ISNieu3
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008344 | ND | Nitrosomonas eutropha | Nitrosomonas eutropha C91 |
DNA section
IS Length : 1266 bp
Ends
IR Length : 25/43
IRL : TGATATGGTCTAATAGACCCGGACACTCCAGTTAAGAGAAAATAGTCTTA
IRR : TAACGTGGTCAAGTAAAATCGGACACCTCAGTTAAGCTGCTTTTATCGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAATTGATA | CGTTAGTTTG | 0 |
DNA sequence
TGATATGGTCTAATAGACCCGGACACTCCAGTTAAGAGAAAATAGTCTTAATTGGAGGATGAAATGGAGCCTGGGAAACACTATACAAAGGAATTCAAGC
TGGATGCAATCAGCTTGGTACTGGATCAGGGATTAACGATAGCAGAAGCTGCCAGAAGCCTGGAAATCAGGGCTAACATGCTTGGGCGATGGATCAGGGA
GAGCCAGGCGGATACTAATGGTCAGGCATTCCGCGGTAATGGGAAACTGACACCGGAGCAGGAAGAAATCCGGCGGCTTAAACTAGAAAACAAGCAATTA
AAGCTTGAGCGGCAAATACTAAAGGAGGCGGCGGTCTTTTTCGCGAAAGAAACGAAGTGAAGTATTCGTTCATCACCCAAAAGAAAAAGACCTACCCGGT
CGGCCTGATGTGCCGGCTATTGGGTGTCAGTCGCAGTGCTTACTATGACTATGAACAACGCCGCTGCAATTGCCCGGATGATCTACACCACAGGCAACTA
CTTAATGCTGTGCAGAATATTGCAAAATCATGCGATTACACCTATGGCAGTCGCAGGATGAAACGAGCGCTAAATGCCTTGGACTATCGGGTTAGTCGCT
GGAAGGCGAGAAGACTAATGCAAGAAGCCGGGATACAAGTGAAACACCGGAAGAAGTACAAGGTGACGACGGACAGCAATCACCCGTTGCCAGTGTTTGA
GAACCAATTGAATCGGCAATTTACGGTTGCCAGACCGGATCAAGTCTATGTTTGCGACATTACCTGCATTTGGACTCAGGAAGGCTGGCTGTATCTGGCG
GTTGTCATCGATTTGTTCTCCCGAAAGGTGGTGGGCTGGAGTATGAGCTCACGGATGAAAACAACACTGGTTTGTGATGCATTACGAATGGCGATCTGGC
TACGCCGGCCACCGCCTGGCCTGATCGTGCATTCGGATCGTGGGTCGCAGTATGCTAGCAAAGCATACCGCAATCTACTGAAAGCATACGGTTTTATTGG
CAGTATGAGTCGTCTGGGAAATTGTTGGGATAATGCGGTTGCGGAAAGTTTCTTTGGCAGCCTCAAGCAGGAACGCTGCCAATGGCGGCATTACCAAACC
CGCCATGCAGCACAGCAGGATATTTTGCAGTATATCGCCGTGTTTTATAATAACCAAAGACTGCATTCATACCTGGATTACAAAAGTCCGAATCAATATG
AAGCAGAAGCAGCAAAATCGATAAAAGCAGCTTAACTGAGGTGTCCGATTTTACTTGACCACGTTA
TGGATGCAATCAGCTTGGTACTGGATCAGGGATTAACGATAGCAGAAGCTGCCAGAAGCCTGGAAATCAGGGCTAACATGCTTGGGCGATGGATCAGGGA
GAGCCAGGCGGATACTAATGGTCAGGCATTCCGCGGTAATGGGAAACTGACACCGGAGCAGGAAGAAATCCGGCGGCTTAAACTAGAAAACAAGCAATTA
AAGCTTGAGCGGCAAATACTAAAGGAGGCGGCGGTCTTTTTCGCGAAAGAAACGAAGTGAAGTATTCGTTCATCACCCAAAAGAAAAAGACCTACCCGGT
CGGCCTGATGTGCCGGCTATTGGGTGTCAGTCGCAGTGCTTACTATGACTATGAACAACGCCGCTGCAATTGCCCGGATGATCTACACCACAGGCAACTA
CTTAATGCTGTGCAGAATATTGCAAAATCATGCGATTACACCTATGGCAGTCGCAGGATGAAACGAGCGCTAAATGCCTTGGACTATCGGGTTAGTCGCT
GGAAGGCGAGAAGACTAATGCAAGAAGCCGGGATACAAGTGAAACACCGGAAGAAGTACAAGGTGACGACGGACAGCAATCACCCGTTGCCAGTGTTTGA
GAACCAATTGAATCGGCAATTTACGGTTGCCAGACCGGATCAAGTCTATGTTTGCGACATTACCTGCATTTGGACTCAGGAAGGCTGGCTGTATCTGGCG
GTTGTCATCGATTTGTTCTCCCGAAAGGTGGTGGGCTGGAGTATGAGCTCACGGATGAAAACAACACTGGTTTGTGATGCATTACGAATGGCGATCTGGC
TACGCCGGCCACCGCCTGGCCTGATCGTGCATTCGGATCGTGGGTCGCAGTATGCTAGCAAAGCATACCGCAATCTACTGAAAGCATACGGTTTTATTGG
CAGTATGAGTCGTCTGGGAAATTGTTGGGATAATGCGGTTGCGGAAAGTTTCTTTGGCAGCCTCAAGCAGGAACGCTGCCAATGGCGGCATTACCAAACC
CGCCATGCAGCACAGCAGGATATTTTGCAGTATATCGCCGTGTTTTATAATAACCAAAGACTGCATTCATACCTGGATTACAAAAGTCCGAATCAATATG
AAGCAGAAGCAGCAAAATCGATAAAAGCAGCTTAACTGAGGTGTCCGATTTTACTTGACCACGTTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 64 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MEPGKHYTKEFKLDAISLVLDQGLTIAEAARSLEIRANMLGRWIRESQADTNGQAFRGNGKLTPEQEEIRRLKLENKQLKLERQILKEAAVFFAKETK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 927 bp | 308 aa | 309 | 1235 | + | No |
Description : Second part of the transposase
ORF sequence :
AANTKGGGGLFRERNEVKYSFITQKKKTYPVGLMCRLLGVSRSAYYDYEQRRCNCPDDLHHRQLLNAVQNIAKSCDYTYGSRRMKRALNALDYRVSRWKA
RRLMQEAGIQVKHRKKYKVTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITCIWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKTTLVCDALRMAIWLRR
PPPGLIVHSDRGSQYASKAYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDILQYIAVFYNNQRLHSYLDYKSPNQYEAE
AAKSIKAA
RRLMQEAGIQVKHRKKYKVTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITCIWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKTTLVCDALRMAIWLRR
PPPGLIVHSDRGSQYASKAYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDILQYIAVFYNNQRLHSYLDYKSPNQYEAE
AAKSIKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 390 aa | 64 | 1235 | + | Yes |
Chemistry : DDE
ORF sequence :
MEPGKHYTKEFKLDAISLVLDQGLTIAEAARSLEIRANMLGRWIRESQADTNGQAFRGNGKLTPEQEEIRRLKLENKQLKLERQILKEAAVFFRERNEVK
YSFITQKKKTYPVGLMCRLLGVSRSAYYDYEQRRCNCPDDLHHRQLLNAVQNIAKSCDYTYGSRRMKRALNALDYRVSRWKARRLMQEAGIQVKHRKKYK
VTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITCIWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKTTLVCDALRMAIWLRRPPPGLIVHSDRGSQYASK
AYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDILQYIAVFYNNQRLHSYLDYKSPNQYEAEAAKSIKAA
YSFITQKKKTYPVGLMCRLLGVSRSAYYDYEQRRCNCPDDLHHRQLLNAVQNIAKSCDYTYGSRRMKRALNALDYRVSRWKARRLMQEAGIQVKHRKKYK
VTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITCIWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKTTLVCDALRMAIWLRRPPPGLIVHSDRGSQYASK
AYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDILQYIAVFYNNQRLHSYLDYKSPNQYEAEAAKSIKAA
Blast result :
Comments
ISNieu3 is 84% (ORFA) and 84% (ORFB) aa similar ISNieu2. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Arp,D., Klotz,M., Norton,J., Stein,L., Sayavedra-Soto,L., Hommes,N. and Richardson,P. (2006) Direct submission GenBank.