ISPar1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007204 | ND | Psychrobacter arcticus | Psychrobacter arcticus 273-4 |
DNA section
IS Length : 1277 bp
Ends
IR Length : 31/43
IRL : TGAGCTATGCTGAAGAAACCGTACAAGTAAACTTGGTAAACTAAGCACAA
IRR : TGACCCCTGCTGTCAAATCCGTACAGTTAAACTTGGGAGATTTTAATTAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTAGAT | AGT | TTGTCAT | 3 |
DNA sequence
TGAGCTATGCTGAAGAAACCGTACAAGTAAACTTGGTAAACTAAGCACAACAACAGGAGTTTACCATGACCAAGAAAATCAGAACCTATAGTGCAGAATT
CAAAGCAGAAGCCGTCAAGAAGATAGCGGACAACGATGGGAATATTTCAGCGACTGCAAAGCAGCTTGGTATCGCCATGCAGACCTTATCTAATTGGCAC
AATAAAGCCAATCAAGGCAAACTTGAGGGAACAGCTCAATATGATCCTGAACTCATAGCAGTGATCGAAGAAAACAAACGCCTCAAACGACAGCTTAAAG
TTGCCGAGGAGGAACGAGAGATCCTAAAAAAGGCGACGGCGTACTTTGCAAGGCACAGTTAGTGAGGTACGCCTTTATCAAAGATAACCAGCACTTCTTT
AGCATCACCACTATGTGCCGCGTGCTAAGTGTTAAGCCATCAAGCTATTACGACTGGCTAAGTCGTGATCTCAGCGACCAACAGATACATCGCAATCAGT
GTGAACTACTGGTTAAAGTCGCTCATACTGAGACCAAGCAGTGCTATGGCGTAGATCGACTGCATGCCCACCTAAGTGCGCAAGGGCACGATATCAGCCA
ATATATGGTTAGAAGCATCAAAGAAGAACATGGCATCAAATGCCGTCGCCACAAACGCTTTAAAGTCACCACCGACTCCAATCACAACAAGCTGGTCTAT
CCAAACGTACTGGATCAGAAGTTTGATGCCAGTCGCCCTAACCAAGCGTGGGTCAGTGACATCACCTATATTTGGACGCTTGAGGGCTGGTTGTATTTAG
CGGGGGTTAAGGACTTATACACCAAAGAGCTTGTTGGATATGCCATCAATAAGCGCATGACTGCTGATTTGGTTTGCCGTGCACTTAATATGGCAATTAA
GAATAAAGGGCCAAGCCAAGGGCTGACTGTGCATTCCGATAGAGGCAGCCAGTATTGTAGCCATGCGTACCATAATATTATCAAGCAGCATCATTTTAAA
GGCTCAATGAGTGGCAAAGGTAATTGTTATGACAACGCACCCATAGAAAGCTTCTGGGGCACATTAAAGAATGAGCTGGTATATCATCAAGACTATAAAA
CAAGGTTTACAGCCATTACCGATATCATTAGATATATCGAGCTGTATTACAATCAGACTAGGATTCAAAAGGGCTTGGGCTATAAATCGCCAAGACAGGT
GTGGTTTGACTTTTATCGTCATGCTGCGTAATTAAAATCTCCCAAGTTTAACTGTACGGATTTGACAGCAGGGGTCA
CAAAGCAGAAGCCGTCAAGAAGATAGCGGACAACGATGGGAATATTTCAGCGACTGCAAAGCAGCTTGGTATCGCCATGCAGACCTTATCTAATTGGCAC
AATAAAGCCAATCAAGGCAAACTTGAGGGAACAGCTCAATATGATCCTGAACTCATAGCAGTGATCGAAGAAAACAAACGCCTCAAACGACAGCTTAAAG
TTGCCGAGGAGGAACGAGAGATCCTAAAAAAGGCGACGGCGTACTTTGCAAGGCACAGTTAGTGAGGTACGCCTTTATCAAAGATAACCAGCACTTCTTT
AGCATCACCACTATGTGCCGCGTGCTAAGTGTTAAGCCATCAAGCTATTACGACTGGCTAAGTCGTGATCTCAGCGACCAACAGATACATCGCAATCAGT
GTGAACTACTGGTTAAAGTCGCTCATACTGAGACCAAGCAGTGCTATGGCGTAGATCGACTGCATGCCCACCTAAGTGCGCAAGGGCACGATATCAGCCA
ATATATGGTTAGAAGCATCAAAGAAGAACATGGCATCAAATGCCGTCGCCACAAACGCTTTAAAGTCACCACCGACTCCAATCACAACAAGCTGGTCTAT
CCAAACGTACTGGATCAGAAGTTTGATGCCAGTCGCCCTAACCAAGCGTGGGTCAGTGACATCACCTATATTTGGACGCTTGAGGGCTGGTTGTATTTAG
CGGGGGTTAAGGACTTATACACCAAAGAGCTTGTTGGATATGCCATCAATAAGCGCATGACTGCTGATTTGGTTTGCCGTGCACTTAATATGGCAATTAA
GAATAAAGGGCCAAGCCAAGGGCTGACTGTGCATTCCGATAGAGGCAGCCAGTATTGTAGCCATGCGTACCATAATATTATCAAGCAGCATCATTTTAAA
GGCTCAATGAGTGGCAAAGGTAATTGTTATGACAACGCACCCATAGAAAGCTTCTGGGGCACATTAAAGAATGAGCTGGTATATCATCAAGACTATAAAA
CAAGGTTTACAGCCATTACCGATATCATTAGATATATCGAGCTGTATTACAATCAGACTAGGATTCAAAAGGGCTTGGGCTATAAATCGCCAAGACAGGT
GTGGTTTGACTTTTATCGTCATGCTGCGTAATTAAAATCTCCCAAGTTTAACTGTACGGATTTGACAGCAGGGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 66 | 362 | + | No |
Description : First part of the transposase
ORF sequence :
MTKKIRTYSAEFKAEAVKKIADNDGNISATAKQLGIAMQTLSNWHNKANQGKLEGTAQYDPELIAVIEENKRLKRQLKVAEEEREILKKATAYFARHS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 933 bp | 310 aa | 299 | 1231 | + | No |
Description : Second part of the transposase
ORF sequence :
SCRGGTRDPKKGDGVLCKAQLVRYAFIKDNQHFFSITTMCRVLSVKPSSYYDWLSRDLSDQQIHRNQCELLVKVAHTETKQCYGVDRLHAHLSAQGHDIS
QYMVRSIKEEHGIKCRRHKRFKVTTDSNHNKLVYPNVLDQKFDASRPNQAWVSDITYIWTLEGWLYLAGVKDLYTKELVGYAINKRMTADLVCRALNMAI
KNKGPSQGLTVHSDRGSQYCSHAYHNIIKQHHFKGSMSGKGNCYDNAPIESFWGTLKNELVYHQDYKTRFTAITDIIRYIELYYNQTRIQKGLGYKSPRQ
VWFDFYRHAA
QYMVRSIKEEHGIKCRRHKRFKVTTDSNHNKLVYPNVLDQKFDASRPNQAWVSDITYIWTLEGWLYLAGVKDLYTKELVGYAINKRMTADLVCRALNMAI
KNKGPSQGLTVHSDRGSQYCSHAYHNIIKQHHFKGSMSGKGNCYDNAPIESFWGTLKNELVYHQDYKTRFTAITDIIRYIELYYNQTRIQKGLGYKSPRQ
VWFDFYRHAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 66 | 1231 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKKIRTYSAEFKAEAVKKIADNDGNISATAKQLGIAMQTLSNWHNKANQGKLEGTAQYDPELIAVIEENKRLKRQLKVAEEEREILKKGDGVLCKAQLV
RYAFIKDNQHFFSITTMCRVLSVKPSSYYDWLSRDLSDQQIHRNQCELLVKVAHTETKQCYGVDRLHAHLSAQGHDISQYMVRSIKEEHGIKCRRHKRFK
VTTDSNHNKLVYPNVLDQKFDASRPNQAWVSDITYIWTLEGWLYLAGVKDLYTKELVGYAINKRMTADLVCRALNMAIKNKGPSQGLTVHSDRGSQYCSH
AYHNIIKQHHFKGSMSGKGNCYDNAPIESFWGTLKNELVYHQDYKTRFTAITDIIRYIELYYNQTRIQKGLGYKSPRQVWFDFYRHAA
RYAFIKDNQHFFSITTMCRVLSVKPSSYYDWLSRDLSDQQIHRNQCELLVKVAHTETKQCYGVDRLHAHLSAQGHDISQYMVRSIKEEHGIKCRRHKRFK
VTTDSNHNKLVYPNVLDQKFDASRPNQAWVSDITYIWTLEGWLYLAGVKDLYTKELVGYAINKRMTADLVCRALNMAIKNKGPSQGLTVHSDRGSQYCSH
AYHNIIKQHHFKGSMSGKGNCYDNAPIESFWGTLKNELVYHQDYKTRFTAITDIIRYIELYYNQTRIQKGLGYKSPRQVWFDFYRHAA
Blast result :
Comments
ISPar1 is 79% (ORFA) and 81% (ORFB) aa similar to ISPssp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Ayala-del-Rio,H.L., Chain,P., Ponder,M.A., Di Bartolo,G., Ivanova,N., Bergholz,P.W., Hauser,L., Land,M., Bakermans,C., Rodrigues,D., Klappenbach,J.A., Zarka,D., Larimer,F., Richardson,P., Thomashow,M.F. and Tiedje,J.M. (2006) Direct submission GenBank.