ISPssp1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAPX01000001 | ND | Psychrobacter sp. | Psychrobacter sp. |
DNA section
IS Length : 1276 bp
Ends
IR Length : 35/48
IRL : TGATGTATGCTGAAAAAAACGGACAAAAATATTTGGTAAACTAGAATCCT
IRR : TGACCTCTGCTGTCAACAACGGAGAAAAACATTTGGTAGATTTCATCAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAAGCTTTAAG | GTAAGGTATA | 0 |
DNA sequence
TGATGTATGCTGAAAAAAACGGACAAAAATATTTGGTAAACTAGAATCCTAAACAGGAGTTTACCATGAGTAAGAAAAGAGTAGAATACAGCGACACTTT
CAAAGCTGAAGCCATCGCCAAAGTCAAAGAAAATAACGGCAACATCAGTCAAACGGCTAAAGAACTTGGCATACCCATGAACACACTAGCAAACTGGCAT
AGGAAGGCTGAAAGAGGCGTACTTGCAGGCACCCAAAATTACAATTCTGAACTCATAGCCGCCTTGGATGAAATCAAAGACCTAAAAAAACAACTAAGAA
TCGCTCAAGAGGAACGAGAAATTCTAAAAAAGGCAACGGCGTACTTTGCCAAGAATCAATAGCCAAGTACGCCTTTATGCAAGAGAATCGATTGAAATAC
TGCATACAACGTATGGCGACCGTATTTGAAGTCAGCCTATCAGGCTACTATGACTGGCTAAAGCGAGGCATGAGCAAGCGAAAACAGCACCACAATCGCT
GTGAGTTACTGGTCAAATCCGCTCATATGGATACACAGCAAAGCTATGGGCATGAACGCTTGCATCAGCATTTAACCAGCCAAGGACACGACATCAGCCT
GTACATGGTTCGGCAAATCAAGCAAGAACATGGTATTTACTGCAAACGCCACAAGCGATCTAAGGTGACTACTGACTCAAATCATAATAAGCCTGTGTAT
CCAAACTTGTTAGAACAGCAGTTTGACGTGGCAGCACCCAATATCGCTTGGGTTAGCGATATTACCTATATTTGGACCAATGAAGGCTGGGTGTACTTGG
CAGCGTTCAAAGACTTGTACAGCAAAGAGATTGTGGGATATGCTCTAAATAAGCGTATGACCGCTGATTTGGTTTGTGAGGCTTTAAACAATGCCATTAA
GTACAAACGCCCTGCTCGGGGCTTAATCGTGCATTCTGACAGGGGTAGTCAGTATTGTAGCCATCAATACCGCCAAATCATCGACAAGTATGGTTTTGCT
GGCTCTATGAGCCGAAAAGGCAATTGCTACGATAACGCCCCGATTGAAAGCTTCTGGGGTCAGTTAAAAAACGAGCTGATTTATCACAAAGTCTATGAGA
CTCGTGATGAAGCCATTAAGGATGTGGTTAGATATATTGAGATTTTTTACAACCGTCAACGAATACAAAAAGGGTTAGGATTTAAGTCACCTACTCAAGT
ATTCCAAGACTTTTACCGTCAGGCTGCCTGATGAAATCTACCAAATGTTTTTCTCCGTTGTTGACAGCAGAGGTCA
CAAAGCTGAAGCCATCGCCAAAGTCAAAGAAAATAACGGCAACATCAGTCAAACGGCTAAAGAACTTGGCATACCCATGAACACACTAGCAAACTGGCAT
AGGAAGGCTGAAAGAGGCGTACTTGCAGGCACCCAAAATTACAATTCTGAACTCATAGCCGCCTTGGATGAAATCAAAGACCTAAAAAAACAACTAAGAA
TCGCTCAAGAGGAACGAGAAATTCTAAAAAAGGCAACGGCGTACTTTGCCAAGAATCAATAGCCAAGTACGCCTTTATGCAAGAGAATCGATTGAAATAC
TGCATACAACGTATGGCGACCGTATTTGAAGTCAGCCTATCAGGCTACTATGACTGGCTAAAGCGAGGCATGAGCAAGCGAAAACAGCACCACAATCGCT
GTGAGTTACTGGTCAAATCCGCTCATATGGATACACAGCAAAGCTATGGGCATGAACGCTTGCATCAGCATTTAACCAGCCAAGGACACGACATCAGCCT
GTACATGGTTCGGCAAATCAAGCAAGAACATGGTATTTACTGCAAACGCCACAAGCGATCTAAGGTGACTACTGACTCAAATCATAATAAGCCTGTGTAT
CCAAACTTGTTAGAACAGCAGTTTGACGTGGCAGCACCCAATATCGCTTGGGTTAGCGATATTACCTATATTTGGACCAATGAAGGCTGGGTGTACTTGG
CAGCGTTCAAAGACTTGTACAGCAAAGAGATTGTGGGATATGCTCTAAATAAGCGTATGACCGCTGATTTGGTTTGTGAGGCTTTAAACAATGCCATTAA
GTACAAACGCCCTGCTCGGGGCTTAATCGTGCATTCTGACAGGGGTAGTCAGTATTGTAGCCATCAATACCGCCAAATCATCGACAAGTATGGTTTTGCT
GGCTCTATGAGCCGAAAAGGCAATTGCTACGATAACGCCCCGATTGAAAGCTTCTGGGGTCAGTTAAAAAACGAGCTGATTTATCACAAAGTCTATGAGA
CTCGTGATGAAGCCATTAAGGATGTGGTTAGATATATTGAGATTTTTTACAACCGTCAACGAATACAAAAAGGGTTAGGATTTAAGTCACCTACTCAAGT
ATTCCAAGACTTTTACCGTCAGGCTGCCTGATGAAATCTACCAAATGTTTTTCTCCGTTGTTGACAGCAGAGGTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 66 | 362 | + | No |
Description : First part of the transposase
ORF sequence :
MSKKRVEYSDTFKAEAIAKVKENNGNISQTAKELGIPMNTLANWHRKAERGVLAGTQNYNSELIAALDEIKDLKKQLRIAQEEREILKKATAYFAKNQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 960 bp | 319 aa | 272 | 1231 | + | No |
Description : Second part of the transposase
ORF sequence :
NQRPKKTTKNRSRGTRNSKKGNGVLCQESIAKYAFMQENRLKYCIQRMATVFEVSLSGYYDWLKRGMSKRKQHHNRCELLVKSAHMDTQQSYGHERLHQH
LTSQGHDISLYMVRQIKQEHGIYCKRHKRSKVTTDSNHNKPVYPNLLEQQFDVAAPNIAWVSDITYIWTNEGWVYLAAFKDLYSKEIVGYALNKRMTADL
VCEALNNAIKYKRPARGLIVHSDRGSQYCSHQYRQIIDKYGFAGSMSRKGNCYDNAPIESFWGQLKNELIYHKVYETRDEAIKDVVRYIEIFYNRQRIQK
GLGFKSPTQVFQDFYRQAA
LTSQGHDISLYMVRQIKQEHGIYCKRHKRSKVTTDSNHNKPVYPNLLEQQFDVAAPNIAWVSDITYIWTNEGWVYLAAFKDLYSKEIVGYALNKRMTADL
VCEALNNAIKYKRPARGLIVHSDRGSQYCSHQYRQIIDKYGFAGSMSRKGNCYDNAPIESFWGQLKNELIYHKVYETRDEAIKDVVRYIEIFYNRQRIQK
GLGFKSPTQVFQDFYRQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 66 | 1231 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKKRVEYSDTFKAEAIAKVKENNGNISQTAKELGIPMNTLANWHRKAERGVLAGTQNYNSELIAALDEIKDLKKQLRIAQEEREILKKGNGVLCQESIA
KYAFMQENRLKYCIQRMATVFEVSLSGYYDWLKRGMSKRKQHHNRCELLVKSAHMDTQQSYGHERLHQHLTSQGHDISLYMVRQIKQEHGIYCKRHKRSK
VTTDSNHNKPVYPNLLEQQFDVAAPNIAWVSDITYIWTNEGWVYLAAFKDLYSKEIVGYALNKRMTADLVCEALNNAIKYKRPARGLIVHSDRGSQYCSH
QYRQIIDKYGFAGSMSRKGNCYDNAPIESFWGQLKNELIYHKVYETRDEAIKDVVRYIEIFYNRQRIQKGLGFKSPTQVFQDFYRQAA
KYAFMQENRLKYCIQRMATVFEVSLSGYYDWLKRGMSKRKQHHNRCELLVKSAHMDTQQSYGHERLHQHLTSQGHDISLYMVRQIKQEHGIYCKRHKRSK
VTTDSNHNKPVYPNLLEQQFDVAAPNIAWVSDITYIWTNEGWVYLAAFKDLYSKEIVGYALNKRMTADLVCEALNNAIKYKRPARGLIVHSDRGSQYCSH
QYRQIIDKYGFAGSMSRKGNCYDNAPIESFWGQLKNELIYHKVYETRDEAIKDVVRYIEIFYNRQRIQKGLGFKSPTQVFQDFYRQAA
Blast result :
Comments
Genome in progress.
ISPssp1 is 59 % (ORFA) aa similar to ISDha6 and 69% (ORFB) aa similar to ISGlo1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPssp1 is 59 % (ORFA) aa similar to ISDha6 and 69% (ORFB) aa similar to ISGlo1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Direct submission GenBank.