ISSod9

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) TnSod9
Accession numberTranspositionOriginHost
NC_004349 ND Shewanella oneidensis
Shewanella oneidensis MR-1
DNA section
IS Length : 4412 bp

Ends


IR Length : 39

IRL : GGGGTCGCCTCAGAAAACGGAAAAAATCGTACGCTAAGCATGCGCAGAGG
IRR : GGGGTCGCCTCAGAAAACGGAAAAAATCGTACGCTAAGCCGGTGATAGAG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGGGTCGCCTCAGAAAACGGAAAAAATCGTACGCTAAGCATGCGCAGAGGCAGGGAGCCAGCGGTATAGCGTAGGAACCGACACGCCAAGATTCTTAGCC
ACATCCTTGGGGGGGACTCCACTGGCTAATAGCTTTTTGGCTGATTCGATCTTGCTGTCGGTCATCTTCGGCTTGCGACCGCCTTTACGACCGAGTTGTC
TGGCAACGTCCAACCCCGCACGGGTTCTTTCGACGATCAAGTCTCGTTCCATCTCTGCGAGGCTTGCCATGACATGAAAAAAGAATCGGCCCGATGGCGT
GCCGGTATCGATAGAATCGGTGAGACTCTTAAACTGGACGTTTTGTTTGTGCAGATCGCTGACCAGCTCTACCAGTTGTTTGACTGACCGGCCCAATCTA
TCCAGCTTCCAAACAACCAATGTATCCCCTTCGCGCAGTATTTCAAGGGCTTTGCTCAAACCAAGCCTGTCAGCCCGAGTACCACTGATTGTATCTTCAA
ACACTTTTTCGCACCCCGCTTTAAGCAAGGCTTCTCGCTGAAGTTCTAGGTGCTGATCTTGTGTTGATACTCTTGCATAACCGATCAGCATAAATCATGA
CCTTCATTTTTATCTACATCATATCTAACCGCTGCCAGCTGCGATAATAATGTTGGCAGCGCCGTTTGTACCGTATCCCAAACCACATCAAGGTTTATAT
CGAAATAACCATGAGCAATGCGATTGCGCATTCCACGCATACTACGCCATGGAACGTCTGAATGGGCAATGACAAACTCAGAATAACCGTCCATCACCTT
AGTTGATGCTTCACCAATTACGATCAGACTCATGATGACAGCCTGTTGGGTACGTTTATCCACTAAAAATTCATCTTTATCCAACCCTTCAACAAAATAA
CACGCATCGGAAGCGGCTTGGTGCATGTGGTCAAGATAATCAGGTAAACGGTTCACCTTCATACAGGTCGTGCCTCCGCGAGTACTTGGGCTCGAAATTT
CGGTGGTAAATCGCCAGGGGTCAGTAGATCAACTTGAAGGCCGAGCAGCGATTCAAGTTCATCTTGTAAACCTCCCAAGTCAAAAAGTGTCGCTCCAGGG
AGGGCATCAACTAATAAGTCCAGATCACTACCATCGAGATCTGTCCCATCGAGCACTGAACCAAAAACACGTGGATTGGTTACGCGAAAACGACTCGCGG
TCTCACGAATAACGCTTCGCTTAAGAGCGAGCACTGCGGATGGTCTCATAATGGCTATTTCCTTATTATCAAAACTCGTTGAAATGATATGCAACTGAGA
ATAATATTTCAAGAATGATTTTTGAGAATCATAACCCCTTGATCTGCTGTTGTGTCCGAGAGCTCTTGTCGTTGCTCTTACAAACCTACGTTTTCAAGAA
GAAGGAAACCGCATGCCTCGCCGCTCAATCCTATCCGCCGCAGAGCGAGACAGCCTGCTGGTGTTACCCGATACCCAAGACGAACTGATCCGCCACTATA
CGTTTAGCGAACCCGATCTATCACTGATACGGCAGCGGCGCGGTGATGCTAACCGCTTGGGGATCGCGGTACAGTTATGCTTGCTGCGCTTCCCAGGCCA
GGGCTTGTTGCCCGACGCTACGGTGCCAATGCCTCTGCTGCAATGGATAGGACAACAGTTACAGCTCGATCCTGTATGTTGGCCGCAGTATGCCGAGCGA
GAGGAGACACGGCGCGAGCACTTGCTCGAACTGCGGGTGTATCTTGGCATGGAACCATTTAGTCAAGTGCACCATCGGCAGGCTGTCCATACCACGACCG
AACTGGCCTTGCAGACTGACAAGGGCATAGTGTTGGCCAACAGCGTAGTCGAGACGCTGCGTCATAAACACATCATTTTGCCAACGTTAGATGTTGTCGA
GCGCGTCTGTGCCGAGGCTCTAACCCGTGCAAACCGGCGTATCTACGACACCTTGACCGAACCACTATCAGGCTCGCACCGCCACCGGCTCGATGATCTG
CTCAAGCTTCGGGACAACAACAAAACGACTACGCTGGCTTGGCTTCGCCTGTCTCCGGTCAAACCCAATTCGCGGCACATGCTTGAGCACATCGAACGAC
TCAAGGTATGGCAGGCGCTTGATCTTCCTATTGGTGTCGATCGTCTGATCCACCAAAACCGATTGCTCAAGATCGCCCGAGAAGGCGGACAGATGACCCC
CGCCGATTTGGCCAAGTTCGAGCCACAACGCCGCTATGCGACTTTGGTTGCGCTAGCCATCGAGGGGATGGCCACTGTTACAGATGAAATTATTGATCTG
CATGACCGCATCATGGGCAAGCTGTTCAATGATGCCAAGAAGCGACATCAGAAACAGTTTCAGGCATCGGGGAAGGCTATCAATGCCAAGGTGCGCCTGT
TCGGCCGTATCGGCCAAGTGTTGATCGACGCTAAGCAAGCGGGTGATGATCCGTTTGCTGCTATCGAGGGCGTCATATCCTGGGAGGCCTTTGCCAAGAG
CGTGACAGAGGCACAATCGCTCGCGCAGCCCGAGGAATTCGATTTCCTGTACCGTCTCGGTGAGAGCTACGCCACACTACGCCGTTACGCACCGACCTTC
CTCACCGCGCTAAAGTTGCGGGCCGCACCGGCTGCCAAAGGTGTATTGGAGGCCATCGAAGTACTGCGCAGCATGAACAACGACAACGCCCGAAAAGTAC
CTGCAGATGCGCCAATTGATTTCATCAAGCCGCGTTGGCAGAAGCTGGTGATAACCGATACCGGCATCGACCGGCGTTACTACGAGCTATGCGCGCTGTC
GGAGATGAGGAACGCATTGCGTTCCGGTGACATCTGGGTACAGGGATCGCGCCAGTTCAAGGACTTCGAGGACTACCTTGTACCACCCGCGAAATTCGTC
AGTCTCAAGCAGACCAATCAATTGCCGCTGGCCGTAGCCACCGACTGTGAGCAGTATCTGAATGAACGGCTGACGCAATTGGAAACGCAGCTCGCCACCG
TCAACAGCATGGCGCAGGCTAACGAATTACCGGATGCCATCATCACGGCCTCTGGCCTAAAGATAACGCCGCTGGATGCAGTAGTACCAGATACCGCGCA
GCGCCTTATCGATCAGGCTGCTAGGATCCTGCCGCACGTCAAGATCACTGAGTTACTGCTTGAAGTGGACGAATGGACGGGCTTTACCAGGCACTTCGCA
CACCTGAAATCTGGTGTTCTGGCCAAGGACAAGAACCTGCTACTGACGACTATCCTTGCCGATGCGATCAATCTGGGTCTGACCAAGATGGCAGAATCGT
GTCCAGGAACGACTTATGCCAAGCTCGCTTGGCTGCAAGCCTGGCATATCCGTGACGAAACCTATGGCGCGGCCTTGTCCGAGCTGGTCAATGCGCAGTA
CCGGCATCCGTTTGCCGAACATTGGGGCGATGGTTCTACATCCTCCTCTGACGGCCAGAATTTTCGCACCGGCAACAAGGCGGAGAGTACGGGGCACATC
AATCCGAAATATGGCAGTAGTCCAGGACGGACCTTTTACACGCATATTTCCGACCAGTACGCACCATTTCATACTAAGGTGGTCAATGTCGGTGTGCGCG
ACTCAACCTACGTGCTCGACGGCCTGCTCTACCATGAATCTGACTTACGGATCGAAGAGCACTACACCGACACGGCTGGCTTCACCGATCACGTTTTCGC
GTTGATGCACCTGTTGGGGTTTCGCTTCGCACCGCGTATCCGCGACTTGGGTGAGACCAAGCTCTACATTCCAAAGCGCGATGTCACCTACGAGGGATTG
AAATCAATGATTGGCGGGACGCTCAACATCAAGCTAATCCGTACACATTGGGACGAGATCTTGCGGCTGGCTACCTCGATAAAACAGGGAACGGTGACGG
CCTCACTCATGCTGAGGAAGCTTGGCAGCTACCCACGTCAGAATGGCTTGGCGGTCGCCTTGCGCGAATTGGGGCGTATCGAGCGCACGCTGTTCATTCT
GGATTGGCTGCAAAGTGTCGAGCTCCGCCGCCGGGTACATGCCGGACTGAATAAGGGGGAGGCTCGTAACGCCTTGGCTCGTGCCGTGTTTTTCAATCGA
CTGGGGGAGATCCGTGACCGCAGTTTTGAGCAACAGCGTTACCGGGCAAGTGGCCTTAACTTGGTGACTGCCGCCATCGTGCTATGGAACACCGTCTATC
TGGAAAGGGTGGCACATGGGTTACGTGCCAAGGGACATGCCGTCGATGAAGAGTTATTGCAGTACCTATCGCCGCTAGGCTGGGAGCACATCAACCTGAC
AGGGGATTATCTGTGGCGCAGCAGCGCCAAGATAGGTTCAGGTAAATTCAGGCCTCTACGACCTCTATCACCGGCTTAGCGTACGATTTTTTCCGTTTTC
TGAGGCGACCCC
Protein section
ORF number : 4

 

ORF 1
LengthBeginEndStrandFusion ORF
557 bp185 aa59034-No
ORF function : Passenger Gene
Annotation : DNA-invertaseDescription :

ORF sequence :

MLIGYARVSTQDQHLELQREALLKAGCEKVFEDTISGTRADRLGLSKALEILREGDTLVVWKLDRLGRSVKQLVELVSDLHKQNVQFKSLTDSIDTGTPS
GRFFFHVMASLAEMERDLIVERTRAGLDVARQLGRKGGRKPKMTDSKIESAKKLLASGVPPKDVAKNLGVSVPTLYRWLPASAHA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
378 bp125 aa962585-No
ORF function : Passenger Gene
Annotation : hypothetical proteinDescription :

ORF sequence :

MKVNRLPDYLDHMHQAASDACYFVEGLDKDEFLVDKRTQQAVIMSLIVIGEASTKVMDGYSEFVIAHSDVPWRSMRGMRNRIAHGYFDINLDVVWDTVQT
ALPTLLSQLAAVRYDVDKNEGHDLC

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
291 bp96 aa1249959-No
ORF function : Passenger Gene
Annotation : nucleotidyltransferase domain-containing proteinDescription :

ORF sequence :

MRPSAVLALKRSVIRETASRFRVTNPRVFGSVLDGTDLDGSDLDLLVDALPGATLFDLGGLQDELESLLGLQVDLLTPGDLPPKFRAQVLAEARPV

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
2967 bp988 aa14134379+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRRSILSAAERDSLLVLPDTQDELIRHYTFSEPDLSLIRQRRGDANRLGIAVQLCLLRFPGQGLLPDATVPMPLLQWIGQQLQLDPVCWPQYAEREETR
REHLLELRVYLGMEPFSQVHHRQAVHTTTELALQTDKGIVLANSVVETLRHKHIILPTLDVVERVCAEALTRANRRIYDTLTEPLSGSHRHRLDDLLKLR
DNNKTTTLAWLRLSPVKPNSRHMLEHIERLKVWQALDLPIGVDRLIHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVALAIEGMATVTDEIIDLHDRI
MGKLFNDAKKRHQKQFQASGKAINAKVRLFGRIGQVLIDAKQAGDDPFAAIEGVISWEAFAKSVTEAQSLAQPEEFDFLYRLGESYATLRRYAPTFLTAL
KLRAAPAAKGVLEAIEVLRSMNNDNARKVPADAPIDFIKPRWQKLVITDTGIDRRYYELCALSEMRNALRSGDIWVQGSRQFKDFEDYLVPPAKFVSLKQ
TNQLPLAVATDCEQYLNERLTQLETQLATVNSMAQANELPDAIITASGLKITPLDAVVPDTAQRLIDQAARILPHVKITELLLEVDEWTGFTRHFAHLKS
GVLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHIRDETYGAALSELVNAQYRHPFAEHWGDGSTSSSDGQNFRTGNKAESTGHINPKY
GSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYIPKRDVTYEGLKSMI
GGTLNIKLIRTHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEI
RDRSFEQQRYRASGLNLVTAAIVLWNTVYLERVAHGLRAKGHAVDEELLQYLSPLGWEHINLTGDYLWRSSAKIGSGKFRPLRPLSPA

 

Blast result :
Comments
There are two copies of this transposon on the plasmid of this strain. The transposase is the ORF4, 48% aa similar to ISXc5.

The others ORF :
ORF1 : DNA-invertase
ORF2 : conserved hypothetical protein
ORF3 : nucleotidyltransferase domain protein
References
1] Heidelberg,J., Paulsen,I., Nelson,K., Gaidos,E., Nelson,W., Read,T., Eisen,J., Seshadri,R., Ward,N., Methe,B., Clayton,R., Meyer,T., Tsapin,A., Scott,J., Beanan,M., Brinkac,L., Daugherty,S., DeBoy,R., Dodson,R., Durkin,A., Haft,D., Kolonay,J., Madupu,R., Peterson,J., Umayam,L., White,O., Wolf,A., Vamathevan,J., Weidman,J., Impraim,M., Lee,K., Berry,K., Lee,C., Mueller,J.,Khouri,H., Gill,J., Utterback,T., McDonald,L., Feldblyum,T., Smith,H., Venter,J., Nealson,K. and Fraser,C. (2002) Nat. Biotechnol. 20 (11), 1118-1123