ISLin2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004342 | ND | Leptospira interrogans | Leptospira interrogans serovar Lai |
DNA section
IS Length : 1236 bp
Ends
IR Length : 30/39
IRL : TAACCTAGTTACGTAAAAACGGACACCTTTTACTCAGCGAATGAGGTAAA
IRR : TAACCTAGTGACGAATTATTGGACACATTTTTACGCAGCATTTACTTCAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTAATTGAA | TAC | TCTCTTAAAC | 3 |
DNA sequence
TAACCTAGTTACGTAAAAACGGACACCTTTTACTCAGCGAATGAGGTAAAGGTGAATATGAATAAGAAAGGAAAATACTCTCCGGAGTTTAAAGAACAAG
CAGTCAAGCGAACACTTTCTGGTTCGTTTACGATAAAAGAAGTAGCAGGGTCACTTGGAATCAGCTATTTTGTGTTACGACAATGGAGGGGAGAATACTT
GAAAAAATCAGAAGATCAGCTTCCACTTACGGATAAACAGTTAAAAGAAAGTGAAGAACTGAAAAAGCTTCGGAAGGAAAATCTGAAACTGAAAGAGGAA
GTTACTATCCTAAAAAAGTTTGCCGCCATGCTTTCCCGCGAACAAAATCCCGATTGATGTTCATGGAAACTCATCGATTTGAGTATTCGATTCAAAGCAT
GGCTAACGTTTTAGGGGTGTCTCGTTCAGGATTCTATCAATTTCTAAAACGGAGTAAAAACGAATTAGAAAAATATAATCCTGAACTTGTCGAGTTCATT
CGGGAAACGTGGCTAACAAGCCGTAAGAATTACGGCTTAGTTCGGTTGCTTCGGGAAGTGAAGAAAGTGTATTCAATTTACGGAGCAAGAACGGTTCGAA
AAGTGATGAAACTTTGTGAAATTCAAGGAAAACAGGAGAAACGTTTTCGGATTGCCACAACAGATTCTAATCATGGGAATCGGGTTGCTCCGAACTTAGT
TCAACGGAATTTCAAACCGAATCAGAAGAATCGGATCTGGGGTTTCAGATATTACTTTTTTAAGATCCTCTTTCGGGTGGATTTATCTTTGTGTAATACT
GGATCTTTATTCTCGAAAAGTAGTGGGTTGGTCGATTTCAAATTCTAATGATTCTAAGTTAGTGTGCACCGCTCTTTCCAAAGCAATCGAATGTAGAAAT
CCTCCTAAGGGTTTAGTTTTTCATTCGGATCGAGGATCCAATTATTGTTCGTACGAAACTCGAAGGTATCTGTTAAATAATAAACTCAGAAGGAGTAATA
GTAGAAAGGGAAATTGTTGGGACAACGCAGTTGCTGAGTCCTTCTTTGGTTCTCTGAAAAGAGAAATGGAATATAATTACTTTTATAAAATTCAAGAAGC
CGAAGAATTACTTTTTGATCATATAGAGGTTTATTATAATAGACACAGATCTCATTCGTCATTAGACTTTGTGAGTCCTGTGCAATTTGAAGTAAATGCT
GCGTAAAAATGTGTCCAATAATTCGTCACTAGGTTA
CAGTCAAGCGAACACTTTCTGGTTCGTTTACGATAAAAGAAGTAGCAGGGTCACTTGGAATCAGCTATTTTGTGTTACGACAATGGAGGGGAGAATACTT
GAAAAAATCAGAAGATCAGCTTCCACTTACGGATAAACAGTTAAAAGAAAGTGAAGAACTGAAAAAGCTTCGGAAGGAAAATCTGAAACTGAAAGAGGAA
GTTACTATCCTAAAAAAGTTTGCCGCCATGCTTTCCCGCGAACAAAATCCCGATTGATGTTCATGGAAACTCATCGATTTGAGTATTCGATTCAAAGCAT
GGCTAACGTTTTAGGGGTGTCTCGTTCAGGATTCTATCAATTTCTAAAACGGAGTAAAAACGAATTAGAAAAATATAATCCTGAACTTGTCGAGTTCATT
CGGGAAACGTGGCTAACAAGCCGTAAGAATTACGGCTTAGTTCGGTTGCTTCGGGAAGTGAAGAAAGTGTATTCAATTTACGGAGCAAGAACGGTTCGAA
AAGTGATGAAACTTTGTGAAATTCAAGGAAAACAGGAGAAACGTTTTCGGATTGCCACAACAGATTCTAATCATGGGAATCGGGTTGCTCCGAACTTAGT
TCAACGGAATTTCAAACCGAATCAGAAGAATCGGATCTGGGGTTTCAGATATTACTTTTTTAAGATCCTCTTTCGGGTGGATTTATCTTTGTGTAATACT
GGATCTTTATTCTCGAAAAGTAGTGGGTTGGTCGATTTCAAATTCTAATGATTCTAAGTTAGTGTGCACCGCTCTTTCCAAAGCAATCGAATGTAGAAAT
CCTCCTAAGGGTTTAGTTTTTCATTCGGATCGAGGATCCAATTATTGTTCGTACGAAACTCGAAGGTATCTGTTAAATAATAAACTCAGAAGGAGTAATA
GTAGAAAGGGAAATTGTTGGGACAACGCAGTTGCTGAGTCCTTCTTTGGTTCTCTGAAAAGAGAAATGGAATATAATTACTTTTATAAAATTCAAGAAGC
CGAAGAATTACTTTTTGATCATATAGAGGTTTATTATAATAGACACAGATCTCATTCGTCATTAGACTTTGTGAGTCCTGTGCAATTTGAAGTAAATGCT
GCGTAAAAATGTGTCCAATAATTCGTCACTAGGTTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 99 aa | 61 | 357 | + | No |
Description : First part of the transposase
ORF sequence :
MNKKGKYSPEFKEQAVKRTLSGSFTIKEVAGSLGISYFVLRQWRGEYLKKSEDQLPLTDKQLKESEELKKLRKENLKLKEEVTILKKFAAMLSREQNPD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 594 bp | 197 aa | 255 | 848 | + | No |
Description : Second part of the transposase
ORF sequence :
RTEKASEGKSETERGSYYPKKVCRHAFPRTKSRLMFMETHRFEYSIQSMANVLGVSRSGFYQFLKRSKNELEKYNPELVEFIRETWLTSRKNYGLVRLLR
EVKKVYSIYGARTVRKVMKLCEIQGKQEKRFRIATTDSNHGNRVAPNLVQRNFKPNQKNRIWGFRYYFFKILFRVDLSLCNTGSLFSKSSGLVDFKF
EVKKVYSIYGARTVRKVMKLCEIQGKQEKRFRIATTDSNHGNRVAPNLVQRNFKPNQKNRIWGFRYYFFKILFRVDLSLCNTGSLFSKSSGLVDFKF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 788 bp | 263 aa | 61 | 848 | + | Yes |
Chemistry : DDE
ORF sequence :
MNKKGKYSPEFKEQAVKRTLSGSFTIKEVAGSLGISYFVLRQWRGEYLKKSEDQLPLTDKQLKESEELKKLRKENLKLKEEVTILKKVCRHAFPRTKSRL
MFMETHRFEYSIQSMANVLGVSRSGFYQFLKRSKNELEKYNPELVEFIRETWLTSRKNYGLVRLLREVKKVYSIYGARTVRKVMKLCEIQGKQEKRFRIA
TTDSNHGNRVAPNLVQRNFKPNQKNRIWGFRYYFFKILFRVDLSLCNTGSLFSKSSGLVDFKF
MFMETHRFEYSIQSMANVLGVSRSGFYQFLKRSKNELEKYNPELVEFIRETWLTSRKNYGLVRLLREVKKVYSIYGARTVRKVMKLCEIQGKQEKRFRIA
TTDSNHGNRVAPNLVQRNFKPNQKNRIWGFRYYFFKILFRVDLSLCNTGSLFSKSSGLVDFKF
Blast result :
Comments
ISLin2 is 100% (ORFA) and 90% (ORFB) aa similar to IS1500B. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Ren,S.X., Fu,G., Jiang,X.G., Zeng,R., Miao,Y.G., Xu,H., Zhang,Y.X., Xiong,H., Lu,G., Lu,L.F., Jiang,H.Q., Jia,J., Tu,Y.F., Jiang,J.X., Gu,W.Y., Zhang,Y.Q., Cai,Z., Sheng,H.H., Yin,H.F., Zhang,Y., Zhu,G.F., Wan,M., Huang,H.L., Qian,Z., Wang,S.Y., Ma,W., Yao,Z.J., Shen,Y., Qiang,B.Q., Xia,Q.C., Guo,X.K., Danchin,A., Saint Girons,I., Somerville,R.L., Wen,Y.M., Shi,M.H., Chen,Z., Xu,J.G. and Zhao,G.P. (2003) Nature 422 (6934), 888-893