ISBlma4
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AANZ01000005 | ND | Blastopirellula marina | Blastopirellula marina DSM 3645 |
DNA section
IS Length : 1307 bp
Ends
IR Length : 32/33
IRL : TGGAATTGCCCATGAAAAGTTGACAGTGGATCGAGAGATGTCAGACTTCT
IRR : TGGAATTGCCCACGAAAAGTTGACAGTGGATCGGTCTTAACGGGCAGGTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAACGAAGGA | AAGTTCTAGG | 0 |
DNA sequence
TGGAATTGCCCATGAAAAGTTGACAGTGGATCGAGAGATGTCAGACTTCTCTGACAGGAGATCCGATTCATGCCCAAGCGTCGAACATTCAGCCGAGAAT
TCAAACTGGCCGCTGTCAAGAAGATCACCGAACAAGGGCTGTCGTACGCAGCTGTCGCCAAAGACTTGAGTGTGGGTGAAACCTTGCTTCGTAAATGGAA
GAAGTCCCTGGATGAAACAGGGGCCCTTCCGGTCGCCCCCGCTGGCGGCCAGTCGGTCGAGGCTGAGTTGAAACGGCTCCGTGAGGAGAACCGTCAACTC
AAGATGGAACGCGACATTTTAAAAAAAGCGACGGCGTTCTTCGCCAAAGAAAGCAACTGAGGCTCAAGTTCATTGACAGGCATCACGATCGGTGGCCGAT
CGCGGTGCTCTGTCGAGCTCTCGAAGTGACGCGTGCGGCGTACTACCGATTCACTCATCTCGATGTCACGGCGACCGAGACAAAACAAAAGCAAATCACT
CGCGCCATTCAAGAGGTTCATTCCGAGAAGCATTTCGATGCTTACGGAAGTCCAAGAATGCACCGAGAACTGATAAGTCGCGGTATTGAATGCTGCCGAA
ACACGGTCGCAAAATGCATGCGGATGGCTGGAATCCAGGCAAATCGCCGCACCAAATTCAGAATTTCGACAACCGACTCCAACCACGACCAACCGATTGC
CCCCAATCTGCTTGACCAAAACTTCTCCACGGAAACCATCAATCAAGTTTGGCTCACGGATATCACTTACCTACCGACCAAAGAGGGATTCACTCACTTG
TGCGCTTTTGTCGACCTGCATTCACGCAAGATCATAGGTTGGAAGACCAGTCGCAACATCGATTCGGAGTTGGTGGTCGCGGCGTTGGACCAATCACTTG
CGTTTCGCTCACCGAGAGCCGGACTGATAGTTCATAGTGATCGCGGTTCGCAGTATGCCAGCGAACATTTTCGCAGCCGGTTAGCCGTCCACGAAATCGT
TCAGAGCATGAGTCGTCGCGGCAACTGCTACGACAACGCGCCGATGGAATCCTTCTTCAAGAGCTACAAGACCGAGGAAGCCCAGATGATCTACGAAACG
CACGAACACGCCACACGTGGCGCATCGGACTACATCGAACGCTTTTACAATCCTCGTCGCCTGCACTCGTCACTCGATTACCGAAGTCCAATCGAGTTCG
AGCAAGCGATCAAAGAACCGTTACTCGTGGGCGCGCTCTGATCCAGGCCGCGCCAATTACCTGCCCGTTAAGACCGATCCACTGTCAACTTTTCGTGGGC
AATTCCA
TCAAACTGGCCGCTGTCAAGAAGATCACCGAACAAGGGCTGTCGTACGCAGCTGTCGCCAAAGACTTGAGTGTGGGTGAAACCTTGCTTCGTAAATGGAA
GAAGTCCCTGGATGAAACAGGGGCCCTTCCGGTCGCCCCCGCTGGCGGCCAGTCGGTCGAGGCTGAGTTGAAACGGCTCCGTGAGGAGAACCGTCAACTC
AAGATGGAACGCGACATTTTAAAAAAAGCGACGGCGTTCTTCGCCAAAGAAAGCAACTGAGGCTCAAGTTCATTGACAGGCATCACGATCGGTGGCCGAT
CGCGGTGCTCTGTCGAGCTCTCGAAGTGACGCGTGCGGCGTACTACCGATTCACTCATCTCGATGTCACGGCGACCGAGACAAAACAAAAGCAAATCACT
CGCGCCATTCAAGAGGTTCATTCCGAGAAGCATTTCGATGCTTACGGAAGTCCAAGAATGCACCGAGAACTGATAAGTCGCGGTATTGAATGCTGCCGAA
ACACGGTCGCAAAATGCATGCGGATGGCTGGAATCCAGGCAAATCGCCGCACCAAATTCAGAATTTCGACAACCGACTCCAACCACGACCAACCGATTGC
CCCCAATCTGCTTGACCAAAACTTCTCCACGGAAACCATCAATCAAGTTTGGCTCACGGATATCACTTACCTACCGACCAAAGAGGGATTCACTCACTTG
TGCGCTTTTGTCGACCTGCATTCACGCAAGATCATAGGTTGGAAGACCAGTCGCAACATCGATTCGGAGTTGGTGGTCGCGGCGTTGGACCAATCACTTG
CGTTTCGCTCACCGAGAGCCGGACTGATAGTTCATAGTGATCGCGGTTCGCAGTATGCCAGCGAACATTTTCGCAGCCGGTTAGCCGTCCACGAAATCGT
TCAGAGCATGAGTCGTCGCGGCAACTGCTACGACAACGCGCCGATGGAATCCTTCTTCAAGAGCTACAAGACCGAGGAAGCCCAGATGATCTACGAAACG
CACGAACACGCCACACGTGGCGCATCGGACTACATCGAACGCTTTTACAATCCTCGTCGCCTGCACTCGTCACTCGATTACCGAAGTCCAATCGAGTTCG
AGCAAGCGATCAAAGAACCGTTACTCGTGGGCGCGCTCTGATCCAGGCCGCGCCAATTACCTGCCCGTTAAGACCGATCCACTGTCAACTTTTCGTGGGC
AATTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 70 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MPKRRTFSREFKLAAVKKITEQGLSYAAVAKDLSVGETLLRKWKKSLDETGALPVAPAGGQSVEAELKRLREENRQLKMERDILKKATAFFAKESN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 957 bp | 318 aa | 285 | 1241 | + | No |
Description : Second part of the transposase
ORF sequence :
GEPSTQDGTRHFKKSDGVLRQRKQLRLKFIDRHHDRWPIAVLCRALEVTRAAYYRFTHLDVTATETKQKQITRAIQEVHSEKHFDAYGSPRMHRELISRG
IECCRNTVAKCMRMAGIQANRRTKFRISTTDSNHDQPIAPNLLDQNFSTETINQVWLTDITYLPTKEGFTHLCAFVDLHSRKIIGWKTSRNIDSELVVAA
LDQSLAFRSPRAGLIVHSDRGSQYASEHFRSRLAVHEIVQSMSRRGNCYDNAPMESFFKSYKTEEAQMIYETHEHATRGASDYIERFYNPRRLHSSLDYR
SPIEFEQAIKEPLLVGAL
IECCRNTVAKCMRMAGIQANRRTKFRISTTDSNHDQPIAPNLLDQNFSTETINQVWLTDITYLPTKEGFTHLCAFVDLHSRKIIGWKTSRNIDSELVVAA
LDQSLAFRSPRAGLIVHSDRGSQYASEHFRSRLAVHEIVQSMSRRGNCYDNAPMESFFKSYKTEEAQMIYETHEHATRGASDYIERFYNPRRLHSSLDYR
SPIEFEQAIKEPLLVGAL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 390 aa | 70 | 1241 | + | Yes |
Chemistry : DDE
ORF sequence :
MPKRRTFSREFKLAAVKKITEQGLSYAAVAKDLSVGETLLRKWKKSLDETGALPVAPAGGQSVEAELKRLREENRQLKMERDILKKSDGVLRQRKQLRLK
FIDRHHDRWPIAVLCRALEVTRAAYYRFTHLDVTATETKQKQITRAIQEVHSEKHFDAYGSPRMHRELISRGIECCRNTVAKCMRMAGIQANRRTKFRIS
TTDSNHDQPIAPNLLDQNFSTETINQVWLTDITYLPTKEGFTHLCAFVDLHSRKIIGWKTSRNIDSELVVAALDQSLAFRSPRAGLIVHSDRGSQYASEH
FRSRLAVHEIVQSMSRRGNCYDNAPMESFFKSYKTEEAQMIYETHEHATRGASDYIERFYNPRRLHSSLDYRSPIEFEQAIKEPLLVGAL
FIDRHHDRWPIAVLCRALEVTRAAYYRFTHLDVTATETKQKQITRAIQEVHSEKHFDAYGSPRMHRELISRGIECCRNTVAKCMRMAGIQANRRTKFRIS
TTDSNHDQPIAPNLLDQNFSTETINQVWLTDITYLPTKEGFTHLCAFVDLHSRKIIGWKTSRNIDSELVVAALDQSLAFRSPRAGLIVHSDRGSQYASEH
FRSRLAVHEIVQSMSRRGNCYDNAPMESFFKSYKTEEAQMIYETHEHATRGASDYIERFYNPRRLHSSLDYRSPIEFEQAIKEPLLVGAL
Blast result :
Comments
ISBlma4 is 61 % (ORFA) aa similar to ISStau1 and 57% (ORFB) aa similar to ISCausp1. The third ORF is a putative ORFAB transposase reconstructed in sillico by possible -1 frameshift.
References
1] Amann,R., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank