ISNwi2

  • Family IS1595
  • Group ISNwi1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007406 ND Nitrobacter winogradskyi
Nitrobacter winogradskyi Nb-255
DNA section
IS Length : 1750 bp

Ends


IR Length : 21/23

IRL : GATATATACCCTATGGACATGTAGAACATACCGTGATATAGAATGGGCAT
IRR : GATATATACCCTTAGGACATGTAATATGAATCGTGTTATTCGTCCGTCTG

Insertion site


Left flankDirect repeatRight flankDR Length
GCTTAGGCCAGCCCGACGCAGCGAA5

DNA sequence

GATATATACCCTATGGACATGTAGAACATACCGTGATATAGAATGGGCATTCTCCTAGGAGGGAGAGTGCAATGGCTCAAAAGCCGTCGAAAATTCGCGA
ATTTCAGAAGCGGTTCCCTGATGAAGAGGCTTGCCTGACGCATTTGATGCGCACCCGGTTTGGCGAACGTCTGACCTGCTTCAAGTGTCAGAAGCAGGCG
ACCTATTACCGGGTCAAAAACCGCCGCTCCTTTGAGTGCGAGCATTGCGGCTATCAGGTGTATCCGACTGCCGGAACGCCGTTCGAAAAGACACGCACCA
GCCTGCGGGATTGGTTCTATGTCATGTTTCTGTTCTGCGAGACGCGGAACGGCGTGGCCGCGAAAGAAGTGCAGCGTCAGCTAGGCGTGACCTACAAGAC
CGCTTGGCGCATGTGCCATCTTATCCGGCTCTATATGGGCTACGTCGATGGCGACTATCCAATGGGCGGGCCCGGTAAGACCGTTGAGGCGGACGAAACG
TTCATCGGCGGCAAGGACAAGGCTGGCAAAGACGACAAAGCAATTGTCATGGGGATGATCGAGCGTGGTGGCGATGTCGTTACGCGCGTCGTCCATGCTC
GTACAAAGGCGCATATCTTGCCGCACATCGTTCGGTTTGTGAAAGAAGGTTCGCGCGTCCACACGGACGAGGCGTCAAACTTCAAGCTTCTCAAGGAGAA
GTATGGCTACGATCACGAAGCGGTTGATCACTCTGCGAAGGAGTACGTTCGCGGTGACGTACACACTAACTCAATCGAGTCGTTCTGGTCTCAGGTGAAG
CGTGGGATCAACGGGACCTATGTTTGGGTTTCGAAAAAGCACTTGCAGCTTTACCTTCGCGAGTTCGAGTATCGCCATAACTTGCGGAAGCAACCCGGCC
TGATGTTCGAGCTTCTTTTGCAGGCGTTCCCGAAGGTACGGGTTGAGTGACCATAGCCTGCAAAAGCTGATCGAAGCGCTTTCTCACCTTACTGTCTGAA
GACATGGCGGTCCTCTTGCAAAAAGGAATAAATGTTTCCTGTCGCGGCCTCATTCTCGCGATACGCGATGAATACCGAATAGCGCTGCTTCACTCGACCC
TTCTTAACCGCCCTCACTTCGACCAGATTTCCCGTACCGGGTATAGGCGATTTGTTTGCGGTGCCATCCCCCCAAAAATGGGGGCCATGGGCCGCTTGCG
CAATCGTTGCGATGGGTACGCGCACATGCTCACCTTCTGAAAACTTCACCGCGTCAACTCGCAGCAGCAACTGCGCTGGCTTATTAAACGACCATTGAAG
GTGTTCGTACAGGACTAGTTCCAGGGAGTGACCGGTCACAGGGCCAATAAATCTAACAGAAAAATATGGGGTTTGGACCTCGTATCCGTCTTTTACATCG
CTGGCCGGATGGAACACGACATTGAATACTCGCCGCCGATCTGCCCACCACCTCCGAAGCTGTGAGTTATCGTTGTAGAGGTCAGCATAAAATGTGCCGA
GTCCGAATGCGGCAATCGTCGCTAGGATGGCGATCCACGGCACGTCTACCCACCACGGCCGGAATTGAAGCCATTGTTGGCTGCAAAACCAAGCCGCCCC
AAACAATAAAATAAGCCCTGGTACGGGCAGATATTCCCTAAAAGGGAATTGGCTCTTGCGCATCCAAACGTCTCCCACGCCCGTCTAGGGCTAGTTGTGG
CAGACGGACGAATAACACGATTCATATTACATGTCCTAAGGGTATATATC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
879 bp292 aa72950+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAQKPSKIREFQKRFPDEEACLTHLMRTRFGERLTCFKCQKQATYYRVKNRRSFECEHCGYQVYPTAGTPFEKTRTSLRDWFYVMFLFCETRNGVAAKEV
QRQLGVTYKTAWRMCHLIRLYMGYVDGDYPMGGPGKTVEADETFIGGKDKAGKDDKAIVMGMIERGGDVVTRVVHARTKAHILPHIVRFVKEGSRVHTDE
ASNFKLLKEKYGYDHEAVDHSAKEYVRGDVHTNSIESFWSQVKRGINGTYVWVSKKHLQLYLREFEYRHNLRKQPGLMFELLLQAFPKVRVE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
675 bp224 aa1663989-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRKSQFPFREYLPVPGLILLFGAAWFCSQQWLQFRPWWVDVPWIAILATIAAFGLGTFYADLYNDNSQLRRWWADRRRVFNVVFHPASDVKDGYEVQTPY
FSVRFIGPVTGHSLELVLYEHLQWSFNKPAQLLLRVDAVKFSEGEHVRVPIATIAQAAHGPHFWGDGTANKSPIPGTGNLVEVRAVKKGRVKQRYSVFIA
YRENEAATGNIYSFLQEDRHVFRQ

 

Blast result :
Comments
ISNwi2 is 61% aa similar to ISSpo3. The second ORF is on complementary strand, this is a passenger gene.
References
1] Starkenburg,S.R., Chain,P.S., Sayavedra-Soto,L.A., Hauser,L., Land,M.L., Larimer,F.W., Malfatti,S.A., Klotz,M.G., Bottomley,P.J., Arp,D.J. and Hickey,W.J. (2006) Appl. Environ. Microbiol. 72 (3), 2050-2063