ISBad2
- Family IS3
- Group IS150
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_008618 | ND | Bifidobacterium adolescentis | Bifidobacterium adolescentis ATCC 1573 |
DNA section
IS Length : 1470 bp
Ends
IR Length : 21/25
IRL : TGAACCGCACCCCGATTGTTGGACTGAAGAAATTCAGATTCGATGATCGG
IRR : TGAACTGCGCCCCGTTTGTTGGACGTTGTTAATTGGGATACAGGGTCAGA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CCCGGCGTAA | ATA | ACCGCCGGGGCCTT | 3 |
DNA sequence
TGAACCGCACCCCGATTGTTGGACTGAAGAAATTCAGATTCGATGATCGGAGGTGCGGTTCTTTCGTATGCGTGAGGATCGAAGGAGACGTTACGACGAC
GGGTTCCGGCGCGAGGCGCTGGAGCTCATCAAGTCCGGAGCCGGCAAGGACACGCTCGCCAGAAGGCTTGCCATACCGGTGTATATCGCGAGAAACTGGA
TCGGGCTGTACAGGTCCAACGGCGAGGAGGCGGTCATGGGAGGCGGCGGCAGCAGACGCTACGACTGGGAGACGAAGGTCGCCGCGTCGCGCGACCACGT
CGAGAACGGTCTGACCAAGACGGAGGCCATGGCCAGGCACGGGATCGCGAGCATCGCGTCCCTGGAGCGCTGGTGCCGCGACTACCGGTCCGGAGGCGCT
CAGGCCGAAACCCAAGGGCAGGCCGAAAGGCGCGAAATCCAAACCGAAGCCCGAACCGACGCGCGAGCAGGAGCTCGCCGAACAGGTCGCGTACCTGAAG
GCGAAGGTCGCGTACCTGGAAAAACTCCGGGCCCTGCGGGCGTCCAAGTCACGCGGCGCGAGCGAAGCGCCGTCGTCCGACTGCTCGCAGGGCGGGGACA
CCGGCTCGACCACCTCCTGAGGATAGCCGGGCTGCCGAAATCCACGTACTACCACCACCTGTCGCGCCCGGCACACGTGTCCAGGCCGGACGTGGAGCCG
ATGGTACGGGAGATATGGGAGCGCACCCCGAACGGGTGCGGCCACCGGCAGGTGCGCATGTGCCTGGTCCACGAGTTCGGCACGCGCATATCCGCCAAGA
CCGTGCTCAGGGTCATGCGCCGTATGGGCTGAGATGCGCCATCCGCTCCAGGAACCCGTGGATACGCTACAGCTCGTACAGGGGCGAGACGGGCGACCGC
GTGCATAACCTGCCCGAACGCGACTTCGACGCGGCCCGTCCGTTCTCCAGGCTCGGCACCGACGTCACCGAGTTCAAGGTCGCCGGATCCAAGGCGTACC
TCGCTCCCGTGTACGACATGGCCAGCAAGGAGATCGTGGCCTGGGACGTGAGCCGCAGTCCGGACCTCGGTCAGCAGAAGCGTCTGCCCGCCATGCTCGC
GGAGCGCCTGCCCGCCGGCGCGGAGCCGATCCTGCACTCGGACATGGGATGGCAGTACCAGCACCAATGGTGGAGGAAGGAGCTCGAACGCCTCGGCATC
CGCCGGTCCATGAGCCGCAAGGGCAACTGCCTGGACAACGCGGCGGCCGAGCAGGTCTTCGGCCACTTGAAGGACGAGTTCTACCGCGGCCGTGTGTTCG
ACTCGTACGAACAGTTCAAACGCGAGCTGGACGCCTACATCATCCATTGGAACACCAGACGACGCCAGATACGACTCGAGGGACGCACCCCGGAGGAGTT
CCGGAGCATGTCCCTCGCGGTCTGACCCTGTATCCCAATTAACAACGTCCAACAAACGGGGCGCAGTTCA
GGGTTCCGGCGCGAGGCGCTGGAGCTCATCAAGTCCGGAGCCGGCAAGGACACGCTCGCCAGAAGGCTTGCCATACCGGTGTATATCGCGAGAAACTGGA
TCGGGCTGTACAGGTCCAACGGCGAGGAGGCGGTCATGGGAGGCGGCGGCAGCAGACGCTACGACTGGGAGACGAAGGTCGCCGCGTCGCGCGACCACGT
CGAGAACGGTCTGACCAAGACGGAGGCCATGGCCAGGCACGGGATCGCGAGCATCGCGTCCCTGGAGCGCTGGTGCCGCGACTACCGGTCCGGAGGCGCT
CAGGCCGAAACCCAAGGGCAGGCCGAAAGGCGCGAAATCCAAACCGAAGCCCGAACCGACGCGCGAGCAGGAGCTCGCCGAACAGGTCGCGTACCTGAAG
GCGAAGGTCGCGTACCTGGAAAAACTCCGGGCCCTGCGGGCGTCCAAGTCACGCGGCGCGAGCGAAGCGCCGTCGTCCGACTGCTCGCAGGGCGGGGACA
CCGGCTCGACCACCTCCTGAGGATAGCCGGGCTGCCGAAATCCACGTACTACCACCACCTGTCGCGCCCGGCACACGTGTCCAGGCCGGACGTGGAGCCG
ATGGTACGGGAGATATGGGAGCGCACCCCGAACGGGTGCGGCCACCGGCAGGTGCGCATGTGCCTGGTCCACGAGTTCGGCACGCGCATATCCGCCAAGA
CCGTGCTCAGGGTCATGCGCCGTATGGGCTGAGATGCGCCATCCGCTCCAGGAACCCGTGGATACGCTACAGCTCGTACAGGGGCGAGACGGGCGACCGC
GTGCATAACCTGCCCGAACGCGACTTCGACGCGGCCCGTCCGTTCTCCAGGCTCGGCACCGACGTCACCGAGTTCAAGGTCGCCGGATCCAAGGCGTACC
TCGCTCCCGTGTACGACATGGCCAGCAAGGAGATCGTGGCCTGGGACGTGAGCCGCAGTCCGGACCTCGGTCAGCAGAAGCGTCTGCCCGCCATGCTCGC
GGAGCGCCTGCCCGCCGGCGCGGAGCCGATCCTGCACTCGGACATGGGATGGCAGTACCAGCACCAATGGTGGAGGAAGGAGCTCGAACGCCTCGGCATC
CGCCGGTCCATGAGCCGCAAGGGCAACTGCCTGGACAACGCGGCGGCCGAGCAGGTCTTCGGCCACTTGAAGGACGAGTTCTACCGCGGCCGTGTGTTCG
ACTCGTACGAACAGTTCAAACGCGAGCTGGACGCCTACATCATCCATTGGAACACCAGACGACGCCAGATACGACTCGAGGGACGCACCCCGGAGGAGTT
CCGGAGCATGTCCCTCGCGGTCTGACCCTGTATCCCAATTAACAACGTCCAACAAACGGGGCGCAGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence : No
- Secondary structure : stem-loop
Recoding motif :
AAAAACTCCGGGCCCTGCGGGCGTCCAAGTCACGCGGCGCGAGCGAAGCG
-----------(((((((((((-----(((((((((((((-------)))
CCGTCGTCCGACTGCTCGCAGGGC
)))-)))--)))))))))))))))
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
768 bp | 254 aa | 65 | 832 | + | No |
Description : First part of the transposase
ORF sequence :
MREDRRRRYDDGFRREALELIKSGAGKDTLARRLAIPVYIARNWIGLYRSNGEEAVMGGGGSRRYDWETKVAASRDHVENGLTKTEAMARHGIASIASLE
RWCRDYRSGGAQAETQGQAERREIQTEARTDARAGARRTGRVPEGEGRVPGKTPGPAGVQVTRRERSAVVRLLAGRGHRLDHLLRIAGLPKSTYYHHLSR
PAHVSRPDVEPMVREIWERTPNGCGHRQVRMCLVHEFGTRISAKTVLRVMRRMG
RWCRDYRSGGAQAETQGQAERREIQTEARTDARAGARRTGRVPEGEGRVPGKTPGPAGVQVTRRERSAVVRLLAGRGHRLDHLLRIAGLPKSTYYHHLSR
PAHVSRPDVEPMVREIWERTPNGCGHRQVRMCLVHEFGTRISAKTVLRVMRRMG
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
927 bp | 308 aa | 499 | 1425 | + | No |
Description : Second part of the transposase
ORF sequence :
RRRSRTWKNSGPCGRPSHAARAKRRRPTARRAGTPARPPPEDSRAAEIHVLPPPVAPGTRVQAGRGADGTGDMGAHPERVRPPAGAHVPGPRVRHAHIRQ
DRAQGHAPYGLRCAIRSRNPWIRYSSYRGETGDRVHNLPERDFDAARPFSRLGTDVTEFKVAGSKAYLAPVYDMASKEIVAWDVSRSPDLGQQKRLPAML
AERLPAGAEPILHSDMGWQYQHQWWRKELERLGIRRSMSRKGNCLDNAAAEQVFGHLKDEFYRGRVFDSYEQFKRELDAYIIHWNTRRRQIRLEGRTPEE
FRSMSLAV
DRAQGHAPYGLRCAIRSRNPWIRYSSYRGETGDRVHNLPERDFDAARPFSRLGTDVTEFKVAGSKAYLAPVYDMASKEIVAWDVSRSPDLGQQKRLPAML
AERLPAGAEPILHSDMGWQYQHQWWRKELERLGIRRSMSRKGNCLDNAAAEQVFGHLKDEFYRGRVFDSYEQFKRELDAYIIHWNTRRRQIRLEGRTPEE
FRSMSLAV
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1361 bp | 453 aa | 65 | 1425 | + | Yes |
Chemistry : DDE
ORF sequence :
MREDRRRRYDDGFRREALELIKSGAGKDTLARRLAIPVYIARNWIGLYRSNGEEAVMGGGGSRRYDWETKVAASRDHVENGLTKTEAMARHGIASIASLE
RWCRDYRSGGAQAETQGQAERREIQTEARTDARAGARRTGRVPEGEGRVPGKKNSGPCGRPSHAARAKRRRPTARRAGTPARPPPEDSRAAEIHVLPPPV
APGTRVQAGRGADGTGDMGAHPERVRPPAGAHVPGPRVRHAHIRQDRAQGHAPYGLRCAIRSRNPWIRYSSYRGETGDRVHNLPERDFDAARPFSRLGTD
VTEFKVAGSKAYLAPVYDMASKEIVAWDVSRSPDLGQQKRLPAMLAERLPAGAEPILHSDMGWQYQHQWWRKELERLGIRRSMSRKGNCLDNAAAEQVFG
HLKDEFYRGRVFDSYEQFKRELDAYIIHWNTRRRQIRLEGRTPEEFRSMSLAV
RWCRDYRSGGAQAETQGQAERREIQTEARTDARAGARRTGRVPEGEGRVPGKKNSGPCGRPSHAARAKRRRPTARRAGTPARPPPEDSRAAEIHVLPPPV
APGTRVQAGRGADGTGDMGAHPERVRPPAGAHVPGPRVRHAHIRQDRAQGHAPYGLRCAIRSRNPWIRYSSYRGETGDRVHNLPERDFDAARPFSRLGTD
VTEFKVAGSKAYLAPVYDMASKEIVAWDVSRSPDLGQQKRLPAMLAERLPAGAEPILHSDMGWQYQHQWWRKELERLGIRRSMSRKGNCLDNAAAEQVFG
HLKDEFYRGRVFDSYEQFKRELDAYIIHWNTRRRQIRLEGRTPEEFRSMSLAV
Blast result :
Comments
ISBad2 is 61% aa similar to ISBlo3.
The third ORF is the putative ORFAB transposase reconstructed in siclio by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in siclio by possible -1 frameshift.
References
1] Suzuki,T., Tsuda,Y., Kanou,N., Inoue,T., Kumazaki,K., Nagano,S., Hirai,S., Tanaka,K. and Watanabe,K.(2006) Direct submission GenBank.