ISPae1

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AAIJ01000014 ND Prosthecochloris aestuarii
Prosthecochloris aestuarii DSM 271
DNA section
IS Length : 1196 bp

Ends


IR Length : 28/42

IRL : GGACGTGAGCCCGAAAAACCGGACCACTTGGTTGTAGAAAAAAAGCTTAA
IRR : GGACGTGCCCCCGATCAATAGACCACTTCTTTTTGGCAAAATTCAGTTTG

Insertion site


Left flankDirect repeatRight flankDR Length
GATTTAACGAGTTGTTATACATTT4

DNA sequence

GGACGTGAGCCCGAAAAACCGGACCACTTGGTTGTAGAAAAAAAGCTTAAACTCAACCAAGGAGGAATAGGTCATGAAAACCAAGCGTTACAGCACGGAA
CAGATCATCGGGATCCTGAAAGAAGCCGAGGCAGGCATGCCGGTCAAGGAGCTCTGCCGCAAGTACGGCGTCAGCGATGCGACGATTTACAACTGGAAAT
CGAAGTATGCCGGCATGAGCGTCAGCGAGGCGCAGCGTCTCAAGGAGCTTGAAAGCGAGAACCATCGTTTGAAGAAGCTGGTTGCCGATCAGGCGCTCGA
TATCCAGATGCTCAAGGAGATCAATTCAAAAAAGTGGTAACGCCCGAGGCGAAGCGCAATGCCGTCAAGCATTTGCACGATACCTTCGGGCAAAGTCTGA
GAAAGCTCTGTATCTTGATCGGGCTGAACCGTTCAAGCTGGTATTACGAGCCTCAACCGGATAGCAACGAACCAATCAGGAAGCGGTTACGGGAATTGGC
CGATGAGCGCAAACGCTGGGGGTACCGGCGATTGCATTACCTGTTGCGTCGAGAAGGATTTCAGATCAACCACAAACGGACAGAACGGCTGTACCGGGAA
GAAAACCTGATGCTGCGGGTCAGAAGGCGCAGGAAGATGGCCTCAGAGAACCGTGTTGCCCCGCCACCACCGGAGCGGAAGAACCACTGCTGGGCGATGG
ATTTTATGAGCGACAATCTGTATAACGGCCGTCGTTTTCGGGTACTCAACGTGCTGGACAGCTACAGCAGGGATTATCTCGGTTTTGAAGTCGATACCTC
AATCAATGGCAAGCGAGTATGCAGTGTTCTCGAACGGATTGCCTGGTTCAAAGGCTTGCCGGAACTGATCACGGTGGACAATGGCCCAGAATTCATCGGT
AAGGCACTGGATGCCTGGGCACATCGGCATGGAGTGAAGCTGGTGTTCAATCGACCGGGCAAGCCGGTTGACAACACCTATATCGAAAGCTTTAACGGCA
GGCTGCGGGATGAATGCCTGAACGTAAACTGGTTTATGAGTCTGGGGCATGCACGAGAGGTTATCGCAGAATGGCAGGAGGATTACAATTCGGTCCGTCC
GCACAGCTCATTGGGAACGCGGACACCCGAAGAATTTCTGGTTCAGCAAACTGAATTTTGCCAAAAAGAAGTGGTCTATTGATCGGGGGCACGTCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa74340+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKTKRYSTEQIIGILKEAEAGMPVKELCRKYGVSDATIYNWKSKYAGMSVSEAQRLKELESENHRLKKLVADQALDIQMLKEINSKKW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
930 bp309 aa2531182+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KREPSFEEAGCRSGARYPDAQGDQFKKVVTPEAKRNAVKHLHDTFGQSLRKLCILIGLNRSSWYYEPQPDSNEPIRKRLRELADERKRWGYRRLHYLLRR
EGFQINHKRTERLYREENLMLRVRRRRKMASENRVAPPPPERKNHCWAMDFMSDNLYNGRRFRVLNVLDSYSRDYLGFEVDTSINGKRVCSVLERIAWFK
GLPELITVDNGPEFIGKALDAWAHRHGVKLVFNRPGKPVDNTYIESFNGRLRDECLNVNWFMSLGHAREVIAEWQEDYNSVRPHSSLGTRTPEEFLVQQT
EFCQKEVVY

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1109 bp369 aa741182+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKTKRYSTEQIIGILKEAEAGMPVKELCRKYGVSDATIYNWKSKYAGMSVSEAQRLKELESENHRLKKLVADQALDIQMLKEINSKKVVTPEAKRNAVKH
LHDTFGQSLRKLCILIGLNRSSWYYEPQPDSNEPIRKRLRELADERKRWGYRRLHYLLRREGFQINHKRTERLYREENLMLRVRRRRKMASENRVAPPPP
ERKNHCWAMDFMSDNLYNGRRFRVLNVLDSYSRDYLGFEVDTSINGKRVCSVLERIAWFKGLPELITVDNGPEFIGKALDAWAHRHGVKLVFNRPGKPVD
NTYIESFNGRLRDECLNVNWFMSLGHAREVIAEWQEDYNSVRPHSSLGTRTPEEFLVQQTEFCQKEVVY

 

Blast result :
Comments
Genome in progress.
ISPae1 is 83% (ORFA) and 66% (ORFB) aa similar to IS407. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Direct submission Genbank.