ISNwi3

  • Family IS1595
  • Group ISNwi1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007406 ND Nitrobacter winogradskyi
Nitrobacter winogradskyi Nb-255
DNA section
IS Length : 2345 bp

Ends


IR Length : 22/24

IRL : CCTACTTATATACTTGACAAAAGTAACGGGGTTCCATAAAACGGGGTCAT
IRR : CGCACTTATATACTTGACAAAAGTGCATATATTCCTAGATTCGCGTATCG

Insertion site


Left flankDirect repeatRight flankDR Length
ACGCTGTCAAGGTATATTTTTTTCCTAGGAATTTTT8

DNA sequence

CCTACTTATATACTTGACAAAAGTAACGGGGTTCCATAAAACGGGGTCATTGGAGCAAGCCGATGACCAAAGCCGTTTTTCAGAACCCTATGTTTCACGA
CGAGGACAAGGCCCGCGAAGCCCTTGAGGCGGTGCGCTGGCCCGATGGCGCTATCTGCCCTCATTGCGGCAACTCCGATCAAGACAAGCTCGCCAAGATC
GAAGGACAGAAGAAATCCCATCGTCCCGGCTTGTATTACTGCAACGAGTGCAAAGGTCAGTTCACCGTGACCGTTGGCACCGTGTTTGAGCGCTCCAAAG
TTCCCCTCACCAAATGGTGGATGGCGGCGCACATGATGAACTCAAACAAGAACGGCGTTTCGGCTCACGAAATACATCGCGCCCTTGGCGTCACCTACAA
GACCGCATGGTTCATGATGCACCGCCTGCGCGAAGCTATGGTCGTTTCCAGTGGAACCCCTATGGGCGGCTCTGGCAGCGCCATTCAGGCCGACGAAACA
TATTGGGGCAACACGTCCAAGCGCTCTAAGTCTTACCGAAAGGGCCTAAAACGCAAAGCTGCCATTGTGGCGCTTGTCGATGCAGAAACCGGTGAAGCTC
GCACCTTCCACGTTCGCCATGGCGTGAGCGCTTCTGAGGTTCGCGAAATCCTTGTTACCAACGTTGACCGTAAATCTACGCTCGTGACTGACGAAAGCAG
GCTCTACACCATAACCGGAGGTGAATTTGGCGACCATCAGACCATGAACCACAGCGGCCGCGAGTACGTGAACGCGGCTGGCTTCACTACAAACAACGTC
GAAAACTTCTTTGGCGTGTTCAAGCGTGGGATGCGCGGAACTTATACGTTCTGCGGCGAACAGCACTTGCAACGCTATCTGACGGAATTTCAATTCCGCT
ATAATAACCGCTCCGGCCTCGGAATTAGCGATGGGGAACGGACGGCGAAGGCCCTCAAAGGTATCGAGGGCAAGCGCCTGACGTACCGGCCGACTAACTA
AGATCGTTTATAAGAAGCAGGCCGCGAATCGATTCGCGGCTTGGAGGAGACGTCATGATAAGCGAGGCAATGGCGGGGGCTAGCGCGCTCAAGACCGCTT
TCGATATGGCGAAGGCTTTGAAGGACATCAGCGATGCTGCCATTCGCAATGGGGCGGTCATAGAGCTTCAAGAAAAGATTCTCTCCGCACAAGAGGCGCA
ATTTGCGTTGGTTAAGCGAGTAGACGACCTTGAAAAGCAAGTGGCTTCCTTTGAAGCAAAAGGAAGCCAAATGCAGCGATATCAGCTTAAAGATTACGGA
GCTGGATCGTTCGCCTACGAGCTGAAAGAATCGGAAGCCCGCGGCGAACCAATTCATCGCGCGTGTGCCGCCTGCTATCAACAGGGACATATCAGCATCC
TTCAATTTTCACATAATACCGAGGGGCAAGACTGGTACGAATGCAAGCGATGTAACAACATGCATCACTTCGGCACCCGAGTGCCGCGCACATACAGCCG
GACAAAAACCGACTACTTCTGAGTAGTCTTTGTTTTTTTTATTTTTTGCTTCCTTCCAACCTTCATATCCTCGTGCGGCTTTGGCTTCATGCGCACGAGA
GCGCTCATAAGACGCTTGGTCTCTTCCATCTCATTCTTTGAGTTTTTTGACATGCGCGACCTGGATTTTAATCCGAACAACCTACCGAAAGACGTTTTGG
AGGCTTTGGGTCTAATTACAGCATGTTCGGCCCATTCTGACTACGTCATCGAAAGTGGGATTGCTGGCTGCTTGGGGGTAAACATCGAATACGGATCAGC
GGTGACAACCCACATGACAACACCGCATCGAAAGGACGTGTTCCTTGCGGCAGCGGAGTTGAGAATCGATGATGTGCACATTCTTATTCATCTGGAAGAC
CTGCTAGATGAGTTAGTCGACGCCCTTAAGGTCAGGAACGGATATGTCCATAATTCTCCCGCGATAGATAGATCTACCAACGAGACGTTCATGATCCGCA
CTGAGGCGCGCGGGAGCGTTGATGTCTCACTAATCCCACTCTCGGTCGAAGTAATCCTGAGGGACGCGCAGCGCATCTACAAAGCTGGGATATCCCTCTA
CGGACTCTTGGATAAACTTGGCCTGCTTCCCAAAATCCCTGATGGGGTCAGGCCAAACCGCACCCACAAAGATAAGGCGGCGCGAAAGAAAATCCGAAAA
TCCCGCAGAGATCGCGGATAGCTCATTATCTTGCACTGTCCCTCACAGTGCACTTACCAACCTGACATTCCGGGCGCGCCAACCGACTTTCGAGCCGATA
CGCGAATCTAGGAATATATGCACTTTTGTCAAGTATATAAGTGCG
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
939 bp312 aa631001+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKAVFQNPMFHDEDKAREALEAVRWPDGAICPHCGNSDQDKLAKIEGQKKSHRPGLYYCNECKGQFTVTVGTVFERSKVPLTKWWMAAHMMNSNKNGVS
AHEIHRALGVTYKTAWFMMHRLREAMVVSSGTPMGGSGSAIQADETYWGNTSKRSKSYRKGLKRKAAIVALVDAETGEARTFHVRHGVSASEVREILVTN
VDRKSTLVTDESRLYTITGGEFGDHQTMNHSGREYVNAAGFTTNNVENFFGVFKRGMRGTYTFCGEQHLQRYLTEFQFRYNNRSGLGISDGERTAKALKG
IEGKRLTYRPTN

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
468 bp155 aa10551522+No
ORF function : Passenger Gene
Annotation : Phage related proteinDescription :

ORF sequence :

MISEAMAGASALKTAFDMAKALKDISDAAIRNGAVIELQEKILSAQEAQFALVKRVDDLEKQVASFEAKGSQMQRYQLKDYGAGSFAYELKESEARGEPI
HRACAACYQQGHISILQFSHNTEGQDWYECKRCNNMHHFGTRVPRTYSRTKTDYF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
633 bp210 aa15892221+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRTRALIRRLVSSISFFEFFDMRDLDFNPNNLPKDVLEALGLITACSAHSDYVIESGIAGCLGVNIEYGSAVTTHMTTPHRKDVFLAAAELRIDDVHILI
HLEDLLDELVDALKVRNGYVHNSPAIDRSTNETFMIRTEARGSVDVSLIPLSVEVILRDAQRIYKAGISLYGLLDKLGLLPKIPDGVRPNRTHKDKAARK
KIRKSRRDRG

 

Blast result :
Comments
ISNwi3 is 49% aa similar to ISPepr2. The second and the third ORF are passenger gene.
References
1] Starkenburg,S.R., Chain,P.S., Sayavedra-Soto,L.A., Hauser,L., Land,M.L., Larimer,F.W., Malfatti,S.A., Klotz,M.G., Bottomley,P.J., Arp,D.J. and Hickey,W.J.(2006) Appl. Environ. Microbiol. 72 (3), 2050-2063