ISPen2
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008027 | ND | Pseudomonas entomophila | Pseudomonas entomophila L48 Pseudomonas sp. LM15 |
DNA section
IS Length : 1232 bp
Ends
IR Length : 35/39
IRL : TGAATCGCCCCTGGTTTCGTAGACACCTCCAAGCCTCATAATGAGGCCCA
IRR : TGAATCGCCCCTGGTTTTCTAGACACTCTCCAAGCTCAGAAAATGGCGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACCACTCTC | GCGGTAGGTG | 0 | |
| GACAACTGCA | CAA | GTTAACGTAT | 3 |
DNA sequence
TGAATCGCCCCTGGTTTCGTAGACACCTCCAAGCCTCATAATGAGGCCCAAATAGGAGGTGCCATGAGTCGTCAGCGTTACCCCGAAGAATTCAAGATCG
AAGCGGTCAAGCAAGTGACCGAAAAAGGCAAACCTGTCGCCGAGGTCGCCCAGCGCCTGGGCATGTCCGTACACAGCCTTTATGCCTGGATCAAGGTCTA
CAGCAAGCCCCAAGAGCAGCGTCAGCAAGACAATGATCAGCAGGCTGAACTGCGCAAGCTGCGCGCTGAACTCAAGCGGGTGACAGAAGAGCGAGACATC
TTAAAGAAGGCCGCCGCGTACTTTGCCAAGGAGTGCGGCTGAAGTACGCCTTCATCAACAAGCACTCGACGGATTACCCAGTGCGTCGCCTTTGCCAAAC
CCTCAAGGTGCATCCCAGCGGCTACTACGCTTGGTTGGCCGAGCCTCAATCTGTCCGAGCCAAAGAAGATCAGCGCCTGCTTGGCTTGATCAAGCAGGCC
TGGTTAGAAAGCGGCGGCGTGTATGGCTATCGCAAGATTCACGATGACCTGCGAGAGCTAGGAGAGATCTGTGGGCGAAATCGAGTCGGTCGCTTGATGC
AGGCAGAGGGGCTGCGTTCGCAAACGGGCCATCGCCGTCGTACTGGGTTTTATAGCGGAAAACCAACAGCGGCATCGCCCAACCATCTGGCCCGGCAGTT
CAAGGTCAGTGAGCCGAATAAGGTCTGGGTGACCGATATCACCTACATCCGCACCTATGAAGGGTGGCTGTACCTGGCGGTAGTGCTGGATCTGTTCTCT
CGCCAAGTCATTGGTTGGTCAATGAAGCCCAGGATGAGCAGCGACCTAGCCATCGACGCCATGCTGATGGCCTTGTGGCGACGCAAGCCACAGCAGCAAG
TGATGATTCACTCAGACCAAGGCAGTCAGTTCAGTAGCTCAGATTGGCAAAGCTTCCTGAAGGCCAACAATGTGATCAGTAGCATGAGCCGACGGGGAAA
CTGCCACGACAATGCCGTAGCCGAGAGCTTTTTCCAGCTTTTGAAGCGGGAGCGAATCCGACGAAAGATCTACGCAACGCGTGACGAAGCCCGAAGTGAT
ATTTTCGATTACATCGAGATGTTCTATAACCCTAAACGCCGACACGGCAGCGCTATGCAGCTGTCGCCAGTAGAGTATGAGAAACGCCATTTTCTGAGCT
TGGAGAGTGTCTAGAAAACCAGGGGCGATTCA
AAGCGGTCAAGCAAGTGACCGAAAAAGGCAAACCTGTCGCCGAGGTCGCCCAGCGCCTGGGCATGTCCGTACACAGCCTTTATGCCTGGATCAAGGTCTA
CAGCAAGCCCCAAGAGCAGCGTCAGCAAGACAATGATCAGCAGGCTGAACTGCGCAAGCTGCGCGCTGAACTCAAGCGGGTGACAGAAGAGCGAGACATC
TTAAAGAAGGCCGCCGCGTACTTTGCCAAGGAGTGCGGCTGAAGTACGCCTTCATCAACAAGCACTCGACGGATTACCCAGTGCGTCGCCTTTGCCAAAC
CCTCAAGGTGCATCCCAGCGGCTACTACGCTTGGTTGGCCGAGCCTCAATCTGTCCGAGCCAAAGAAGATCAGCGCCTGCTTGGCTTGATCAAGCAGGCC
TGGTTAGAAAGCGGCGGCGTGTATGGCTATCGCAAGATTCACGATGACCTGCGAGAGCTAGGAGAGATCTGTGGGCGAAATCGAGTCGGTCGCTTGATGC
AGGCAGAGGGGCTGCGTTCGCAAACGGGCCATCGCCGTCGTACTGGGTTTTATAGCGGAAAACCAACAGCGGCATCGCCCAACCATCTGGCCCGGCAGTT
CAAGGTCAGTGAGCCGAATAAGGTCTGGGTGACCGATATCACCTACATCCGCACCTATGAAGGGTGGCTGTACCTGGCGGTAGTGCTGGATCTGTTCTCT
CGCCAAGTCATTGGTTGGTCAATGAAGCCCAGGATGAGCAGCGACCTAGCCATCGACGCCATGCTGATGGCCTTGTGGCGACGCAAGCCACAGCAGCAAG
TGATGATTCACTCAGACCAAGGCAGTCAGTTCAGTAGCTCAGATTGGCAAAGCTTCCTGAAGGCCAACAATGTGATCAGTAGCATGAGCCGACGGGGAAA
CTGCCACGACAATGCCGTAGCCGAGAGCTTTTTCCAGCTTTTGAAGCGGGAGCGAATCCGACGAAAGATCTACGCAACGCGTGACGAAGCCCGAAGTGAT
ATTTTCGATTACATCGAGATGTTCTATAACCCTAAACGCCGACACGGCAGCGCTATGCAGCTGTCGCCAGTAGAGTATGAGAAACGCCATTTTCTGAGCT
TGGAGAGTGTCTAGAAAACCAGGGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 64 | 342 | + | No |
Description : First part of the transposase
ORF sequence :
MSRQRYPEEFKIEAVKQVTEKGKPVAEVAQRLGMSVHSLYAWIKVYSKPQEQRQQDNDQQAELRKLRAELKRVTEERDILKKAAAYFAKECG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 945 bp | 314 aa | 270 | 1214 | + | No |
Description : Second part of the transposase
ORF sequence :
TQAGDRRARHLKEGRRVLCQGVRLKYAFINKHSTDYPVRRLCQTLKVHPSGYYAWLAEPQSVRAKEDQRLLGLIKQAWLESGGVYGYRKIHDDLRELGEI
CGRNRVGRLMQAEGLRSQTGHRRRTGFYSGKPTAASPNHLARQFKVSEPNKVWVTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMSSDLAIDAMLM
ALWRRKPQQQVMIHSDQGSQFSSSDWQSFLKANNVISSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRDEARSDIFDYIEMFYNPKRRHGSAMQLSP
VEYEKRHFLSLESV
CGRNRVGRLMQAEGLRSQTGHRRRTGFYSGKPTAASPNHLARQFKVSEPNKVWVTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMSSDLAIDAMLM
ALWRRKPQQQVMIHSDQGSQFSSSDWQSFLKANNVISSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRDEARSDIFDYIEMFYNPKRRHGSAMQLSP
VEYEKRHFLSLESV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 64 | 1214 | + | Yes |
Chemistry : DDE
ORF sequence :
MSRQRYPEEFKIEAVKQVTEKGKPVAEVAQRLGMSVHSLYAWIKVYSKPQEQRQQDNDQQAELRKLRAELKRVTEERDILKEGRRVLCQGVRLKYAFINK
HSTDYPVRRLCQTLKVHPSGYYAWLAEPQSVRAKEDQRLLGLIKQAWLESGGVYGYRKIHDDLRELGEICGRNRVGRLMQAEGLRSQTGHRRRTGFYSGK
PTAASPNHLARQFKVSEPNKVWVTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMSSDLAIDAMLMALWRRKPQQQVMIHSDQGSQFSSSDWQSFLK
ANNVISSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRDEARSDIFDYIEMFYNPKRRHGSAMQLSPVEYEKRHFLSLESV
HSTDYPVRRLCQTLKVHPSGYYAWLAEPQSVRAKEDQRLLGLIKQAWLESGGVYGYRKIHDDLRELGEICGRNRVGRLMQAEGLRSQTGHRRRTGFYSGK
PTAASPNHLARQFKVSEPNKVWVTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMSSDLAIDAMLMALWRRKPQQQVMIHSDQGSQFSSSDWQSFLK
ANNVISSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRDEARSDIFDYIEMFYNPKRRHGSAMQLSPVEYEKRHFLSLESV
Blast result :
Comments
ISPen2 is 96% aa similar to ISPpu22.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
An isoform of ISPen2 has been identified in Pseudomonas sp. LM15 (KJ940995) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis ISPen2 is present in 2 copies in the genome of Pseudomonas sp. LM15. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas mendocina strain LM7, Pseudomonas sp. LM8, Pseudomonas aeruginosa strain LM10, Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM14, Pseudomonas sp. LM25, Pseudomonas sp. ZM1, Pseudomonas sp. ZM2.
File updated the January 22 2015 with the information provided by Szuplewska M.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
An isoform of ISPen2 has been identified in Pseudomonas sp. LM15 (KJ940995) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis ISPen2 is present in 2 copies in the genome of Pseudomonas sp. LM15. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas mendocina strain LM7, Pseudomonas sp. LM8, Pseudomonas aeruginosa strain LM10, Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM14, Pseudomonas sp. LM25, Pseudomonas sp. ZM1, Pseudomonas sp. ZM2.
File updated the January 22 2015 with the information provided by Szuplewska M.
References
1] Vodovar,N., Vallenet,D., Cruveiller,S., Rouy,Z., Barbe,V.,Acosta,C., Cattolico,L., Jubin,C., Lajus,A., Segurens,B.,Vacherie,B., Wincker,P., Weissenbach,J., Lemaitre,B., Medigue,C.and Boccard,F. (2006) Nat. Biotechnol. 24 (6), 673-679
2] Szuplewska, M., and Bartosik, D. (2014) Direct submission.
2] Szuplewska, M., and Bartosik, D. (2014) Direct submission.