ISShes1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008750 | ND | Shewanella sp. | Shewanella sp. W3-18-1 |
DNA section
IS Length : 1243 bp
Ends
IR Length : 26/35
IRL : TGATCTGATCCAATACTTTTGGACACCACGCTAAGTGAGTAAAATGACTT
IRR : TGATCTGATCCGCTAACTCTGGACACTTAACTAAGCACTGTGTTTCAGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGGCATCTC | TCTCCTTTCT | 0 |
DNA sequence
TGATCTGATCCAATACTTTTGGACACCACGCTAAGTGAGTAAAATGACTTAACGAGGTGAATATGACAACCCAAAAGACCCGCAAGACTCACACTGCTGA
ATTTAAAGCAGAAGCCCTTAAACTAGCCGAACGTGTCGGTGTTGCTGAAGCGGCTAGGCAACTCAAAATCTACGAATCACAACTTTATAACTGGCGCTCT
GCCATTGAAAAGAAATCGACAACCAGCCAACGCGAAGCAGAACTTGCTGCTGAAGTTGCCAAGTTAAAACGCCAGTTAGCTGACCAAGCCGAGGATTTAG
CCATCCTAAAAAAGGCGGCCACCTACTTCGCGAAGAATCAAAAGTAAACCGATTCGAATTTATGCTCGAACACCAGCACCAGTTCCGCCTAAAACCAATG
GCGGCGGTGCTTGGCGTGACGCGAAGTGGGTATTATGCCTGGCGAAAATCAAGCAAAGAACCGAGTGCGCGCTTGCTCAAACAGCACAGCCGTGATGAGC
AAATCAAAGCTTTTTTTGTCCAGAGCAAAGAGCGCTCAGGCGCTCGGCGTATTCAAGCCGACCTCGCGGAGGATGGGGAATGCGCTGATCTGAAAACCAT
TATGAACAGCATGACAAGGCAGAATTTAGTGGCTAAAGCTGCGCGTAAATTCAAAACCACAACCGATAGCGATCACCAGCTTCCGGTAGCGCCAAACCTA
TTGGAGCAGAACTTCGATACCACTGGCCCGAACCAAAAATGGGTCGGTGATATCACGTATCTGATGACAAGCGAAGGCTGGCTGTATCTGGCGGTGATTA
TTGATTTGTACTCGCGAGCTGTGATTGGCTGGTCAATGAATAATCGTATGACGGCTCAGTTGGCTTGTGATGCACTGACTATGGCATTGTTCCGCCGTGG
GTTCCCGAAAAATGTAATTGTTCACAGCGACCGCGGTAGCCAATATTGTTCACATGCCTATCGAAATCTGATTGAAAAACATCAACTTACCCAAAGTATG
AGCCGCAAAGCCTGCTGCTGGGACAATGCCTGTGCAGAGAGCTTCTTCCATTCACTGAAGGTTGAGGCGATTCAGTACGAGCCAATCATGGACCGGGAAA
CCATGCGACAGACCGTTTTTGAATACATAGAAGTTGATTACAACAAAACCAGAAGGCATAGTTCACTTGGCTATTTAAGCCCTGAGAATTTTGAACTGAA
ACACAGTGCTTAGTTAAGTGTCCAGAGTTAGCGGATCAGATCA
ATTTAAAGCAGAAGCCCTTAAACTAGCCGAACGTGTCGGTGTTGCTGAAGCGGCTAGGCAACTCAAAATCTACGAATCACAACTTTATAACTGGCGCTCT
GCCATTGAAAAGAAATCGACAACCAGCCAACGCGAAGCAGAACTTGCTGCTGAAGTTGCCAAGTTAAAACGCCAGTTAGCTGACCAAGCCGAGGATTTAG
CCATCCTAAAAAAGGCGGCCACCTACTTCGCGAAGAATCAAAAGTAAACCGATTCGAATTTATGCTCGAACACCAGCACCAGTTCCGCCTAAAACCAATG
GCGGCGGTGCTTGGCGTGACGCGAAGTGGGTATTATGCCTGGCGAAAATCAAGCAAAGAACCGAGTGCGCGCTTGCTCAAACAGCACAGCCGTGATGAGC
AAATCAAAGCTTTTTTTGTCCAGAGCAAAGAGCGCTCAGGCGCTCGGCGTATTCAAGCCGACCTCGCGGAGGATGGGGAATGCGCTGATCTGAAAACCAT
TATGAACAGCATGACAAGGCAGAATTTAGTGGCTAAAGCTGCGCGTAAATTCAAAACCACAACCGATAGCGATCACCAGCTTCCGGTAGCGCCAAACCTA
TTGGAGCAGAACTTCGATACCACTGGCCCGAACCAAAAATGGGTCGGTGATATCACGTATCTGATGACAAGCGAAGGCTGGCTGTATCTGGCGGTGATTA
TTGATTTGTACTCGCGAGCTGTGATTGGCTGGTCAATGAATAATCGTATGACGGCTCAGTTGGCTTGTGATGCACTGACTATGGCATTGTTCCGCCGTGG
GTTCCCGAAAAATGTAATTGTTCACAGCGACCGCGGTAGCCAATATTGTTCACATGCCTATCGAAATCTGATTGAAAAACATCAACTTACCCAAAGTATG
AGCCGCAAAGCCTGCTGCTGGGACAATGCCTGTGCAGAGAGCTTCTTCCATTCACTGAAGGTTGAGGCGATTCAGTACGAGCCAATCATGGACCGGGAAA
CCATGCGACAGACCGTTTTTGAATACATAGAAGTTGATTACAACAAAACCAGAAGGCATAGTTCACTTGGCTATTTAAGCCCTGAGAATTTTGAACTGAA
ACACAGTGCTTAGTTAAGTGTCCAGAGTTAGCGGATCAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 63 | 347 | + | No |
Description : First part of the transposase
ORF sequence :
MTTQKTRKTHTAEFKAEALKLAERVGVAEAARQLKIYESQLYNWRSAIEKKSTTSQREAELAAEVAKLKRQLADQAEDLAILKKAATYFAKNQK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 284 | 1213 | + | No |
Description : Second part of the transposase
ORF sequence :
PSRGFSHPKKGGHLLREESKVNRFEFMLEHQHQFRLKPMAAVLGVTRSGYYAWRKSSKEPSARLLKQHSRDEQIKAFFVQSKERSGARRIQADLAEDGEC
ADLKTIMNSMTRQNLVAKAARKFKTTTDSDHQLPVAPNLLEQNFDTTGPNQKWVGDITYLMTSEGWLYLAVIIDLYSRAVIGWSMNNRMTAQLACDALTM
ALFRRGFPKNVIVHSDRGSQYCSHAYRNLIEKHQLTQSMSRKACCWDNACAESFFHSLKVEAIQYEPIMDRETMRQTVFEYIEVDYNKTRRHSSLGYLSP
ENFELKHSA
ADLKTIMNSMTRQNLVAKAARKFKTTTDSDHQLPVAPNLLEQNFDTTGPNQKWVGDITYLMTSEGWLYLAVIIDLYSRAVIGWSMNNRMTAQLACDALTM
ALFRRGFPKNVIVHSDRGSQYCSHAYRNLIEKHQLTQSMSRKACCWDNACAESFFHSLKVEAIQYEPIMDRETMRQTVFEYIEVDYNKTRRHSSLGYLSP
ENFELKHSA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 63 | 1213 | + | Yes |
Chemistry : DDE
ORF sequence :
MTTQKTRKTHTAEFKAEALKLAERVGVAEAARQLKIYESQLYNWRSAIEKKSTTSQREAELAAEVAKLKRQLADQAEDLAILKKGGHLLREESKVNRFEF
MLEHQHQFRLKPMAAVLGVTRSGYYAWRKSSKEPSARLLKQHSRDEQIKAFFVQSKERSGARRIQADLAEDGECADLKTIMNSMTRQNLVAKAARKFKTT
TDSDHQLPVAPNLLEQNFDTTGPNQKWVGDITYLMTSEGWLYLAVIIDLYSRAVIGWSMNNRMTAQLACDALTMALFRRGFPKNVIVHSDRGSQYCSHAY
RNLIEKHQLTQSMSRKACCWDNACAESFFHSLKVEAIQYEPIMDRETMRQTVFEYIEVDYNKTRRHSSLGYLSPENFELKHSA
MLEHQHQFRLKPMAAVLGVTRSGYYAWRKSSKEPSARLLKQHSRDEQIKAFFVQSKERSGARRIQADLAEDGECADLKTIMNSMTRQNLVAKAARKFKTT
TDSDHQLPVAPNLLEQNFDTTGPNQKWVGDITYLMTSEGWLYLAVIIDLYSRAVIGWSMNNRMTAQLACDALTMALFRRGFPKNVIVHSDRGSQYCSHAY
RNLIEKHQLTQSMSRKACCWDNACAESFFHSLKVEAIQYEPIMDRETMRQTVFEYIEVDYNKTRRHSSLGYLSPENFELKHSA
Blast result :
Comments
ISShes1 is 85% (ORFA) and 76% (ORFB) aa similar to ISVpa2. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A., Tiedje,J. and Richardson,P. (2007) Direct submission Genbank.