTTCATGCCTGGGCGGTGCGGGCGCCCTCG
AAACGGGCCCTGTGGGACGCCACGATCACCGAGATCCTAGCCGGCTACTACGAGCCTGATGAGCACGGACGCCGCAGGCCCGAGTCGCTGTACGGGTCGC
TGAAGATGTGGGCCCATCTGCAGCGTGAGGGCATCCCGGTGGCCAAGTCCACCGTGGAACGCCTGATGCGCAAGAACGGCTGGCAAGGCGTGCGCCGCCA
GAAGAGGGTCCGCACCACGATCCCCGACCCGGAGGCGGTGCGACCGCCGGACCTGGTGGACCGCCAATTCGGGGTGGCCGCACCCAACGTGCTGCTCGTG
GCCGACTTCACCTACGTCAAGCTCGTCACCGGCGTGTTCGTCTACGTCGCGTTCGTCATCGACGCCTACGCCGGGTCGATCGTGGGCTGGGAAGCCTCGG
CGTCCAAGCACACCCGCTTCGTCGAATCCGCGCTGCGTCAGGCCGCTGCACTGCGTGCCCGCCAAGGCCATCCCGTCGACGGCGCAATCCATCACAGCGA
CGCCGGCTCGCAGTACACGAGTGTGAGATTTGGTGAGACACTGAGCCTTTCGGGAATTCGCCCGTCCGTGGGCAGTGTTGGGGATGCCTTCGACAACGCG
CTGGCTGAGACGACCATCGGCCTGTACAAGAACGAATGTGTCCGGGCGGATTCCCCGTTTCGCCGCGGCCCACTGAACACCGTGGGCGACGTCGAATACA
TCACCGCCGACTACGTCGCCTGGTACAACCAGCAGCGCCTGATGCACCGACTCGGACGCATTCCGCCGGCCGAAGCCGAAGCCCGCTACTATTCCCAACT
CGTGACCGGTAGACCGGCCGGATCACAGAAACCTGAGGGTGCATGAAACCCGGGACGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 315 bp | 104 aa | 52 | 366 | + | No |
Description :
ORF sequence :
MPSKYDPETRAKAVRLVREHREDYPSEWAAITAVSKRLGMNAETLRNWIRQQQVDDGDRDGVSSEAAAEIRALKRRNAELEQTIEILKAATSFFVRESDP
RNRR
RNRR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1065 bp | 354 aa | 282 | 1346 | + | No |
Description :
ORF sequence :
RRAGADYRNPQGGNVFLRAGERPAQPPLTATVCEFIAAHRDRFGVAPICRVLTEHAVPIAPRTFHAWAVRAPSKRALWDATITEILAGYYEPDEHGRRRP
ESLYGSLKMWAHLQREGIPVAKSTVERLMRKNGWQGVRRQKRVRTTIPDPEAVRPPDLVDRQFGVAAPNVLLVADFTYVKLVTGVFVYVAFVIDAYAGSI
VGWEASASKHTRFVESALRQAAALRARQGHPVDGAIHHSDAGSQYTSVRFGETLSLSGIRPSVGSVGDAFDNALAETTIGLYKNECVRADSPFRRGPLNT
VGDVEYITADYVAWYNQQRLMHRLGRIPPAEAEARYYSQLVTGRPAGSQKPEGA
ESLYGSLKMWAHLQREGIPVAKSTVERLMRKNGWQGVRRQKRVRTTIPDPEAVRPPDLVDRQFGVAAPNVLLVADFTYVKLVTGVFVYVAFVIDAYAGSI
VGWEASASKHTRFVESALRQAAALRARQGHPVDGAIHHSDAGSQYTSVRFGETLSLSGIRPSVGSVGDAFDNALAETTIGLYKNECVRADSPFRRGPLNT
VGDVEYITADYVAWYNQQRLMHRLGRIPPAEAEARYYSQLVTGRPAGSQKPEGA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1295 bp | 431 aa | 52 | 1346 | + | Yes |
Description :
ORF sequence :
MPSKYDPETRAKAVRLVREHREDYPSEWAAITAVSKRLGMNAETLRNWIRQQQVDDGDRDGVSSEAAAEIRALKRRNAELEQTIEILKAATSFLRAGERP
AQPPLTATVCEFIAAHRDRFGVAPICRVLTEHAVPIAPRTFHAWAVRAPSKRALWDATITEILAGYYEPDEHGRRRPESLYGSLKMWAHLQREGIPVAKS
TVERLMRKNGWQGVRRQKRVRTTIPDPEAVRPPDLVDRQFGVAAPNVLLVADFTYVKLVTGVFVYVAFVIDAYAGSIVGWEASASKHTRFVESALRQAAA
LRARQGHPVDGAIHHSDAGSQYTSVRFGETLSLSGIRPSVGSVGDAFDNALAETTIGLYKNECVRADSPFRRGPLNTVGDVEYITADYVAWYNQQRLMHR
LGRIPPAEAEARYYSQLVTGRPAGSQKPEGA
AQPPLTATVCEFIAAHRDRFGVAPICRVLTEHAVPIAPRTFHAWAVRAPSKRALWDATITEILAGYYEPDEHGRRRPESLYGSLKMWAHLQREGIPVAKS
TVERLMRKNGWQGVRRQKRVRTTIPDPEAVRPPDLVDRQFGVAAPNVLLVADFTYVKLVTGVFVYVAFVIDAYAGSIVGWEASASKHTRFVESALRQAAA
LRARQGHPVDGAIHHSDAGSQYTSVRFGETLSLSGIRPSVGSVGDAFDNALAETTIGLYKNECVRADSPFRRGPLNTVGDVEYITADYVAWYNQQRLMHR
LGRIPPAEAEARYYSQLVTGRPAGSQKPEGA
Blast result :
Comments
ISMysp3 is 68 % (ORFA) aa similar to ISPst4 and 58% (ORFB) aa similar to IS987 The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Miller,C.D. and Richardson,P.(2006) Direct submission GenBank.