ISNeu4
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004757 | ND | Nitrosomonas europaea | Nitrosomonas europaea ATCC 19718 |
DNA section
IS Length : 1270 bp
Ends
IR Length : 26/34
IRL : TGACGTAGGTTGAAAAAAGTGGAGTCCAACGTTTCGTAAAATGTGAGGAA
IRR : TGACGTAGGTCGTCAACCGTAGAGTCCAACGGGTTAAGCGGCTATCTGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGACTTTCCT | GATTATGCGA | 0 |
DNA sequence
TGACGTAGGTTGAAAAAAGTGGAGTCCAACGTTTCGTAAAATGTGAGGAACGGAGGACAAGGATGGAACGACTCCCGAAAGGGATCTACACGCCGGAATT
TCGGGCGGAAGCGGTGAAGCTGGTGGAAGCGGAAGGGCTGTCGGTGGATGCGGCAGCGAAGCGCTTGTTGGTGCCAAAGAGCAGTCTTGGCAATTGGGTG
AGGGCATCGCGTACAGGCAGCCTGGCGAAGGTTGGGCAAGGTCAGCGGGTGCCGACGGAAACGGAAATTGAACTGGCGCGACTGCGCAAGGAATTGGCGG
AAGTGAAACTGGAGCGTGATCTGCTAAAAAAATGTGCGGCGTATTTCGCGAAGGAGTCGCGGTGAGATACGCCCGGATCGAACAGTTGCGACAGCACCAC
GCGGTGGCGGCGATGTGCCGGATTCTGGACGTTTCGGAAAGCGGCTATCACGCTTGGCGCCAACGTCCGCCGTCAGCTCGTCAGCAAGAGAACCTCCGGC
TGGAAACCGAGGTCAAGGCCGCGCATCAGCGGACGCGTGAAACCTACGGTCCCCGGCGCCTGCGGTCCGACCTGGCCGATCACGGCATCCAGACGAGCCT
CTATCGCATCAAGCGGATACGCAGGAAACTCGGGCTGCGCTGCAAGCAGAAGCGGAAATTCAAGGCGACGACAGATTCGAGGCATGCCCTGCCACTGGCG
CCCAATCTTCTCGATCGGCAATTCACCGTGGCGGCCCCCGATCGCGCCTGGGTCAGCGATATCACCTACGTCGCCACCGACGAGGGCTGGCTCTATCTTG
CCGGCATCAAGGATTTGTTCAACGGCGAACTGGTCGGCTACGCCATGTCCGAGCGGATGACCACGAGCCTGGTATCGCAAGCGCTCTTTCGCGCCGTCGC
CGCCAAGCGGCCAGCAAGGGGGCTGATCCATCATTCAGATCGTGGCAGCCAGTACTGCGCCCACGCTTATCGAAAGCAACTGCAACAATTCGGCATGCAA
GCTTCCATGAGCCGCAAGGGCAATTGTTGGGACAACGCGCCGATGGAGAGTTTCTGGGGTTCCCTGAAAAACGAGTTGGTGCATCACAGGCGCTTTACTA
CGCGCACACAAGCCCGGCAGGAAATCACCGAGTACATTGAAATCTTCTACAACCGAATTCGTAAGCAGGCGAGACTGGGCTATCTGTCGCCGGCACAATT
CACCCAGAAATACCATGCAAAACAGATAGCCGCTTAACCCGTTGGACTCTACGGTTGACGACCTACGTCA
TCGGGCGGAAGCGGTGAAGCTGGTGGAAGCGGAAGGGCTGTCGGTGGATGCGGCAGCGAAGCGCTTGTTGGTGCCAAAGAGCAGTCTTGGCAATTGGGTG
AGGGCATCGCGTACAGGCAGCCTGGCGAAGGTTGGGCAAGGTCAGCGGGTGCCGACGGAAACGGAAATTGAACTGGCGCGACTGCGCAAGGAATTGGCGG
AAGTGAAACTGGAGCGTGATCTGCTAAAAAAATGTGCGGCGTATTTCGCGAAGGAGTCGCGGTGAGATACGCCCGGATCGAACAGTTGCGACAGCACCAC
GCGGTGGCGGCGATGTGCCGGATTCTGGACGTTTCGGAAAGCGGCTATCACGCTTGGCGCCAACGTCCGCCGTCAGCTCGTCAGCAAGAGAACCTCCGGC
TGGAAACCGAGGTCAAGGCCGCGCATCAGCGGACGCGTGAAACCTACGGTCCCCGGCGCCTGCGGTCCGACCTGGCCGATCACGGCATCCAGACGAGCCT
CTATCGCATCAAGCGGATACGCAGGAAACTCGGGCTGCGCTGCAAGCAGAAGCGGAAATTCAAGGCGACGACAGATTCGAGGCATGCCCTGCCACTGGCG
CCCAATCTTCTCGATCGGCAATTCACCGTGGCGGCCCCCGATCGCGCCTGGGTCAGCGATATCACCTACGTCGCCACCGACGAGGGCTGGCTCTATCTTG
CCGGCATCAAGGATTTGTTCAACGGCGAACTGGTCGGCTACGCCATGTCCGAGCGGATGACCACGAGCCTGGTATCGCAAGCGCTCTTTCGCGCCGTCGC
CGCCAAGCGGCCAGCAAGGGGGCTGATCCATCATTCAGATCGTGGCAGCCAGTACTGCGCCCACGCTTATCGAAAGCAACTGCAACAATTCGGCATGCAA
GCTTCCATGAGCCGCAAGGGCAATTGTTGGGACAACGCGCCGATGGAGAGTTTCTGGGGTTCCCTGAAAAACGAGTTGGTGCATCACAGGCGCTTTACTA
CGCGCACACAAGCCCGGCAGGAAATCACCGAGTACATTGAAATCTTCTACAACCGAATTCGTAAGCAGGCGAGACTGGGCTATCTGTCGCCGGCACAATT
CACCCAGAAATACCATGCAAAACAGATAGCCGCTTAACCCGTTGGACTCTACGGTTGACGACCTACGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 303 bp | 100 aa | 63 | 365 | + | No |
Description : First part of the transposase
ORF sequence :
MERLPKGIYTPEFRAEAVKLVEAEGLSVDAAAKRLLVPKSSLGNWVRASRTGSLAKVGQGQRVPTETEIELARLRKELAEVKLERDLLKKCAAYFAKESR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 918 bp | 305 aa | 320 | 1237 | + | No |
Description : Second part of the transposase
ORF sequence :
SAKKMCGVFREGVAVRYARIEQLRQHHAVAAMCRILDVSESGYHAWRQRPPSARQQENLRLETEVKAAHQRTRETYGPRRLRSDLADHGIQTSLYRIKRI
RRKLGLRCKQKRKFKATTDSRHALPLAPNLLDRQFTVAAPDRAWVSDITYVATDEGWLYLAGIKDLFNGELVGYAMSERMTTSLVSQALFRAVAAKRPAR
GLIHHSDRGSQYCAHAYRKQLQQFGMQASMSRKGNCWDNAPMESFWGSLKNELVHHRRFTTRTQARQEITEYIEIFYNRIRKQARLGYLSPAQFTQKYHA
KQIAA
RRKLGLRCKQKRKFKATTDSRHALPLAPNLLDRQFTVAAPDRAWVSDITYVATDEGWLYLAGIKDLFNGELVGYAMSERMTTSLVSQALFRAVAAKRPAR
GLIHHSDRGSQYCAHAYRKQLQQFGMQASMSRKGNCWDNAPMESFWGSLKNELVHHRRFTTRTQARQEITEYIEIFYNRIRKQARLGYLSPAQFTQKYHA
KQIAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1175 bp | 391 aa | 63 | 1237 | + | Yes |
Chemistry : DDE
ORF sequence :
MERLPKGIYTPEFRAEAVKLVEAEGLSVDAAAKRLLVPKSSLGNWVRASRTGSLAKVGQGQRVPTETEIELARLRKELAEVKLERDLLKKMCGVFREGVA
VRYARIEQLRQHHAVAAMCRILDVSESGYHAWRQRPPSARQQENLRLETEVKAAHQRTRETYGPRRLRSDLADHGIQTSLYRIKRIRRKLGLRCKQKRKF
KATTDSRHALPLAPNLLDRQFTVAAPDRAWVSDITYVATDEGWLYLAGIKDLFNGELVGYAMSERMTTSLVSQALFRAVAAKRPARGLIHHSDRGSQYCA
HAYRKQLQQFGMQASMSRKGNCWDNAPMESFWGSLKNELVHHRRFTTRTQARQEITEYIEIFYNRIRKQARLGYLSPAQFTQKYHAKQIAA
VRYARIEQLRQHHAVAAMCRILDVSESGYHAWRQRPPSARQQENLRLETEVKAAHQRTRETYGPRRLRSDLADHGIQTSLYRIKRIRRKLGLRCKQKRKF
KATTDSRHALPLAPNLLDRQFTVAAPDRAWVSDITYVATDEGWLYLAGIKDLFNGELVGYAMSERMTTSLVSQALFRAVAAKRPARGLIHHSDRGSQYCA
HAYRKQLQQFGMQASMSRKGNCWDNAPMESFWGSLKNELVHHRRFTTRTQARQEITEYIEIFYNRIRKQARLGYLSPAQFTQKYHAKQIAA
Blast result :
Comments
ISNeu4 is 69% (ORFA) and 76% (ORFB) aa similar to ISGlo1 . The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chain,P., Lamerdin,J., Larimer,F., Regala,W., Lao,V., Land,M., Hauser,L., Hooper,A., Klotz,M., Norton,J., Sayavedra-Soto,L., Arciero,D., Hommes,N., Whittaker,M. and Arp,D. (2003) J. Bacteriol. 185 (9), 2759-2773.