ISGpr2
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAOA01000003.1 | ND | Gamma proteobacterium | Gamma proteobacterium KT 71 |
DNA section
IS Length : 1229 bp
Ends
IR Length : 34/34
IRL : TGAATCGCCACCAGTTTGCTAGACACCTTTCAGCCATACAAAATGGCTAT
IRR : TGAATCGCCACCAGTTTGCTAGACACCTTTCAGCTGTTTTTCGTGATGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGGAGCTTT | GGC | AGGCCGCCGC | 3 |
DNA sequence
TGAATCGCCACCAGTTTGCTAGACACCTTTCAGCCATACAAAATGGCTATCAGAGAGGTGTTTATGAGCAACAAGCGTTACCCCGAAGAATTCAAGAGAG
AAGCCGTTCGCCAGATCACGGATCGCGGCTACAGCGTGGCGGACGTGGCCAGTCGGCTGGGCGTGACGACGCACAGTCTGTATGCCTGGGTGAAGAAGTA
CGGGCCGGCTGCTGAGCAACATCGAGTGGAGGCTGATGATCAGGCCGAGATTCGTCGGCTACAAAAAGAGCTTAAGCGTGTCACGGAGGAGCGTGACATT
CTAAAAAAAGCCGCGGCGTACTTCGCGAGCCAATCCGAGTGAGGTACGCTTTTATTCAATCCCATCGCCACCAGTTGCCGGTGCGGCATCTGTGTTCTTT
GCTTAACGTTCACCCCAGCGGTTTCTATGCGTGGCGTCGACAGCCGCTATCGGCTCGCGGTTGTGAGGACAAGCGGCTCTCTGGCCTGATCAAGCAGTTC
TGGCTCGAGTCCGGTGCTGTGTATGGCTATCGGAAGATCCATCGTGATTTGCATGAAATAGGCGAGTGTTGCGGCCCCAACCGTGTCCACCGTCTCATGA
AAGACGCTGGAATCAGGGCGCAGGTTGGATATCGCAAGCCACGGCATAAGGTTGGCAAAGCGCATGATGTGACGCCTAACGTACTGCAGAGACAGTTTAA
TCCGGCTGCGCCGAACGAGCGATGGGCGACCGACATCACCCATATTCGGACCCACGAAGGCTGGTTGTACCTGGCCGTGGTCATCGACTTATTCTCGCGC
CGGGTGATTGGCTGGTCGATGCAATCACGGATCACGAGCGATCTGGTTCTAGATGCCTTGTTGATGTCGGTGTGGCGTCGCAAGCCGGTGAGCAAGGTAC
TTGTTCATTCGGACCAGGGCAGTCAATACACAAGCTATGACTGGAGCGCGTTTCTACGCGCAAACGGCCTTGAGGGCAGTATGAGCAGACGCGGCAACTG
CCATGACAACGCAGTCGCTGAAAGCTTCTTCCAGCTTCTGAAACGCGAGCGCGTGAAGCGCAAGATATATCCGACCCGGCAGGATGCCCGGGCCGATATC
TTTGACTATATCGAGATGTTCTACAACGTCAGACGCCGGCATGGCTCGAACAGCCTGCTGTCACCTGTAGACTACGAACAGCATCACGAAAAACAGCTGA
AAGGTGTCTAGCAAACTGGTGGCGATTCA
AAGCCGTTCGCCAGATCACGGATCGCGGCTACAGCGTGGCGGACGTGGCCAGTCGGCTGGGCGTGACGACGCACAGTCTGTATGCCTGGGTGAAGAAGTA
CGGGCCGGCTGCTGAGCAACATCGAGTGGAGGCTGATGATCAGGCCGAGATTCGTCGGCTACAAAAAGAGCTTAAGCGTGTCACGGAGGAGCGTGACATT
CTAAAAAAAGCCGCGGCGTACTTCGCGAGCCAATCCGAGTGAGGTACGCTTTTATTCAATCCCATCGCCACCAGTTGCCGGTGCGGCATCTGTGTTCTTT
GCTTAACGTTCACCCCAGCGGTTTCTATGCGTGGCGTCGACAGCCGCTATCGGCTCGCGGTTGTGAGGACAAGCGGCTCTCTGGCCTGATCAAGCAGTTC
TGGCTCGAGTCCGGTGCTGTGTATGGCTATCGGAAGATCCATCGTGATTTGCATGAAATAGGCGAGTGTTGCGGCCCCAACCGTGTCCACCGTCTCATGA
AAGACGCTGGAATCAGGGCGCAGGTTGGATATCGCAAGCCACGGCATAAGGTTGGCAAAGCGCATGATGTGACGCCTAACGTACTGCAGAGACAGTTTAA
TCCGGCTGCGCCGAACGAGCGATGGGCGACCGACATCACCCATATTCGGACCCACGAAGGCTGGTTGTACCTGGCCGTGGTCATCGACTTATTCTCGCGC
CGGGTGATTGGCTGGTCGATGCAATCACGGATCACGAGCGATCTGGTTCTAGATGCCTTGTTGATGTCGGTGTGGCGTCGCAAGCCGGTGAGCAAGGTAC
TTGTTCATTCGGACCAGGGCAGTCAATACACAAGCTATGACTGGAGCGCGTTTCTACGCGCAAACGGCCTTGAGGGCAGTATGAGCAGACGCGGCAACTG
CCATGACAACGCAGTCGCTGAAAGCTTCTTCCAGCTTCTGAAACGCGAGCGCGTGAAGCGCAAGATATATCCGACCCGGCAGGATGCCCGGGCCGATATC
TTTGACTATATCGAGATGTTCTACAACGTCAGACGCCGGCATGGCTCGAACAGCCTGCTGTCACCTGTAGACTACGAACAGCATCACGAAAAACAGCTGA
AAGGTGTCTAGCAAACTGGTGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 300 bp | 99 aa | 43 | 342 | + | No |
Description : First part of the transposase
ORF sequence :
MAIREVFMSNKRYPEEFKREAVRQITDRGYSVADVASRLGVTTHSLYAWVKKYGPAAEQHRVEADDQAEIRRLQKELKRVTEERDILKKAAAYFASQSE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 915 bp | 304 aa | 297 | 1211 | + | No |
Description : Second part of the transposase
ORF sequence :
HSKKSRGVLREPIRVRYAFIQSHRHQLPVRHLCSLLNVHPSGFYAWRRQPLSARGCEDKRLSGLIKQFWLESGAVYGYRKIHRDLHEIGECCGPNRVHRL
MKDAGIRAQVGYRKPRHKVGKAHDVTPNVLQRQFNPAAPNERWATDITHIRTHEGWLYLAVVIDLFSRRVIGWSMQSRITSDLVLDALLMSVWRRKPVSK
VLVHSDQGSQYTSYDWSAFLRANGLEGSMSRRGNCHDNAVAESFFQLLKRERVKRKIYPTRQDARADIFDYIEMFYNVRRRHGSNSLLSPVDYEQHHEKQ
LKGV
MKDAGIRAQVGYRKPRHKVGKAHDVTPNVLQRQFNPAAPNERWATDITHIRTHEGWLYLAVVIDLFSRRVIGWSMQSRITSDLVLDALLMSVWRRKPVSK
VLVHSDQGSQYTSYDWSAFLRANGLEGSMSRRGNCHDNAVAESFFQLLKRERVKRKIYPTRQDARADIFDYIEMFYNVRRRHGSNSLLSPVDYEQHHEKQ
LKGV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1169 bp | 389 aa | 43 | 1211 | + | Yes |
Chemistry : DDE
ORF sequence :
MAIREVFMSNKRYPEEFKREAVRQITDRGYSVADVASRLGVTTHSLYAWVKKYGPAAEQHRVEADDQAEIRRLQKELKRVTEERDILKKSRGVLREPIRV
RYAFIQSHRHQLPVRHLCSLLNVHPSGFYAWRRQPLSARGCEDKRLSGLIKQFWLESGAVYGYRKIHRDLHEIGECCGPNRVHRLMKDAGIRAQVGYRKP
RHKVGKAHDVTPNVLQRQFNPAAPNERWATDITHIRTHEGWLYLAVVIDLFSRRVIGWSMQSRITSDLVLDALLMSVWRRKPVSKVLVHSDQGSQYTSYD
WSAFLRANGLEGSMSRRGNCHDNAVAESFFQLLKRERVKRKIYPTRQDARADIFDYIEMFYNVRRRHGSNSLLSPVDYEQHHEKQLKGV
RYAFIQSHRHQLPVRHLCSLLNVHPSGFYAWRRQPLSARGCEDKRLSGLIKQFWLESGAVYGYRKIHRDLHEIGECCGPNRVHRLMKDAGIRAQVGYRKP
RHKVGKAHDVTPNVLQRQFNPAAPNERWATDITHIRTHEGWLYLAVVIDLFSRRVIGWSMQSRITSDLVLDALLMSVWRRKPVSKVLVHSDQGSQYTSYD
WSAFLRANGLEGSMSRRGNCHDNAVAESFFQLLKRERVKRKIYPTRQDARADIFDYIEMFYNVRRRHGSNSLLSPVDYEQHHEKQLKGV
Blast result :
Comments
Genome in progress.
ISGpr2 is 84 % (ORFA) aa similar to ISEhe3 and 80% (ORFB) aa similar to ISPca2.
The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
ISGpr2 is 84 % (ORFA) aa similar to ISEhe3 and 80% (ORFB) aa similar to ISPca2.
The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Amann,R., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.