ISSpr1
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAUN010 | ND | Serratia proteamaculans | Serratia proteamaculans 568 |
DNA section
IS Length : 1234 bp
Ends
IR Length : 31/39
IRL : TGAATAGACCCGGGTTTTATAGACACTTTTTTGCCTCATAATAGAGGCTT
IRR : TGAATAGACCCGGATTTCCTAGACGGTTCCGTGCCTCATGAACCAGGCAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCAAGGTCT | CCAT | CATGGATCTC | 4 |
DNA sequence
TGAATAGACCCGGGTTTTATAGACACTTTTTTGCCTCATAATAGAGGCTTAATGAAGGAGTGTTTATGTCAGCCAAAAATTTCACTGAAGAATTCAAAAT
TGAAGCCGTAAAGCAGGTCACTGAGCAGGGATATCCCGTATCCGAGGTCGCCTCCCGACTTGGGGTGTCGACTAACAGCCTTTATGCCTGGGTTAAGCGT
TACCAAAAACCTGAACCCCAACGCAAGCAGGATGATGCTATCCAGCTTGAAGTAAAACGTCTCAGGCAAGAACTTAAGCGGGTTACTGAAGAGCGAGATA
TTTTAAAAAAGGCCGCCGCGTACTTTGCAAGAACATCCGGCTGAAGTACGCCTTCATTAAAAAAATGTCGCCATTTTACCCTGTACGCCGCCTATGCCTG
GTTCTTGGCCTTCATTACAGCGGCTATTACCAGTGGCTAAAGCAGCCAGAATCGTTGCGCGCCCGACAGGACGAACGTCAGACTGGCCTCATTAAGCAAC
TGTGGCTTGAAAGTGGTACGGTTTATGGTTACCGGAAAATCTGGCTCGATATGAAAGACCTGGGTGAACGCGCCGGACGTAACAGGGTTGCGCGGTTAAT
GAGGCTCGCTGAATTAGCATCCCAGACCGGATATCGAAAACGACGTTACTACGGTGGTGGAAAACCTTCGGTAGCCAGCCCTAATTACCTTGAGCGACAG
TTCATTGTCCCAACTCCAAATACGCATTGGGTGAGTGACATGACCTATATAAGGACGCATGAAGGCTGGTTATATCTGGCTGTTGTTCTCGATCTCTTCT
CACGTCGGGTTATAGGCTGGAGCATGGGAGGACGAATGACTGCTGAGTTAGTGATGGATGCATTATTGATGGCGGTATGGCGACGTAAACCAACAGCAGA
AGTATTAATCCATTCAGATCAGGGTTCTCAATATACCAGCGAAAGGTGCCAGCATTTTATGGCATCTCACAATTTGAAAAGCAGCATGAGTCGTCGGGGT
AATTGCCATGACAACGCAGTGGCAGAAAGCTTTTTCCAGTTGCTAAAACGGGAACGCATAAAGAAACGGATCTATAAAAACAGAGAAGACGCGAAGGCGG
ATATCTTCGATTATGTAGAGATGTTTTATAATGCTAAACGCCGCCACAGTGCCTGTGAGGATCAACCCCCTGCAGTTTATGAAAGTGCCTGGTTCATGAG
GCACGGAACCGTCTAGGAAATCCGGGTCTATTCA
TGAAGCCGTAAAGCAGGTCACTGAGCAGGGATATCCCGTATCCGAGGTCGCCTCCCGACTTGGGGTGTCGACTAACAGCCTTTATGCCTGGGTTAAGCGT
TACCAAAAACCTGAACCCCAACGCAAGCAGGATGATGCTATCCAGCTTGAAGTAAAACGTCTCAGGCAAGAACTTAAGCGGGTTACTGAAGAGCGAGATA
TTTTAAAAAAGGCCGCCGCGTACTTTGCAAGAACATCCGGCTGAAGTACGCCTTCATTAAAAAAATGTCGCCATTTTACCCTGTACGCCGCCTATGCCTG
GTTCTTGGCCTTCATTACAGCGGCTATTACCAGTGGCTAAAGCAGCCAGAATCGTTGCGCGCCCGACAGGACGAACGTCAGACTGGCCTCATTAAGCAAC
TGTGGCTTGAAAGTGGTACGGTTTATGGTTACCGGAAAATCTGGCTCGATATGAAAGACCTGGGTGAACGCGCCGGACGTAACAGGGTTGCGCGGTTAAT
GAGGCTCGCTGAATTAGCATCCCAGACCGGATATCGAAAACGACGTTACTACGGTGGTGGAAAACCTTCGGTAGCCAGCCCTAATTACCTTGAGCGACAG
TTCATTGTCCCAACTCCAAATACGCATTGGGTGAGTGACATGACCTATATAAGGACGCATGAAGGCTGGTTATATCTGGCTGTTGTTCTCGATCTCTTCT
CACGTCGGGTTATAGGCTGGAGCATGGGAGGACGAATGACTGCTGAGTTAGTGATGGATGCATTATTGATGGCGGTATGGCGACGTAAACCAACAGCAGA
AGTATTAATCCATTCAGATCAGGGTTCTCAATATACCAGCGAAAGGTGCCAGCATTTTATGGCATCTCACAATTTGAAAAGCAGCATGAGTCGTCGGGGT
AATTGCCATGACAACGCAGTGGCAGAAAGCTTTTTCCAGTTGCTAAAACGGGAACGCATAAAGAAACGGATCTATAAAAACAGAGAAGACGCGAAGGCGG
ATATCTTCGATTATGTAGAGATGTTTTATAATGCTAAACGCCGCCACAGTGCCTGTGAGGATCAACCCCCTGCAGTTTATGAAAGTGCCTGGTTCATGAG
GCACGGAACCGTCTAGGAAATCCGGGTCTATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 66 | 344 | + | No |
Description : First part of the transposase
ORF sequence :
MSAKNFTEEFKIEAVKQVTEQGYPVSEVASRLGVSTNSLYAWVKRYQKPEPQRKQDDAIQLEVKRLRQELKRVTEERDILKKAAAYFARTSG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 290 | 1216 | + | No |
Description : Second part of the transposase
ORF sequence :
RARYFKKGRRVLCKNIRLKYAFIKKMSPFYPVRRLCLVLGLHYSGYYQWLKQPESLRARQDERQTGLIKQLWLESGTVYGYRKIWLDMKDLGERAGRNRV
ARLMRLAELASQTGYRKRRYYGGGKPSVASPNYLERQFIVPTPNTHWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTAELVMDALLMAVWRRK
PTAEVLIHSDQGSQYTSERCQHFMASHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNREDAKADIFDYVEMFYNAKRRHSACEDQPPAVYESA
WFMRHGTV
ARLMRLAELASQTGYRKRRYYGGGKPSVASPNYLERQFIVPTPNTHWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTAELVMDALLMAVWRRK
PTAEVLIHSDQGSQYTSERCQHFMASHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNREDAKADIFDYVEMFYNAKRRHSACEDQPPAVYESA
WFMRHGTV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 66 | 1216 | + | Yes |
Chemistry : DDE
ORF sequence :
MSAKNFTEEFKIEAVKQVTEQGYPVSEVASRLGVSTNSLYAWVKRYQKPEPQRKQDDAIQLEVKRLRQELKRVTEERDILKKGRRVLCKNIRLKYAFIKK
MSPFYPVRRLCLVLGLHYSGYYQWLKQPESLRARQDERQTGLIKQLWLESGTVYGYRKIWLDMKDLGERAGRNRVARLMRLAELASQTGYRKRRYYGGGK
PSVASPNYLERQFIVPTPNTHWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTAELVMDALLMAVWRRKPTAEVLIHSDQGSQYTSERCQHFMA
SHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNREDAKADIFDYVEMFYNAKRRHSACEDQPPAVYESAWFMRHGTV
MSPFYPVRRLCLVLGLHYSGYYQWLKQPESLRARQDERQTGLIKQLWLESGTVYGYRKIWLDMKDLGERAGRNRVARLMRLAELASQTGYRKRRYYGGGK
PSVASPNYLERQFIVPTPNTHWVSDMTYIRTHEGWLYLAVVLDLFSRRVIGWSMGGRMTAELVMDALLMAVWRRKPTAEVLIHSDQGSQYTSERCQHFMA
SHNLKSSMSRRGNCHDNAVAESFFQLLKRERIKKRIYKNREDAKADIFDYVEMFYNAKRRHSACEDQPPAVYESAWFMRHGTV
Blast result :
Comments
ISSpr1 is 82% (ORFA) and 79% (ORFB) aa similar to ISPsy29. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T.,Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission Genbank.