ISNisp1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAMY01000042 | ND | Nitrobacter sp. | Nitrobacter sp. Nb-311A |
DNA section
IS Length : 1208 bp
Ends
IR Length : 24/46
IRL : TGGAGGGACTTTCTGGTAGCGCTGGACGCAACAGGAGGACCCGATGGGCA
IRR : TGGAGAGGCTTTCACTCTGAGATGGCCATTGTGGCCTCGAATGATGCCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGGAAAAAA | CTAAACCCGGG | 0 |
DNA sequence
TGGAGGGACTTTCTGGTAGCGCTGGACGCAACAGGAGGACCCGATGGGCAAACGCGAGCGACGGATTTTCACGGAAGATTTCAAGAAAGAAGCGGTTCGG
CTGACAGAAACCAGTGGCCGAACGATTGGGCAAGTTGCCGATGATCTGGGGATCGGGTTGTCCAGCCTGACGCGGTGGAGGCGGCAATACCGGGAAGCCG
ATCTGCTGGCCGGCCCGCATGAGGACGCGGCCAAGGAACTGGCACGGCTTCGCAAAGAGAACGAACTCCTTCGCCAAGAGCGCGAGATCCTGAAGCGGGC
CGCAACTTTTTTCGCCAAGGAGGGAAGTCGATGAGGTTCGAGCTCATTGATCAGGCGAAGAAGGAATTCCCCGTGCATCGCCTGTGCGACGTTCTTGGTG
TCAGCGAGAGCGGATATTTTGCCTGGAAGGGTCGTCCTGCCAGTCGCCGGCAGGGTGAGGACATGGTCCTGCTTGCGCATATCCGTGCGCAGTTCTCGAC
GTCCAGCGAGACCTATGGCGCACCGCGCATGCACGCCGAACTCACCGAAGAGGGGCTTGCTGTCGGGCGGCATCGCGTTGCTCGGCTCATGCGCGACAAT
GGTCTGAAAGCCCTGCAGAAGCGCCGCTACAAGAAGACCACTGATAGTTCCCATGGCAGCCCAGTCGCCGCCAACCTCCTCGATCAGGATTTTGCTTGCG
ACCGCCCCGATCAGAAGTGGGGCGCTGATATCAGCTATATCTGGACGGCCGAGGGTTGGCTCTATCTGGCCATCGTGCTCGATCTCTACTCACGGCGTAT
CGTCGGTTGGGCAACGAGCGATCGGTTGAAGAAGGACCTGGCCCTCAATGCACTACGGCGCGCCATTACCCTTCGCAATCCGCCGTCCGGCCTAATCCAA
CACACCGATCGCGGCAGCCAATACTGCTCACACGATTATCAGCGGCTCCTGAAAGCGCACGACATCATCCCGTCGATGAGCGGAAAGGGCAATTGCTACG
ATAACGCGATGGTGGAAACGGTGTTCAAGACGATCAAGAGTGAGCTGGTCTGGAGAACGATCTTCCAGACCCGCGCCGCCGCCGAATGGGCCCTTGGCCG
ATACATCGACGGCTTCTACAATCCACGTCGACGACATTCCGCTCTGGGATACAAATCGCCGGCATCATTCGAGGCCACAATGGCCATCTCAGAGTGAAAG
CCTCTCCA
CTGACAGAAACCAGTGGCCGAACGATTGGGCAAGTTGCCGATGATCTGGGGATCGGGTTGTCCAGCCTGACGCGGTGGAGGCGGCAATACCGGGAAGCCG
ATCTGCTGGCCGGCCCGCATGAGGACGCGGCCAAGGAACTGGCACGGCTTCGCAAAGAGAACGAACTCCTTCGCCAAGAGCGCGAGATCCTGAAGCGGGC
CGCAACTTTTTTCGCCAAGGAGGGAAGTCGATGAGGTTCGAGCTCATTGATCAGGCGAAGAAGGAATTCCCCGTGCATCGCCTGTGCGACGTTCTTGGTG
TCAGCGAGAGCGGATATTTTGCCTGGAAGGGTCGTCCTGCCAGTCGCCGGCAGGGTGAGGACATGGTCCTGCTTGCGCATATCCGTGCGCAGTTCTCGAC
GTCCAGCGAGACCTATGGCGCACCGCGCATGCACGCCGAACTCACCGAAGAGGGGCTTGCTGTCGGGCGGCATCGCGTTGCTCGGCTCATGCGCGACAAT
GGTCTGAAAGCCCTGCAGAAGCGCCGCTACAAGAAGACCACTGATAGTTCCCATGGCAGCCCAGTCGCCGCCAACCTCCTCGATCAGGATTTTGCTTGCG
ACCGCCCCGATCAGAAGTGGGGCGCTGATATCAGCTATATCTGGACGGCCGAGGGTTGGCTCTATCTGGCCATCGTGCTCGATCTCTACTCACGGCGTAT
CGTCGGTTGGGCAACGAGCGATCGGTTGAAGAAGGACCTGGCCCTCAATGCACTACGGCGCGCCATTACCCTTCGCAATCCGCCGTCCGGCCTAATCCAA
CACACCGATCGCGGCAGCCAATACTGCTCACACGATTATCAGCGGCTCCTGAAAGCGCACGACATCATCCCGTCGATGAGCGGAAAGGGCAATTGCTACG
ATAACGCGATGGTGGAAACGGTGTTCAAGACGATCAAGAGTGAGCTGGTCTGGAGAACGATCTTCCAGACCCGCGCCGCCGCCGAATGGGCCCTTGGCCG
ATACATCGACGGCTTCTACAATCCACGTCGACGACATTCCGCTCTGGGATACAAATCGCCGGCATCATTCGAGGCCACAATGGCCATCTCAGAGTGAAAG
CCTCTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 289 bp | 96 aa | 46 | 334 | + | No |
Description : First part of the transposase
ORF sequence :
MGKRERRIFTEDFKKEAVRLTETSGRTIGQVADDLGIGLSSLTRWRRQYREADLLAGPHEDAAKELARLRKENELLRQEREILKRAATFFAKEGSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 975 bp | 324 aa | 223 | 1197 | + | No |
Description : Second part of the transposase
ORF sequence :
GRGQGTGTASQRERTPSPRARDPEAGRNFFRQGGKSMRFELIDQAKKEFPVHRLCDVLGVSESGYFAWKGRPASRRQGEDMVLLAHIRAQFSTSSETYGA
PRMHAELTEEGLAVGRHRVARLMRDNGLKALQKRRYKKTTDSSHGSPVAANLLDQDFACDRPDQKWGADISYIWTAEGWLYLAIVLDLYSRRIVGWATSD
RLKKDLALNALRRAITLRNPPSGLIQHTDRGSQYCSHDYQRLLKAHDIIPSMSGKGNCYDNAMVETVFKTIKSELVWRTIFQTRAAAEWALGRYIDGFYN
PRRRHSALGYKSPASFEATMAISE
PRMHAELTEEGLAVGRHRVARLMRDNGLKALQKRRYKKTTDSSHGSPVAANLLDQDFACDRPDQKWGADISYIWTAEGWLYLAIVLDLYSRRIVGWATSD
RLKKDLALNALRRAITLRNPPSGLIQHTDRGSQYCSHDYQRLLKAHDIIPSMSGKGNCYDNAMVETVFKTIKSELVWRTIFQTRAAAEWALGRYIDGFYN
PRRRHSALGYKSPASFEATMAISE
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1152 bp | 384 aa | 46 | 1197 | + | Yes |
Chemistry : DDE
ORF sequence :
MGKRERRIFTEDFKKEAVRLTETSGRTIGQVADDLGIGLSSLTRWRRQYREADLLAGPHEDAAKELARLRKENELLRQEREILKRAATFFRQGGKSMRFE
LIDQAKKEFPVHRLCDVLGVSESGYFAWKGRPASRRQGEDMVLLAHIRAQFSTSSETYGAPRMHAELTEEGLAVGRHRVARLMRDNGLKALQKRRYKKTT
DSSHGSPVAANLLDQDFACDRPDQKWGADISYIWTAEGWLYLAIVLDLYSRRIVGWATSDRLKKDLALNALRRAITLRNPPSGLIQHTDRGSQYCSHDYQ
RLLKAHDIIPSMSGKGNCYDNAMVETVFKTIKSELVWRTIFQTRAAAEWALGRYIDGFYNPRRRHSALGYKSPASFEATMAISE
LIDQAKKEFPVHRLCDVLGVSESGYFAWKGRPASRRQGEDMVLLAHIRAQFSTSSETYGAPRMHAELTEEGLAVGRHRVARLMRDNGLKALQKRRYKKTT
DSSHGSPVAANLLDQDFACDRPDQKWGADISYIWTAEGWLYLAIVLDLYSRRIVGWATSDRLKKDLALNALRRAITLRNPPSGLIQHTDRGSQYCSHDYQ
RLLKAHDIIPSMSGKGNCYDNAMVETVFKTIKSELVWRTIFQTRAAAEWALGRYIDGFYNPRRRHSALGYKSPASFEATMAISE
Blast result :
Comments
Genome in process.
ISNisp1 is 84% (ORFA) and 81% (ORFB) aa similar to IS1953. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
ISNisp1 is 84% (ORFA) and 81% (ORFB) aa similar to IS1953. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Waterbury,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. And Venter,J.C. (2006) Direct Submission GenBank.