ISCte3
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAUJ01000003 | ND | Comamonas testosteroni | Comamonas testosteroni KF-1 |
DNA section
IS Length : 1203 bp
Ends
IR Length : 36/43
IRL : CGACGTCCCCCCGCCTTGAGTAGCACTTGAGCTTGGAGTCCAATCCCAAC
IRR : CGACGTCCCCCCGTATTTAAGTAGCACTCGGCTTTAGAGTCCGAGGGTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGCGCGTCT | CGGGC | ATCAAACCCG | 5 |
DNA sequence
CGACGTCCCCCCGCCTTGAGTAGCACTTGAGCTTGGAGTCCAATCCCAACGACAGGAGATTGGATATGAAGAAGCGATTCACAGAAGAGCAGATCATTGG
CTTCCTGCGGGAAGCTGAATCAGGCTTGCCGGTAGCTGAGCTTTGCCGCAGGCACGGATTCTCGGAGGCCAGTTACTACCTTTGGCGCAGCAAGTTTGGA
GGCATGAACGTGTCCGATGCCAAACGCCTCAAGGAGCTGGAAACCGAGAACGGGCGACTTAAACGGCTGTTGGCCGATGCACTGCTTGAAAACGAGGTGA
CGAAAGAAGCCTTGAGAAAAAAGTGGTGAGCGCACCTGCAAGGCGGGAGCTGGTGCGCCACATGAACTGCCAAGGGCTGAGTGAGCGACGTGCACTGCGT
GTCATTGGAATGAGCGCCAGTGCTTACCGCTACCAACCAGCCGTAGATCGCAACTGCGACTTGCGACACAAGATTATTGCTTTAGCTCAGCGTCACCGGC
GTTACGGCTCAGGCATGATCTATCTGAAGCTGCGCCAAGCTGGCGAGCTGGTCAATCACAAGCGGGTTGAGCGTCTGTATGCCCAAGCTGGTCTGCAAGT
GAAGAGGCGCAAGCGTAAAAAGATCCCGCTGTCCGAGCGACACCCTCTAGAGCGCCCAACGCTAGCCAATCAAGTGTGGTCTATGGACTTTGTGTTTGAC
CGAACAGCCGAAGGACGCTGCATCAAGAACTTGACCGTGATTGATGACGCCACGCATGAAGCGGTAGCGATCGTGTGCGAGCGTGCATTGGGTGGCCACC
AATTGGTGCGTATTCTGGAACAACTGGCAAGGACACGGGGCTTGCCCAAAGCCATCAGAACAGACAACGGTAAGGAGTTCTGCAGCCGTGCAATGGTGAG
CTGGGCGCATGCACGAGGCGTGCAGTTGTTCTTGATCGAACCGGGTAAGCCCAACCAGAACGCTTATATCGAATCATTCAACGGGCGGTTCCGCGATGAA
TGTCTCAATGAGCACTGGTTCACCAGCCTGGCACATGCTCGGGTCGTCGTCGAAACTTGGAAACGGGAATACAACGAGGAAAGACCTAAGAAAGCACTTG
GCGGGCTTACGCCCGCTGCCTATGCGCAGGCCATGACACAGAAACAGTTAAATTAACCCTCGGACTCTAAAGCCGAGTGCTACTTAAATACGGGGGGACG
TCG
CTTCCTGCGGGAAGCTGAATCAGGCTTGCCGGTAGCTGAGCTTTGCCGCAGGCACGGATTCTCGGAGGCCAGTTACTACCTTTGGCGCAGCAAGTTTGGA
GGCATGAACGTGTCCGATGCCAAACGCCTCAAGGAGCTGGAAACCGAGAACGGGCGACTTAAACGGCTGTTGGCCGATGCACTGCTTGAAAACGAGGTGA
CGAAAGAAGCCTTGAGAAAAAAGTGGTGAGCGCACCTGCAAGGCGGGAGCTGGTGCGCCACATGAACTGCCAAGGGCTGAGTGAGCGACGTGCACTGCGT
GTCATTGGAATGAGCGCCAGTGCTTACCGCTACCAACCAGCCGTAGATCGCAACTGCGACTTGCGACACAAGATTATTGCTTTAGCTCAGCGTCACCGGC
GTTACGGCTCAGGCATGATCTATCTGAAGCTGCGCCAAGCTGGCGAGCTGGTCAATCACAAGCGGGTTGAGCGTCTGTATGCCCAAGCTGGTCTGCAAGT
GAAGAGGCGCAAGCGTAAAAAGATCCCGCTGTCCGAGCGACACCCTCTAGAGCGCCCAACGCTAGCCAATCAAGTGTGGTCTATGGACTTTGTGTTTGAC
CGAACAGCCGAAGGACGCTGCATCAAGAACTTGACCGTGATTGATGACGCCACGCATGAAGCGGTAGCGATCGTGTGCGAGCGTGCATTGGGTGGCCACC
AATTGGTGCGTATTCTGGAACAACTGGCAAGGACACGGGGCTTGCCCAAAGCCATCAGAACAGACAACGGTAAGGAGTTCTGCAGCCGTGCAATGGTGAG
CTGGGCGCATGCACGAGGCGTGCAGTTGTTCTTGATCGAACCGGGTAAGCCCAACCAGAACGCTTATATCGAATCATTCAACGGGCGGTTCCGCGATGAA
TGTCTCAATGAGCACTGGTTCACCAGCCTGGCACATGCTCGGGTCGTCGTCGAAACTTGGAAACGGGAATACAACGAGGAAAGACCTAAGAAAGCACTTG
GCGGGCTTACGCCCGCTGCCTATGCGCAGGCCATGACACAGAAACAGTTAAATTAACCCTCGGACTCTAAAGCCGAGTGCTACTTAAATACGGGGGGACG
TCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 274 bp | 87 aa | 66 | 339 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRFTEEQIIGFLREAESGLPVAELCRRHGFSEASYYLWRSKFGGMNVSDAKRLKELETENGRLKRLLADALLENEVTKEALRKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 867 bp | 288 aa | 290 | 1156 | + | No |
Description : Second part of the transposase
ORF sequence :
KRGDERSLEKKVVSAPARRELVRHMNCQGLSERRALRVIGMSASAYRYQPAVDRNCDLRHKIIALAQRHRRYGSGMIYLKLRQAGELVNHKRVERLYAQA
GLQVKRRKRKKIPLSERHPLERPTLANQVWSMDFVFDRTAEGRCIKNLTVIDDATHEAVAIVCERALGGHQLVRILEQLARTRGLPKAIRTDNGKEFCSR
AMVSWAHARGVQLFLIEPGKPNQNAYIESFNGRFRDECLNEHWFTSLAHARVVVETWKREYNEERPKKALGGLTPAAYAQAMTQKQLN
GLQVKRRKRKKIPLSERHPLERPTLANQVWSMDFVFDRTAEGRCIKNLTVIDDATHEAVAIVCERALGGHQLVRILEQLARTRGLPKAIRTDNGKEFCSR
AMVSWAHARGVQLFLIEPGKPNQNAYIESFNGRFRDECLNEHWFTSLAHARVVVETWKREYNEERPKKALGGLTPAAYAQAMTQKQLN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1091 bp | 362 aa | 66 | 1156 | + | Yes |
Chemistry : DDE
ORF sequence :
KKRFTEEQIIGFLREAESGLPVAELCRRHGFSEASYYLWRSKFGGMNVSDAKRLKELETENGRLKRLLADALLENEVTKEALRKKVVSAPARRELVRHMN
CQGLSERRALRVIGMSASAYRYQPAVDRNCDLRHKIIALAQRHRRYGSGMIYLKLRQAGELVNHKRVERLYAQAGLQVKRRKRKKIPLSERHPLERPTLA
NQVWSMDFVFDRTAEGRCIKNLTVIDDATHEAVAIVCERALGGHQLVRILEQLARTRGLPKAIRTDNGKEFCSRAMVSWAHARGVQLFLIEPGKPNQNAY
IESFNGRFRDECLNEHWFTSLAHARVVVETWKREYNEERPKKALGGLTPAAYAQAMTQKQLN
CQGLSERRALRVIGMSASAYRYQPAVDRNCDLRHKIIALAQRHRRYGSGMIYLKLRQAGELVNHKRVERLYAQAGLQVKRRKRKKIPLSERHPLERPTLA
NQVWSMDFVFDRTAEGRCIKNLTVIDDATHEAVAIVCERALGGHQLVRILEQLARTRGLPKAIRTDNGKEFCSRAMVSWAHARGVQLFLIEPGKPNQNAY
IESFNGRFRDECLNEHWFTSLAHARVVVETWKREYNEERPKKALGGLTPAAYAQAMTQKQLN
Blast result :
Comments
Genome in progress.
ISCte3 is 91 % (ORFA) aa similar to IS1404 and 81% (ORFB) aa similar to IS1389-B. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift. The terminal inverted repeat are not expected ends for an IS3 family members.
ISCte3 is 91 % (ORFA) aa similar to IS1404 and 81% (ORFB) aa similar to IS1389-B. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift. The terminal inverted repeat are not expected ends for an IS3 family members.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P.(2006) Direct Submission GenBank.