ISRle4
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008382 | ND | Rhizobium leguminosarum | Rhizobium leguminosarum bv. viciae 3841 |
DNA section
IS Length : 1272 bp
Ends
IR Length : 33/46
IRL : TGACCTTGCCCCGTTGAAATAATCCATTTTGAAGTAAGCTCTGGCCCATT
IRR : TGACCTTGCCCCCAAGTTTTATCCAGTTTTGAGTTCGCTCCAGCGGTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGAAGC | CTGT | GGATCT | 4 |
DNA sequence
TGACCTTGCCCCGTTGAAATAATCCATTTTGAAGTAAGCTCTGGCCCATTGAGAGGACCAGAGGATGAAGCGCAATCGTTTCACGGACGAACAGATCATC
GGCATATTGAAGGAGCACGAGGCAGGCACGCCGGTCTCGGAGCTTTGCCGCAAGCATGGCGTCAGCGATGCTAGCATCTATAAATGGAAGGCCAAGTTCG
GCGGCATGGAGGTGTCCGAGGCCAAGCGGCTGAAGACGCTTGAGGACGAGAACACGAAGCTGAAACGGCTTCTGGCGGATGCGATGCTCGACAATGCTGC
CTTGAAAGACCTTTTGGGAAAGAAGTGGTGACGCCCGCGGCCAAGCGGAACGCTGTCGCGCATCTGATGAGCCATCATGAGATGAGCGAACGGCGGGCGT
GTAAAGCCATTGGTTTTTGCCGAATGACGGTCCGTTACGAGACCAGGCGCGACAATGATCATGAGCTTCGCGAGCGAATGAAGGCGTTGGCGCATGAACG
TCGCCGCTTCGGATATCGGCGCATTCATGTGCTGCTCCGGCGGGAGGGTCACCTCGTGAACCACAAGAGGCTCTTCCGGCTCTATCGGGAGGAGAAACTG
ACGGTGCGCAAGCGCGGCGGTCGCAAGCGAGCGATAGGCACGCGAGCGCCGATGCTGGTGCCGATGGTGGCCAATGATCGTTGGTCGCTGGACTTCGTGT
CGGATCAGTTCACCGATGGGCGCAGATTGCGGATTCTGACCGTCGTCGATGATTGCACGAGGGAGTGCCTGGCGCTCGTCGCCGATACATCGCTTTCCGG
TCTTCGCGTCGCACGGGAGCTTGACCGGATTATCGAGGAGCGCGGCAAGCCGAGGATGATCGTCAGCGACAACGGCAGCGAGTTCACCAGCAACGCGATC
CTGCAATGGGCGGACCGGACCAAGGTTGACTGGCATTACATTGCGCCTGGCAGGCCTATCCAGAACGCTTTTATCGAAAGCTTCAACGGGCGGCTGCGAG
ACGAGTTCTTGAATGAAACTCTGTTCTCGTCGCTTGCTCACGCCCGGTCTGCGCTTTCAAACTGGCGTAGCGATTACAACGATCAACGCCCTCATTCAGG
CCTTGGCTGGCTGACACCGGCCGAATTCGCTCAGACACTCAACCCGCGACGTGATGCGGTGCTGCGCAGCCGAAATGGCTCCGCACCGCAACCCGCCGCT
ACCGAACCAACAACAGCAACCAAAAACCGCTGGAGCGAACTCAAAACTGGATAAAACTTGGGGGCAAGGTCA
GGCATATTGAAGGAGCACGAGGCAGGCACGCCGGTCTCGGAGCTTTGCCGCAAGCATGGCGTCAGCGATGCTAGCATCTATAAATGGAAGGCCAAGTTCG
GCGGCATGGAGGTGTCCGAGGCCAAGCGGCTGAAGACGCTTGAGGACGAGAACACGAAGCTGAAACGGCTTCTGGCGGATGCGATGCTCGACAATGCTGC
CTTGAAAGACCTTTTGGGAAAGAAGTGGTGACGCCCGCGGCCAAGCGGAACGCTGTCGCGCATCTGATGAGCCATCATGAGATGAGCGAACGGCGGGCGT
GTAAAGCCATTGGTTTTTGCCGAATGACGGTCCGTTACGAGACCAGGCGCGACAATGATCATGAGCTTCGCGAGCGAATGAAGGCGTTGGCGCATGAACG
TCGCCGCTTCGGATATCGGCGCATTCATGTGCTGCTCCGGCGGGAGGGTCACCTCGTGAACCACAAGAGGCTCTTCCGGCTCTATCGGGAGGAGAAACTG
ACGGTGCGCAAGCGCGGCGGTCGCAAGCGAGCGATAGGCACGCGAGCGCCGATGCTGGTGCCGATGGTGGCCAATGATCGTTGGTCGCTGGACTTCGTGT
CGGATCAGTTCACCGATGGGCGCAGATTGCGGATTCTGACCGTCGTCGATGATTGCACGAGGGAGTGCCTGGCGCTCGTCGCCGATACATCGCTTTCCGG
TCTTCGCGTCGCACGGGAGCTTGACCGGATTATCGAGGAGCGCGGCAAGCCGAGGATGATCGTCAGCGACAACGGCAGCGAGTTCACCAGCAACGCGATC
CTGCAATGGGCGGACCGGACCAAGGTTGACTGGCATTACATTGCGCCTGGCAGGCCTATCCAGAACGCTTTTATCGAAAGCTTCAACGGGCGGCTGCGAG
ACGAGTTCTTGAATGAAACTCTGTTCTCGTCGCTTGCTCACGCCCGGTCTGCGCTTTCAAACTGGCGTAGCGATTACAACGATCAACGCCCTCATTCAGG
CCTTGGCTGGCTGACACCGGCCGAATTCGCTCAGACACTCAACCCGCGACGTGATGCGGTGCTGCGCAGCCGAAATGGCTCCGCACCGCAACCCGCCGCT
ACCGAACCAACAACAGCAACCAAAAACCGCTGGAGCGAACTCAAAACTGGATAAAACTTGGGGGCAAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 65 | 331 | + | No |
Description : First part of the transposase
ORF sequence :
MKRNRFTDEQIIGILKEHEAGTPVSELCRKHGVSDASIYKWKAKFGGMEVSEAKRLKTLEDENTKLKRLLADAMLDNAALKDLLGKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1011 bp | 336 aa | 244 | 1254 | + | No |
Description : Second part of the transposase
ORF sequence :
GREHEAETASGGCDARQCCLERPFGKEVVTPAAKRNAVAHLMSHHEMSERRACKAIGFCRMTVRYETRRDNDHELRERMKALAHERRRFGYRRIHVLLRR
EGHLVNHKRLFRLYREEKLTVRKRGGRKRAIGTRAPMLVPMVANDRWSLDFVSDQFTDGRRLRILTVVDDCTRECLALVADTSLSGLRVARELDRIIEER
GKPRMIVSDNGSEFTSNAILQWADRTKVDWHYIAPGRPIQNAFIESFNGRLRDEFLNETLFSSLAHARSALSNWRSDYNDQRPHSGLGWLTPAEFAQTLN
PRRDAVLRSRNGSAPQPAATEPTTATKNRWSELKTG
EGHLVNHKRLFRLYREEKLTVRKRGGRKRAIGTRAPMLVPMVANDRWSLDFVSDQFTDGRRLRILTVVDDCTRECLALVADTSLSGLRVARELDRIIEER
GKPRMIVSDNGSEFTSNAILQWADRTKVDWHYIAPGRPIQNAFIESFNGRLRDEFLNETLFSSLAHARSALSNWRSDYNDQRPHSGLGWLTPAEFAQTLN
PRRDAVLRSRNGSAPQPAATEPTTATKNRWSELKTG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 396 aa | 65 | 1254 | + | Yes |
Chemistry : DDE
ORF sequence :
MKRNRFTDEQIIGILKEHEAGTPVSELCRKHGVSDASIYKWKAKFGGMEVSEAKRLKTLEDENTKLKRLLADAMLDNAALKDLLGKEVVTPAAKRNAVAH
LMSHHEMSERRACKAIGFCRMTVRYETRRDNDHELRERMKALAHERRRFGYRRIHVLLRREGHLVNHKRLFRLYREEKLTVRKRGGRKRAIGTRAPMLVP
MVANDRWSLDFVSDQFTDGRRLRILTVVDDCTRECLALVADTSLSGLRVARELDRIIEERGKPRMIVSDNGSEFTSNAILQWADRTKVDWHYIAPGRPIQ
NAFIESFNGRLRDEFLNETLFSSLAHARSALSNWRSDYNDQRPHSGLGWLTPAEFAQTLNPRRDAVLRSRNGSAPQPAATEPTTATKNRWSELKTG
LMSHHEMSERRACKAIGFCRMTVRYETRRDNDHELRERMKALAHERRRFGYRRIHVLLRREGHLVNHKRLFRLYREEKLTVRKRGGRKRAIGTRAPMLVP
MVANDRWSLDFVSDQFTDGRRLRILTVVDDCTRECLALVADTSLSGLRVARELDRIIEERGKPRMIVSDNGSEFTSNAILQWADRTKVDWHYIAPGRPIQ
NAFIESFNGRLRDEFLNETLFSSLAHARSALSNWRSDYNDQRPHSGLGWLTPAEFAQTLNPRRDAVLRSRNGSAPQPAATEPTTATKNRWSELKTG
Blast result :
Comments
ISRle4 is 100% (ORFA) and 90% (ORFB) aa similar to ISBm2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Young,J.W., Crossman,L.C., Johnston,A.W.B., Thomson,N.R., Ghazoui,Z.F., Hull,K.H., Wexler,M., Curson,A.R.J., Todd,J.D., Poole,P.S., Mauchline,T.H., East,A.K., Quail,M.A., Churcher,C., Arrowsmith,C., Cherevach,A., Chillingworth,T., Clarke,K., Cronin,A., Davis,P., Fraser,A., Hance,Z., Hauser,H., Jagels,K., Moule,S., Mungall,K., Norbertczak,H., Rabbinowitsch,E., Sanders,M., Simmonds,M., Whitehead,S. and Parkhill,J. (2006) Genome Biol. 7, R4-R4