ISDge2
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000359 | ND | Deinococcus geothermalis | Deinococcus geothermalis DSM 11300 |
DNA section
IS Length : 748 bp
Ends
IR Length : 19/21
IRL : GGTAGTGGCTGCGACGACGTGATGTAATGGAGGGGTGCCCGAGTGCCCAA
IRR : GGTAGTGGCTGCATCAACGTGACAGGTTGTAGCGATGGAAGGCGAGGGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCCAGAAAGTCTCT | TCTGGACA | ACCAAGAGGACCG | 8 |
DNA sequence
GGTAGTGGCTGCGACGACGTGATGTAATGGAGGGGTGCCCGAGTGCCCAACCTGTCAGAGCATCCAGACGGTCAAGAACGGAAAGGCCAAAAACGGCACC
CAGACGTACTTGTGTAAGGTCTGTGGTCGTCGCTTCCACCCCAACGCCAGACCTGTGGCCCACAGTGAAGCGACCAAGGAGCAGATTCTCCAGGCGGTTC
ATGAGCGAATGAGCCTCAGAGGCGTACAGCGCGTGTTTGGCGTCCACCGCAACACCGTCATCCGGTGGATAAAAAGGGGGCCTCCGAAGTGAGGCAGACC
GTACCGGTCTGCCTCACACCTCCCGAAGAAGTGGTGGTTGAGCTGGATGAATTGTGGACCTTCGTGGGCAAGAAAAAGCAGGCGAGGTGGCTCTGGATTG
CCCTGGAGCGCAGCACCCGAAAGGTGCTGGCTTGGGTTCTGGGCGACCGGAGTGAGCAAACCGCGTTCAAGCTCTGGGACCGCTTGCCGCTGTCCCCAGA
ACAGCGCCTGAAGGGCACGTTTTGCACGGACCTGTGGCGTGCTTACGACGAGCCACTCCTGGGGGTGAAGCGGCTCACCCGCAAGGGGGAAACGAACCAC
GTCGAACGGCTCAACTGCACCCTCAGACAGCGGCTAGGTCGGCTGGTCCGCAAGTCGTTGTCCTTCTCAAAGTCCGACGAGATGCTCGAAGCGAGCCTGA
CCCTCGCCTTCCATCGCTACAACCTGTCACGTTGATGCAGCCACTACC
CAGACGTACTTGTGTAAGGTCTGTGGTCGTCGCTTCCACCCCAACGCCAGACCTGTGGCCCACAGTGAAGCGACCAAGGAGCAGATTCTCCAGGCGGTTC
ATGAGCGAATGAGCCTCAGAGGCGTACAGCGCGTGTTTGGCGTCCACCGCAACACCGTCATCCGGTGGATAAAAAGGGGGCCTCCGAAGTGAGGCAGACC
GTACCGGTCTGCCTCACACCTCCCGAAGAAGTGGTGGTTGAGCTGGATGAATTGTGGACCTTCGTGGGCAAGAAAAAGCAGGCGAGGTGGCTCTGGATTG
CCCTGGAGCGCAGCACCCGAAAGGTGCTGGCTTGGGTTCTGGGCGACCGGAGTGAGCAAACCGCGTTCAAGCTCTGGGACCGCTTGCCGCTGTCCCCAGA
ACAGCGCCTGAAGGGCACGTTTTGCACGGACCTGTGGCGTGCTTACGACGAGCCACTCCTGGGGGTGAAGCGGCTCACCCGCAAGGGGGAAACGAACCAC
GTCGAACGGCTCAACTGCACCCTCAGACAGCGGCTAGGTCGGCTGGTCCGCAAGTCGTTGTCCTTCTCAAAGTCCGACGAGATGCTCGAAGCGAGCCTGA
CCCTCGCCTTCCATCGCTACAACCTGTCACGTTGATGCAGCCACTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 258 bp | 85 aa | 35 | 292 | + | No |
Description : First part of the transposase
ORF sequence :
VPECPTCQSIQTVKNGKAKNGTQTYLCKVCGRRFHPNARPVAHSEATKEQILQAVHERMSLRGVQRVFGVHRNTVIRWIKRGPPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 531 bp | 176 aa | 205 | 735 | + | No |
Description : Second part of the transposase
ORF sequence :
ANEPQRRTARVWRPPQHRHPVDKKGASEVRQTVPVCLTPPEEVVVELDELWTFVGKKKQARWLWIALERSTRKVLAWVLGDRSEQTAFKLWDRLPLSPEQ
RLKGTFCTDLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQRLGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR
RLKGTFCTDLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQRLGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 701 bp | 233 aa | 35 | 735 | + | Yes |
Chemistry : DDE
ORF sequence :
VPECPTCQSIQTVKNGKAKNGTQTYLCKVCGRRFHPNARPVAHSEATKEQILQAVHERMSLRGVQRVFGVHRNTVIRWIKKGASEVRQTVPVCLTPPEEV
VVELDELWTFVGKKKQARWLWIALERSTRKVLAWVLGDRSEQTAFKLWDRLPLSPEQRLKGTFCTDLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQR
LGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR
VVELDELWTFVGKKKQARWLWIALERSTRKVLAWVLGDRSEQTAFKLWDRLPLSPEQRLKGTFCTDLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQR
LGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR
Blast result :
Comments
ISDge2 is 62% (ORF A) and 57% (ORF B) aa similar to ISSru3. The third ORF is a putative ORF AB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Saunders,E., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Daly,M.J., Fredrickson,J.K., Makarova,K.S., Gaidamakova,E.K., Zhai,M. and Richardson,P. US DOE Joint Genome Institute. (2006) Direct submission GenBank.