ISRosp1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AANB01000001.1 | ND | Roseobacter sp. | Roseobacter sp. |
DNA section
IS Length : 1231 bp
Ends
IR Length : 23/28
IRL : TGGACCTGTCACGGTTTGATGCCGCTCCTTTGATAGCATTTACTGCAACA
IRR : TGGACCTGTCGCGTTTTAGTGCCGCCCCAAGTGCTCGTTTAAGCCACCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGTAGGCTC | ATTCGGACCT | 0 |
DNA sequence
TGGACCTGTCACGGTTTGATGCCGCTCCTTTGATAGCATTTACTGCAACAAAGGAGATGAACTATGGGACTGAAACGGACAGACGAATTTCGACAAGATG
CGGTGCGCATCGCATTAACCAGTGGGCTGACGCGTAAGCAGGTGGCTGACGATCTGGGTGTTGGCATGTCGACGTTGAACAAATGGATCACTGCACACCG
AGACACAGACGTGGTTTCCAAAGAGGATTTGAGCCTGGCTCAAGAGAATGATCGACTTCGACGGGAGAACCGGCTCCTGAAGGAGGAGAGGGAGATCTTA
AAAAAAGCCACCCAGTTCTTTGCGGGCCTAAAGCAATGAGATTTAGGTTTGTCGAAGAACACAGGGGCAGCTTTCCAGCCGCCCGGTTGTGCGAGGTCGT
CGGTGTCAGCGCACGCGGGTTACGCGCATTCCGCAGTCGCCCAGCCAGTCGTAGGCAGCGATCTGATCTGGTCACGCTGGCTCATATCAAAGAGCAGTCG
CGGCTTAGTTTGGGGAGCTATGGCCGCCCACGAATGACCGAAGAGTTGAAGGAGATCGGTTTGAACGTGGGCCACCGTCGTGTTGGCAGGTTGATGCGCC
AGAACGGGATATCTGTTGTCAGAACACGCAAACACAAGGTCACGACAGACAGTGACCACAAGTTCAATATCGCGCCGAACTTGCTGGATCGAAACTTCTC
AGCTGACCTGCCAAATCAAAAGTGGGCGGGGGATATCAGCTATGTCTGGACCCGCGAAGGCTGGCTGTATCTGGCCGTGATCCTCGATCTGCATTCAAGG
CGTGTTATCGGCTGGGCCGTGAGCAATCGGATGAAACGCGACTTGGCGATCCGGGCGTTGAATATGGCCATCGCGTTCCGGCAGCCGCCCAAAGGCTGCA
TCCATCATACGGATCGCGGGTCGCAATATTGTTCCCATGACTACCAGAAAATCCTGCGCCAGAACGGCTTCAAAGTATCGATGAGCGGTAAGGGTAATTG
TTACGACAACGCCGCCGTCGAGACGTTCTTCAAGACCATCAAAGCTGAACTGATCTGGCGGCATCCTTGGGAGACCCGTCGGAAGGCTGAAATGGCGATC
TTCGAATACATAAACGGGTTCTACAATCCACGCCGCCGTCACTCAGCACTGGGCTGGAAAAGCCCTGTCGCCTTCGAACGAAAGGTGGCTTAAACGAGCA
CTTGGGGCGGCACTAAAACGCGACAGGTCCA
CGGTGCGCATCGCATTAACCAGTGGGCTGACGCGTAAGCAGGTGGCTGACGATCTGGGTGTTGGCATGTCGACGTTGAACAAATGGATCACTGCACACCG
AGACACAGACGTGGTTTCCAAAGAGGATTTGAGCCTGGCTCAAGAGAATGATCGACTTCGACGGGAGAACCGGCTCCTGAAGGAGGAGAGGGAGATCTTA
AAAAAAGCCACCCAGTTCTTTGCGGGCCTAAAGCAATGAGATTTAGGTTTGTCGAAGAACACAGGGGCAGCTTTCCAGCCGCCCGGTTGTGCGAGGTCGT
CGGTGTCAGCGCACGCGGGTTACGCGCATTCCGCAGTCGCCCAGCCAGTCGTAGGCAGCGATCTGATCTGGTCACGCTGGCTCATATCAAAGAGCAGTCG
CGGCTTAGTTTGGGGAGCTATGGCCGCCCACGAATGACCGAAGAGTTGAAGGAGATCGGTTTGAACGTGGGCCACCGTCGTGTTGGCAGGTTGATGCGCC
AGAACGGGATATCTGTTGTCAGAACACGCAAACACAAGGTCACGACAGACAGTGACCACAAGTTCAATATCGCGCCGAACTTGCTGGATCGAAACTTCTC
AGCTGACCTGCCAAATCAAAAGTGGGCGGGGGATATCAGCTATGTCTGGACCCGCGAAGGCTGGCTGTATCTGGCCGTGATCCTCGATCTGCATTCAAGG
CGTGTTATCGGCTGGGCCGTGAGCAATCGGATGAAACGCGACTTGGCGATCCGGGCGTTGAATATGGCCATCGCGTTCCGGCAGCCGCCCAAAGGCTGCA
TCCATCATACGGATCGCGGGTCGCAATATTGTTCCCATGACTACCAGAAAATCCTGCGCCAGAACGGCTTCAAAGTATCGATGAGCGGTAAGGGTAATTG
TTACGACAACGCCGCCGTCGAGACGTTCTTCAAGACCATCAAAGCTGAACTGATCTGGCGGCATCCTTGGGAGACCCGTCGGAAGGCTGAAATGGCGATC
TTCGAATACATAAACGGGTTCTACAATCCACGCCGCCGTCACTCAGCACTGGGCTGGAAAAGCCCTGTCGCCTTCGAACGAAAGGTGGCTTAAACGAGCA
CTTGGGGCGGCACTAAAACGCGACAGGTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 64 | 339 | + | No |
Description : First part of the transposase
ORF sequence :
MGLKRTDEFRQDAVRIALTSGLTRKQVADDLGVGMSTLNKWITAHRDTDVVSKEDLSLAQENDRLRRENRLLKEEREILKKATQFFAGLKQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 942 bp | 313 aa | 252 | 1193 | + | No |
Description : Second part of the transposase
ORF sequence :
STSTGEPAPEGGEGDLKKSHPVLCGPKAMRFRFVEEHRGSFPAARLCEVVGVSARGLRAFRSRPASRRQRSDLVTLAHIKEQSRLSLGSYGRPRMTEELK
EIGLNVGHRRVGRLMRQNGISVVRTRKHKVTTDSDHKFNIAPNLLDRNFSADLPNQKWAGDISYVWTREGWLYLAVILDLHSRRVIGWAVSNRMKRDLAI
RALNMAIAFRQPPKGCIHHTDRGSQYCSHDYQKILRQNGFKVSMSGKGNCYDNAAVETFFKTIKAELIWRHPWETRRKAEMAIFEYINGFYNPRRRHSAL
GWKSPVAFERKVA
EIGLNVGHRRVGRLMRQNGISVVRTRKHKVTTDSDHKFNIAPNLLDRNFSADLPNQKWAGDISYVWTREGWLYLAVILDLHSRRVIGWAVSNRMKRDLAI
RALNMAIAFRQPPKGCIHHTDRGSQYCSHDYQKILRQNGFKVSMSGKGNCYDNAAVETFFKTIKAELIWRHPWETRRKAEMAIFEYINGFYNPRRRHSAL
GWKSPVAFERKVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1130 bp | 376 aa | 64 | 1193 | + | Yes |
Chemistry : DDE
ORF sequence :
MGLKRTDEFRQDAVRIALTSGLTRKQVADDLGVGMSTLNKWITAHRDTDVVSKEDLSLAQENDRLRRENRLLKEEREILKKSHPVLCGPKAMRFRFVEEH
RGSFPAARLCEVVGVSARGLRAFRSRPASRRQRSDLVTLAHIKEQSRLSLGSYGRPRMTEELKEIGLNVGHRRVGRLMRQNGISVVRTRKHKVTTDSDHK
FNIAPNLLDRNFSADLPNQKWAGDISYVWTREGWLYLAVILDLHSRRVIGWAVSNRMKRDLAIRALNMAIAFRQPPKGCIHHTDRGSQYCSHDYQKILRQ
NGFKVSMSGKGNCYDNAAVETFFKTIKAELIWRHPWETRRKAEMAIFEYINGFYNPRRRHSALGWKSPVAFERKVA
RGSFPAARLCEVVGVSARGLRAFRSRPASRRQRSDLVTLAHIKEQSRLSLGSYGRPRMTEELKEIGLNVGHRRVGRLMRQNGISVVRTRKHKVTTDSDHK
FNIAPNLLDRNFSADLPNQKWAGDISYVWTREGWLYLAVILDLHSRRVIGWAVSNRMKRDLAIRALNMAIAFRQPPKGCIHHTDRGSQYCSHDYQKILRQ
NGFKVSMSGKGNCYDNAAVETFFKTIKAELIWRHPWETRRKAEMAIFEYINGFYNPRRRHSALGWKSPVAFERKVA
Blast result :
Comments
Genome in progress.
ISRosp1 is 79% (ORFA) and 85% (ORFB) aa similar to IS1133. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
ISRosp1 is 79% (ORFA) and 85% (ORFB) aa similar to IS1133. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Pinhassi,J., Pedros-Alio,C., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.