ISRba6
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005027 | ND | Rhodopirellula baltica | Rhodopirellula baltica SH 1 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 34/36
IRL : TGGAATTGCCCACGAAAAGTTGACAGTGGGCCGGCGCACGTCAGACTTCT
IRR : TGGAATTGCCCACGAAAAGTTGACAGTGGACCGGTCTTAAGGGAAAGGTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACGCAGACC | GTC | AGGGAGCGGC | 3 |
DNA sequence
TGGAATTGCCCACGAAAAGTTGACAGTGGGCCGGCGCACGTCAGACTTCTCTGACAGGAGAACCGATCCATGGACAAACGTCGAACATTTAGCCGCGAAT
ACAAGCTGGCCGCAGTCAAGAAAGTCATCGAACAAGGCTTGTCGTACACCGCTGTCGCTAAAGACTTGGGGATCGGGGACAGCTTGATTCGCAAGTGGAA
GAAGTCTTTTGACGAAGACGGAACATTCCAGGCCGAAGTAGTTGGTAGCCAATCCATTGAAGCCGAGCTGAGACGACTTCGCGAAGAGAATCGTCAACTC
AAGATGGAACGCGACATTTTAAAAAAAGCGACGGCATTCTTCGCCAAAGAAAGTCACTGAGGTTGAAGTTCATTGGAGAGTGCCGCGATCGCTGGCCGAT
CGCAGTGCTCTGCCGAACCCTCGAAGTCACTCGCGCCGCTTATTACCGATTCGCCGGTCGCGGTCCCACAGCCACCGAGATCAAGCAAACCCAAATCATT
CAAGCCGTCAAGGAAATCCGACTGGAAAAACATCACGATGCGTATGGAAGCCCGCGAATGCAACGAGCAATAGTCAAACGCGGTGTGGTGTGCTGCCGAA
ATACCGTCGCCAAATGCATGCGTCATGCGGGAATACAAGCCAATCGCCGCACCAAATTCAGAATATCGACCACTGACTCCAATCATGATCAGCCCATCGC
CTCAAATTTGCTTGGCCAAAACTTCACGACCGAGGCAATCAATCGCGTCTGGCTAACGGACATCACCTACATCCCAACCCAAGAAGGCTCCACTTACCTC
TGTGCATTCGTTGACCTGCATTCCCGCAAGATTGTCAGCTGGAAAACGAGCCGGAACATGGATTCGGAATTGGTGGTCGGGGCATTCGATCAAGCACTTA
CTTTTCGCAAGCCAAACGCGGGCCTGATCGTTCACAGCGATCGTGGCTCCCAATTCGCGAGCGATCATTTCCGCAGACGCCTGGCAGCCAGTGGGCTAGT
TCAAAGCATGAGCCGTCGCGGGAACTGCTACGACAACGCACCGATGGAATCGTTCTTCAAGAGTTACAAAACCGAGGAAGCACAGCAGATTTACGACACG
CACGAACACGCCACACGCGGCGTATCTGACTACATCGAACGATTTTACAACCCTCATCGCTTGCACTCGTCGCTGGGCTACCTCAGTCCAATCGATTTCG
AGCAAGCGATCAAAGAACCGTCACTCGTAAGTGAGTCCTGACTCACAGGACTCACCGTTTACCTTTCCCTTAAGACCGGTCCACTGTCAACTTTTCGTGG
GCAATTCCA
ACAAGCTGGCCGCAGTCAAGAAAGTCATCGAACAAGGCTTGTCGTACACCGCTGTCGCTAAAGACTTGGGGATCGGGGACAGCTTGATTCGCAAGTGGAA
GAAGTCTTTTGACGAAGACGGAACATTCCAGGCCGAAGTAGTTGGTAGCCAATCCATTGAAGCCGAGCTGAGACGACTTCGCGAAGAGAATCGTCAACTC
AAGATGGAACGCGACATTTTAAAAAAAGCGACGGCATTCTTCGCCAAAGAAAGTCACTGAGGTTGAAGTTCATTGGAGAGTGCCGCGATCGCTGGCCGAT
CGCAGTGCTCTGCCGAACCCTCGAAGTCACTCGCGCCGCTTATTACCGATTCGCCGGTCGCGGTCCCACAGCCACCGAGATCAAGCAAACCCAAATCATT
CAAGCCGTCAAGGAAATCCGACTGGAAAAACATCACGATGCGTATGGAAGCCCGCGAATGCAACGAGCAATAGTCAAACGCGGTGTGGTGTGCTGCCGAA
ATACCGTCGCCAAATGCATGCGTCATGCGGGAATACAAGCCAATCGCCGCACCAAATTCAGAATATCGACCACTGACTCCAATCATGATCAGCCCATCGC
CTCAAATTTGCTTGGCCAAAACTTCACGACCGAGGCAATCAATCGCGTCTGGCTAACGGACATCACCTACATCCCAACCCAAGAAGGCTCCACTTACCTC
TGTGCATTCGTTGACCTGCATTCCCGCAAGATTGTCAGCTGGAAAACGAGCCGGAACATGGATTCGGAATTGGTGGTCGGGGCATTCGATCAAGCACTTA
CTTTTCGCAAGCCAAACGCGGGCCTGATCGTTCACAGCGATCGTGGCTCCCAATTCGCGAGCGATCATTTCCGCAGACGCCTGGCAGCCAGTGGGCTAGT
TCAAAGCATGAGCCGTCGCGGGAACTGCTACGACAACGCACCGATGGAATCGTTCTTCAAGAGTTACAAAACCGAGGAAGCACAGCAGATTTACGACACG
CACGAACACGCCACACGCGGCGTATCTGACTACATCGAACGATTTTACAACCCTCATCGCTTGCACTCGTCGCTGGGCTACCTCAGTCCAATCGATTTCG
AGCAAGCGATCAAAGAACCGTCACTCGTAAGTGAGTCCTGACTCACAGGACTCACCGTTTACCTTTCCCTTAAGACCGGTCCACTGTCAACTTTTCGTGG
GCAATTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 70 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MDKRRTFSREYKLAAVKKVIEQGLSYTAVAKDLGIGDSLIRKWKKSFDEDGTFQAEVVGSQSIEAELRRLREENRQLKMERDILKKATAFFAKESH
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 981 bp | 326 aa | 261 | 1241 | + | No |
Description : Second part of the transposase
ORF sequence :
SRAETTSRRESSTQDGTRHFKKSDGILRQRKSLRLKFIGECRDRWPIAVLCRTLEVTRAAYYRFAGRGPTATEIKQTQIIQAVKEIRLEKHHDAYGSPRM
QRAIVKRGVVCCRNTVAKCMRHAGIQANRRTKFRISTTDSNHDQPIASNLLGQNFTTEAINRVWLTDITYIPTQEGSTYLCAFVDLHSRKIVSWKTSRNM
DSELVVGAFDQALTFRKPNAGLIVHSDRGSQFASDHFRRRLAASGLVQSMSRRGNCYDNAPMESFFKSYKTEEAQQIYDTHEHATRGVSDYIERFYNPHR
LHSSLGYLSPIDFEQAIKEPSLVSES
QRAIVKRGVVCCRNTVAKCMRHAGIQANRRTKFRISTTDSNHDQPIASNLLGQNFTTEAINRVWLTDITYIPTQEGSTYLCAFVDLHSRKIVSWKTSRNM
DSELVVGAFDQALTFRKPNAGLIVHSDRGSQFASDHFRRRLAASGLVQSMSRRGNCYDNAPMESFFKSYKTEEAQQIYDTHEHATRGVSDYIERFYNPHR
LHSSLGYLSPIDFEQAIKEPSLVSES
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 390 aa | 70 | 1241 | + | Yes |
Chemistry : DDE
ORF sequence :
MDKRRTFSREYKLAAVKKVIEQGLSYTAVAKDLGIGDSLIRKWKKSFDEDGTFQAEVVGSQSIEAELRRLREENRQLKMERDILKKSDGILRQRKSLRLK
FIGECRDRWPIAVLCRTLEVTRAAYYRFAGRGPTATEIKQTQIIQAVKEIRLEKHHDAYGSPRMQRAIVKRGVVCCRNTVAKCMRHAGIQANRRTKFRIS
TTDSNHDQPIASNLLGQNFTTEAINRVWLTDITYIPTQEGSTYLCAFVDLHSRKIVSWKTSRNMDSELVVGAFDQALTFRKPNAGLIVHSDRGSQFASDH
FRRRLAASGLVQSMSRRGNCYDNAPMESFFKSYKTEEAQQIYDTHEHATRGVSDYIERFYNPHRLHSSLGYLSPIDFEQAIKEPSLVSES
FIGECRDRWPIAVLCRTLEVTRAAYYRFAGRGPTATEIKQTQIIQAVKEIRLEKHHDAYGSPRMQRAIVKRGVVCCRNTVAKCMRHAGIQANRRTKFRIS
TTDSNHDQPIASNLLGQNFTTEAINRVWLTDITYIPTQEGSTYLCAFVDLHSRKIVSWKTSRNMDSELVVGAFDQALTFRKPNAGLIVHSDRGSQFASDH
FRRRLAASGLVQSMSRRGNCYDNAPMESFFKSYKTEEAQQIYDTHEHATRGVSDYIERFYNPHRLHSSLGYLSPIDFEQAIKEPSLVSES
Blast result :
Comments
ISRba6 is 59 % (ORFA) aa similar to ISStau1 and 63% (ORFB) aa similar to ISCce3. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Gloeckner,F.O., Kube,M., Bauer,M., Teeling,H., Lombardot,T., Ludwig,W., Gade,D., Beck,A., Borzym,K., Heitmann,K., Rabus,R., Schlesner,H., Amann,R. and Reinhardt,R. (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (14), 8298-8303