ISSusp1
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AALV01000003.1 | ND | Sulfitobacter sp. | Sulfitobacter sp. EE-36 |
DNA section
IS Length : 1228 bp
Ends
IR Length : 24/32
IRL : TGAATAGCCCCTAGATTTGTAGACGCTTTCTTCCCTAACTTTGAGGCAAG
IRR : TGAATAGCCCCTAGTTCCCTAGACACCTTCTTGGTTCACGTTGAACTGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTGAACGAT | TGCCAAGATC | 0 |
DNA sequence
TGAATAGCCCCTAGATTTGTAGACGCTTTCTTCCCTAACTTTGAGGCAAGGAGGCCGTTATGGGGAAGCCAAATTTCAGTGATGATTTCAAGCGCGATGC
AGTCCATCAGATAACAGTGCGGGGGTATGCCGTTCGCGAGGTTTCGGAACGGTTGGGCGTCAGTACATATTCGCTCTACAAATGGATGAAGCTCTACGCA
AAAGCTGGGTCACAGACATCATCTGTTGACCATGAAGCTGAGAACCGCAGGCTCAAGCGTGAGTTGGCGCGCATGACGGAGGAGCGCGACATCCTAAAAA
AAGCGGCCGCGTACTTCGCAAAGGATGCAAGGTGAAGTACGCGTTCATTGCCGAGCACCGCCCTCAGTATTCGGTACGCAGGATGTGTCGTTGCCTCAAT
ATCCATCCCAGCGGGTTCTATGCATGGATTAAAAATCCATTAAGCCACCGTGCGCGTGAGGATGCGCGTCAGATCAAGCTGCTCGAAGAAGCTTGGAACG
ACAGTGGCAAGGTCTACGGATACCGTAAGCTGCATGACGATCTGGTGGAGCTGGGTGAGACCAGTTGCCCAAACCGTGTTGCTAGGTTGGCCCGTTTGGC
TGGGATCCGCGCAGAGATCGGGTACAAACGTAAGCCGGGCAAGTATGGTGGACGGCCGTCAGTCGTCGTCGACAACACGTTAGACCGGCAGTTTGATGTG
ACAGCGCCAGACACCGCCTGGGTGTCCGATATCACTTACATCAAAACCTATGAGGGGTTTCTCTATCTGGCGGTTATCATCGACCTCTACTCACGACGTG
TGATCGGCTGGGCGACACAATCGCGGCAACCAACAGACCTCGTGCTGCAAGCCCTTTTGATGGCTGTCTGGCGACGCAAACCTAAGCGACGCGTCCTGAT
CCACACGGATCAAGGCAGCCAATACACCAGCAGGGACTGGGCGGCGTTTCTGCGGGCCCACAATATCGAACACTCGATGAGCAGGCGCGGGAATTGCCAT
GACAATGCGGTCGCTGAGAGCTTCTTCAACCTGCTCAAACGGGAACGCGTCCGGCGTCGAACATATAAGACCCGCGAGCAGGCCAGACAGGACATCTTCG
ACTACATCGAAATGTTCTATAACCCGAAACGCAAACATGTCAGAAACGGTATGCTGTCGCCTGTCGACTTCGAAAACAGACAGTTCAACGTGAACCAAGA
AGGTGTCTAGGGAACTAGGGGCTATTCA
AGTCCATCAGATAACAGTGCGGGGGTATGCCGTTCGCGAGGTTTCGGAACGGTTGGGCGTCAGTACATATTCGCTCTACAAATGGATGAAGCTCTACGCA
AAAGCTGGGTCACAGACATCATCTGTTGACCATGAAGCTGAGAACCGCAGGCTCAAGCGTGAGTTGGCGCGCATGACGGAGGAGCGCGACATCCTAAAAA
AAGCGGCCGCGTACTTCGCAAAGGATGCAAGGTGAAGTACGCGTTCATTGCCGAGCACCGCCCTCAGTATTCGGTACGCAGGATGTGTCGTTGCCTCAAT
ATCCATCCCAGCGGGTTCTATGCATGGATTAAAAATCCATTAAGCCACCGTGCGCGTGAGGATGCGCGTCAGATCAAGCTGCTCGAAGAAGCTTGGAACG
ACAGTGGCAAGGTCTACGGATACCGTAAGCTGCATGACGATCTGGTGGAGCTGGGTGAGACCAGTTGCCCAAACCGTGTTGCTAGGTTGGCCCGTTTGGC
TGGGATCCGCGCAGAGATCGGGTACAAACGTAAGCCGGGCAAGTATGGTGGACGGCCGTCAGTCGTCGTCGACAACACGTTAGACCGGCAGTTTGATGTG
ACAGCGCCAGACACCGCCTGGGTGTCCGATATCACTTACATCAAAACCTATGAGGGGTTTCTCTATCTGGCGGTTATCATCGACCTCTACTCACGACGTG
TGATCGGCTGGGCGACACAATCGCGGCAACCAACAGACCTCGTGCTGCAAGCCCTTTTGATGGCTGTCTGGCGACGCAAACCTAAGCGACGCGTCCTGAT
CCACACGGATCAAGGCAGCCAATACACCAGCAGGGACTGGGCGGCGTTTCTGCGGGCCCACAATATCGAACACTCGATGAGCAGGCGCGGGAATTGCCAT
GACAATGCGGTCGCTGAGAGCTTCTTCAACCTGCTCAAACGGGAACGCGTCCGGCGTCGAACATATAAGACCCGCGAGCAGGCCAGACAGGACATCTTCG
ACTACATCGAAATGTTCTATAACCCGAAACGCAAACATGTCAGAAACGGTATGCTGTCGCCTGTCGACTTCGAAAACAGACAGTTCAACGTGAACCAAGA
AGGTGTCTAGGGAACTAGGGGCTATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 60 | 335 | + | No |
Description : First part of the transposase
ORF sequence :
MGKPNFSDDFKRDAVHQITVRGYAVREVSERLGVSTYSLYKWMKLYAKAGSQTSSVDHEAENRRLKRELARMTEERDILKKAAAYFAKDAR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 315 aa | 260 | 1210 | + | No |
Description : Second part of the transposase
ORF sequence :
VGAHDGGARHPKKSGRVLRKGCKVKYAFIAEHRPQYSVRRMCRCLNIHPSGFYAWIKNPLSHRAREDARQIKLLEEAWNDSGKVYGYRKLHDDLVELGET
SCPNRVARLARLAGIRAEIGYKRKPGKYGGRPSVVVDNTLDRQFDVTAPDTAWVSDITYIKTYEGFLYLAVIIDLYSRRVIGWATQSRQPTDLVLQALLM
AVWRRKPKRRVLIHTDQGSQYTSRDWAAFLRAHNIEHSMSRRGNCHDNAVAESFFNLLKRERVRRRTYKTREQARQDIFDYIEMFYNPKRKHVRNGMLSP
VDFENRQFNVNQEGV
SCPNRVARLARLAGIRAEIGYKRKPGKYGGRPSVVVDNTLDRQFDVTAPDTAWVSDITYIKTYEGFLYLAVIIDLYSRRVIGWATQSRQPTDLVLQALLM
AVWRRKPKRRVLIHTDQGSQYTSRDWAAFLRAHNIEHSMSRRGNCHDNAVAESFFNLLKRERVRRRTYKTREQARQDIFDYIEMFYNPKRKHVRNGMLSP
VDFENRQFNVNQEGV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 60 | 1210 | + | Yes |
Chemistry : DDE
ORF sequence :
MGKPNFSDDFKRDAVHQITVRGYAVREVSERLGVSTYSLYKWMKLYAKAGSQTSSVDHEAENRRLKRELARMTEERDILKKSGRVLRKGCKVKYAFIAEH
RPQYSVRRMCRCLNIHPSGFYAWIKNPLSHRAREDARQIKLLEEAWNDSGKVYGYRKLHDDLVELGETSCPNRVARLARLAGIRAEIGYKRKPGKYGGRP
SVVVDNTLDRQFDVTAPDTAWVSDITYIKTYEGFLYLAVIIDLYSRRVIGWATQSRQPTDLVLQALLMAVWRRKPKRRVLIHTDQGSQYTSRDWAAFLRA
HNIEHSMSRRGNCHDNAVAESFFNLLKRERVRRRTYKTREQARQDIFDYIEMFYNPKRKHVRNGMLSPVDFENRQFNVNQEGV
RPQYSVRRMCRCLNIHPSGFYAWIKNPLSHRAREDARQIKLLEEAWNDSGKVYGYRKLHDDLVELGETSCPNRVARLARLAGIRAEIGYKRKPGKYGGRP
SVVVDNTLDRQFDVTAPDTAWVSDITYIKTYEGFLYLAVIIDLYSRRVIGWATQSRQPTDLVLQALLMAVWRRKPKRRVLIHTDQGSQYTSRDWAAFLRA
HNIEHSMSRRGNCHDNAVAESFFNLLKRERVRRRTYKTREQARQDIFDYIEMFYNPKRKHVRNGMLSPVDFENRQFNVNQEGV
Blast result :
Comments
Genome in progress.
ISSusp1 is 74 % (ORFA) aa similar to ISXac3 and 78% (ORFB) aa similar to ISPsy29. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSusp1 is 74 % (ORFA) aa similar to ISXac3 and 78% (ORFB) aa similar to ISPsy29. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Moran,M.A., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A.,Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. andVenter,J.C. (2005) Direct submission GenBank.