ISPa32
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002516 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa PAO1 |
DNA section
IS Length : 1236 bp
Ends
IR Length : 33/42
IRL : TGACCTGCTCCCCGATTTCAGTACCACGCCGAACTTAGTAGAGTCCGTTT
IRR : TGACCTGCCCCCAGTTTTAGTACCGCTGCCTAGTTAGTAGGCCCTGGGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTGAAGATA | CGAATCCTAC | 0 |
DNA sequence
TGACCTGCTCCCCGATTTCAGTACCACGCCGAACTTAGTAGAGTCCGTTTTCCGAGCAGAAGACGGCAGTGAAAAAGCGTTTTACCGAAGAGCAGATTCT
CGACTTCCTCAAGCAGGCGGAGGCCGGTGTACCGGTGAAGGAACTGTGTCGCCGACACGGCTTCAGTGATGCCTCGTTCTACACCTGGCGGGCCAAGTTT
GGCGGTATGACCGTGGCGGACGCCAAACGGTTGAAGGATCTTGAACTGGAAAATAGCCGATTGAAGAAGTTGCTCGCCGAGGCCCACCTCGACATCGAGT
CGCTGAAAGTGGTCGCTCGGGGAAAAGGGTAAGCCCGACAGCACGGCGGGAGGCGGTGCAGGAGATGCAGGCGCGAACCGGCATCTCCGAGCGTCGTGCC
TGTCAGTTGATCGGGCTGTCCCGCTCGGTGTTGCGCTACCAGCCGCGAGCCAGTGTGCAAAACACCGAGCTGCAAGCCCAACTGGTGGAACTGGCCCAAG
AGCGCCGGCGCTTTGGCTATCGCCGCCTGCACATCCTGCTGCGGCGTGCTGGCGTGCAGGTCAACCACAAGCGGATCTATCGCCTGTACCGCGCTGCCGG
CTTGATGGTGAAACGGCGGCGCCGCCGCCATGGCATCGCGGTGGAGCGCGAACGTCTGAGTTTGCCGAGCGCACCAAACCAGGTCTGGTCGATGGACTTC
GTCTTCGATGCACTGAGCACCGGGCGGCGGATCAAATGCCTGACGGTGGTCGATGACTTCACCAAGGAGTCGGTCGGCATCCTGGTGGAGCACGGTATCA
GCGGCTTTCGCGTCACACGGGCGCTGGACGAGATGGCGCGGTTTCGCGGCTACCCGAAAGCGATCCGCACCGACCAGGGGCCCGAGTTCACCGGCAAGGC
ACTCGATCAATGGGCCTATCAGCGCGACATCAAGCTGAAGCTGATTCAGCCTGGTAAGCCCACGCAGAATGCCTTCATCGAGTCGTTCAACGGTAAGTTC
CGGGACGAATGCCTCAATGAGCACTGGTTCTGTTCGCTGGCCGAAGCGAGAATCCGCACCGCGGCCTGGCGGCGGGATTACAACGAACACCGACCGCACA
GCGCGATTGGCAATCTCGCCCCGGCAGAATTTGCTGCAAGTTGGCGAACTCGCCAGCAGCAGCTGAAGCAGGAAAAATTAATATCAACCCCAGGGCCTAC
TAACTAGGCAGCGGTACTAAAACTGGGGGCAGGTCA
CGACTTCCTCAAGCAGGCGGAGGCCGGTGTACCGGTGAAGGAACTGTGTCGCCGACACGGCTTCAGTGATGCCTCGTTCTACACCTGGCGGGCCAAGTTT
GGCGGTATGACCGTGGCGGACGCCAAACGGTTGAAGGATCTTGAACTGGAAAATAGCCGATTGAAGAAGTTGCTCGCCGAGGCCCACCTCGACATCGAGT
CGCTGAAAGTGGTCGCTCGGGGAAAAGGGTAAGCCCGACAGCACGGCGGGAGGCGGTGCAGGAGATGCAGGCGCGAACCGGCATCTCCGAGCGTCGTGCC
TGTCAGTTGATCGGGCTGTCCCGCTCGGTGTTGCGCTACCAGCCGCGAGCCAGTGTGCAAAACACCGAGCTGCAAGCCCAACTGGTGGAACTGGCCCAAG
AGCGCCGGCGCTTTGGCTATCGCCGCCTGCACATCCTGCTGCGGCGTGCTGGCGTGCAGGTCAACCACAAGCGGATCTATCGCCTGTACCGCGCTGCCGG
CTTGATGGTGAAACGGCGGCGCCGCCGCCATGGCATCGCGGTGGAGCGCGAACGTCTGAGTTTGCCGAGCGCACCAAACCAGGTCTGGTCGATGGACTTC
GTCTTCGATGCACTGAGCACCGGGCGGCGGATCAAATGCCTGACGGTGGTCGATGACTTCACCAAGGAGTCGGTCGGCATCCTGGTGGAGCACGGTATCA
GCGGCTTTCGCGTCACACGGGCGCTGGACGAGATGGCGCGGTTTCGCGGCTACCCGAAAGCGATCCGCACCGACCAGGGGCCCGAGTTCACCGGCAAGGC
ACTCGATCAATGGGCCTATCAGCGCGACATCAAGCTGAAGCTGATTCAGCCTGGTAAGCCCACGCAGAATGCCTTCATCGAGTCGTTCAACGGTAAGTTC
CGGGACGAATGCCTCAATGAGCACTGGTTCTGTTCGCTGGCCGAAGCGAGAATCCGCACCGCGGCCTGGCGGCGGGATTACAACGAACACCGACCGCACA
GCGCGATTGGCAATCTCGCCCCGGCAGAATTTGCTGCAAGTTGGCGAACTCGCCAGCAGCAGCTGAAGCAGGAAAAATTAATATCAACCCCAGGGCCTAC
TAACTAGGCAGCGGTACTAAAACTGGGGGCAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 263 bp | 87 aa | 70 | 332 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRFTEEQILDFLKQAEAGVPVKELCRRHGFSDASFYTWRAKFGGMTVADAKRLKDLELENSRLKKLLAEAHLDIESLKVVARGKG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 257 | 1207 | + | No |
Description : Second part of the transposase
ORF sequence :
PIEEVARRGPPRHRVAESGRSGKRVSPTARREAVQEMQARTGISERRACQLIGLSRSVLRYQPRASVQNTELQAQLVELAQERRRFGYRRLHILLRRAGV
QVNHKRIYRLYRAAGLMVKRRRRRHGIAVERERLSLPSAPNQVWSMDFVFDALSTGRRIKCLTVVDDFTKESVGILVEHGISGFRVTRALDEMARFRGYP
KAIRTDQGPEFTGKALDQWAYQRDIKLKLIQPGKPTQNAFIESFNGKFRDECLNEHWFCSLAEARIRTAAWRRDYNEHRPHSAIGNLAPAEFAASWRTRQ
QQLKQEKLISTPGPTN
QVNHKRIYRLYRAAGLMVKRRRRRHGIAVERERLSLPSAPNQVWSMDFVFDALSTGRRIKCLTVVDDFTKESVGILVEHGISGFRVTRALDEMARFRGYP
KAIRTDQGPEFTGKALDQWAYQRDIKLKLIQPGKPTQNAFIESFNGKFRDECLNEHWFCSLAEARIRTAAWRRDYNEHRPHSAIGNLAPAEFAASWRTRQ
QQLKQEKLISTPGPTN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1138 bp | 379 aa | 70 | 1207 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRFTEEQILDFLKQAEAGVPVKELCRRHGFSDASFYTWRAKFGGMTVADAKRLKDLELENSRLKKLLAEAHLDIESLKVVARGKRVSPTARREAVQEM
QARTGISERRACQLIGLSRSVLRYQPRASVQNTELQAQLVELAQERRRFGYRRLHILLRRAGVQVNHKRIYRLYRAAGLMVKRRRRRHGIAVERERLSLP
SAPNQVWSMDFVFDALSTGRRIKCLTVVDDFTKESVGILVEHGISGFRVTRALDEMARFRGYPKAIRTDQGPEFTGKALDQWAYQRDIKLKLIQPGKPTQ
NAFIESFNGKFRDECLNEHWFCSLAEARIRTAAWRRDYNEHRPHSAIGNLAPAEFAASWRTRQQQLKQEKLISTPGPTN
QARTGISERRACQLIGLSRSVLRYQPRASVQNTELQAQLVELAQERRRFGYRRLHILLRRAGVQVNHKRIYRLYRAAGLMVKRRRRRHGIAVERERLSLP
SAPNQVWSMDFVFDALSTGRRIKCLTVVDDFTKESVGILVEHGISGFRVTRALDEMARFRGYPKAIRTDQGPEFTGKALDQWAYQRDIKLKLIQPGKPTQ
NAFIESFNGKFRDECLNEHWFCSLAEARIRTAAWRRDYNEHRPHSAIGNLAPAEFAASWRTRQQQLKQEKLISTPGPTN
Blast result :
Comments
ISPa32 is 80% (ORFA) and 95% (ORFB) aa similar to IS407. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Winsor,G.L., Lo,R., Sui,S.J., Ung,K.S., Huang,S., Cheng,D., Ching,W.K., Hancock,R.E. and Brinkman,F.S. (2005) Nucleic Acids Res. 33 (DATABASE ISSUE), D338-D343.