ISRhba2

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Rhodobacterales bacterium
Rhodobacterales bacterium HTCC2150
DNA section
IS Length : 1231 bp

Ends


IR Length : 25/29

IRL : TGGACCTGTCACGGTTTGATGCCGCTCCTTTGATAGCATTTACTGCAGCA
IRR : TGGACCTGTCGCGCTTTTGTGCCGCCCCCGGTGCTCGTTTAAGCCACCTT

Insertion site


Left flankDirect repeatRight flankDR Length
GCCCTGATAGATGCGGCGAC0

DNA sequence

TGGACCTGTCACGGTTTGATGCCGCTCCTTTGATAGCATTTACTGCAGCAAAGGAGACTGACTATGGTACTGAAACGGACGGACGAATTCCGCCAAGATG
CAGTGCGGATCGCGCTGACCAGTGGGCTGACGCGCAAGCAGGTGGCGGACGATCTTGGCGTCGGCATGTCGACACTGAACAAATGGATCACGGCGCATCG
AGACACGGATTTGGTGTCAAAAGAGGATTTAAGCCTCGCCCAAGAGAATGACCGGCTTCGACGTGAGAACCGCATTCTCAAGGAGGAAAGGGAAGTGTTA
AAAAAGGCCACGGTGTTCTTTGCGAGCCAAAAGCCATGAGGTTTAAGTTTATCGAAGAACACCGATCTGCATTTTCCATTGAACTGATGTGTCGGGTCAT
GGCTGTCAGTCCGCGCGGTTTGCGGGCGTATCGCAGCCGACCTGCCAGCCGCAGACAGCGGTCTGATATGGTGACGTTGGCGCACATTAAAGAACAATCG
CGCCTGAGCTTGGGCAGCTATGGCCGCCCACGCATGACAGAAGAGCTGAAAGAGATTGGCCTTGATATTGGTCACCGCCGTGTTGGGCGTTTGATGCGCC
AGAATGGCATATCAGTGATCAGAACACGCAAACATAAGGTGACGACAGACAGCAATCATAAGTTCAACATCTCGCCGAACCTACTGGAGCGTGACTTCAA
CGCAGATGCGCCAAATCAGAAGTGGGTTGGTGACATTACCTATATCTGGACGCGTGAGGGCTGGTTGTATCTGGCCGTGATCCTCGATCTTCATTCACGG
CGTGTGATCGGCTGGGCGGTGAGCAATCGGATGAAACGAGACCTGGCAATCAGGGCGTTGAAAATGGCTATCGCGTTCCGCGCACCTGCAAAAGGCTGCA
TCCATCACACTGACCGTGGCAGCCAATATTGCTCACACGATTACCAAAAGATCCTGCGCCAGCATGGCTTCAAAGTATCCATGTCTGGCAAAGGCAATTG
CTATGATAATGCCGCCGTCGAAACCTTCTTCAAAACCATCAAGGCGGAATTGATCTGGCGCGATAACTGGGAAACCCGCAGGAAGGCTGAGATGGCGATC
TTCGAATACATCAACGGCTTCTACAATCCACGACGACGCCATTCAGCATTAGGCTGGAAAAGCCCTGTCGCATTCGAACGAAAGGTGGCTTAAACGAGCA
CCGGGGGCGGCACAAAAGCGCGACAGGTCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
276 bp91 aa64339+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MVLKRTDEFRQDAVRIALTSGLTRKQVADDLGVGMSTLNKWITAHRDTDLVSKEDLSLAQENDRLRRENRILKEEREVLKKATVFFASQKP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
927 bp308 aa2671193+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

EPHSQGGKGSVKKGHGVLCEPKAMRFKFIEEHRSAFSIELMCRVMAVSPRGLRAYRSRPASRRQRSDMVTLAHIKEQSRLSLGSYGRPRMTEELKEIGLD
IGHRRVGRLMRQNGISVIRTRKHKVTTDSNHKFNISPNLLERDFNADAPNQKWVGDITYIWTREGWLYLAVILDLHSRRVIGWAVSNRMKRDLAIRALKM
AIAFRAPAKGCIHHTDRGSQYCSHDYQKILRQHGFKVSMSGKGNCYDNAAVETFFKTIKAELIWRDNWETRRKAEMAIFEYINGFYNPRRRHSALGWKSP
VAFERKVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1130 bp376 aa641193+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVLKRTDEFRQDAVRIALTSGLTRKQVADDLGVGMSTLNKWITAHRDTDLVSKEDLSLAQENDRLRRENRILKEEREVLKKGHGVLCEPKAMRFKFIEEH
RSAFSIELMCRVMAVSPRGLRAYRSRPASRRQRSDMVTLAHIKEQSRLSLGSYGRPRMTEELKEIGLDIGHRRVGRLMRQNGISVIRTRKHKVTTDSNHK
FNISPNLLERDFNADAPNQKWVGDITYIWTREGWLYLAVILDLHSRRVIGWAVSNRMKRDLAIRALKMAIAFRAPAKGCIHHTDRGSQYCSHDYQKILRQ
HGFKVSMSGKGNCYDNAAVETFFKTIKAELIWRDNWETRRKAEMAIFEYINGFYNPRRRHSALGWKSPVAFERKVA

 

Blast result :
Comments
ISRhba2 is 95% (ORFA) and 93% (ORFB) aa similar to ISRosp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Giovannoni,S., Vergin,K., Ferriera,S., Johnson,J., Kravitz,S.,Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. andVenter,J.C. (2006) Direct submission GenBank.