ISSag5

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007432 ND Streptococcus agalactiae
Streptococcus agalactiae A909
DNA section
IS Length : 1259 bp

Ends


IR Length : 23/36

IRL : TGGATGTATACTATAAAAGTAGACAAAACTTTGTGTGTTACAATCTGTTT
IRR : TGGACTAATGTCAAATAAGTAGACAGAAAACCGTGTTATTTTATTGCGTT

Insertion site


Left flankDirect repeatRight flankDR Length
TGCCAAAGGGCTCTGTGAAT0

DNA sequence

TGGATGTATACTATAAAAGTAGACAAAACTTTGTGTGTTACAATCTGTTTTAAAAGACACAAAAACGAAAGGACAACCTATGTCAACATTTAAACGCTAT
GATGAAGAATTCAAACAATCTCTTGTTAACCTTTATCAAACTGGAAAAACTCAGTCTGAACTCTGTAAAGACTATAGGGTATCCACTTCTGCGCTTGCAA
AATGGATCAAACAGTACTCTCAATTCAAACTCGAAGATAATTCTGTACTTACTGCCAAGCAAATACAAGAGTTACAAAAACGTAATGCGCAGCTTGAAGA
GGAGAACCTTATCCTAAAAAAAGCAAGTGCCATATTCATGCAAAACTTAAAGTAAGACTCCTCGCTGTCTATCGGTTACGCTTTGAACACGCCACAACAA
CCTTGTGTCGTGTTTTACGTGTCAATCGCTCCACTTACTATAAATTTCTAAAACATAAGCCCTCAAAAAGAGAATTGGATAATCAAATTTATAAAAAACA
AATACTTGAGATTTATACCAAAGCAAACAAAAGACTTGGTGTGAAGTCTATCAAAGTCATTCTTCAAAGAGACTACGACACAAAAATCTCTGAAGGAAGA
ATTTACCATCTGATGAAGAATATGGCGCTCCCTAAAATGGCTACCGTTAAGACAAAAACAGCTCTTAAAAAGACTCAAAAAACGTATCCTCAAAACTTAC
TCAACCAGAAATTTTATCCTGACAAACCTAATCAAGTATGGTCTACTGACTTCACCTATATTTCTATTGGATATAAGAAATATGTCTATCTCTGCGCAAT
ACTTGATCTCTATTCTAGAAAATGTATTGCTTGGAAATTGAGTCACCGTATGGATGCAAAGTTAGCATGTAACACTCTAGAATTAGCTCTTAATAAAAGA
AAGATTGAAGGAACACTTCTCTTTCATTCCGACCAAGGGTCACAATTTAAGGCCAGGGAATTTAGAAAAATAATTGATGACAACAATATCATGCATTCTT
TTTCTAAACCTGGATATCCTTATGATAATGCCGTAACTGAAGCATTTTTCAAGTATTTAAAGCATAGACAAATCAACCGAAAACATTATCAAAATATCAA
ACAGGTTCAATTAGACTGCTTTGAATACATTGAGAATTTTTATAACAATTACATCCCACATACGGCTAATCTAGGACTAACCCCTAATCAGAAAGAAGAA
AATTATTTTAACGCAATAAAATAACACGGTTTTCTGTCTACTTATTTGACATTAGTCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
276 bp91 aa80355+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIKQYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLILKKASAIFMQNLK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
927 bp308 aa2981224+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RGEPYPKKSKCHIHAKLKVRLLAVYRLRFEHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKVILQRDYDTKISEG
RIYHLMKNMALPKMATVKTKTALKKTQKTYPQNLLNQKFYPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNK
RKIEGTLLFHSDQGSQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKHYQNIKQVQLDCFEYIENFYNNYIPHTANLGLTPNQKE
ENYFNAIK

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1145 bp381 aa801224+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIKQYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLILKKSKCHIHAKLKVRLLAVYRL
RFEHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKVILQRDYDTKISEGRIYHLMKNMALPKMATVKTKTALKKTQ
KTYPQNLLNQKFYPDKPNQVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNKRKIEGTLLFHSDQGSQFKAREFRKIID
DNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINRKHYQNIKQVQLDCFEYIENFYNNYIPHTANLGLTPNQKEENYFNAIK

 

Blast result :
Comments
There is 1 copy of ISSag5 in the chromosome of Streptococcus agalactiae A909. This transposase gene is related to but different from ISSag4 transposase.
ISSag5 is 78% (ORFA) and 82% (ORFB) aa similar to IS-LL6 . The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Tettelin,H., Masignani,V., Cieslewicz,M.J., Donati,C., Medini,D., Ward,N.L., Angiuoli,S.V., Crabtree,J., Jones,A.L., Durkin,A.S., Deboy,R.T., Davidsen,T.M., Mora,M., Scarselli,M., Margarit Y Ros,I., Peterson,J.D., Hauser,C.R., Sundaram,J.P., Nelson,W.C., Madupu,R., Brinkac,L.M., Dodson,R.J., Rosovitz,M.J., Sullivan,S.A., Daugherty,S.C., Haft,D.H., Selengut,J., Gwinn,M.L., Zhou,L., Zafar,N., Khouri,H., Radune,D., Dimitrov,G., Watkins,K., O'connor,K.J., Smith,S., Utterback,T.R., White,O., Rubens,C.E., Grandi,G., Madoff,L.C., Kasper,D.L., Telford,J.L., Wessels,M.R., Rappuoli,R. and Fraser,C.M. (2005) Proc. Natl. Acad. Sci. U.S.A. 102 (39), 13950-13955
2] Tettelin,H., Masignani,V., Cieslewicz,M.J., Eisen,J.A.,Peterson,S., Wessels,M.R., Paulsen,I.T., Nelson,K.E., Margarit,I.,Read,T.D., Madoff,L.C., Wolf,A.M., Beanan,M.J., Brinkac,L.M., Daugherty,S.C., DeBoy,R.T., Durkin,S., Kolonay,J.F., Umayam,L.A., Madupu,R., Lewis,M.R., Radune,D., Fedorova,N.B., Scanlan,D., Khouri,H., Mulligan,S., Carty,H.A., Cline,R.T., Gill,J., Scarselli,M., Mora,M., Iacobini,E.T., Brettoni,C., Galli,G., Mariani,M., Vegni,F., Maione,D., Rinaudo,D., Rappuoli,R., Telford,J.L., Kasper,D.L., Grandi,G. and Fraser,C.M. (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (19), 12391-12396