ISNmu1

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007614 ND Nitrosospira multiformis
Nitrosospira multiformis ATCC 25196
DNA section
IS Length : 1221 bp

Ends


IR Length : 21/25

IRL : GGGTTTTCCCCCGATTTCACGGACACATCTAAAAAGGCTGATAATGAGTC
IRR : GGGTTTTACCCCATTTCTCCGGACGGTTTGGTTAAGCCTAAATCCTGCTT

Insertion site


Left flankDirect repeatRight flankDR Length
AAGAAGAGCACAGCAAAAGGCG2

DNA sequence

GGGTTTTCCCCCGATTTCACGGACACATCTAAAAAGGCTGATAATGAGTCAGGAGGTGTGTCATGGCAACGCGAAGGAAATATGCAGCGGAGTTCAAGCA
GGAAGCGGTACAACTAGTTCGGTCTGGCAATGTGCCGGCAAGCCGGATCGCGCGTGATCTGGGGATTGATCCCAATATGCTGAGCCGGTGGTGCCGGGAA
ATGGGCTCGGTCGGTAATAAAGCCTTTCAGGGTCAGGGCAGGCCCCGTGATGAGGAGATGGCTGCGCTCAAACGTGAGCTGATCCAAGTTAGGAAGGAGC
GTGATTTTTTAAAAGAAGCGGCGGCGTTATTCGCGAAAGCGTCGAAATGAGATATCGCATGATCGAACGCTGCCGCGATGCCTTCCCCATTCGCCTGATG
TGTCGTTGTCTGCATGTATCAACCAGCGGCTACTATGATTGGCGAGATCGCCCCTTGAGTCAGAGAGCGCATGATGACCGGCGTTTGCTGGAGCGCATCA
GGAAGATTCATGATGGCAGCGATGGGGTGATGGGCAGTCCTCGAGTCTGGAAGGAGTTGCGTTATGAAGGCGAGGCGTGTGGGTTGAACCGCATAGCACG
GCTGATGCGCCAGCAGAGGTTGTATGGCATCCCGCAGCGTAAACGCTGGCACAAGAAGGTCTGTGGCCTTCGACCGGAGAATGTGCACAATCACTTGCAG
CGGGACTTTACAGCAAGTGAGCCCAACAGCCGGTGGGTGACGGACATAACCTATGTGCGCACTCAGGAAAGCTGGCTTTATCTGTGCGTGGTCGTGGACC
TGTACTCGGGCATCGTGGTCGGCTGGTCAATGAGCCACTGCCAGGATCCGCAACTGGTGCTGCAGGCCGTACTCATGGCGCTTTGGCAGCGTGAGGCAAA
AACTCCCGTCATCCTGCACTCAGATCGCGGCTGTCAGTTTACCAGTGACGAGTATCAACGCTTCCTGCAAGGACACAATCTGATTTGCAGCATGAGCGCT
GTCGGTAGTTGTGCCGACAACGCGCTAGCAGAGAGCTTCTTTGGCTTGCTCAAGCGGGAGCGAGTATATCGTCGCCAGTATCGAACCCGGGCTGAGGCGC
GCACAGATATCTTCGACTATATCGAGCGATTCCATAATCCGCGAAAACGGCGACAGCTGGAAATGCGTCAGAAGCAGGATTTAGGCTTAACCAAACCGTC
CGGAGAAATGGGGTAAAACCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
288 bp95 aa63350+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MATRRKYAAEFKQEAVQLVRSGNVPASRIARDLGIDPNMLSRWCREMGSVGNKAFQGQGRPRDEEMAALKRELIQVRKERDFLKEAAALFAKASK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
912 bp303 aa3051216+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

FFKRSGGVIRESVEMRYRMIERCRDAFPIRLMCRCLHVSTSGYYDWRDRPLSQRAHDDRRLLERIRKIHDGSDGVMGSPRVWKELRYEGEACGLNRIARL
MRQQRLYGIPQRKRWHKKVCGLRPENVHNHLQRDFTASEPNSRWVTDITYVRTQESWLYLCVVVDLYSGIVVGWSMSHCQDPQLVLQAVLMALWQREAKT
PVILHSDRGCQFTSDEYQRFLQGHNLICSMSAVGSCADNALAESFFGLLKRERVYRRQYRTRAEARTDIFDYIERFHNPRKRRQLEMRQKQDLGLTKPSG
EMG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1154 bp384 aa631216+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MATRRKYAAEFKQEAVQLVRSGNVPASRIARDLGIDPNMLSRWCREMGSVGNKAFQGQGRPRDEEMAALKRELIQVRKERDFFKRSGGVIRESVEMRYRM
IERCRDAFPIRLMCRCLHVSTSGYYDWRDRPLSQRAHDDRRLLERIRKIHDGSDGVMGSPRVWKELRYEGEACGLNRIARLMRQQRLYGIPQRKRWHKKV
CGLRPENVHNHLQRDFTASEPNSRWVTDITYVRTQESWLYLCVVVDLYSGIVVGWSMSHCQDPQLVLQAVLMALWQREAKTPVILHSDRGCQFTSDEYQR
FLQGHNLICSMSAVGSCADNALAESFFGLLKRERVYRRQYRTRAEARTDIFDYIERFHNPRKRRQLEMRQKQDLGLTKPSGEMG

 

Blast result :
Comments
ISNmu1 is 59 % (ORFA) aa similar to ISAma1 and 65% (ORFB) aa similar to ISAisp2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The terminal inverted repeat are not expected ends for an IS3 family members.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P. (2005) Direct Submission GenBank.