ISYen3
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008800 | ND | Yersinia enterocolitica | Yersinia enterocolitica subsp. enterocolitica 8081 |
DNA section
IS Length : 1140 bp
Ends
IR Length : 36/48
IRL : TGACCTGCTCCCCGTTGATTAGTACACCCCGATATTAGTAATGTCTTCAT
IRR : TGACCTGCCCCCAGGGTTAGATACAACGCTCAGTTAGTAATGTCGGATCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTAGTGCCAA | TGTGGTTCGG | 0 |
DNA sequence
TGACCTGCTCCCCGTTGATTAGTACACCCCGATATTAGTAATGTCTTCATAAGCCACATGAGGACATCCCCATGAAGAAGCGTTTTTCCGACCAACAGAT
CATCAGTATTCTCCGCGAGGCTGAAGCCGGGGTATCCGCCCGTGAACTCTGCCGCAAGCACGCCATTTCCGATGCCACGTTTTACACCTGGCGTAAGAAG
TATGGCGGTATGGAGGTGCCTGAGGTTAAGCGCCTGAAGTCGCTTGAGGAAGAGAACGCCAGACTCAAGAAGCTTCTTGCCGAAGCCATGCTGGATAAAG
AGGCGCTTCAGGTGGCTCTTGGGCGAAAGTACTGACGACAGACCAGAAGCGGGAAGCCGTGATGTTGATGTGTGATGCGACCGGTCTGTCGCAACGTCGT
GCCTGCAGGCTAACAGGTTTGTCCCTGTCGACCTGCCGTTACGAGGCTCAGCGTCCGGCTGCTGATGCGCATTTATCAGGGCGCATCATTGAGCTGGCAC
TGGAGCGCAGGCGTTTTGGCTACCGTCGTATCTGGCAGTTGCTGCGCCGTAAAGGGCTGGCAACAGAACGTCTGCCGCTGCTCCGTCCGGCGGCGCCCAA
TCTGACCTGGTCGATGGATTTCGTCATGGACGCATTGGCCACCGGTCGCAGGATCAAGTGCCTTACCTGCGTGGACGACTTCACGAAGGAATGCCTGACG
GTCACTGTTGCCTTTGGGATTTCAGGCGTGCAGGTCACGCGTATTTTGGACAGCATTGCGCTGTTTCGCGGCTATCCGGCGACGATAAGAACTGATCAGG
GCCCGGAATTTACCTGCCGCGCGCTTGATCAATGGGCCTTTGAGCATGGCGTGGAGCTGCGGCTTATCCAGCCTGGCAAGCCGACACAGAACGGATTTAT
TGAGAGTTTTAACGGACGCTTTCGCGATGAATGCCTGAATGAACACTGGTTCAGTGACGTCAGTCATGCCAGGAAAACCATCAGTGAATGGCGTCAGGAT
TATAACGAGTGTCGCCCGCACTCCGCGCTGAATTATCAGACGCCGTCTGAATTTGCGGCGGCCTGGAGAAAGGGTAATTCTGAGAGTGAAGGATCCGACA
TTACTAACTGAGCGTTGTATCTAACCCTGGGGGCAGGTCA
CATCAGTATTCTCCGCGAGGCTGAAGCCGGGGTATCCGCCCGTGAACTCTGCCGCAAGCACGCCATTTCCGATGCCACGTTTTACACCTGGCGTAAGAAG
TATGGCGGTATGGAGGTGCCTGAGGTTAAGCGCCTGAAGTCGCTTGAGGAAGAGAACGCCAGACTCAAGAAGCTTCTTGCCGAAGCCATGCTGGATAAAG
AGGCGCTTCAGGTGGCTCTTGGGCGAAAGTACTGACGACAGACCAGAAGCGGGAAGCCGTGATGTTGATGTGTGATGCGACCGGTCTGTCGCAACGTCGT
GCCTGCAGGCTAACAGGTTTGTCCCTGTCGACCTGCCGTTACGAGGCTCAGCGTCCGGCTGCTGATGCGCATTTATCAGGGCGCATCATTGAGCTGGCAC
TGGAGCGCAGGCGTTTTGGCTACCGTCGTATCTGGCAGTTGCTGCGCCGTAAAGGGCTGGCAACAGAACGTCTGCCGCTGCTCCGTCCGGCGGCGCCCAA
TCTGACCTGGTCGATGGATTTCGTCATGGACGCATTGGCCACCGGTCGCAGGATCAAGTGCCTTACCTGCGTGGACGACTTCACGAAGGAATGCCTGACG
GTCACTGTTGCCTTTGGGATTTCAGGCGTGCAGGTCACGCGTATTTTGGACAGCATTGCGCTGTTTCGCGGCTATCCGGCGACGATAAGAACTGATCAGG
GCCCGGAATTTACCTGCCGCGCGCTTGATCAATGGGCCTTTGAGCATGGCGTGGAGCTGCGGCTTATCCAGCCTGGCAAGCCGACACAGAACGGATTTAT
TGAGAGTTTTAACGGACGCTTTCGCGATGAATGCCTGAATGAACACTGGTTCAGTGACGTCAGTCATGCCAGGAAAACCATCAGTGAATGGCGTCAGGAT
TATAACGAGTGTCGCCCGCACTCCGCGCTGAATTATCAGACGCCGTCTGAATTTGCGGCGGCCTGGAGAAAGGGTAATTCTGAGAGTGAAGGATCCGACA
TTACTAACTGAGCGTTGTATCTAACCCTGGGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 264 bp | 87 aa | 72 | 335 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRFSDQQIISILREAEAGVSARELCRKHAISDATFYTWRKKYGGMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKY
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 813 bp | 270 aa | 300 | 1112 | + | No |
Description : Second part of the transposase
ORF sequence :
RGASGGSWAKVLTTDQKREAVMLMCDATGLSQRRACRLTGLSLSTCRYEAQRPAADAHLSGRIIELALERRRFGYRRIWQLLRRKGLATERLPLLRPAAP
NLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTCRALDQWAFEHGVELRLIQPGKPTQNGF
IESFNGRFRDECLNEHWFSDVSHARKTISEWRQDYNECRPHSALNYQTPSEFAAAWRKGNSESEGSDITN
NLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTCRALDQWAFEHGVELRLIQPGKPTQNGF
IESFNGRFRDECLNEHWFSDVSHARKTISEWRQDYNECRPHSALNYQTPSEFAAAWRKGNSESEGSDITN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1041 bp | 346 aa | 72 | 1112 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRFSDQQIISILREAEAGVSARELCRKHAISDATFYTWRKKYGGMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKVLTTDQKREAVMLM
CDATGLSQRRACRLTGLSLSTCRYEAQRPAADAHLSGRIIELALERRRFGYRRIWQLLRRKGLATERLPLLRPAAPNLTWSMDFVMDALATGRRIKCLTC
VDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTCRALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDVSHA
RKTISEWRQDYNECRPHSALNYQTPSEFAAAWRKGNSESEGSDITN
CDATGLSQRRACRLTGLSLSTCRYEAQRPAADAHLSGRIIELALERRRFGYRRIWQLLRRKGLATERLPLLRPAAPNLTWSMDFVMDALATGRRIKCLTC
VDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTCRALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDVSHA
RKTISEWRQDYNECRPHSALNYQTPSEFAAAWRKGNSESEGSDITN
Blast result :
Comments
ISYen3 is 100% (ORFA) and 89% (ORFB) aa similar to IS1222. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Thomson,N.R., Howard,S., Wren,B.W., Holden,M.T., Crossman,L., Challis,G.L., Churcher,C., Mungall,K., Brooks,K., Chillingworth,T., Feltwell,T., Abdellah,Z., Hauser,H., Jagels,K., Maddison,M., Moule,S., Sanders,M., Whitehead,S., Quail,M.A., Dougan,G., Parkhill,J. and Prentice,M.B. (2006) (er) PLoS Genet. 2 (12), E206