ISBce13
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AALL01000034 | ND | Bacillus cereus | Bacillus cereus subsp cytotoxis NVH 391-98 |
DNA section
IS Length : 1253 bp
Ends
IR Length : 30/52
IRL : TGGAGTTAGTATAGTCATATGGACACAACTTAGTTAAGTCCTTACAATGG
IRR : TGGATTTGAGTCAATATTTTGGACACAAAAAAGTTAAATCTAAGCTACTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCTTGACTG | ATTTTTATAA | 0 |
DNA sequence
TGGAGTTAGTATAGTCATATGGACACAACTTAGTTAAGTCCTTACAATGGATTTCAAGGAGGAAGTGTCCGTATGGCAAACAAGAACTTTAATGAAGAAT
TTAAAAAGATGGTTGTTGAATTATATCATTCAGACCAATCTGTGAAAGAATTAAGCAGCGAGTATGGTGTATCAGAAGTAACGATTTATAAATGGATTAA
AAAATATTCTCCTATCCCAACGATTGATGAGGAAGAAATGACTCCAGAAGATCTAAAACGAATGCAAAAAGAAATGCTTCGCCTGAAAGAGGAGAATGAG
ATATTAAAAAAGGCTATGGCCATATTCGCGAAAAAATAAACGATACAGAATTACATCAATTTATCGATTGTCAAAAAGAAACACACTCTGTTCGTACAAT
GTGTCAAACACTTGGAATAGCGAGAAGTTCGTATTACCAATCTCAGCACAAAACAGAGTCAAAGCGTAGTCGTGAAAATGAAGAGTTAACCAAACAAATT
ATACAAATTCATCAAGATAGTGGAGGGCGTTACGGTGCACCGAAAATTCATCAAACCCTATTGGAACAAGGGTTTCAAGTAAGTGTAAAGCGTGTGCAAC
GACTCATGAAAAAAGAAAATATTCGCTCCATCATCTTAAAGAAATATAAACCACATTCATCAAAATCAACTATAGAAGAACGAATAAATCTACTAGAACA
GGACTTCTCTACAACGACAATTAATGAAAAGTGGGTGGCGGATATTACTTATATTCACACGCAAAAAGATGGCTGGTGTTACCTTGCATCAGTGATGGAT
TTGCATTCTAAGAAAATTATTGGATATTCATTTTCACGAAATATGACAACGAACCTTGTGGTAAAAGCTTTAGAAAACGCTTACCATACACAAAGACCAC
AAAAAGGGCTCATTTTACATACTGATTTAGGAACACAATATACAAGTCAGGAATTTCAAACTTTACTCGCGAATTATAAAATAAAGCCTTCTTTCAGTAA
AAAAGGTTGTCCATACGATAACGCTTGTATTGAGTCATTTCACGCCATTTTAAAGAAAGAAGAAGTATATCGTACAAAATATGTCTCATTTGAAGAAGCA
AATCTAGCACTATTTCAATACATTGAGGGATTTTATAATCGTAAGCGAATCCATAGTAGCATCGGTTATAAAACTCCTCAAACAATAGAAGATCTGGCTA
TAAAAGTAGCTTAGATTTAACTTTTTTGTGTCCAAAATATTGACTCAAATCCA
TTAAAAAGATGGTTGTTGAATTATATCATTCAGACCAATCTGTGAAAGAATTAAGCAGCGAGTATGGTGTATCAGAAGTAACGATTTATAAATGGATTAA
AAAATATTCTCCTATCCCAACGATTGATGAGGAAGAAATGACTCCAGAAGATCTAAAACGAATGCAAAAAGAAATGCTTCGCCTGAAAGAGGAGAATGAG
ATATTAAAAAAGGCTATGGCCATATTCGCGAAAAAATAAACGATACAGAATTACATCAATTTATCGATTGTCAAAAAGAAACACACTCTGTTCGTACAAT
GTGTCAAACACTTGGAATAGCGAGAAGTTCGTATTACCAATCTCAGCACAAAACAGAGTCAAAGCGTAGTCGTGAAAATGAAGAGTTAACCAAACAAATT
ATACAAATTCATCAAGATAGTGGAGGGCGTTACGGTGCACCGAAAATTCATCAAACCCTATTGGAACAAGGGTTTCAAGTAAGTGTAAAGCGTGTGCAAC
GACTCATGAAAAAAGAAAATATTCGCTCCATCATCTTAAAGAAATATAAACCACATTCATCAAAATCAACTATAGAAGAACGAATAAATCTACTAGAACA
GGACTTCTCTACAACGACAATTAATGAAAAGTGGGTGGCGGATATTACTTATATTCACACGCAAAAAGATGGCTGGTGTTACCTTGCATCAGTGATGGAT
TTGCATTCTAAGAAAATTATTGGATATTCATTTTCACGAAATATGACAACGAACCTTGTGGTAAAAGCTTTAGAAAACGCTTACCATACACAAAGACCAC
AAAAAGGGCTCATTTTACATACTGATTTAGGAACACAATATACAAGTCAGGAATTTCAAACTTTACTCGCGAATTATAAAATAAAGCCTTCTTTCAGTAA
AAAAGGTTGTCCATACGATAACGCTTGTATTGAGTCATTTCACGCCATTTTAAAGAAAGAAGAAGTATATCGTACAAAATATGTCTCATTTGAAGAAGCA
AATCTAGCACTATTTCAATACATTGAGGGATTTTATAATCGTAAGCGAATCCATAGTAGCATCGGTTATAAAACTCCTCAAACAATAGAAGATCTGGCTA
TAAAAGTAGCTTAGATTTAACTTTTTTGTGTCCAAAATATTGACTCAAATCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 73 | 339 | + | No |
Description : First part of the transposase
ORF sequence :
MANKNFNEEFKKMVVELYHSDQSVKELSSEYGVSEVTIYKWIKKYSPIPTIDEEEMTPEDLKRMQKEMLRLKEENEILKKAMAIFAKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 915 bp | 304 aa | 300 | 1214 | + | No |
Description : Second part of the transposase
ORF sequence :
GDIKKGYGHIREKINDTELHQFIDCQKETHSVRTMCQTLGIARSSYYQSQHKTESKRSRENEELTKQIIQIHQDSGGRYGAPKIHQTLLEQGFQVSVKRV
QRLMKKENIRSIILKKYKPHSSKSTIEERINLLEQDFSTTTINEKWVADITYIHTQKDGWCYLASVMDLHSKKIIGYSFSRNMTTNLVVKALENAYHTQR
PQKGLILHTDLGTQYTSQEFQTLLANYKIKPSFSKKGCPYDNACIESFHAILKKEEVYRTKYVSFEEANLALFQYIEGFYNRKRIHSSIGYKTPQTIEDL
AIKVA
QRLMKKENIRSIILKKYKPHSSKSTIEERINLLEQDFSTTTINEKWVADITYIHTQKDGWCYLASVMDLHSKKIIGYSFSRNMTTNLVVKALENAYHTQR
PQKGLILHTDLGTQYTSQEFQTLLANYKIKPSFSKKGCPYDNACIESFHAILKKEEVYRTKYVSFEEANLALFQYIEGFYNRKRIHSSIGYKTPQTIEDL
AIKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1142 bp | 380 aa | 73 | 1214 | + | Yes |
Chemistry : DDE
ORF sequence :
MANKNFNEEFKKMVVELYHSDQSVKELSSEYGVSEVTIYKWIKKYSPIPTIDEEEMTPEDLKRMQKEMLRLKEENEILKKGYGHIREKINDTELHQFIDC
QKETHSVRTMCQTLGIARSSYYQSQHKTESKRSRENEELTKQIIQIHQDSGGRYGAPKIHQTLLEQGFQVSVKRVQRLMKKENIRSIILKKYKPHSSKST
IEERINLLEQDFSTTTINEKWVADITYIHTQKDGWCYLASVMDLHSKKIIGYSFSRNMTTNLVVKALENAYHTQRPQKGLILHTDLGTQYTSQEFQTLLA
NYKIKPSFSKKGCPYDNACIESFHAILKKEEVYRTKYVSFEEANLALFQYIEGFYNRKRIHSSIGYKTPQTIEDLAIKVA
QKETHSVRTMCQTLGIARSSYYQSQHKTESKRSRENEELTKQIIQIHQDSGGRYGAPKIHQTLLEQGFQVSVKRVQRLMKKENIRSIILKKYKPHSSKST
IEERINLLEQDFSTTTINEKWVADITYIHTQKDGWCYLASVMDLHSKKIIGYSFSRNMTTNLVVKALENAYHTQRPQKGLILHTDLGTQYTSQEFQTLLA
NYKIKPSFSKKGCPYDNACIESFHAILKKEEVYRTKYVSFEEANLALFQYIEGFYNRKRIHSSIGYKTPQTIEDLAIKVA
Blast result :
Comments
ISBce13 is 67 % (ORFA) aa similar to IS981 and 72% (ORFB) aa similar to ISCb3.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Lapidus,A., Goltsman,E., Copeland,A., Lucas,S., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S., Richardson,P., Auger,S., Galleron,N., Sanchis,V., Gohar,M., Broussole,V., Brillard,J., Guinebretiere,M.-H., Ehrlich,S.D., Lereclus,D., Aymerich,S., Nguen-the,C. and Sorokin,A. (2006) Direct Submission GenBank.