ISVisp3
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAMR01000005 | ND | Vibrio splendidus | Vibrio splendidus 12B01 |
DNA section
IS Length : 1499 bp
Ends
IR Length : 16/26
IRL : TGATTGGAACACAGTAAACAAAAGTACATTTTTCAGCAGTTTCATTCGAC
IRR : TGATTGGATTGCTAGTATGAAGCTTTGGAGTTACCTCTTATTGTCTTTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGCTAGAAG | TCAAACACAA | 0 |
DNA sequence
TGATTGGAACACAGTAAACAAAAGTACATTTTTCAGCAGTTTCATTCGACTTACTCAATTTTATAAGAAAATAGCAGCACAGTATCTGTGCATTATGTTG
ATCAATCATAATCATAATGCTTTGTAAATTCCGTCTTTGATTTGTCTAGAAAATGTAACCACCCCATAAGTGATCTGCACCAGCAGTTTTGGACACCGAG
TTAAGTGAGTACAATCACTAGCGAGGTGAACCATGACAAGCAAGAAAAAACGTATTATCCATTCCCCTGAATTTAAAGCAGAAGCCCTTAAGCTAGCAGA
GAAAGTGGGCGTAGCTGCGGCAGCGAGGCAACTGCCGTTACACGAATCCCAGATCTATGGTTGGCGTAAGGCCGCTAAGGGCGACACCAGTGCCAGTCAG
CGGGAAAAAGATCTAGCCGCTGAAGTTGCCAAACTCAAAAAACAATTGGCTGAGCAAGTTGAAGAGCTAGATATAGTAAAAAAGGCCGCCACCTACTTCG
CGAAAAACCTAAAGTAGATTGCTATGAATTTATGCTCGAACACCTGCTGTGCTTCAGTGTTGTCCACATGGCTAAGGTGTTCGGCGTTTCACGAAGTGGG
TTTTATTACTGGATTAAGCATCGCCACAAGGCCAGCCAACGCGAGGTAACTCGCCAAAAGCTTGATACGAAGGTCAAAAAGGCTTTTGACAATAGCAAAG
GTCGTGATGGCTCAAGGCGCATCCAGAAAGAGCTGGCTGAGAACGGTGACAGCCGTAATGTTAAAACCATTGCCTCCAGTATGAAGCGGCAGGATTTAAC
GCCGAAAGAGGCACGTAAGTTTAAGTGTACTACGGATAGCAAACATAAAGTGCCAGTTGCTCCGAACCTGCTGGCTCAGGATTTTAACGCGGAGGCTCCG
AATCAAAAGTGGGCGGGAGACATCACCTATGTTGCAACAAGCGAAGGCTGGCTGTATTTTGCAGCAATCATTGACCTTTATTCAAGGCAAGTAGTCGGCT
GGTCTATGGATACCAGAATGACGGCAAATCTGGTCTGCGATGCGTTATCAATGGCCTTGTTCCGTCGTGGATTCCCTAAGCAAGTTATCGTTCATAGTGA
CCGAGGTAGTCAGTACTGCTCAAAAGATTATCGAGACATCATAACCGCTTATAATCTAGAGCAAAGTATGAGTAGAAAAGGAAACTGCTGGGACAATGCT
TGTGTTGAGAGCTTCTTCCATTCAATGAAAGTTGGGGCGATCCAGTACGAGCCGCTCATGACGCGAGACGAGATGCGCCAAACGATCTTTGAATACATAG
AGCTTGATTATAATCGGACAAGAAGGCACAGTGCTCTTGGGTATCTAAGCCCAGTTAACTTTGAAAATCAAAATGTCGCTTAATGAGGTGTCCAGTCTGG
CTGGAGCAGATCAAATTACTCACTTTGATTAAAAGTTCTGAATCCATAATCAAAGACAATAAGAGGTAACTCCAAAGCTTCATACTAGCAATCCAATCA
ATCAATCATAATCATAATGCTTTGTAAATTCCGTCTTTGATTTGTCTAGAAAATGTAACCACCCCATAAGTGATCTGCACCAGCAGTTTTGGACACCGAG
TTAAGTGAGTACAATCACTAGCGAGGTGAACCATGACAAGCAAGAAAAAACGTATTATCCATTCCCCTGAATTTAAAGCAGAAGCCCTTAAGCTAGCAGA
GAAAGTGGGCGTAGCTGCGGCAGCGAGGCAACTGCCGTTACACGAATCCCAGATCTATGGTTGGCGTAAGGCCGCTAAGGGCGACACCAGTGCCAGTCAG
CGGGAAAAAGATCTAGCCGCTGAAGTTGCCAAACTCAAAAAACAATTGGCTGAGCAAGTTGAAGAGCTAGATATAGTAAAAAAGGCCGCCACCTACTTCG
CGAAAAACCTAAAGTAGATTGCTATGAATTTATGCTCGAACACCTGCTGTGCTTCAGTGTTGTCCACATGGCTAAGGTGTTCGGCGTTTCACGAAGTGGG
TTTTATTACTGGATTAAGCATCGCCACAAGGCCAGCCAACGCGAGGTAACTCGCCAAAAGCTTGATACGAAGGTCAAAAAGGCTTTTGACAATAGCAAAG
GTCGTGATGGCTCAAGGCGCATCCAGAAAGAGCTGGCTGAGAACGGTGACAGCCGTAATGTTAAAACCATTGCCTCCAGTATGAAGCGGCAGGATTTAAC
GCCGAAAGAGGCACGTAAGTTTAAGTGTACTACGGATAGCAAACATAAAGTGCCAGTTGCTCCGAACCTGCTGGCTCAGGATTTTAACGCGGAGGCTCCG
AATCAAAAGTGGGCGGGAGACATCACCTATGTTGCAACAAGCGAAGGCTGGCTGTATTTTGCAGCAATCATTGACCTTTATTCAAGGCAAGTAGTCGGCT
GGTCTATGGATACCAGAATGACGGCAAATCTGGTCTGCGATGCGTTATCAATGGCCTTGTTCCGTCGTGGATTCCCTAAGCAAGTTATCGTTCATAGTGA
CCGAGGTAGTCAGTACTGCTCAAAAGATTATCGAGACATCATAACCGCTTATAATCTAGAGCAAAGTATGAGTAGAAAAGGAAACTGCTGGGACAATGCT
TGTGTTGAGAGCTTCTTCCATTCAATGAAAGTTGGGGCGATCCAGTACGAGCCGCTCATGACGCGAGACGAGATGCGCCAAACGATCTTTGAATACATAG
AGCTTGATTATAATCGGACAAGAAGGCACAGTGCTCTTGGGTATCTAAGCCCAGTTAACTTTGAAAATCAAAATGTCGCTTAATGAGGTGTCCAGTCTGG
CTGGAGCAGATCAAATTACTCACTTTGATTAAAAGTTCTGAATCCATAATCAAAGACAATAAGAGGTAACTCCAAAGCTTCATACTAGCAATCCAATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 233 | 517 | + | No |
Description : First part of the transposase
ORF sequence :
MTSKKKRIIHSPEFKAEALKLAEKVGVAAAARQLPLHESQIYGWRKAAKGDTSASQREKDLAAEVAKLKKQLAEQVEELDIVKKAATYFAKNLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 921 bp | 306 aa | 463 | 1383 | + | No |
Description : Second part of the transposase
ORF sequence :
RARYSKKGRHLLREKPKVDCYEFMLEHLLCFSVVHMAKVFGVSRSGFYYWIKHRHKASQREVTRQKLDTKVKKAFDNSKGRDGSRRIQKELAENGDSRNV
KTIASSMKRQDLTPKEARKFKCTTDSKHKVPVAPNLLAQDFNAEAPNQKWAGDITYVATSEGWLYFAAIIDLYSRQVVGWSMDTRMTANLVCDALSMALF
RRGFPKQVIVHSDRGSQYCSKDYRDIITAYNLEQSMSRKGNCWDNACVESFFHSMKVGAIQYEPLMTRDEMRQTIFEYIELDYNRTRRHSALGYLSPVNF
ENQNVA
KTIASSMKRQDLTPKEARKFKCTTDSKHKVPVAPNLLAQDFNAEAPNQKWAGDITYVATSEGWLYFAAIIDLYSRQVVGWSMDTRMTANLVCDALSMALF
RRGFPKQVIVHSDRGSQYCSKDYRDIITAYNLEQSMSRKGNCWDNACVESFFHSMKVGAIQYEPLMTRDEMRQTIFEYIELDYNRTRRHSALGYLSPVNF
ENQNVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 233 | 1383 | + | Yes |
Chemistry : DDE
ORF sequence :
MTSKKKRIIHSPEFKAEALKLAEKVGVAAAARQLPLHESQIYGWRKAAKGDTSASQREKDLAAEVAKLKKQLAEQVEELDIVKKGRHLLREKPKVDCYEF
MLEHLLCFSVVHMAKVFGVSRSGFYYWIKHRHKASQREVTRQKLDTKVKKAFDNSKGRDGSRRIQKELAENGDSRNVKTIASSMKRQDLTPKEARKFKCT
TDSKHKVPVAPNLLAQDFNAEAPNQKWAGDITYVATSEGWLYFAAIIDLYSRQVVGWSMDTRMTANLVCDALSMALFRRGFPKQVIVHSDRGSQYCSKDY
RDIITAYNLEQSMSRKGNCWDNACVESFFHSMKVGAIQYEPLMTRDEMRQTIFEYIELDYNRTRRHSALGYLSPVNFENQNVA
MLEHLLCFSVVHMAKVFGVSRSGFYYWIKHRHKASQREVTRQKLDTKVKKAFDNSKGRDGSRRIQKELAENGDSRNVKTIASSMKRQDLTPKEARKFKCT
TDSKHKVPVAPNLLAQDFNAEAPNQKWAGDITYVATSEGWLYFAAIIDLYSRQVVGWSMDTRMTANLVCDALSMALFRRGFPKQVIVHSDRGSQYCSKDY
RDIITAYNLEQSMSRKGNCWDNACVESFFHSMKVGAIQYEPLMTRDEMRQTIFEYIELDYNRTRRHSALGYLSPVNFENQNVA
Blast result :
Comments
Genome in progress.
ISVisp3 is 95% (ORFA) and 94%% (ORFB) aa similar to ISVpa2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISVisp3 is 95% (ORFA) and 94%% (ORFB) aa similar to ISVpa2. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Polz,M., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.